Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,004,628 A→G 6.8% S228G (AGT→GGT)  ycbF → putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,004,6280AG 6.8% 442.4 / 2.2 162S228G (AGT→GGT) ycbFputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (63/83);  new base G (12/2);  total (77/85)
Fisher's exact test for biased strand distribution p-value = 3.56e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

GCGATGCGCTCGGTATTTAAACTGTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGT  >  NC_000913/1004349‑1004907
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           
         tgtgtttttAAACTGTTCTGGCGAAACGAAGGGTTGCCGAGCGACCCGCTGGAAGCTTATCAACAATTATGTTGAAAACGGAATTCGCAGAGTGTACAACTCTCTTACCCAACGCCTTATTACATTAACCTGATTCAAGTGAGTGTAAATAGTATAGCGGTAAGTATTGTGGGATTTGGGCCGCCTAAAAGCCCTCGTCAAACAAGCTGGTGTCAGGCAATCGCTCCGTGTCAAATCGCGTGTCGGGCGATAAAAGATTACGGCGGTTTGAGTGCAaagaa                                                                                                                                                                                                                                                                                  <  2:1163580/275‑1 (MQ=255)
                    aCTGTTCTGGCGACCCAAAGGGTTGCAGAGAGACCCGCTGGAAGCTAATCAAAAATTACCCATGAGACGGAAGTCGCAGTGTGTACAACTCACTAACCCAACGCCTTATTACATTATCCTGGTTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTATGCCACCTAAAAGCGAGCGTCAGACAAGCTGGTGTCAGGCAAGCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCagcatc                                                                                                                                                                                                                                                                       <  2:488585/281‑6 (MQ=255)
                     cTGTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTg                                                                                                                                                                                                                                                                     <  1:467721/282‑1 (MQ=255)
                       gTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTTCAAAGAAGGAGAAAAATTTGcc                                                                                                                                                                                                                                                                   >  1:1828623/1‑282 (MQ=255)
                       gTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGcc                                                                                                                                                                                                                                                                   >  1:647958/1‑282 (MQ=255)
                       gTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGcc                                                                                                                                                                                                                                                                   >  1:1354645/1‑282 (MQ=255)
                       gTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGGCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGGAAAGAAGGAGCAAAATCTGcc                                                                                                                                                                                                                                                                   >  1:1714177/1‑282 (MQ=255)
                       gTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGACAGCGTCAGACAAGCTGGTGTCAGGAAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCACAGAAGGGGCaaaaagcgcc                                                                                                                                                                                                                                                                   >  1:1593788/1‑276 (MQ=255)
                                aCCCGAAGGTCTGCCGGGCGACCCGCTGGAAGCTTACCAACAATTACGCTGGACACGGAACTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAACTGGTAAAGCGTTAAGTAATGTGGGATTTGTGCCGACTAAAAGCCAGGCTCAGACAAGCTGGGGTAAGGCAATCGCACGGTTTCATGTCGCCTGGCTGGGGCTAAATGATTACGGCTGGGATGGGTGCAAGGACGGCGAAAAATCGCCCTTCAACaa                                                                                                                                                                                                                                                         >  2:271655/1‑281 (MQ=255)
                                  ccGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGGAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGGGTCATGTCGCCTGGCGGGCGAGAAATGATTACGGCGGGGGTGGTGTCAAGAAAGGAGCAAAATCTGccgcacacaggg                                                                                                                                                                                                                                                         >  2:1846522/1‑272 (MQ=255)
                                           tGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAAc                                                                                                                                                                                                                                              >  1:1835075/1‑282 (MQ=255)
                                                                                   gACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAAt                                                                                                                                                                                                                                                                        <  2:170875/217‑1 (MQ=255)
                                                                                   gACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAAt                                                                                                                                                                                                                                                                        >  1:170875/1‑217 (MQ=255)
                                                                                   gACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTc                                                                                                                                                                                                      <  1:1949637/282‑1 (MQ=255)
                                                                                         gAATTCCCAGGGTGTACAACTCACTAACCCCACGCCCTATTACATTAACCTTATGCGGGGGAGTGTAAATGGTAAAGCGTTAAGTAAGTTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCAGCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGGGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACt                                                                                                                                                                                                <  2:368586/282‑1 (MQ=255)
                                                                                             tCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTTCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGc                                                                                                                                                                                             >  1:561730/1‑281 (MQ=255)
                                                                                             tCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGGTTGCGGTTTTTTTTCCGCAGGTCAATTCCCTTTTTGTCCGAACTCGca                                                                                                                                                                                            >  2:1306048/1‑282 (MQ=255)
                                                                                                 aGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACat                                                                                                                                                                                        <  1:131657/281‑1 (MQ=255)
                                                                                                  gggTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGGAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACataa                                                                                                                                                                                      <  1:1387930/282‑1 (MQ=255)
                                                                                                    gtgtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTTGGTGCa                                                                                                                                                                                                                                                                                       >  2:1868917/1‑185 (MQ=255)
                                                                                                       tACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGTGCAAAGGAGGAGCCAAATCTGCCCTGAAAAAGGTTTGGGAAACGTTTGCGGTTTTTTTCGCGCATGTCAATTCCCTTTTGGTTCGCACTCGCACATTATAAGc                                                                                                                                                                                 >  1:1922164/1‑282 (MQ=255)
                                                                                                             tCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCg                                                                                                                                                                                                                           <  2:1185377/235‑1 (MQ=255)
                                                                                                               aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTTAGTGAAAAGAAGGAGCAAAAACTGCCCTTAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTTCCTTTTGGTCCGAACTCGGCCATAATACTCCCCCggc                                                                                                                                                                          >  1:2614/1‑280 (MQ=255)
                                                                                                               aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATc                                                                                                                                                                                                                                                                       >  2:1563092/1‑190 (MQ=255)
                                                                                                               aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATc                                                                                                                                                                                                                                                                       <  1:1563092/190‑1 (MQ=255)
                                                                                                               aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCCGGTTAATTCCCTTTTTGTCCGAACTCCCACATAATACGCCCCCggg                                                                                                                                                                          >  1:613822/1‑280 (MQ=255)
                                                                                                               aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGca                                                                                                                                                                                            <  2:1603723/264‑1 (MQ=255)
                                                                                                               aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAAACTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTTCCTTTTGGTCCCAACTCCCACCTAATTCGCCCCCGGt                                                                                                                                                                          >  1:2002969/1‑281 (MQ=255)
                                                                                                               aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGGTCAAAAAAGGAGGCAAATCTGCCCTGAAAAAAGTTCGGAAAACGGTTGCGTTTTTTTTTCCGCAGGGCAAATTCCTTTTTGTCCGAACACGCCCACAATAACCCCCCGGt                                                                                                                                                                          >  2:1961089/1‑281 (MQ=255)
                                                                                                                 tAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGGGGGCAAAGAAGGAGCAAAATCTGCCCTGAAAACGGTTTGGAAAACGGTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAAATCCCCCAAAATACCCCCCCGGTTTg                                                                                                                                                                       >  2:751550/1‑282 (MQ=255)
                                                                                                                      cAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACg                                                                                                                                                                                                                                             <  1:820938/208‑1 (MQ=255)
                                                                                                                       aaCGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGGGTGCAAAGAAGGAGAAAAAACTGACCTGAAAAAGGGTCGGAAAACGTGTGCGGTTTTTTTGCCGCAGGGCAATTCCCCTTTGGGCCGAACTCGCACATAATACTCCCCCGGGTTgtaacc                                                                                                                                                                  >  2:1174595/1‑276 (MQ=255)
                                                                                                                        aCGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCg                                                                                                                                                                >  1:360933/1‑282 (MQ=255)
                                                                                                                         cGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCgg                                                                                                                                                               <  1:1474360/282‑1 (MQ=255)
                                                                                                                          gCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACt                                                                                                                                                                                                <  1:1275890/249‑1 (MQ=255)
                                                                                                                            cTTATTACATTAAACTGATTCAGGGTAGTGTAAATGGTAAAGCGTTAAGGAATTGGGGAGTTGTGGCGCCTAAAAGCCTGCGTCAAACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCCCCTGGCCGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGcac                                                                                                                                                                    <  2:1498381/274‑1 (MQ=255)
                                                                                                                              tattaCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATc                                                                                                                                                          <  1:1306048/282‑1 (MQ=255)
                                                                                                                              tattaCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGCTAAGTAATGGGGGAGATGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCATGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAACTGTTTACGGCTGGTTCAGTGCTAAAAAAGAGCAAAAGCGGCCCTTAAACCGGGTTTGGAAAAGTTTGCCGTTTTTTTTCCCTACGTCAATTTCCTTTTCGGGCGGACGATCACATACTACCGCCCCCGTTTACATCCCCGGaacc                                                                                                                                                          >  2:312737/1‑280 (MQ=255)
                                                                                                                                   cATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGga                                                                                                                                                     <  1:429388/282‑1 (MQ=255)
                                                                                                                                      ttaCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAATGCAAAGAAGGAGCCAAATCTGCCCTTAAAACGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGGTAATTCCCCTTTTGTCCGAACACCACACAAATACCCCCCCGGTTTTCACCCCGCGTATCCCGGacag                                                                                                                                                  >  2:1683097/3‑282 (MQ=255)
                                                                                                                                      tAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATACATGATTACGGCGGGTTGAGTGCAAAGAAGGCGCCAAATCTGCCCTGAAACAGGTTTGGGAAACGTTTGCGTTTTTTTTTCCGCAGGTAAATTCCCTTTTGGTCCGAACTCGCCCATCATACGCCCACGCATTTCACACCCGGACTCCAGGggag                                                                                                                                                  >  2:1657291/1‑282 (MQ=255)
                                                                                                                                      tAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGCGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGGTTGCGGTTTTTTTGACGCAGGTCAAATCCCTTTTGGGCCGAACTCGCACACAATACGCCCCCGGTTTGCCCACCGGGAATCCAGGagag                                                                                                                                                  >  1:1477277/1‑282 (MQ=255)
                                                                                                                                      tAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCACTGAAAAAGGTTCGGAAAACGTTTGCGTTTTTTTTGGCGCAGGTCAATTCCCTTTTGGTCCGAATTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGagag                                                                                                                                                  >  1:558019/1‑282 (MQ=255)
                                                                                                                                       aaCCTGATTGAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGAAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATATGCCCTGAAACAGGTTCGGAAAAAGTTTGCGCTTTTTTCGCTGCAGGGCAATTCACCTTTGGTTCGAAATTGCACATAATACACTCCCGGGTTGCACACCGGGAATCCAGGagag                                                                                                                                                  >  1:606845/1‑281 (MQ=255)
                                                                                                                                         ccggATTCAGGGGGGTGTAAAAGGTAAAGCGTTAAGTAAGGGGGGGGTTTGGCCGCCTAAAAGCCGGCTTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGGGGGTTGGGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTc                                                                                                                                              <  2:1192749/279‑1 (MQ=255)
                                                                                                                                         ccTGATTGAGGTGATTGTAAATGGTAAAGCGTTAAGTAAGGTGGGAGTTTGGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCGGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTc                                                                                                                                              <  2:1714177/282‑1 (MQ=255)
                                                                                                                                            gATGCAGGTGAGTGGAAATGGTAAACAGTTAAGGAAGGGGGGGTTTGTGCCGCCTAAAAGCAAGCGTCGGACAACCTGTGGTCAGGCAATCGCACGGGGTCGTGAGGCCTGGCGGGCGATAAATGGTTACGGGGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATg                                                                                                                                           <  2:1922164/282‑1 (MQ=255)
                                                                                                                                              ttCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGGGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTGTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGta                                                                                                                                         <  1:1003979/282‑1 (MQ=255)
                                                                                                                                                   gTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGgag                                                                                                                                                    >  1:1189736/1‑267 (MQ=255)
                                                                                                                                                        tgtAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGAAAAGAAGGAGAAAAATCTCCCCTGAAAAAGGTTCGGCGAACGGTTGCGTTTTTTTTGCCGCCGGTCAAATCACTTTTGGTCAGAACTCGCACATAATACTCCCCCGGTTTGCACACCGGGAATCCAGGAGCGTTCATTTACTCCCCCttc                                                                                                                               >  1:1510019/1‑282 (MQ=255)
                                                                                                                                                        tgtAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGAAAAAGACGAGCACAATCTGCCCTGAAACAGGGTCGGAAAACGTTTACGTTTTTTTTGCCCCAGGCAAAGTCCCTTTTGTCCCGGCTTCCTACAATATACTCCCCCGGCTTTCAATCCCGAGACCCAGGAGACTCCACTTCATACCCCttc                                                                                                                               >  2:373623/1‑282 (MQ=255)
                                                                                                                                                          tAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGcaca                                                                                                                                                                                          <  2:483194/223‑1 (MQ=255)
                                                                                                                                                                 tAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTCCCCCTTCGTTCGTaaa                                                                                                                      >  1:1409222/1‑282 (MQ=255)
                                                                                                                                                                    aGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACTAGCTGGGTTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGcc                                                                                                                   <  1:373623/282‑1 (MQ=255)
                                                                                                                                                                             tAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTAcccc                                                                                                                                  <  1:1183520/258‑1 (MQ=255)
                                                                                                                                                                             tAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTaa                                                                                                                       <  1:778567/269‑1 (MQ=255)
                                                                                                                                                                              agtgggtggtgtgtcCCGCCAAAAAGCCAGCGTCAGACAATGTGGATTCTGGCCATCGTGTCGTGTCGTGTCGCCTGTCGGGCGATAAATTATTACGTCGGGTTGAGTGCAAAGAAGGACCACAATCTGCCCTGAAACCGGTTTGGATAACGTTTGCGTTTTTTTAGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCACACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGc                                                                                                         <  2:606845/271‑1 (MQ=255)
                                                                                                                                                                                tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTTCAAATAAGGAGCAAAATTTGCCCTGAAAAAGGTTCGGAAAACGTTTGTGTTTTTTTTGCCGCAGGTCAATTCCCTTTTTGTCCGAACTCGCCAATAATACGCCCCCGGTTTGTACACCGGGAATCCAGGGGAGTTCATGTACTACCCCTTCGTTCGTAAAGGCCTTTTCCAGCTc                                                                                                       >  1:1215687/1‑282 (MQ=255)
                                                                                                                                                                                tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTTAAACAGGGTCGGAAAACGTTTGCGGTTTTTTTTCCGCAGGGCAATTCCCTTTTGGTCCGCACCCGGACCTAATACGCCCCCGGTTTGCACACCGGGAATACAGGGGAGTTCCTGTAATTCCCCTTCGGTCGTAAAGCCCTTTTcccctgc                                                                                                       >  2:778567/1‑277 (MQ=255)
                                                                                                                                                                                tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCgg                                                                                                                                                               >  1:1997242/1‑227 (MQ=255)
                                                                                                                                                                                tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCgg                                                                                                                                                               <  2:1997242/227‑1 (MQ=255)
                                                                                                                                                                                tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATc                                                                                                                                                          >  1:1169904/1‑232 (MQ=255)
                                                                                                                                                                                tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGACCTGAAACAGGTTCGGACAACGTTTGCGTTTTTTTTGCCGCAGGTTAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACAACGGGAATCCAGGAGAGGTCATGTACTAGCCCTTCGTTCGTAAAGCCCTTTTCCAGCTc                                                                                                       >  1:159473/1‑282 (MQ=255)
                                                                                                                                                                                tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGAAAAATCTGCCCTGAAACAGGTTCGGAAAACGGTTGCGTTTTTTTTGACGCAGGGAAATTCCCTTTTTGTCCGAACTCGCACATAATACCCCTCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGc                                                                                                                    >  1:226794/1‑269 (MQ=255)
                                                                                                                                                                                  tgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGTACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTTAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGCAAACGTTTGCGTTTTTTTTTCCGCAGGTCAATTCCCTTTTGTTCCGAACTCGCACATAATACGCCCCCCGTTTGCACCCCGGGGATCCAGGAGAGTTCATg                                                                                                                                           >  1:1847754/1‑244 (MQ=255)
                                                                                                                                                                                       gTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTTGGTGCAAAGAAGGAGCAAAAACTGCCATGAAACAGGTTCGGAAAACGTTTTGGTTTTTTTTTCTCCAGGTCAATTCCCTTTTTGTCCGAAACCGCACATAAAAAGCCCCCCGTTTGCCCACCGGGACACCAGGAGAGTATTTGTACTACCCCCTCCTTTCTAAAACCCTTTTCCACCACGactct                                                                                                 >  2:1528465/1‑277 (MQ=255)
                                                                                                                                                                                        ttGTGCCACCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGTCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATGTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCaga                                                                                               <  1:966997/282‑1 (MQ=255)
                                                                                                                                                                                          gtgCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGgag                                                                                                                                                    <  2:1306168/228‑1 (MQ=255)
                                                                                                                                                                                          gtgCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATATGCCCTGAAACAGGTTCGGAAAACGTTTTCGTTTTTTTTGCCGCAGGTCAAATCCCTTTTGGTCCGAACTCGCACATACAACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTGTCGTGACTACCCCCTCTCTCGGTACACCCTTTTCCAGCACGCACCagac                                                                                              >  2:1534261/1‑280 (MQ=255)
                                                                                                                                                                                              cgccTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAgcgcg                                                                                          <  1:1534261/281‑1 (MQ=255)
                                                                                                                                                                                               gccTAANGGCCTGCGTCAGACAAGCTGTGTTCAGGCAATCGCACCGTGTCATGTCGCCTAGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATGCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCt                                                                                        <  2:1828623/282‑1 (MQ=255)
                                                                                                                                                                                                         cAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTa                                                                              <  1:778534/282‑1 (MQ=255)
                                                                                                                                                                                                                          gtgtCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTa                                                                <  1:751550/279‑1 (MQ=255)
                                                                                                                                                                                                                          gtgtCAGGCAATCGCACCGGGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGc                                                             <  1:677719/282‑1 (MQ=255)
                                                                                                                                                                                                                          gtgtCAGGCAAGCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTGTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACg                                                              <  1:1657291/281‑1 (MQ=255)
                                                                                                                                                                                                                          gggtCAGGCAATGGTACCGTGTCATGTCGCCGTGCGGGCGATAAATGATTACGGCGGGTTTAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACg                                                              <  2:647958/279‑1 (MQ=255)
                                                                                                                                                                                                                           tgtCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGcc                                                            <  1:1961089/282‑1 (MQ=255)
                                                                                                                                                                                                                                ggCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGAGGGCTGGGTGCTAAGAACGACCCACATCTGTCCTCAGACCGGTTCGGAAAACGGTTGGGGTTTTCTTGCCGCAGTTAAATTCCCTTTTGGTCCGACCTCCGACAAAATACCACCCACGTTTGCACCCCGAGAATCCATGATAGTTCAATTTCTTACCCTTCGGTCGGAAATCTCTTTTCCTGTCCGACCCAAACGCCCCTCCGGGCTTTACTATTCCGCAATTAcccactgtt                                                       >  2:1094598/1‑274 (MQ=255)
                                                                                                                                                                                                                                          ccGGGTCATGTGGGCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATCCGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACg                                              <  2:221317/281‑1 (MQ=255)
                                                                                                                                                                                                                                             tgtCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTTCCGCAGGTCAATTCCCTTTTTGGCAGAACTCGCACCTAATACGCCCCCGGTTTGCACACCGGGGAACCAGGGGGGGTCATGTAATACCCCCTCGTTCGTAAAGACCTTTTCCAGCTCGCACCCGAACCCGCCCATGAGTTTTCTTTTCAGCAATTCTGCCGTATTACAGGGAAGCCGt                                          >  2:1147466/1‑282 (MQ=255)
                                                                                                                                                                                                                                                 aagTCGCCGGACGGGCGATAAATGATTACGGGGGGTGGGGTGCAAAGAAGGAGCAAAAGCTACCCTGAAACAGGTTCGGAAAACGGTTGCGTTTTTTTTGCCGCAGGTCAATTCCCGTTTGGTCCTAACTCGCACTTAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAAAGTGCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGa                                      <  2:159473/280‑1 (MQ=255)
                                                                                                                                                                                                                                                    tCGCCGGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTTCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCt                                                                                        <  1:1325257/229‑1 (MQ=255)
                                                                                                                                                                                                                                                    gcGCCTGTCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAATAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAACACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGTGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTAt                                                        <  1:291747/260‑1 (MQ=255)
                                                                                                                                                                                                                                                     cgccgtgcgggcgATAAATGAATACGGCGGGTTGAGTGCAAATCAGGAGCAAAACCTGCCCTGAAACAGGTTCGACAAACGTTTGCGTTTTTTTTGCCTCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGACCCCGGTTTGCACACCGGGAATCCAGGAGATTTCATGTACTACCCCTGCGTTCTTAAAGCCCTTTTCCAGCTCGATCCAGAGCCCGCTAATGAGTGTACTTTTCAGCAATTACCCCCTACTACAGGAACGCCGTTTGAAGc                                   <  2:760907/275‑1 (MQ=255)
                                                                                                                                                                                                                                                      gCCTGTCGGGTGACAAATGAGTACGGCGGGTTAAGTGCAAAGAGAGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGAAGGTCAATTCCCTTTTGGGCCGAACTCGCACATAATACGCCCCCTGTTTGCACACCGGGAATCCATGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTGTGAAGCAc                                 <  2:2614/282‑1 (MQ=255)
                                                                                                                                                                                                                                                      gCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCttcgt                                                                                                                             >  1:724406/1‑190 (MQ=255)
                                                                                                                                                                                                                                                       ccTGGCGGGCGATAAATGATTACGGCGGGTTGTGTGCAAAGAAGGAGCCAAATCTGCCCTGAAAAAGGTTCGGAAAACGTTTGCGTTTTTTTTTCCGCAGGTCAATTCCCTTTTTGTCCGTACTCGCACATAATACGCCCCCGGTTTTCAAACCGGGAATCCAGGAGAGTTGATGTACTCACCCTTCGTTCGTAAAGCCCTTTTCCAGATCGCTCCCGAGCGCGCCCATTCGTTTACTTTTTCACAATTTCGCCCTATTTACGCGACGCCGTTTGaacaact                                >  2:1830921/1‑277 (MQ=255)
                                                                                                                                                                                                                                                       ccTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTTCACACCGGGGATCCAGGAGGGTTCATTTACTACCCCTTCGTTCGTAAAGCCCTTTTCCCACCCGATCCAGAACGCCCCCATTAGTTTACTTTTCAGCAATTTAGCCGTATTACAGGAACGCCGTTTGCAGcca                                 >  1:1133564/1‑279 (MQ=255)
                                                                                                                                                                                                                                                           gcgggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGt                             <  2:360933/281‑1 (MQ=255)
                                                                                                                                                                                                                                                            cgggcgATAAATGATTGCGTCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCTGAAAACTTTTGCGTTTTTTTTGCCGCGGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGc                           <  1:1782377/282‑1 (MQ=255)
                                                                                                                                                                                                                                                              ggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAAAGCCGTTTGAAGCACTGGTTCgg                         >  1:558216/1‑282 (MQ=255)
                                                                                                                                                                                                                                                                      aTGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTc                                                                        <  1:1175762/227‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gATTACGGCGGGTTGATTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAAGACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTg                <  1:841205/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ttACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGca                                                                                                                                                                                            <  1:1441688/109‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ggcggGTTGAGTGCAAAGAAGGTGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGGGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGa         <  1:1094598/282‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gtagggTTGAGGGCAAAGAAGGAGCAAAATCTGCCATGAAACCGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGTTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCTCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGTCAGAAAGTGCCTGCGa         <  1:529739/279‑1 (MQ=255)
                                                                                                                                                                                                                                                                                cggGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCGGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGa         <  1:1044318/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  ggtttaaGTGCAAAGAAGAAGCAAAATCTGCCCTTAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACTCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAAc      <  2:558216/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    ggttaGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCTGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATATGCCCCCGGTTTGCACACCGGGAACCCCGGAGAGTTCATGTACTACCCCTTCGTTCCTAAAGCCCCTTTCCAGCTCGCTCCACAGCGCGCCCATGAGTTTACTTTTCACCAATTTCGCTGTATTACAGGAACGCCGTTTGAAGCACTTGTTCGGCAGAAAAGGCCTggccaacc     >  2:73073/5‑274 (MQ=255)
                                                                                                                                                                                                                                                                                    gTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGGTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGaaa       >  1:324298/1‑279 (MQ=255)
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           
GCGATGCGCTCGGTATTTAAACTGTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGT  >  NC_000913/1004349‑1004907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: