Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,854,790 A→T 6.0% V22D (GTC→GAC)  ydjF ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,854,7900AT 6.0% 219.5 / 3.3 76V22D (GTC→GAC) ydjFputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (36/35);  new base T (2/3);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAAT  >  NC_000913/1854642‑1854937
                                                                                                                                                    |                                                                                                                                                   
cTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTgaga                                                                                                                                                     <  3:293839/149‑1 (MQ=255)
   ataAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTg                                                                                                                                                  <  4:27928/148‑1 (MQ=255)
   ataAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCAACATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTg                                                                                                                                                  >  2:158366/1‑149 (MQ=255)
    taAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGt                                                                                                                                                 >  5:30687/1‑149 (MQ=255)
    taAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTAAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGt                                                                                                                                                 >  5:25280/1‑149 (MQ=255)
         aTGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCt                                                                                                                                            <  1:59859/149‑1 (MQ=255)
            gATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCtttt                                                                                                                                         <  2:74253/149‑1 (MQ=255)
            gATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCtttt                                                                                                                                         >  8:110557/1‑149 (MQ=255)
            gATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCtttt                                                                                                                                         <  7:110557/149‑1 (MQ=255)
                     cGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGtt                                                                                                                                  <  8:343539/147‑1 (MQ=255)
                     cGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTg                                                                                                                                 <  3:12547/148‑1 (MQ=255)
                             tCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCAtt                                                                                                                        >  7:339706/1‑149 (MQ=255)
                                cTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGc                                                                                                                     <  8:40899/149‑1 (MQ=255)
                                 tGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCGCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCt                                                                                                                    <  6:30687/149‑1 (MQ=255)
                                  gTATTTAAAACAGCACCACCATAGGTTCTGCTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCAATCAAATTTTAGACTGTAACCTTTTTATCGTTGGCAACCATTTGCt                                                                                                                    >  6:167330/1‑148 (MQ=255)
                                   tATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCt                                                                                                                    <  4:170297/147‑1 (MQ=255)
                                   tATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCt                                                                                                                    <  4:176209/147‑1 (MQ=255)
                                   gaTTTACAACAGCACCACCATAGGTTCTGGGCAAAAAGCCTTCATATTCCAGCTTCTCAAGATCGCGGCGAATTGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTTGCAACCATTTGCt                                                                                                                    <  6:25280/146‑1 (MQ=255)
                                     tttAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTaa                                                                                                                 <  3:134233/148‑1 (MQ=255)
                                     tttAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTACAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAt                                                                                                                >  1:312875/1‑149 (MQ=255)
                                       tAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAtt                                                                                                               <  5:167330/148‑1 (MQ=255)
                                        aaaaCATCACCACCATAGGTTCTGGTCAAAAAGCATTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACATGAAAAATCCAACTCAAATTTGAGACTGTCACCTTTTTATCGTTGTCAACCAGTTTATTAATTGc                                                                                                             >  8:48383/1‑149 (MQ=255)
                                        aaaaCAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGc                                                                                                             >  2:347130/1‑149 (MQ=255)
                                         aaaCAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGcc                                                                                                            <  8:242767/149‑1 (MQ=255)
                                          aaCAGCACCACCATAGGTTCTGGTCAAAATGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGGTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCt                                                                                                           <  1:123518/149‑1 (MQ=255)
                                                     caTAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGt                                                                                                                                                 <  1:138785/100‑1 (MQ=255)
                                                     caTAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGt                                                                                                                                                 >  2:138785/1‑100 (MQ=255)
                                                         gggtCTGGTCAAAACGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGACTGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGGCACCTTTTTCTCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCtttt                                                                                             <  5:264357/145‑1 (MQ=255)
                                                           ttCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAACTCCCCCTCAAATTTGAGTCTGTCACCTGTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTgcc                                                                                          >  5:53863/1‑149 (MQ=255)
                                                           ttCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGTCTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTgcc                                                                                          >  5:48705/1‑149 (MQ=255)
                                                                gTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCAc                                                                                     >  8:47841/1‑149 (MQ=255)
                                                                gTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCAc                                                                                     >  1:141378/1‑149 (MQ=255)
                                                                 tCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCAc                                                                                     >  2:128767/1‑148 (MQ=255)
                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGc                                                                                                             >  2:117813/1‑122 (MQ=255)
                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGc                                                                                                             <  1:117813/122‑1 (MQ=255)
                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGAt                                                                                  >  3:316710/1‑149 (MQ=255)
                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGAt                                                                                  >  8:223890/1‑149 (MQ=255)
                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGAt                                                                                  >  7:326781/1‑149 (MQ=255)
                                                                     aaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTgaga                                                                                                                                                     <  6:166611/80‑1 (MQ=255)
                                                                     aaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTgaga                                                                                                                                                     >  5:166611/1‑80 (MQ=255)
                                                                                 ttCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGCATc                                                                    >  4:53449/1‑149 (MQ=255)
                                                                                   ccAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTAt                                                                     <  2:38309/146‑1 (MQ=255)
                                                                                    cAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCttt                                                                 <  4:161297/149‑1 (MQ=255)
                                                                                      gCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCttttt                                                               <  3:290959/149‑1 (MQ=255)
                                                                                       cTTCTCAAGATCGCGGCGATTGGTGTCTTCGGATACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCAGTATCTTTTTa                                                              <  1:158366/149‑1 (MQ=255)
                                                                                          ctcAAGATCGCGGCGAATGGGTTCTTCGGTTACCTGAAAAATCCCACTCACATTTGAGTCTGGCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACAt                                                           <  6:53863/149‑1 (MQ=255)
                                                                                             aaGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGTCTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACAt                                                           <  6:48705/146‑1 (MQ=255)
                                                                                             aaGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATca                                                         >  1:324101/1‑148 (MQ=255)
                                                                                             aaGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATca                                                         >  3:33448/1‑148 (MQ=255)
                                                                                               gATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACAt                                                      >  6:280913/1‑149 (MQ=255)
                                                                                               gATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACAt                                                      >  6:189496/1‑149 (MQ=255)
                                                                                                aTCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACAt                                                      >  8:239105/1‑148 (MQ=255)
                                                                                                 tCGCGGAGAATGGATTCTTCGCTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTa                                                    <  4:233044/149‑1 (MQ=255)
                                                                                                     ggcgAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAgcgc                                                >  6:38700/1‑149 (MQ=255)
                                                                                                      gcgAATGGTTTCTTCGGTTACCTGAAAAATCCCCCTCAACTTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTGTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAgcgc                                                >  2:281538/1‑148 (MQ=255)
                                                                                                      gcgAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAgcgc                                                >  6:94001/1‑148 (MQ=255)
                                                                                                             gTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATc                                         <  1:347130/148‑1 (MQ=255)
                                                                                                             gTTTCTTCGGTTACCTGAAAAATCCAACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATc                                         <  7:48383/148‑1 (MQ=255)
                                                                                                              tttCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGc                                       <  7:239105/149‑1 (MQ=255)
                                                                                                              tttCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTAAATCACATTAGCTCGATTATCg                                        >  1:125707/1‑148 (MQ=255)
                                                                                                                      gtaaCCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGa                               <  2:208737/146‑1 (MQ=255)
                                                                                                                       ttACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGAt                              <  7:339715/149‑1 (MQ=255)
                                                                                                                         aCCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATg                             <  8:326781/148‑1 (MQ=255)
                                                                                                                           cTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGttt                          <  5:189496/149‑1 (MQ=255)
                                                                                                                            tGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATAGTTGGCACCCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGGGATAAACCAATAACCCATGttt                          >  3:189163/1‑148 (MQ=255)
                                                                                                                             gAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTCGCGCGATTATCGCATAACCGATGTTTAc                        >  4:101937/1‑149 (MQ=255)
                                                                                                                             gAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGtt                           >  3:286371/1‑146 (MQ=255)
                                                                                                                             gAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGtt                           <  4:286371/146‑1 (MQ=255)
                                                                                                                             gAAAAATCCCACTCAAATTTGAGACTGGCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTAc                        >  1:297058/1‑149 (MQ=255)
                                                                                                                              aaaaaTCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACt                       <  7:6805/149‑1 (MQ=255)
                                                                                                                                aaaTCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGACTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTGCttt                     <  1:162647/149‑1 (MQ=255)
                                                                                                                                   tCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCaa                  <  7:223890/149‑1 (MQ=255)
                                                                                                                                        cTCAAATTTTATTCTGGAAACTTTTTATCTGTGGCAAACAATTTCTTAATTTGCTGAATACAGGCGTTTGCCGCCACGTTTACACCCCTGTTTATTTTTACATGAACTTAGCGCGATTATCGCATAACCTATGTTTACTTTCAAAATaa             <  4:189163/149‑1 (MQ=255)
                                                                                                                                         tCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAAc            >  5:40939/1‑149 (MQ=255)
                                                                                                                                           aaaTTTGATACTGTCCCCTTTTTATCGTTGGCAACCAGTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGAGTACACCCCTGTATCTTTTTCCATAACATTAGCGCGATTATCGCATACCCGATGTTTACTTTCAAAATAACCt          >  4:315391/1‑149 (MQ=255)
                                                                                                                                           aaaTTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGa                                                                                   <  5:77235/76‑1 (MQ=255)
                                                                                                                                           aaaTTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACCCCCCTGTATCTTTTTACATACCATTAGCGCGATTATCGCATAACAGATGTTTACTTTCAAAATAACCt          >  5:73995/1‑149 (MQ=255)
                                                                                                                                           aaaTTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCAGAATCCAGTCTTTAGCCGCCACGa                                                                                   >  6:77235/1‑76 (MQ=255)
                                                                                                                                              tttGAGACTGTCACCTTTATATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTATTTTGCCGCCACGATTACACCCCTTAATCTTTTTACATCACATTAGCGCGATTATCGCATAACAGATCTTTACTTTCAAAATAACCTCtt       >  2:220252/1‑149 (MQ=255)
                                                                                                                                                  agaCTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGaa   <  2:324101/149‑1 (MQ=255)
                                                                                                                                                   gaCTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAAt  <  6:40939/149‑1 (MQ=255)
                                                                                                                                                    aCTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAATGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATc                                         <  6:53838/109‑1 (MQ=255)
                                                                                                                                                    aCTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAATGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATc                                         >  5:53838/1‑109 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAAT  >  NC_000913/1854642‑1854937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: