| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,466,336 | C→G | 7.5% | pseudogene (945/2513 nt) | ydbA → | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,466,336 | 0 | C | G | 7.5% | 167.3 / 3.7 | 52 | pseudogene (945/2513 nt) | ydbA | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
| Reads supporting (aligned to +/- strand): ref base C (27/21); new base G (2/2); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATT > NC_000913/1466198‑1466483 | cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg < 1:21050/149‑1 (MQ=255) ggTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATATGGAGTCCATGTGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATgg < 2:109357/149‑1 (MQ=255) gTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGt < 5:52997/149‑1 (MQ=255) tGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtg < 2:35335/149‑1 (MQ=255) aCAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgat < 8:225887/149‑1 (MQ=255) aGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTACATGGGTATCCAGATCGACGGTGACAAGGCCAACGTCAATAACGAAGGCGAGCGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgattc > 3:334035/1‑147 (MQ=255) gATTACAAGGTCAATATGAACGTAATCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGAAAAGGACATCGTCAATAACGATTGCGAGAGCACCATCACCTACGGTGGCACCGGCACCCAGTTTAATGGTGATGACGCCACGGCaaa < 8:148730/149‑1 (MQ=18) tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGGACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaaca < 6:127146/149‑1 (MQ=255) tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGCTGACGCCACGGCAaac > 3:40176/1‑148 (MQ=255) tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaac > 1:311559/1‑148 (MQ=255) aaCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCCCCGGTGGCACCGGCACCCCGATTAATGGTGATGACGCCCCGGCAaacaa > 8:379205/1‑149 (MQ=255) aGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaacaac < 8:319012/149‑1 (MQ=255) gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCATACAACAACGGc < 6:140604/149‑1 (MQ=255) cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGCCAAGGCCATCGTCAATAACGAAGGCGAGAGCCCCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCaaaa > 5:56332/1‑149 (MQ=255) cGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCg > 7:333902/1‑149 (MQ=255) cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg < 7:401213/149‑1 (MQ=255) cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGACAAAAACGAAGGCGAGAGCCCCATCACCACCGGTGGCACCGGCACCCACATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg > 1:165950/1‑149 (MQ=17) aTCCGGAGTCCATGGGTATCCAGATCGCCGGTGACAAGGCCATCGTCAATAACGAAGGCGCGAGCACAATCACCAACGGTGGCCACGCCACCCACATTAATGGTGATGACGCCCAGCAAAACAACAACGGCAAACACACCGtgcacgt > 2:313285/1‑142 (MQ=18) cGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa < 2:311559/148‑1 (MQ=255) gAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGtt < 6:234845/138‑1 (MQ=255) gAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGtt > 5:234845/1‑138 (MQ=255) cATGGGTATCCAGATTGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCa > 1:168239/1‑149 (MQ=255) aTGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc > 7:98381/1‑149 (MQ=255) gTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAACACCACCGTTGACGGCAAGGCTTCCACCgg > 1:384315/1‑148 (MQ=255) tATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaac < 6:96723/109‑1 (MQ=255) tATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaac > 5:96723/1‑109 (MQ=255) ccAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACg > 3:335157/1‑148 (MQ=255) aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCCCCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAAc > 5:378934/1‑149 (MQ=255) aCAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCCAACAACAACGGCAACACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGtaa > 5:276736/1‑148 (MQ=255) aCAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGtaa > 1:129095/1‑148 (MQ=255) cATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGa > 7:203911/1‑148 (MQ=255) tCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGaaaa < 5:287750/149‑1 (MQ=255) aaTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTa < 2:129095/148‑1 (MQ=255) aaTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTa < 2:168239/148‑1 (MQ=255) tAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg < 4:34392/98‑1 (MQ=9) tAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAAGGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg > 3:34392/1‑98 (MQ=9) tAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCTGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGa < 5:262234/96‑1 (MQ=255) tAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCGGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGa > 6:262234/1‑96 (MQ=255) tAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTAtt > 5:294717/1‑148 (MQ=255) tAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTc < 1:11870/149‑1 (MQ=255) gAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACCACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGa > 1:52074/1‑149 (MQ=255) aGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACg > 7:255849/1‑149 (MQ=255) ggCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACgg > 5:82855/1‑122 (MQ=255) ggCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACgg < 6:82855/122‑1 (MQ=255) ggCGAGAGCACCATCACCAACGGTGGCAACGGCACCCAGATTAATGGTGATGACGACACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACgg < 2:366044/149‑1 (MQ=255) gCACCATCACCAACGGGGGCACCGGCACCCAGATTACTGGTGATGACGACACGGAAAACAACAACGGCAAAACCAACGTTGCCGGCAAGGATTCCACCGGTACGGTAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTg < 1:313285/149‑1 (MQ=17) cATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAAAAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATg > 8:72528/1‑149 (MQ=255) aCCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCa < 8:98381/148‑1 (MQ=255) aCCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAg > 7:257996/1‑149 (MQ=255) cAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcg > 8:142891/1‑149 (MQ=255) cAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcg > 2:106158/1‑149 (MQ=255) aaCGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACACCGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAAAGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcgg > 1:150882/1‑149 (MQ=255) ccggcaccCAGATTTATGTTCATGACGCCACGGAAAACAACAACGGCAAATCCACCTTTGTCGGCAAGGATTCCACCGTTACGGAATTCAACGGTAATAACGGAAAAGTTATTCAGGACGTCGATCTGGATGTCATCGGCGGCGGTCAc < 4:334035/149‑1 (MQ=255) cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg > 7:320309/1‑149 (MQ=255) cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg < 4:40176/149‑1 (MQ=255) gcaccCAGATTAATGGTTATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTTACGCCAAGGATTCCACCTCTACGGAAATCAACGGTAATAAAGGAAAAGGAATTAAGGAATACGAACTGGATGTCAGAGGCCGCGGTCAcag > 2:303092/1‑146 (MQ=17) accCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt < 8:333902/149‑1 (MQ=255) cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGt < 8:118810/89‑1 (MQ=255) cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGt > 7:118810/1‑89 (MQ=255) ccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATAAAAGGAAATAACGGAAAAGTTATTCAGTACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt > 4:160322/1‑148 (MQ=255) | CGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATT > NC_000913/1466198‑1466483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |