Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 756,623 G→A W239* (TGG→TGA sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,6230GA98.7% 265.6 / ‑5.5 78W239* (TGG→TGAsdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (42/35);  minor base C (0/1);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCG  >  NC_000913/756478‑756763
                                                                                                                                                 |                                                                                                                                            
tttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAg                                                                                                                                           >  4:328143/1‑149 (MQ=255)
tttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAg                                                                                                                                           <  4:166269/149‑1 (MQ=255)
 ttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGt                                                                                                                                          >  6:77808/1‑149 (MQ=255)
  tATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGtt                                                                                                                                         >  5:75442/1‑149 (MQ=255)
       cAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAcc                                                                                                                                    >  7:185141/1‑149 (MQ=255)
       cAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAc                                                                                                                                     <  6:18639/148‑1 (MQ=255)
       cAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAc                                                                                                                                     <  3:232277/148‑1 (MQ=255)
       cAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCAACAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAcc                                                                                                                                    >  6:96553/1‑149 (MQ=255)
           gCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGAc                                                                                                                                <  5:77808/149‑1 (MQ=255)
            cccGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCCCCACCAACGCCCACATTAACACCGGCGCCGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGAGCAGGATATGGAAATGTGACAGTTCCACCCGAcc                                                                                                                               >  2:359587/1‑149 (MQ=255)
            cccGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGAcc                                                                                                                               >  8:122106/1‑149 (MQ=255)
                 gcTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGTGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAc                                                                                                                                     >  2:185458/1‑138 (MQ=255)
                 gcTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGTGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAc                                                                                                                                     <  1:185458/138‑1 (MQ=255)
                     ccGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTg                                                                                                                        <  7:158778/147‑1 (MQ=255)
                     ccGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGc                                                                                                                       <  2:66414/148‑1 (MQ=255)
                      cGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCg                                                                                                                     <  8:185141/149‑1 (MQ=255)
                              cGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGc                                                                                                             <  3:133413/149‑1 (MQ=255)
                               gACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCg                                                                                                            >  2:82567/1‑149 (MQ=255)
                                 cTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTa                                                                                                          <  7:100633/149‑1 (MQ=255)
                                    gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTg                                                                                                       >  7:38248/1‑149 (MQ=255)
                                    gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTg                                                                                                       >  7:224141/1‑149 (MQ=255)
                                    gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTg                                                                                                       >  1:210924/1‑149 (MQ=255)
                                     cGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTgg                                                                                                      >  3:229647/1‑149 (MQ=255)
                                       gAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGt                                                                                                     >  6:14129/1‑148 (MQ=255)
                                         gCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCAc                                                                                                  <  2:197669/149‑1 (MQ=255)
                                            gggCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAc                                                                                                                                     >  8:13683/1‑111 (MQ=255)
                                            gggCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCAc                                                                                                                                     <  7:13683/111‑1 (MQ=255)
                                              gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAg                                                                                             >  7:2660/1‑149 (MQ=255)
                                               cGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGTCGACGTTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGAAATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAgg                                                                                            <  2:360599/149‑1 (MQ=255)
                                                   tttATCAGTCAACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCTGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGc                                                                                        >  6:217364/1‑149 (MQ=255)
                                                      aTCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGt                                                                                                                   <  4:254574/119‑1 (MQ=255)
                                                      aTCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgt                                                                                     >  6:205712/1‑149 (MQ=255)
                                                       tCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTTGCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtg                                                                                    <  5:61651/149‑1 (MQ=255)
                                                              accaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAgg                                                                             >  1:339766/1‑149 (MQ=255)
                                                              accaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAgg                                                                             <  2:154989/149‑1 (MQ=255)
                                                                       gCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATct                                                                    <  7:13466/149‑1 (MQ=255)
                                                                        cccACATTAACACCGGCGACGGTGTGGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTTGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctg                                                                   <  8:185696/149‑1 (MQ=255)
                                                                         ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAgg                                                                                            >  3:284192/1‑123 (MQ=255)
                                                                         ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAgg                                                                                            <  4:284192/123‑1 (MQ=255)
                                                                         ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                  >  2:234509/1‑149 (MQ=255)
                                                                          cacaTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgct                                                                 <  3:328143/149‑1 (MQ=255)
                                                                           acaTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgctg                                                                <  1:82567/149‑1 (MQ=255)
                                                                           acaTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgct                                                                 >  7:243385/1‑148 (MQ=255)
                                                                            caTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGa                                                               >  7:306634/1‑149 (MQ=255)
                                                                                  cacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaa                                                          >  6:58017/1‑148 (MQ=255)
                                                                                   acCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaaca                                                        >  3:53775/1‑149 (MQ=255)
                                                                                    ccGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACAt                                                       >  1:161956/1‑149 (MQ=255)
                                                                                    ccGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGGGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACAt                                                       <  2:337320/149‑1 (MQ=255)
                                                                                     cGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATg                                                      <  5:96553/149‑1 (MQ=255)
                                                                                     cGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATg                                                      <  4:231917/149‑1 (MQ=255)
                                                                                     cGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATg                                                      >  8:367178/1‑149 (MQ=255)
                                                                                      ggCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATgg                                                     <  8:224141/149‑1 (MQ=255)
                                                                                        cGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGc                                                    >  4:143327/1‑148 (MQ=255)
                                                                                           cGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAAc                                                <  4:53775/149‑1 (MQ=255)
                                                                                                      tGGCTCTCCGTTCCGGCGTACCGGTGCAGTATATGGAACTGTGCCATTTCAACCCGACCGGCTTTGGCGGTTCTGGCTTACTTTTAACCGATGTTTGACGTTGTGAAAGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAg                                     <  5:108298/149‑1 (MQ=255)
                                                                                                       ggCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGc                                    >  6:26873/1‑149 (MQ=255)
                                                                                                        gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                  <  4:290163/102‑1 (MQ=255)
                                                                                                        gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                  >  3:290163/1‑102 (MQ=255)
                                                                                                        gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCg                                   >  7:10062/1‑149 (MQ=255)
                                                                                                          tATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGATCGt                                  >  1:72429/1‑148 (MQ=255)
                                                                                                            tCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTAt                               >  6:49639/1‑149 (MQ=255)
                                                                                                            tCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTAt                               <  2:60207/149‑1 (MQ=255)
                                                                                                              cGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATgc                             <  6:75442/149‑1 (MQ=255)
                                                                                                               gTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATgcg                            >  6:257337/1‑149 (MQ=255)
                                                                                                                   cGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCg                         >  6:279430/1‑148 (MQ=255)
                                                                                                                   cGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCg                         >  1:266581/1‑148 (MQ=255)
                                                                                                                       gTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGc                    <  2:161956/149‑1 (MQ=255)
                                                                                                                        tACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGcc                   <  5:58017/149‑1 (MQ=255)
                                                                                                                               gCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaac                                                         >  8:355491/1‑104 (MQ=255)
                                                                                                                               gCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaac                                                         <  7:355491/104‑1 (MQ=255)
                                                                                                                               gCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAAc                      >  1:180529/1‑139 (MQ=255)
                                                                                                                               gCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAAc                      <  2:180529/139‑1 (MQ=255)
                                                                                                                                     tatGGAAATGTGACAGTGCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCgg      <  4:14737/149‑1 (MQ=255)
                                                                                                                                      atGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCggg     >  6:30185/1‑149 (MQ=255)
                                                                                                                                       tGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGc    <  4:229647/149‑1 (MQ=255)
                                                                                                                                        ggAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGACGGCGGTTATCTGCTGAGCAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGcc   >  3:69635/1‑149 (MQ=255)
                                                                                                                                        ggAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGcc   >  3:172116/1‑149 (MQ=255)
                                                                                                                                         gAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCg  >  2:229606/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCG  >  NC_000913/756478‑756763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: