Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 947,763 C→A 5.6% P179T (CCT→ACT)  ycaM → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913947,7630CA 5.6% 218.3 / 3.5 71P179T (CCT→ACT) ycaMputative transporter
Reads supporting (aligned to +/- strand):  ref base C (33/34);  new base A (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

GCGTTACAGGGGTTAACGCTGGTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAA  >  NC_000913/947616‑947910
                                                                                                                                                   |                                                                                                                                                   
gCGTTACAGGGGTTAACGCTGGTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCgcgc                                                                                                                                                     <  8:248875/148‑1 (MQ=255)
   ttACAGGGGTTAACGCTGGTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGTTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCATGTATGTGGCGATGGCGGTAACCGCGCCTg                                                                                                                                                  >  1:245943/1‑148 (MQ=255)
       aGGGGTTAACGCTGGTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAAtt                                                                                                                                             <  5:16464/149‑1 (MQ=255)
        ggggTTAACGCTGGTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTa                                                                                                                                            >  8:153048/1‑149 (MQ=255)
               aCGCTGGTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGt                                                                                                                                     <  3:37959/149‑1 (MQ=255)
                cGCTGGTTCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTg                                                                                                                                    <  4:93863/149‑1 (MQ=255)
                     gTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCaa                                                                                                                                               <  5:13608/133‑1 (MQ=255)
                     gTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCaa                                                                                                                                               >  6:13608/1‑133 (MQ=255)
                     gTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCat                                                                                                                                 <  1:368073/147‑1 (MQ=255)
                      tGCTGTTTATCTTCTTTATCTGGGTTGCTTCACGCGGTATGAAATCTCAGAAAATCGACGGAACTGTGGCACGCATTCCAATGTTTGTTATCTAACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCAAAtt                                                                                                                              >  6:179206/1‑149 (MQ=255)
                              aTCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACca                                                                                                                       <  4:59239/148‑1 (MQ=255)
                              aTCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACca                                                                                                                       >  3:229727/1‑148 (MQ=255)
                                cttcttTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGATGTTCATATTGCTACcaca                                                                                                                     >  1:298354/1‑148 (MQ=255)
                                 ttcgtTATGTGGGTTGCTTCACGCGGAATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATAATGCGAACACaac                                                                                                                   >  6:293549/1‑148 (MQ=255)
                                       aTGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATAGTCGGTTCTGTGGCAGGGATTGCTATGGTTGTTATGTGACTCCTGTATGTGTCGATGAAGGTAACCGCGCCTGCATTTACTGAAGTTCATATTGAGACCACAACCATTAc                                                                                                             >  2:271665/1‑149 (MQ=255)
                                        tgtcGGTTGCTTCACGCGGTATGAAATCGCTGAAACTCGTCGGTTCTGTGGCAGGGCTTGCTATGTTTGTTATGGCACTCCTGTATGTGGCGATGTCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGTCCTCTAACAATAcc                                                                                                            <  8:167778/149‑1 (MQ=255)
                                         gtgGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTAcc                                                                                                            <  4:229727/148‑1 (MQ=255)
                                            ggTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTgg                                                                                                         >  3:272149/1‑148 (MQ=255)
                                               tGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGCCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGaaa                                                                                                     >  7:145106/1‑149 (MQ=255)
                                                  ttCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCGATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGGATATTGCGACCACAAACATTACCTGGGAAACGt                                                                                                  >  4:72449/1‑149 (MQ=255)
                                                       gcgGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCAtt                                                                                             >  8:176680/1‑149 (MQ=255)
                                                        cgGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTc                                                                                            >  6:228124/1‑149 (MQ=255)
                                                         ggTATTAAATCGCTGAAAATCGTCGGTTCTGTCGCAGGGATTGATATGTTTGTTATGTCACTCCTGTATGTGTCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTcc                                                                                           <  5:244681/149‑1 (MQ=255)
                                                           tATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGATACGTTCATTCCTc                                                                                         <  8:103792/149‑1 (MQ=255)
                                                           tATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTc                                                                                         <  1:225398/149‑1 (MQ=255)
                                                           tATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGATATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTc                                                                                         <  6:227667/149‑1 (MQ=255)
                                                             tGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCat                                                                                       <  7:40017/149‑1 (MQ=255)
                                                                aaTCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGACTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCAt                                                                                              >  4:306286/1‑139 (MQ=255)
                                                                aaTCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGACTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCAt                                                                                              >  4:301134/1‑139 (MQ=255)
                                                                aaTCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGACTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCAt                                                                                              <  3:301134/139‑1 (MQ=255)
                                                                aaTCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGACTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCAt                                                                                              <  3:306286/139‑1 (MQ=255)
                                                                    gCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGCCCACAAACATTACCTGGGCAACGTTCATGCCTCATATCGACt                                                                                >  4:251076/1‑149 (MQ=255)
                                                                        aaaaTCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACt                                                                                                                                          >  1:191224/1‑87 (MQ=255)
                                                                        aaaaTCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACt                                                                                                                                          <  2:191224/87‑1 (MQ=255)
                                                                         aaaTCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTAcc                                                                           <  3:92217/149‑1 (MQ=255)
                                                                          aaTCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCt                                                                          <  7:193314/149‑1 (MQ=255)
                                                                               tcgGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACAt                                                                      >  8:62051/1‑148 (MQ=255)
                                                                               tcgGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACAt                                                                      >  8:309195/1‑148 (MQ=255)
                                                                                  gTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTc                                                                                         <  7:1584/126‑1 (MQ=255)
                                                                                  gTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTc                                                                                         >  8:1584/1‑126 (MQ=255)
                                                                                  gTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTAcc                                                                  <  8:32960/149‑1 (MQ=255)
                                                                                        tgGCAGGGATTGCTATGTTTGTTATGGCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTa                                                              <  2:122842/147‑1 (MQ=255)
                                                                                              ggATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTACCCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATg                                                      >  7:359100/1‑149 (MQ=255)
                                                                                                     tATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTGACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGttt                                               >  3:167399/1‑149 (MQ=255)
                                                                                                       tttttgttATGTCACTCCTGTATGTGTCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGAATATTGCGACCACAAACATTACATGTGAAACGTTCATTCCTGATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTc                                             <  5:179206/147‑1 (MQ=255)
                                                                                                        gtttgttATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATTTTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTcg                                            >  8:321489/1‑149 (MQ=255)
                                                                                                         tttgttATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCata                                                                                      >  5:33817/1‑106 (MQ=255)
                                                                                                         tttgttATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCata                                                                                      <  6:33817/106‑1 (MQ=255)
                                                                                                              tATGTCACTCCTGTATGTGGCGATGGCTGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTg                                                   <  5:120973/136‑1 (MQ=255)
                                                                                                              tATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTg                                                   >  6:120973/1‑136 (MQ=255)
                                                                                                                tGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTgg                                     <  7:176680/148‑1 (MQ=255)
                                                                                                                tGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGGTGGc                                    <  3:41978/149‑1 (MQ=255)
                                                                                                                 gTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCg                                   >  5:373283/1‑149 (MQ=255)
                                                                                                                 gTCACTCCTGTATCTGGCGATGGCGGTAACCGCGCCTGCCATTACTGAAGTGCATATTGCGACCACAAACAATAGCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACAGTACCACTATTTCAATGCTCGATGTCGCGGTTGGCg                                   >  4:356894/1‑149 (MQ=255)
                                                                                                                  tCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTAc                                                                            <  5:176893/107‑1 (MQ=255)
                                                                                                                  tCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTAc                                                                            >  6:176893/1‑107 (MQ=255)
                                                                                                                  tCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCg                                   <  7:62051/148‑1 (MQ=255)
                                                                                                                   cACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCg                                   >  4:270969/1‑147 (MQ=255)
                                                                                                                    aCTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAg                                >  2:371265/1‑149 (MQ=255)
                                                                                                                     cTCCTGTATGTGGCGATGTCGGTAACAGCGCCTGAAATTACTGAAGTGCATATTGCGACAAAAAACATTACCTGGGAAAGGTGCATTCAGAAATAGGAATTAACAGAAATTCCCAATATTTCAATAATGGTTTTCGAGGTTGGCGGAgg                               >  6:89641/1‑148 (MQ=255)
                                                                                                                      tCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAg                                >  5:128299/1‑147 (MQ=255)
                                                                                                                         tGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCa                              >  8:222724/1‑146 (MQ=255)
                                                                                                                           tATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTgg                                     >  8:269722/1‑137 (MQ=255)
                                                                                                                           tATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTgg                                     <  7:269722/137‑1 (MQ=255)
                                                                                                                           tATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGagaa                         <  3:270969/149‑1 (MQ=255)
                                                                                                                              gtgGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTAc                                                                            <  6:264598/95‑1 (MQ=255)
                                                                                                                              gtgGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTAc                                                                            >  5:264598/1‑95 (MQ=255)
                                                                                                                                 gCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTtc                   <  5:228124/149‑1 (MQ=255)
                                                                                                                                   gATTGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACAACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTtctc                 <  2:245943/149‑1 (MQ=255)
                                                                                                                                   gATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTtctc                 <  7:153048/149‑1 (MQ=255)
                                                                                                                                     tGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCt               <  4:272149/149‑1 (MQ=255)
                                                                                                                                          gTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGt          <  6:373283/149‑1 (MQ=255)
                                                                                                                                                gcgcCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATc     >  8:239472/1‑148 (MQ=255)
                                                                                                                                                  gcCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCaaa  <  8:145106/149‑1 (MQ=255)
                                                                                                                                                  gcCTGCAATAACTGAAGTGCATATTGCGACCACAAACATTACCTCGGAAACGTTCATACCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCaaa  <  2:87610/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGTTACAGGGGTTAACGCTGGTGCTGTTTATCTTCTTTATGTGGGTTGCTTCACGCGGTATGAAATCGCTGAAAATCGTCGGTTCTGTGGCAGGGATTGCTATGTTTGTTATGTCACTCCTGTATGTGGCGATGGCGGTAACCGCGCCTGCAATTACTGAAGTGCATATTGCGACCACAAACATTACCTGGGAAACGTTCATTCCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAA  >  NC_000913/947616‑947910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: