Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 909,587 G→A 7.4% A488V (GCC→GTC)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913909,5870GA 7.4% 161.0 / 3.2 54A488V (GCC→GTC) poxBpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (29/21);  new base A (2/2);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCAT  >  NC_000913/909452‑909732
                                                                                                                                       |                                                                                                                                                 
gCTAACTCTTCTTTGGCGACCACCACATCCACAAACACCGGACAGACGAAGGAGAAGGCGCGTAGCAGGGCGTCTACAACTTCAGACGCTTATTCTACACGGATACCCGTAATGCCGCCCGCGTCGGCAATGCGGGGACAGTTTGTGTc                                                                                                                                      >  7:166337/1‑149 (MQ=255)
 cTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCg                                                                                                                                     <  5:166245/149‑1 (MQ=255)
  ttattattcttTGGCGACGACCACATACATCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGACAAAGttt                                                                                                                                           <  6:246690/136‑1 (MQ=255)
  tAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACAAGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGACAAAGTTtgagatcc                                                                                                                                    >  5:246690/1‑143 (MQ=255)
    aCTCTTCTTTGGCGACAACCACATCCACCAACACCGTCCCGTCGATGGAGAAGGTGAGGTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCAGTATTGCCGCAAGCTTCGGCAATTCGGGGAAAGTTTGGTTCgtg                                                                                                                                   >  6:301052/1‑148 (MQ=255)
      tcttctTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAg                                                                                                                                >  3:48089/1‑149 (MQ=255)
        ttcttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGtt                                                                                                                              >  5:145760/1‑149 (MQ=255)
             tGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCg                                                                                                                            <  1:136651/146‑1 (MQ=255)
                    caccacATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtcag                                                                                                                  >  7:41174/1‑149 (MQ=255)
                          aTCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCaaa                                                                                                             >  1:136098/1‑148 (MQ=255)
                          aTCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCaaa                                                                                                             >  8:101416/1‑148 (MQ=255)
                          aTCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAAt                                                                                                            <  7:295791/149‑1 (MQ=255)
                           tCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCGGTATTGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATa                                                                                                           <  3:308919/149‑1 (MQ=255)
                               ccaACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGcca                                                                                                       <  8:41174/149‑1 (MQ=255)
                               ccaACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGcca                                                                                                       >  3:145510/1‑149 (MQ=255)
                                caACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGccac                                                                                                      <  4:48089/149‑1 (MQ=255)
                                    acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgt                                                                                                                                         <  6:281901/110‑1 (MQ=255)
                                    acCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTtgt                                                                                                                                         >  5:281901/1‑110 (MQ=255)
                                        gACCGTCTATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTc                                                                                              <  3:258284/149‑1 (MQ=255)
                                        gACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTc                                                                                              <  5:301662/149‑1 (MQ=255)
                                          ccGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCAt                                                                                            <  7:101416/149‑1 (MQ=255)
                                           cGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGCTGCCGTCAGTCAAAAATCAACCAGCTTTCAtc                                                                                           >  2:22163/1‑149 (MQ=255)
                                             tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAACTAGCCACCAGCTTTCAtctc                                                                                         >  4:261894/1‑149 (MQ=255)
                                             tCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCAtctc                                                                                         >  3:284961/1‑149 (MQ=255)
                                               gATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCa                                                                                       >  3:363138/1‑149 (MQ=255)
                                                aTGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTATCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTCTCGTGTAGATCGGTGCCGTCAGTCAAAAAGCAACCAGCTTTCATCTCCaa                                                                                      >  6:155503/1‑148 (MQ=255)
                                                 tGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATc                                                                                     >  2:118986/1‑149 (MQ=255)
                                                   gagaAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGc                                                                                   <  1:118986/149‑1 (MQ=255)
                                                       aGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCCCCAGCTTTCATCTCCATCGCcac                                                                                >  5:345643/1‑148 (MQ=255)
                                                          cgcgTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAg                                                                            <  4:145510/149‑1 (MQ=255)
                                                            cgTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGcc                                                                          <  5:155503/149‑1 (MQ=255)
                                                                 cAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGACGTCAGTCAAATAGCCACCAGCTATCATCTCCATCGCCCCAAAGCCCAGCa                                                                     >  6:101258/1‑149 (MQ=255)
                                                                  aGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCAc                                                                    >  7:237689/1‑149 (MQ=255)
                                                                           tccaCTTCCGCCGCTTTTTCTACCCGGATACCCGTAATGCCGCACGCTTCGGCACTGCGGGCAAAGTTTGTGTCGTGTAGGTCGGTGCCGTCAGTCACATAGCCACCCGCTTGCATCTCCATCGCCACACAGCCCAGCACGCtgttgt                                                            >  8:366268/4‑148 (MQ=255)
                                                                             aaCTTCAGACGCTTTTTCTTCACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTa                                                          >  2:99618/1‑148 (MQ=255)
                                                                             aaCTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTa                                                          >  2:257009/1‑148 (MQ=255)
                                                                               cTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGACAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCt                                                                                                 >  6:47573/1‑107 (MQ=255)
                                                                               cTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGACAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCt                                                                                                 <  5:47573/107‑1 (MQ=255)
                                                                                ttCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAga                                                      >  6:218343/1‑149 (MQ=255)
                                                                                  cAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAgac                                                     <  4:363138/148‑1 (MQ=255)
                                                                                       gCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCCCAAAGCCCAGCACGCTGTTCTTAAAGACGACAAt                                               >  5:199312/1‑149 (MQ=255)
                                                                                        cTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtt                                              <  8:237689/149‑1 (MQ=255)
                                                                                             tCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCa                                          <  4:284961/148‑1 (MQ=255)
                                                                                                 cacGGATAACCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGACACAAAGCCCAGCAACCTGTTGTTAAAGACGACAAGTTTCACTgg                                      >  8:112118/1‑148 (MQ=255)
                                                                                                  acGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGCGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCa                                    <  5:192561/149‑1 (MQ=255)
                                                                                                         cccGTAATGCCGCACGCTTCGGCTATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCTGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCACAGCACGCTGTTTTTAAAGACGACAATTTTCACTGGCAGTTTCAt                             <  5:101258/149‑1 (MQ=255)
                                                                                                         cccGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGGGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTGCAt                             <  7:366268/149‑1 (MQ=255)
                                                                                                          ccGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATc                            >  3:111451/1‑149 (MQ=255)
                                                                                                            gTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTg                          <  7:251777/149‑1 (MQ=255)
                                                                                                             tAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGc                         >  3:16525/1‑149 (MQ=255)
                                                                                                               aTGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCac                       <  1:22163/149‑1 (MQ=255)
                                                                                                                 gCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGATCGCTGTCGTCACACTATAATAAAAAAAAATTCATCTCCATCGCCACAAAGCCCAGCACGCTGGTGTTAAAGACGACAATGTTCCCTGGCAGTTTCAGCTGCacta                     >  4:31549/1‑149 (MQ=255)
                                                                                                                          ttCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCCCAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAgg             >  5:90559/1‑148 (MQ=255)
                                                                                                                               cAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCg       >  8:139833/1‑149 (MQ=255)
                                                                                                                                     gggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCAt  >  1:196503/1‑148 (MQ=255)
                                                                                                                                       |                                                                                                                                                 
GCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCAT  >  NC_000913/909452‑909732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑