Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,303,096 C→G 6.8% Y66* (TAC→TAG oppB → oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,303,0960CG 6.8% 282.0 / 8.7 85Y66* (TAC→TAGoppBoligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (40/39);  new base G (2/4);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTG  >  NC_000913/1302949‑1303244
                                                                                                                                                   |                                                                                                                                                    
tattCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAGGCCCTTTTACCAGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTAc                                                                                                                                                      <  6:335255/148‑1 (MQ=255)
tattCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTAc                                                                                                                                                      <  4:217371/148‑1 (MQ=255)
  ttCTTATTACTTTTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCt                                                                                                                                                    <  3:310436/148‑1 (MQ=255)
     tttttaCTTTTTGGTTCTTTTTGATGCGCGTCGCGCGGGGAAGCCGTTTTAGCGGCGAACGTACTTTACCGCCAGAAGTGTTGTCGTATATCGTAGCGTTATTTCATGTTAATGATCCGATCTTGTCACAGTATTTCTGCTAGCTGaaa                                                                                                                                                <  6:206773/146‑1 (MQ=255)
     ttattaCTATTTCGTTCTTTTTGATGCGCCTCGCGTCGGGAAGCCCTTTTAGCGGCGAACGTACTTTACCGCCAGAAGTGATGGCGAATATCGAAGCGATTTATCATCTTAATGATCCTATCTTGTCACAGTATTTCAGCTAGCTGaaa                                                                                                                                                <  6:212121/149‑1 (MQ=255)
         taCTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAaacaac                                                                                                                                            >  7:408530/1‑149 (MQ=255)
            tATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAATGTACTTTACAGCCATAAGTGATGGCCAATATCGAAGAGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTgg                                                                                                                                         >  3:21122/1‑149 (MQ=255)
              tttCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCTAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTgg                                                                                                                                         <  3:5881/147‑1 (MQ=255)
              tttCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGgc                                                                                                                                        <  4:272763/148‑1 (MQ=255)
              tttCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGgc                                                                                                                                        >  3:272763/1‑148 (MQ=255)
              tttCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGAAACAGTATTTCCGATACATGAAACAACTGgcg                                                                                                                                       >  6:31503/1‑149 (MQ=255)
                   ttCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGGTAGCTGaaatactgt                                                                                                                                          <  7:303725/141‑7 (MQ=255)
                   ttCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGGTAGCTGaaatactgt                                                                                                                                          <  7:110233/141‑7 (MQ=255)
                   ttCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGGTAGCTGaaatactgt                                                                                                                                          >  8:110233/1‑135 (MQ=255)
                   ttCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGGTAGCTGaaatactgt                                                                                                                                          >  8:303725/1‑135 (MQ=255)
                        tATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGAtt                                                                                                                             >  3:301554/1‑149 (MQ=255)
                          tgatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCGAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTc                                                                                                                           >  5:185947/1‑149 (MQ=255)
                          tgatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGAttt                                                                                                                            <  1:17994/148‑1 (MQ=255)
                          tgatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGAttt                                                                                                                            <  3:294847/148‑1 (MQ=255)
                           gatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCt                                                                                                                                                    >  7:16315/1‑123 (MQ=255)
                           gatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCt                                                                                                                                                    <  8:16315/123‑1 (MQ=255)
                           gatgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCg                                                                                                                          >  5:131521/1‑149 (MQ=255)
                            atgCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGACAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCg                                                                                                                          <  5:234889/148‑1 (MQ=255)
                             tgCGCCTCGCGCCGGGAAGCCCGTTTACCGGCGAACTTACTTTACCGCCAGAAGTGATGGCAAATATAGAAGCGAAATATCATATTAATTATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTTATTTCGGt                                                                                                                        >  6:138077/1‑149 (MQ=255)
                                 ccTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCAt                                                                                                                    >  7:120903/1‑149 (MQ=255)
                                  cTCGCGCCGGGAAGCCCTTTTACCTGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATc                                                                                                                   >  3:320257/1‑149 (MQ=255)
                                  cTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATc                                                                                                                   >  3:325534/1‑149 (MQ=255)
                                  cTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATc                                                                                                                   >  3:184007/1‑149 (MQ=255)
                                  cTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATc                                                                                                                   <  8:408530/149‑1 (MQ=255)
                                     gcgcCGGGAAGCCGTTTTACCGGCGACCGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGATATATCAGCTTAATGATACGATCATGACACAGTATTTCCGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCAATCGtt                                                                                                                >  5:125305/1‑149 (MQ=255)
                                               gCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATaaa                                                                                                      <  7:383401/149‑1 (MQ=255)
                                                 ccTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAAtata                                                                                                        >  4:82735/1‑145 (MQ=255)
                                                 ccTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATAGCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAAtata                                                                                                        >  7:386782/1‑145 (MQ=255)
                                                        ccGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCg                                                                                             >  4:24664/1‑149 (MQ=255)
                                                         cGGCGAACGTACTTTACCGCCAGAAGTGATTGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCgg                                                                                            <  1:137561/149‑1 (MQ=255)
                                                              aaCGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAAt                                                                                       <  5:69911/149‑1 (MQ=255)
                                                               aCGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAAt                                                                                       >  5:304227/1‑148 (MQ=255)
                                                                 gTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGAGTTCGCTCCATCGTTTAAATATAAAGATTATTCGGTCAATGAc                                                                                    >  2:258238/1‑149 (MQ=255)
                                                                   aCTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATAACATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACtt                                                                                  >  7:319304/1‑149 (MQ=255)
                                                                     tttACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCATAATGATCCGATCATGCCACAGTATTTCAGCTACCTGAGACAACTGGCGCACGGTTATTTCGGTCCATCGATTAAATATAAAGATTATTAGGTCAATGCGTTgg                                                                                >  6:243714/1‑149 (MQ=255)
                                                                      ttACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTaa                                                                                                             >  2:316897/1‑119 (MQ=255)
                                                                      ttACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTaa                                                                                                             <  1:316897/119‑1 (MQ=255)
                                                                        aCCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCTATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTgggt                                                                              <  5:59236/148‑3 (MQ=255)
                                                                        aCCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGtt                                                                              <  5:300746/148‑1 (MQ=255)
                                                                         ccGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGc                                                                            <  8:120903/149‑1 (MQ=255)
                                                                           gCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCAt                                                                          >  4:167534/1‑149 (MQ=255)
                                                                           gCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCAt                                                                          <  3:82735/149‑1 (MQ=255)
                                                                            ccAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATc                                                                         <  5:364979/149‑1 (MQ=255)
                                                                            ccAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAAAGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGAAGTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATc                                                                         <  6:82941/149‑1 (MQ=255)
                                                                              aGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCa                                                                       <  3:24664/149‑1 (MQ=255)
                                                                                     aTGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTcc                                                                >  5:328138/1‑149 (MQ=255)
                                                                                     aTGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTcc                                                                >  7:23134/1‑149 (MQ=255)
                                                                                         ccAATATCGAAGCGAAATATCATCTTAATGCTCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGtt                                                            >  6:154696/1‑149 (MQ=255)
                                                                                         ccAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGtt                                                            >  7:364970/1‑149 (MQ=255)
                                                                                          cAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGttt                                                           <  1:91917/149‑1 (MQ=255)
                                                                                           aaTATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTc                                                          <  4:325534/149‑1 (MQ=255)
                                                                                           aaTATATAATCGAAATATCATCTTAATTATCCGATCATGACACAGTATTTCAGCTACCTGAAAAAACTGGAGCACGGTGATTTAGGTCAATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGttta                                                          <  4:320257/149‑2 (MQ=255)
                                                                                               tCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGc                                                       >  8:365977/1‑148 (MQ=255)
                                                                                               tCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGc                                                       >  8:57886/1‑148 (MQ=255)
                                                                                                 gAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCa                                                     >  2:177574/1‑148 (MQ=255)
                                                                                                     cGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTg                                                >  1:332996/1‑149 (MQ=255)
                                                                                                      gAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTc                                                          >  3:320083/1‑138 (MQ=255)
                                                                                                      gAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTc                                                          <  4:320083/138‑1 (MQ=255)
                                                                                                       aaaTATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGGGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTggg                                              <  6:185947/149‑1 (MQ=255)
                                                                                                              atcTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCgc                                        <  8:386782/148‑1 (MQ=255)
                                                                                                              atcTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCg                                         >  4:267058/1‑147 (MQ=255)
                                                                                                                 ttAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCgcagca                                    >  3:217480/1‑149 (MQ=255)
                                                                                                                   aaTGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCAtt                                  >  6:376732/1‑149 (MQ=255)
                                                                                                                    aTGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCAttt                                 >  3:248076/1‑149 (MQ=255)
                                                                                                                    aTGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCAttt                                 <  3:167534/149‑1 (MQ=255)
                                                                                                                    aTGATACTATCATGACACAGTATTTCAGCTTCCTGAAAAAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCAtt                                  <  5:31503/148‑1 (MQ=255)
                                                                                                                     tGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATTACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCAtttt                                <  3:93326/149‑1 (MQ=255)
                                                                                                                      gATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCAttttt                               >  2:237717/1‑149 (MQ=255)
                                                                                                                            aTCCTGACACAGGATTTCAGCGACCTGAAACAACTGGCGCACGGGGATTTCGGGCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTGGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGc                         <  6:125305/149‑1 (MQ=255)
                                                                                                                            aTCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGc                         <  3:267058/149‑1 (MQ=255)
                                                                                                                             tCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCg                        <  1:237717/149‑1 (MQ=255)
                                                                                                                               aTGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCgg                       <  8:319304/148‑1 (MQ=255)
                                                                                                                               aTGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCgg                       <  8:23134/148‑1 (MQ=255)
                                                                                                                                  acacAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTaa                    <  6:131521/148‑1 (MQ=255)
                                                                                                                                   cacaGTATTTCAGCTACCTGAAACAACTGGAGCACGTTGATTTCGGTCCATCGTTTAAATATAACGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCATACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATa                  <  5:243714/149‑1 (MQ=255)
                                                                                                                                    acaGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATaa                                                                                                       <  8:224075/63‑1 (MQ=255)
                                                                                                                                    acaGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATaa                                                                                                       >  7:224075/1‑63 (MQ=255)
                                                                                                                                      aGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTg               >  6:183289/1‑149 (MQ=255)
                                                                                                                                      aGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGAGTATTCGGTCAAGGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCCTTTTTCCTTGCGGTAATACTg               >  3:278446/1‑149 (MQ=255)
                                                                                                                                       gTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGGCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTgg              <  7:167356/149‑1 (MQ=255)
                                                                                                                                            tCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGgtgt          <  1:177574/148‑1 (MQ=255)
                                                                                                                                               gCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGt      <  6:304227/149‑1 (MQ=255)
                                                                                                                                                   ccTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTg  >  1:38310/1‑149 (MQ=255)
                                                                                                                                                   ccTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTg  <  7:365977/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTG  >  NC_000913/1302949‑1303244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: