Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	757,731	Δ1 bp	coding (43/717 nt)	sdhB →	succinate dehydrogenase, FeS subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	757,731	0	G	.	100.0%	67.5 / NA	16	coding (43/717 nt)	sdhB	succinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (7/9);  total (7/9)

CCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGC  >  NC_000913/757587‑757832                                                                                                                                                 |                                                                                                      ccAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTt‑atgat                                                                                                  >  2:122319/1‑149 (MQ=255)                            aGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTg                                                                       <  1:127105/149‑1 (MQ=255)                                           ccGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATg                                                             <  1:103517/144‑1 (MQ=255)                                           ccGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATg                                                             >  2:103517/1‑144 (MQ=255)                                              aaaCTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTcgc                                                     <  1:122319/149‑1 (MQ=255)                                                                  gccgAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATgc                                 >  2:178878/1‑149 (MQ=255)                                                                   ccgAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATgcg                                >  7:40223/1‑149 (MQ=255)                                                                              tacttactAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATgcgc                               >  1:67242/1‑139 (MQ=255)                                                                              tacttactAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATgcgc                               <  2:67242/139‑1 (MQ=255)                                                                                   actAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTaaaa                 <  8:40223/148‑1 (MQ=255)                                                                                     tAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATgcgc                               >  2:188151/1‑132 (MQ=255)                                                                                     tAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATgcgc                               <  1:188151/132‑1 (MQ=255)                                                                                                    aaaaTGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCa                        <  5:65032/124‑1 (MQ=255)                                                                                                    aaaaTGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCa                        >  6:65032/1‑124 (MQ=255)                                                                                                              tCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGc  >  7:17481/1‑136 (MQ=255)                                                                                                              tCGAGTTTTCAATTTATCGCTATAACCCGGATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGc  <  8:17481/136‑1 (MQ=255)                                                                                                                                           gATGTT‑ATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCtt                             <  8:108185/80‑1 (MQ=255)                                                                                                                                                 |                                                                                                      CCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGCTATAACCCGGATGTTGATGATGCTCCGCGTATGCAGGATTACACCCTGGAAGCGGATGAAGGTCGCGACATGATGCTGCTGGATGCGCTTATCCAGCTAAAAGAGAAAGATCCCAGC  >  NC_000913/757587‑757832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑