Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 564,107 IS2 (+) +5 bp coding (370‑374/633 nt) sfmZ ← response regulator family protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)34 (0.390) 31/284 1.4 97.0% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 564111 1 (0.010)coding (370/633 nt) sfmZ response regulator family protein
* ? NC_000913 = 382590NA (NA)71 (0.810) 52/286 0.5 98.6% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 564107 = 1 (0.010)coding (374/633 nt) sfmZ response regulator family protein

GGCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTG  >  NC_000913/563968‑564258
                                                                                                                                               |                                                                                                                                                   
ttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCg                                                                                                                                                 >  5:185392‑M2/140‑148 (MQ=255)
  gcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCg                                                                                                                                                 >  8:76066‑M2/138‑146 (MQ=255)
  gcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCg                                                                                                                                                 <  7:76066‑M2/9‑1 (MQ=255)
   cagaggcgttcgagcattataacgaatggcatccgcatagttcgctgggttatcgctcgccacgggaatatctgcggcagcggggttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGgaga                                                                                                                                             <  5:8236‑M2/13‑1 (MQ=255)
   cagaggcgttcgagcattataacgaatcgcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGgaga                                                                                                                                             <  2:161189‑M2/13‑1 (MQ=255)
   aaTTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactgg                                                                                                                                             <  5:254552‑M2/149‑9 (MQ=255)
     gaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGagag                                                                                                                                            >  3:173184‑M2/135‑148 (MQ=255)
     gaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAgg                                                                                                                                           <  8:265686‑M2/15‑1 (MQ=255)
          gttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGatcat                                                                                                                                      >  4:85483‑M2/130‑149 (MQ=255)
          gttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGatcat                                                                                                                                      <  3:257963‑M2/20‑1 (MQ=255)
          gttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGatcat                                                                                                                                      >  7:138588‑M2/130‑149 (MQ=255)
            attaGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGAGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaa                                                                                                                                    <  7:137752‑M2/149‑18 (MQ=255)
              gagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCt                                                                                                                                    >  4:231519‑M2/126‑147 (MQ=255)
              gagcattataacgaatggcatccgcatagtgagctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCt                                                                                                                                    <  3:231519‑M2/22‑1 (MQ=255)
                  tAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctcca                                                                                                                              >  3:53201‑M2/1‑126 (MQ=255)
                    aTCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTTTATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTATCCGGAGAGGATCATCTGAACGGc                                                                                                                             <  5:64131/148‑1 (MQ=255)
                        gCTAACCAGATAACGTAATATCGGCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgactctccagacaac                                                                                                                        >  2:84566‑M2/1‑120 (MQ=255)
                          gaatggcatccgcatagtgcgctaggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGaaa                                                                                                                       >  8:352980‑M2/114‑148 (MQ=255)
                            atggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGaaaaa                                                                                                                     >  8:236140‑M2/112‑148 (MQ=255)
                            aCCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaat                                                                                                                    >  6:153173‑M2/1‑116 (MQ=255)
                               gcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagggggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAatat                                                                                                                  >  1:328609‑M2/109‑148 (MQ=255)
                               gcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAatat                                                                                                                  <  2:168243‑M2/40‑1 (MQ=255)
                               gcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATc                                                                                                                 <  6:157884‑M2/41‑1 (MQ=255)
                                gATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatca                                                                                                                >  5:32836‑M2/1‑112 (MQ=255)
                                catccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCa                                                                                                                >  1:147322‑M2/108‑149 (MQ=255)
                                catccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCa                                                                                                                <  8:180488‑M2/42‑1 (MQ=255)
                                  tccgcatagtgcgctgggttatcgctcgccacgggaatatctgctgcatctggattgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCAtt                                                                                                              <  7:134878‑M2/44‑1 (MQ=255)
                                  tccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCAtt                                                                                                              <  5:171356‑M2/44‑1 (MQ=255)
                                  tccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCAtt                                                                                                              <  2:147322‑M2/44‑1 (MQ=255)
                                    cgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagggggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTc                                                                                                             <  2:328609‑M2/45‑1 (MQ=255)
                                    cgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTc                                                                                                             <  5:312689‑M2/45‑1 (MQ=255)
                                    cgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTc                                                                                                             <  1:37899‑M2/45‑1 (MQ=255)
                                    cgcatagggcgctgggttatctctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggcgcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTc                                                                                                             <  6:47760‑M2/45‑1 (MQ=255)
                                    aCGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcactt                                                                                                             >  1:151967‑M2/1‑108 (MQ=255)
                                       atagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGAt                                                                                                         <  2:304468‑M2/49‑1 (MQ=255)
                                           tgcgctggtttatcgctcgccacgggaatatctgcggctgcgggcttgtaatggtttaagtgataacagatgtctggaaatattggggcatatccaGTGTATCGGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCAtt                                                                                                     <  4:231532‑M2/53‑1 (MQ=255)
                                           tgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatatagtggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCAtt                                                                                                     <  2:12590‑M2/53‑1 (MQ=255)
                                           tgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggtgcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCAtt                                                                                                     <  1:83642‑M2/53‑1 (MQ=255)
                                           tgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCAtt                                                                                                     <  3:112295‑M2/53‑1 (MQ=255)
                                              gTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtga                                                                                                   <  8:27768‑M2/148‑51 (MQ=255)
                                              gTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtga                                                                                                   >  5:279140‑M2/1‑98 (MQ=255)
                                               tCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgata                                                                                                 >  3:287238‑M2/1‑97 (MQ=255)
                                                 gggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCAtt                                                                                                >  8:351305‑M2/91‑148 (MQ=255)
                                                 gggttatcgctcgccaccggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTAGCCGTAGAGGATCATCTGACCGGCATGAAAAATATCATACTGATCATTGAAtt                                                                                                >  5:91443‑M2/91‑148 (MQ=255)
                                                   gttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTAc                                                                                             <  2:358843‑M2/61‑1 (MQ=255)
                                                    ttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACt                                                                                            <  8:315453‑M2/62‑1 (MQ=255)
                                                          ctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACaa                                                                                       >  4:206710‑M2/82‑148 (MQ=255)
                                                          ctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACaa                                                                                       >  1:156046‑M2/82‑148 (MQ=255)
                                                             gATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagt                                                                                   <  3:78010‑M2/149‑67 (MQ=255)
                                                               cacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGt                                                                                 <  7:236140‑M2/73‑1 (MQ=255)
                                                               cacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGt                                                                                 <  3:43676‑M2/73‑1 (MQ=255)
                                                                 cgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTa                                                                               <  3:122808‑M2/75‑1 (MQ=255)
                                                                    gaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCa                                                                            >  3:46042‑M2/72‑149 (MQ=255)
                                                                        gACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttgatactagtttttagactag                                                                        <  4:100440‑M2/149‑78 (MQ=255)
                                                                        atctgcggaagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCAc                                                                           >  6:153851‑M2/68‑146 (MQ=255)
                                                                            gTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcat                                                                    <  7:268114‑M2/149‑82 (MQ=255)
                                                                             ttGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcatt                                                                   <  1:105696‑M2/149‑83 (MQ=255)
                                                                             cggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGc                                                                       <  8:138588‑M2/83‑1 (MQ=255)
                                                                             cggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCtt                                                                     <  7:351305‑M2/85‑1 (MQ=255)
                                                                             cggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGt                                                                   <  6:185392‑M2/87‑1 (MQ=255)
                                                                               gAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttgatactagtttttagactagtcattg                                                                  >  3:100440‑M2/1‑65 (MQ=255)
                                                                                aaCTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattg                                                                  <  8:210459‑M2/147‑84 (MQ=255)
                                                                                 aCTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattgga                                                                >  8:268114‑M2/1‑63 (MQ=255)
                                                                                   tATTCGTACTACATTTATTGCTTTTTATATAGTTAATCGTTTCTCTGGGAAAAAACGTGTAtagactggccacctgaatctccagacaaccaatataacttaaataagtgatagtcttaatactagtttttagactagtcattggagaa                                                             <  1:119882‑M2/149‑89 (MQ=255)
                                                                                          gtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCa                                                       <  7:352980‑M2/99‑1 (MQ=255)
                                                                                          gtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAg                                                      >  3:88739‑M2/50‑149 (MQ=255)
                                                                                           ctacATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgat                                                     <  4:201566‑M2/149‑97 (MQ=255)
                                                                                            tacATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgatt                                                    <  8:202691‑M2/149‑98 (MQ=255)
                                                                                            tacATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccacctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgagt                                                    <  6:273371‑M2/149‑98 (MQ=255)
                                                                                            aatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCa                                                    >  8:178244‑M2/48‑149 (MQ=255)
                                                                                              cATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgatt                                                    >  7:210459‑M2/1‑50 (MQ=255)
                                                                                                    ttGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtctt                                            >  8:225469‑M2/1‑44 (MQ=255)
                                                                                                       cTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagg                                         <  8:66329‑M2/149‑109 (MQ=255)
                                                                                                       cTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagg                                         <  2:36189‑M2/149‑109 (MQ=255)
                                                                                                       cTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttag                                          >  6:254552‑M2/1‑41 (MQ=255)
                                                                                                           aacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGAt                                     >  3:218292‑M2/33‑149 (MQ=255)
                                                                                                             cagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCCGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGAttt                                   <  4:218292‑M2/119‑1 (MQ=255)
                                                                                                             atataGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccg                                     <  7:108125‑M2/147‑113 (MQ=255)
                                                                                                               gatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCg                                 <  2:156046‑M2/121‑1 (MQ=255)
                                                                                                                 tgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgat                               <  4:88739‑M2/123‑1 (MQ=255)
                                                                                                                   tctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatga                             <  3:325116‑M2/125‑1 (MQ=255)
                                                                                                                     tggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCgatgat                            >  1:124127‑M2/23‑148 (MQ=255)
                                                                                                                         aatataggggctaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCAGCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATaaaaa                       <  6:91443‑M2/131‑1 (MQ=255)
                                                                                                                            tCGCTGGGAAAAAACGTGTAttgactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggc                     >  7:66329‑M2/1‑20 (MQ=255)
                                                                                                                               ggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAaca                  <  4:46042‑M2/136‑1 (MQ=255)
                                                                                                                               ggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCAGATTTCGATGATAAAAATAacac                 >  3:240459‑M2/13‑149 (MQ=255)
                                                                                                                                 ggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCAtt                                          >  8:196998‑M2/11‑122 (MQ=255)
                                                                                                                                 ggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCAtt                                          <  7:196998‑M2/112‑1 (MQ=255)
                                                                                                                                  gcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACttt              >  1:138225‑M2/10‑149 (MQ=255)
                                                                                                                                   caaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTc             <  2:138225‑M2/141‑1 (MQ=255)
                                                                                                                                    aaaaaaCGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccaca             <  5:144756‑M2/148‑137 (MQ=255)
                                                                                                                                     aatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCAc           >  4:184571‑M2/7‑149 (MQ=255)
                                                                                                                                      aaaaCGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacag          <  7:368962‑M2/149‑140 (MQ=255)
                                                                                                                                       aaaCGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcatacggcgtaccacacag          <  7:212897‑M2/148‑140 (MQ=255)
                                                                                                                                             gtAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaa       <  3:92243‑M2/145‑143 (MQ=255)
                                                                                                                                             gtAtagactggccccctgaatctccagaaaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcatttgagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaaag    <  8:350097‑M2/148‑146 (MQ=255)
                                                                                                                                              tatagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaaaga   >  2:105696‑M2/1‑2 (MQ=255)
                                                                                                                                              tATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTg  <  3:184571/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTG  >  NC_000913/563968‑564258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.