Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 604,753 A→C 5.3% intergenic (‑90/+18) ybdG ← / ← nfsB mechanosensitive channel protein, miniconductance/dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913604,7530AC 5.3% 284.4 / 2.4 100intergenic (‑90/+18)ybdG/nfsBmechanosensitive channel protein, miniconductance/dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
Reads supporting (aligned to +/- strand):  ref base A (43/51);  new base C (3/3);  total (46/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GGTGTGATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGACCAAATT  >  NC_000913/604607‑604901
                                                                                                                                                   |                                                                                                                                                     
ggtgTGATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATg                                                                                                                                                     >  5:281027/1‑149 (MQ=255)
  tgtgATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAAATAGCCGGGCAGATGc                                                                                                                                                    >  7:131874/1‑149 (MQ=255)
   gtgATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGccc                                                                                                                                                  >  4:268991/1‑149 (MQ=255)
   gtgATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGccc                                                                                                                                                  >  5:77503/1‑149 (MQ=255)
   gtgATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGAAAATAGCCGGGCAGATGccc                                                                                                                                                  >  3:316027/1‑149 (MQ=255)
      atcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCCGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCgg                                                                                                                                                >  1:296002/1‑148 (MQ=255)
      atcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCATCTGCCCGGc                                                                                                                                               >  6:343186/1‑149 (MQ=255)
       tcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGc                                                                                                                                               >  5:363802/1‑148 (MQ=255)
       tcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGc                                                                                                                                               >  7:303824/1‑148 (MQ=255)
         gatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGc                                                                                                                                               >  5:97666/1‑146 (MQ=255)
         gatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCaa                                                                                                                                             <  5:376369/148‑1 (MQ=255)
          atcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCaa                                                                                                                                             >  8:304072/1‑147 (MQ=255)
              cAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAgagaga                                                                                                                                       <  5:120694/149‑1 (MQ=255)
               aaTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACCGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGaa                                                                                                                                      <  2:138702/149‑1 (MQ=255)
               aaTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCATCTGCCCGGctat                                                                                                                                            <  5:343186/143‑4 (MQ=255)
               aaTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAaga                                                                                                                                           <  1:305431/144‑1 (MQ=255)
               aaTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGaa                                                                                                                                      >  3:142783/1‑149 (MQ=255)
               aaTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGaa                                                                                                                                      >  2:262743/1‑149 (MQ=255)
               aaTACCCGCTAAATCTTCAACCTCGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGaa                                                                                                                                      <  1:115176/149‑1 (MQ=255)
                aTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAAt                                                                                                                                     <  7:364254/149‑1 (MQ=255)
                   cccGCTAATTGTTCATGCTGGGTTATTAAATCCTGCATATCGTCTCCTTTATAGAACTGCAGCCTATGATGACGGGTGAAACAGGGTTTTTCAAATCTTGAGATTCTGAGAGGAAATAGCCGGGCAGATGCCCGGGAagtgagtttta                                                                                                                                   <  8:226544/148‑6 (MQ=255)
                   cccGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACCACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTa                                                                                                                                   <  7:332897/148‑1 (MQ=255)
                    ccGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACATGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGCTGCCCGGCAAGAGCGAATTaca                                                                                                                                 >  8:328008/1‑149 (MQ=255)
                       cTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACtt                                                                                                                              <  6:77503/149‑1 (MQ=255)
                       cTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTATATACAACAGCAGCCTATGAAGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACtt                                                                                                                              >  1:34207/1‑149 (MQ=255)
                          aaTCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGcc                                                                                                                                                   >  7:335947/1‑125 (MQ=255)
                          aaTCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGcc                                                                                                                                                   <  8:335947/125‑1 (MQ=255)
                          aaTCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCg                                                                                                                            <  1:329781/148‑1 (MQ=255)
                                cAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTaa                                                                                                                       <  5:56872/147‑1 (MQ=255)
                                cAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTa                                                                                                                        <  2:296002/146‑1 (MQ=255)
                                      gggATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGt                                                                                                               >  3:35188/1‑149 (MQ=255)
                                       ggATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCTGGAGAATCTGAGAGGAAATAGCCGGGCATCTGcc                                                                                                                                                   >  1:131628/1‑112 (MQ=255)
                                       ggATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCTGGAGAATCTGAGAGGAAATAGCCGGGCATCTGcc                                                                                                                                                   <  2:131628/112‑1 (MQ=255)
                                       ggATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATg                                                                                                                <  3:268991/147‑1 (MQ=255)
                                       ggATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGtt                                                                                                              >  8:107807/1‑149 (MQ=255)
                                       ggATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGt                                                                                                               <  7:25124/148‑1 (MQ=255)
                                         atatTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGtttt                                                                                                            <  7:243610/149‑1 (MQ=255)
                                            ttAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCg                                                                                                         <  2:359009/149‑1 (MQ=255)
                                            ttAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCg                                                                                                         <  1:262743/149‑1 (MQ=255)
                                            ttAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCg                                                                                                         <  6:363802/149‑1 (MQ=255)
                                            ttAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGACCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCg                                                                                                         >  5:322279/1‑149 (MQ=255)
                                              aaaTCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCgg                                                                                                        <  8:32917/148‑1 (MQ=255)
                                               aaTCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCa                                                                                                      <  4:37595/149‑1 (MQ=255)
                                               aaTCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGCAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACAATTCGGTTAAGGTGATGTTTTGCGGCa                                                                                                      >  5:211157/1‑149 (MQ=255)
                                                    tGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGAc                                                                                                   >  4:320065/1‑147 (MQ=255)
                                                    tGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGAc                                                                                                   >  7:201575/1‑147 (MQ=255)
                                                      cATAAAGTCTCCTCTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCTGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAATGTGATGTTTTGCGGCAGACgata                                                                                               <  2:34207/149‑3 (MQ=255)
                                                       aTAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGAt                                                                                             >  6:376790/1‑149 (MQ=255)
                                                         aaCGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGAtt                                                                                            >  5:195508/1‑148 (MQ=255)
                                                          aCGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGAttt                                                                                           <  4:229898/148‑1 (MQ=255)
                                                          aCGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTc                                                                                          <  7:304072/149‑1 (MQ=255)
                                                              ctcCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCa                                                                                      <  5:376790/149‑1 (MQ=255)
                                                               tcCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCag                                                                                     >  8:333959/1‑149 (MQ=255)
                                                                ccTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagc                                                                                    >  1:318615/1‑149 (MQ=255)
                                                                 cTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCgg                                                                                        >  7:94016/1‑144 (MQ=255)
                                                                  tttATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGCGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGCGATTTCGGCagag                                                                                   >  3:303350/1‑146 (MQ=255)
                                                                     ataCAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagc                                                                               >  5:168063/1‑149 (MQ=255)
                                                                      taCAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagcg                                                                              >  3:98679/1‑149 (MQ=255)
                                                                       acaacaGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagcgt                                                                             >  6:17179/1‑149 (MQ=255)
                                                                         aacaGCAGCCTATGATGACGGCTGAAACAGGGTTATGTAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGtt                                                                            >  6:157344/1‑148 (MQ=255)
                                                                            agcagcCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTaaaa                                                                        >  2:87147/1‑149 (MQ=255)
                                                                            agcagcCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTaaa                                                                         <  4:207844/148‑1 (MQ=255)
                                                                            agcagcCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTaaa                                                                         >  3:207844/1‑148 (MQ=255)
                                                                              cagcCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATc                                                                      <  6:224165/149‑1 (MQ=255)
                                                                              cagcCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATc                                                                      <  4:61965/149‑1 (MQ=255)
                                                                                 ccTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTc                                                                   <  2:316903/149‑1 (MQ=255)
                                                                                   tATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCaa                                                                 >  6:222966/1‑149 (MQ=255)
                                                                                   tATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCGGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGGTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCaa                                                                 >  6:5100/1‑149 (MQ=255)
                                                                                   tATGATGACGGCTGAAACAGGGTTATGCAAATAAGGTGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGAAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCTGACGTGCTTTCTGAAGCGTAGCGTTTAACTCTTc                                                                   >  6:115858/1‑147 (MQ=255)
                                                                                     tgatgaCGGCTGAAACAGGGTTATGCAAATCAGGATAATATGAGAGGAAATAGCCGTTCAGATGCCCGTAAAGAGAGAATTAAACTTCGGTTAAGGTTATGTTTTGCGGCAGACGAGATTTCGGCATCGTAGCGTTAAAATCTTAAACg                                                               >  1:93797/1‑149 (MQ=255)
                                                                                      gatgaCGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTc                                                                   <  2:261581/144‑1 (MQ=255)
                                                                                      gatgaCGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCaaga                                                               >  1:261581/1‑146 (MQ=255)
                                                                                          aCGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCtgtg                                                          <  5:301142/149‑1 (MQ=255)
                                                                                          aCGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCtgtg                                                          <  6:97666/149‑1 (MQ=255)
                                                                                           cgccTGAAACAGGGTTATTCAAATCAGGAGAAACTTAGAGGAAAAAGCCGGGCAGATGACCTGCAAGAGAGAATTACACTTCGGTTAATGATATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGtga                                                         <  5:115858/146‑1 (MQ=255)
                                                                                            ggCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGtgat                                                        >  8:235618/1‑149 (MQ=255)
                                                                                             gCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATTGTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGtgatg                                                       <  2:16736/149‑1 (MQ=255)
                                                                                              ccgAAACAGGGCTAAGCAAAACAGGAGAACCTGAGAGCAACTCCCCGGGCAGACCCCCGGCAAGAGACAAAAACACTTCGGATAAGGTGATGCATTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGtgatga                                                      <  8:211797/147‑1 (MQ=255)
                                                                                                  aaCAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGATTTGCGGCAGCCGAGATTTCGGCAGCGTAGCGTTAAAATCTTCTACGCTGTGATGACCtc                                                  >  8:352265/1‑148 (MQ=255)
                                                                                                   aCAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTAc                                                 <  1:87147/149‑1 (MQ=255)
                                                                                                        gtaaTGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGATGTGATGACCTACCGGaa                                            <  1:385978/146‑1 (MQ=255)
                                                                                                         ttATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGaac                                           <  2:318615/149‑1 (MQ=255)
                                                                                                                 aTCACGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTCCACTTCGGTTAAGGTGATGTTTTCCGGCAGACGAGATTTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAaccacc                                    <  3:67966/149‑1 (MQ=255)
                                                                                                                     ggAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACt                               >  1:23052/1‑149 (MQ=255)
                                                                                                                      gatcagcTGAGAGGAAATAGCCGGTCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTg                              <  6:195508/143‑1 (MQ=255)
                                                                                                                      gagaATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTg                              <  7:333959/149‑1 (MQ=255)
                                                                                                                         aaTCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGgtg                           <  5:222966/149‑1 (MQ=255)
                                                                                                                             tGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGc                       >  8:39579/1‑149 (MQ=255)
                                                                                                                               agagGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCt                     >  7:119593/1‑149 (MQ=255)
                                                                                                                                     aaaTAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAACTCTTCTACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTtctc                <  4:98679/149‑1 (MQ=255)
                                                                                                                                      aaTAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGCCGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTatct               >  3:361076/1‑149 (MQ=255)
                                                                                                                                        tAGCCGGGCAGCTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCttt             <  6:281027/149‑1 (MQ=255)
                                                                                                                                        tAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCttt             <  4:142783/149‑1 (MQ=255)
                                                                                                                                         atccGTGCAGATGCCCGGCAAGAGAGAATTACTCTTCTGTTAAGGTGATGTGTTTCGGCTGACGAGATTTCGGAAGCGTAGCGTTAAAATCTTAAACGCTGTGATGTCCTACCGGAACAACAACCAGAATGGTGTAGCCTTTCTCttt             <  6:211157/146‑1 (MQ=255)
                                                                                                                                          gCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCa           <  4:35188/149‑1 (MQ=255)
                                                                                                                                          gCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGGGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTc            <  6:322279/148‑1 (MQ=255)
                                                                                                                                          gCCGGGCAGATGCCCGGCAAGAGACAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATATCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCa           <  8:131874/149‑1 (MQ=255)
                                                                                                                                             gggCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGAc        <  8:303824/149‑1 (MQ=255)
                                                                                                                                                 aGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGACCaaa    <  4:316027/149‑1 (MQ=255)
                                                                                                                                                   aTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGACCAAAtt  <  5:17179/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGTGTGATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGACCAAATT  >  NC_000913/604607‑604901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: