Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,933,569 G→A 5.9% H346Y (CAC→TAC)  edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,933,5690GA 5.9% 212.9 / 2.5 68H346Y (CAC→TAC) edd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (34/30);  new base A (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTG  >  NC_000913/1933422‑1933705
                                                                                                                                                   |                                                                                                                                        
gCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTgg                                                                                                                                         <  7:27856/149‑1 (MQ=255)
    gtccagtTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTaa                                                                                                                                     >  5:49554/1‑149 (MQ=255)
          ttCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCgg                                                                                                                               <  7:365601/149‑1 (MQ=255)
            cACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCGGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGc                                                                                                                              <  5:52070/148‑1 (MQ=255)
               cATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATAGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACCCCACATGCCGCCTGGAAGAGGATAATATCGGCCGGa                                                                                                                          >  6:199736/1‑149 (MQ=255)
                       aGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                                                                                     >  5:262497/1‑146 (MQ=255)
                       aGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                                                                                     <  6:262497/146‑1 (MQ=255)
                       aGCCATGGGTCAAGGGTATAACGAGACAGACCAAAACCTGCAACCGTATTGGCATCTTCATGCAGCAGGCCTGCTTTGAGAAGTTCACGCACCCGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCgg                                                                                                                  <  3:101102/149‑1 (MQ=255)
                          aatgctgcAAGGGTATAACGAGACAGTCCAAAACCTGCCACCGTATTGACATCTTCATGCAGCTGGCCTGCTTTGAGGCGTTCACGCACCTGAACCGGTACGCGACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTa                                                                                                               <  5:78778/142‑1 (MQ=255)
                           aTGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                                                                                     <  5:332948/142‑1 (MQ=255)
                           aTGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                                                                                     >  6:332948/1‑142 (MQ=255)
                                  aaGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                                                                                     >  1:31114/1‑135 (MQ=255)
                                  aaGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                                                                                     <  2:31114/135‑1 (MQ=255)
                                  aaGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGt                                                                                                        <  6:49554/148‑1 (MQ=255)
                                   aGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGc                                                                                                      >  5:238501/1‑149 (MQ=255)
                                     ggTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCa                                                                                                    >  3:165632/1‑149 (MQ=255)
                                            cGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGGCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCgg                                                                                             <  4:239502/149‑1 (MQ=255)
                                                accgacCAAAACCTGCAACCGTATTGAAATCTTAATGCAGCATGCCTGCTTTGATCAGGTCACGCACCAGAACCGGTACGCAACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGTTAGATCCTTGCCATCAGCGGTac                                                                                          >  2:136466/4‑148 (MQ=255)
                                                 cagacCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTaca                                                                                         <  1:58220/148‑1 (MQ=255)
                                                    acCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCACCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACAt                                                                                     >  1:99236/1‑149 (MQ=255)
                                                      cAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACAt                                                                                     >  4:82970/1‑147 (MQ=255)
                                                       aaaaCCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTTAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTAGTTAATATCGGACGGACCGTTAGGGTAGAGACGTGCCATAAGCGGTACAACAt                                                                                     >  4:232393/1‑146 (MQ=255)
                                                       aaaaCCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAg                                                                                  <  4:212309/149‑1 (MQ=255)
                                                       aaaaCCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTAGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACAt                                                                                     >  4:237413/1‑146 (MQ=255)
                                                         aaCCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGaa                                                                                <  2:218944/149‑1 (MQ=255)
                                                           ccTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAg                                                                              <  3:82970/149‑1 (MQ=255)
                                                                 aCCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCa                                                                          >  7:353869/1‑147 (MQ=255)
                                                                     tATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAg                                                                    <  7:68956/149‑1 (MQ=255)
                                                                     tATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATAGCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAg                                                                    <  7:395757/149‑1 (MQ=255)
                                                                        tGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCa                                                                 >  7:242719/1‑149 (MQ=255)
                                                                         gACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCa                                                                 <  8:242719/148‑1 (MQ=255)
                                                                         gACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCAt                                                                >  2:298625/1‑149 (MQ=255)
                                                                          aCATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATc                                                               >  2:148152/1‑149 (MQ=255)
                                                                          aCATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATc                                                               >  3:21983/1‑149 (MQ=255)
                                                                           cATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACATCATCAGAAAGGTCAGAGAAGTCATcc                                                              <  8:353869/149‑1 (MQ=255)
                                                                           cATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGaa                                                                     >  7:396872/1‑142 (MQ=255)
                                                                           cATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGaa                                                                     <  8:396872/142‑1 (MQ=255)
                                                                            aTCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACAGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGAAGTGCCATCAGCGGTACTACATCAGAAAGGTCAGAGAAGTCATca                                                              >  5:221239/1‑147 (MQ=255)
                                                                            aTCTTAATGCAGCAGGCCTGCTTTGAGCAGTTCACGAACAAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGTTATAGACGTTCCATAAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATcc                                                              <  6:221239/148‑1 (MQ=255)
                                                                             tCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCTGCCGGACCCTTCTGGTAGAGACGTGCCGTCAGCCGTACAACATCATAAAGGTCAGATAAGTCATcc                                                              >  6:47387/1‑147 (MQ=255)
                                                                                 cATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAg                                                           <  6:238501/146‑1 (MQ=255)
                                                                                   tGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGTAATTGGTTAATAGCGGCAGGACCGTTCGGGTAGAGAAGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAAt                                                      <  5:199736/149‑1 (MQ=255)
                                                                                    gcagcaGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATc                                                     >  4:251683/1‑149 (MQ=255)
                                                                                         agGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGaa                                                 >  1:14429/1‑148 (MQ=255)
                                                                                         agGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCACCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAAt                                                <  2:99236/149‑1 (MQ=255)
                                                                                           gCCTGCTTTGAGCAGTTCACGCACCAGCACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATAc                                              >  5:102316/1‑149 (MQ=255)
                                                                                           gCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATc                                                                                    <  6:19962/111‑1 (MQ=255)
                                                                                           gCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATc                                                                                    >  5:19962/1‑111 (MQ=255)
                                                                                            ccTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATAcc                                             >  3:221973/1‑149 (MQ=255)
                                                                                             cTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCg                                            >  3:36796/1‑149 (MQ=255)
                                                                                              tGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATACCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCgg                                           >  2:289843/1‑149 (MQ=255)
                                                                                               gCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGc                                          >  4:187960/1‑149 (MQ=255)
                                                                                               gCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGc                                          >  4:181970/1‑149 (MQ=255)
                                                                                                cTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGcc                                         >  8:342100/1‑149 (MQ=255)
                                                                                                 tttGAGCAGTCCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCcg                                        >  8:32427/1‑149 (MQ=255)
                                                                                                     aGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATACCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCcgcgc                                     <  1:289843/148‑1 (MQ=255)
                                                                                                             aCGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCg                            >  6:266258/1‑149 (MQ=255)
                                                                                                              cGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGc                           <  3:251683/149‑1 (MQ=255)
                                                                                                                    aGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAg                     >  7:5445/1‑149 (MQ=255)
                                                                                                                                 cACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGtgtg         >  7:361891/1‑148 (MQ=255)
                                                                                                                                 cACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGtgtg         <  8:361891/148‑1 (MQ=255)
                                                                                                                                   ccTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGtt      <  1:298625/149‑1 (MQ=255)
                                                                                                                                    cTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGt       >  2:113120/1‑147 (MQ=255)
                                                                                                                                       ccgccTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGt   <  2:14429/148‑1 (MQ=255)
                                                                                                                                       ccgccTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTg  <  4:165632/149‑1 (MQ=255)
                                                                                                                                       ccgccTGGAAGTAGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTg  <  3:237413/149‑1 (MQ=255)
                                                                                                                                       cagccTGGAAGTAGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGACAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTg  <  3:232393/147‑1 (MQ=255)
                                                                                                                                              gAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCt                                                    <  1:65990/92‑1 (MQ=255)
                                                                                                                                              gAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCt                                                    >  2:65990/1‑92 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
GCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTG  >  NC_000913/1933422‑1933705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑