Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,770,800 T→C 5.0% L62L (CTT→CTC ydiL → putative HTH domain DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,770,8000TC 5.0% 247.5 / 2.8 79L62L (CTT→CTCydiLputative HTH domain DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (42/33);  new base C (2/2);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

ACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCA  >  NC_000913/1770660‑1770947
                                                                                                                                            |                                                                                                                                                   
aCTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCAGTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCcat                                                                                                                                             >  2:100091/1‑149 (MQ=255)
 cTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCcat                                                                                                                                             >  6:266274/1‑148 (MQ=255)
    ttGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCcatca                                                                                                                                           >  1:398936/1‑147 (MQ=255)
      gatgaATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATa                                                                                                                                         >  7:146953/1‑147 (MQ=255)
         gaATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAgaga                                                                                                                                     >  1:182660/1‑148 (MQ=255)
              cgcgACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAaaataa                                                                                                                               <  7:125236/149‑1 (MQ=255)
              cgcgACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAaaataa                                                                                                                               <  1:2939/149‑1 (MQ=255)
              cgcgACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAaaata                                                                                                                                <  2:238590/148‑1 (MQ=255)
                 gACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAaataaata                                                                                                                            <  2:182660/149‑1 (MQ=255)
                            cccAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCtt                                                                                                                                                     <  3:295576/113‑1 (MQ=255)
                            cccAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCtt                                                                                                                                                     >  4:295576/1‑113 (MQ=255)
                            cccAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCgg                                                                                                                  <  4:334096/148‑1 (MQ=255)
                            cccAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCgg                                                                                                                  >  7:145830/1‑148 (MQ=255)
                             ccAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATTCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGAATCGGc                                                                                                                 >  2:104391/1‑148 (MQ=255)
                             ccAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGc                                                                                                                 >  8:332726/1‑148 (MQ=255)
                             ccAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGc                                                                                                                 >  8:379448/1‑148 (MQ=255)
                              cataCCGGTGACAGTGAAAGCTGGCGTCAGTTGGAAAATTGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGATGGCTAAGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGcaa                                                                                                               <  1:388331/146‑1 (MQ=255)
                              cAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGcaa                                                                                                               <  1:382990/149‑1 (MQ=255)
                                  ccGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGcaacaa                                                                                                            <  2:398936/148‑1 (MQ=255)
                                  ccGGTGACAGTGAAAGCTGGAGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGcaacaa                                                                                                            >  4:209255/1‑148 (MQ=255)
                                     gTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAacac                                                                                                         >  2:186293/1‑148 (MQ=255)
                                      tGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCa                                                                                                       >  1:58523/1‑149 (MQ=255)
                                        aCAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATg                                                                                                     >  7:132039/1‑149 (MQ=255)
                                          aGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCg                                                                                                   >  5:152195/1‑149 (MQ=255)
                                          aGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCg                                                                                                   >  3:76523/1‑149 (MQ=255)
                                           gTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGt                                                                                                  >  2:191275/1‑149 (MQ=255)
                                           gTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGt                                                                                                  <  1:239166/149‑1 (MQ=255)
                                            tGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGtt                                                                                                 >  1:127189/1‑149 (MQ=255)
                                               aaGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGttttt                                                                                              >  2:108149/1‑149 (MQ=255)
                                               aaGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGttttt                                                                                              >  3:77980/1‑149 (MQ=255)
                                                 gCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAATAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTc                                                                                            <  4:223401/149‑1 (MQ=255)
                                                  atGGCGTCAGTGGGATAATGGCAAGTGTGCCATTCCTGATCGTTTAGTCGAGCAACTGTTGGCTATGCTTCAGCAAAGAAAAAAACATCTTCATGCCAGCTTAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTcc                                                                                           <  2:250959/148‑1 (MQ=255)
                                                     gCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTcccc                                                                                         <  2:58523/148‑1 (MQ=255)
                                                      cGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTTGCTATGCGTCAGCAAAGAAAAAACCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGa                                                                                       <  1:297957/149‑1 (MQ=255)
                                                       gTCAGTGGGAAAGTGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGAc                                                                                      >  6:104965/1‑149 (MQ=255)
                                                       gTCAGTGGGAAAATGGCACGTGTGCCATTCCTGCTCGTGGAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAACACATCTTCATGCCATCATAGAGAAAATAAATAACCGCAGCGGCAACAACACCATGCGTTTTTTCCCCGAc                                                                                      >  3:302988/1‑149 (MQ=255)
                                                        tCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATg                                                                                                                                                 >  4:84586/1‑89 (MQ=255)
                                                        tCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATg                                                                                                                                                 <  3:84586/89‑1 (MQ=255)
                                                         cAGTGGGAAAATGGCAAGTGTACCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACt                                                                                     >  1:152340/1‑148 (MQ=255)
                                                            tGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTaa                                                                                  <  3:323308/148‑1 (MQ=255)
                                                            tGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTaa                                                                                  >  4:323308/1‑148 (MQ=255)
                                                            tGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAAc                                                                                 <  1:191275/149‑1 (MQ=255)
                                                            tGGGAAAATGGCAAGTGGGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCTCATCGGCAACAACACCCTGCGTTTTTTCCCCGACTTAAc                                                                                 <  2:345918/149‑1 (MQ=255)
                                                              ggAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCACCTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACCACACCATGAGGTTGTTCCCCGCCTTACCCg                                                                               >  6:247019/1‑149 (MQ=255)
                                                              ggAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAAcc                                                                                <  1:186293/148‑1 (MQ=255)
                                                                aaaaTGGCAAGGGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCa                                                                             <  5:247019/149‑1 (MQ=255)
                                                                      gCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCaa                                                                       <  7:332726/149‑1 (MQ=255)
                                                                           tgtgCCATTCCTGATCGTGTAGTCGATCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGt                                                                  <  1:100091/149‑1 (MQ=255)
                                                                               ccATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTAc                                                              >  3:365575/1‑149 (MQ=255)
                                                                                 aTTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTAccc                                                            <  7:379448/149‑1 (MQ=255)
                                                                                    ccTGATCGTGTAGTCGCGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACACCACCATGCGTTGTTTCCCCGCCTTATCCGCCTTTCAACGAGTCTACCCt                                                           >  2:354227/1‑147 (MQ=255)
                                                                                     cTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGAt                                                        >  2:375685/1‑149 (MQ=255)
                                                                                      tGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATg                                                       >  4:128145/1‑149 (MQ=255)
                                                                                         tCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGc                                                     >  3:210054/1‑148 (MQ=255)
                                                                                            tgtAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAAtt                                                 >  1:229042/1‑149 (MQ=255)
                                                                                            tgtAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAAtt                                                 <  8:146953/149‑1 (MQ=255)
                                                                                             gtAGTCGAGCAACTGTTGTCTATGCGTCAGAAAAGAAAAAAACATCTTCATGCAATCATAGAGAAAATAAATAACCGCATCGGAAACAACACCATGCGTATTTTACCAGACTTAACCGCATTTCAACGAGTCTACCCTTATCGCAAttt                                                >  5:109349/1‑149 (MQ=255)
                                                                                              tAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAAtttt                                               <  5:266274/149‑1 (MQ=255)
                                                                                                gTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTa                                              <  8:132039/148‑1 (MQ=255)
                                                                                                gTCGAGCAACTGTTGGCTATGCGTCACCAAAGAAAAAAACATCTTCATGCAATCATAGAGAAAATAAATAACCGCATCGGCATCCACACCATGCGTTTTTTCCCCGGCTTAACCGAATTTCAACGAGTCGACCCTGATGGCAATTTTAt                                             >  8:117641/1‑149 (MQ=255)
                                                                                                  cGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTAtt                                            <  3:209255/148‑1 (MQ=255)
                                                                                                     gCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACt                                        <  6:152195/149‑1 (MQ=255)
                                                                                                        aCTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTgg                                      >  1:29011/1‑148 (MQ=255)
                                                                                                         cTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTCCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTAc                                                              >  8:265251/1‑123 (MQ=255)
                                                                                                         cTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTCCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTAc                                                              <  7:265251/123‑1 (MQ=255)
                                                                                                         cTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTCCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTAc                                                              <  7:271520/123‑1 (MQ=255)
                                                                                                         cTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTCCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTAc                                                              >  8:271520/1‑123 (MQ=255)
                                                                                                            ttGGCTATGCGTCAGCAAAGAAAAACAAATCTTCATTCAATCATAGAGAAAATAAATAACCTCATCGGCAACAACACCATGCGTTATTTCCCCGACTTAAACGAATTAAAACTATTATACCCTCAAGTCAATTTTATTTACTGTAAGa                                  >  8:205657/1‑148 (MQ=255)
                                                                                                            ttGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGAt                                 <  4:365575/149‑1 (MQ=255)
                                                                                                            ttGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGAAAAAACACCATGCGGGTTTTCCCCGACTTAACCGCATTTCAACGCGTCTACCCTGCTGGCAATTTTATTGACTGGAatat                                 >  6:63460/1‑149 (MQ=255)
                                                                                                                cTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTAt                             <  5:104965/149‑1 (MQ=255)
                                                                                                                    gCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAAt                         >  5:157867/1‑149 (MQ=255)
                                                                                                                         aGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCgg                      >  6:87306/1‑147 (MQ=255)
                                                                                                                           cAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATACCCGCATCGGCAACAACACCATGCGTTTTTTCCCCTACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTATATTGACTGGAAGATATATCAATCGGTAgc                  >  1:141249/1‑149 (MQ=255)
                                                                                                                           cAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAgc                  >  1:198844/1‑149 (MQ=255)
                                                                                                                                 aaaaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGa            <  2:29011/149‑1 (MQ=255)
                                                                                                                                  aaaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGaa           >  7:4189/1‑149 (MQ=255)
                                                                                                                                   aaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATTGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAAt          <  4:77980/149‑1 (MQ=255)
                                                                                                                                    aaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAAt          >  8:293413/1‑148 (MQ=255)
                                                                                                                                     aaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATta        <  5:87306/149‑1 (MQ=255)
                                                                                                                                       cATATTCCTGCCATCATAGAGAAACTAAATAACCGCTTCTGCACCTACACCATTCTTTTTTTCCCCGACTTAACCTCATTTCAACTAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATtata      <  4:257717/149‑1 (MQ=255)
                                                                                                                                            tCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACCCCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGca  >  6:139612/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
ACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCA  >  NC_000913/1770660‑1770947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: