New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3714533	187 (1.270)	3 (0.020)	3/454	NT	1.6%	coding (1862/2334 nt)	bisC	biotin sulfoxide reductase
	?	NC_000913	3714546 =	188 (1.300)					coding (1849/2334 nt)	bisC	biotin sulfoxide reductase
Rejected: Frequency below/above cutoff threshold.

TCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3714283‑3714533 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAA  >  NC_000913/3714546‑3714819                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               TCCCTCGTGAATGACCGCTCCGGCAAGAATTTGCCAGCGTGCGTTCCACAACCGAACAGTATCGCATTCTTGTATGCCGCGCTCCTGGGCGTCATCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCGGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCCAGGGCAATCCGGGTAACCGTAAT                                                                                                                                                                                                                                                            <  1:601411/275‑1                                                                              CGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAG                                                                                                                                                                                                                                                                                      >  1:593943/1‑172                                                                                  CTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAG                                                                                                                                                                                                                                                                                      >  1:163947/1‑168                                                                                                                                                                   CTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGG                                                                                                                      >  2:537049/1‑247                                                                                                                                                                       CTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGG                                                                                                                  <  1:537050/247‑1                                                                                                                                                                                                                                                           CCAGGGCAATCCGGTTAACCGTAATGGGCAATACGCAGTGAGAAGATTTCAATCTTGCCGGTGGCGGTTTTTAACGGAGGCGCCAGCGGATCGCTGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTCCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAA  <  2:1079574/275‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               TCCCTCGCTAATGACCGCTCCGGCAAGAATTTGCCCGCGTGCGTTCCACAACCGAACAGTATCGCCATCTTGTATGCCGCGCTCCTGGGCATCGTCAGGATGAATGGTGACAGGCTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3714283‑3714533 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCTCGCTGTCCGGGTTTTCCGGCATCTCAATTAACTGGTTGGCTTGCCAGAACTCAGCAAATGGCGGCAATTCAACCTGCTGGCTTGCCCCGCGCTGTCGGGCAACGTTATAAAACGTTTCCAGCCATTGCAGCTCACTTTTTCCTTCCGTAAA  >  NC_000913/3714546‑3714819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.