Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,334,015 A→G 58.5% K54R (AAG→AGG)  cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,334,0150AG58.5% 54.5 / 147.4 111K54R (AAG→AGG) cysBN‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base A (29/17);  new base G (40/25);  total (69/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01

Only 100 of 111 total aligned reads displayed.

TAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGCCGTCTCTAAAGGCAAT  >  NC_000913/1333734‑1334280
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                         
tAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTg                                                                                                                                                                                                                                                                    >  2:529792/1‑289 (MQ=255)
tAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTg                                                                                                                                                                                                                                                                    >  2:165660/1‑289 (MQ=255)
  aGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGAc                                                                                                                                                                                                                                                                  >  1:413244/1‑289 (MQ=255)
  aGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGAc                                                                                                                                                                                                                                                                  >  1:541964/1‑289 (MQ=255)
  aGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGAc                                                                                                                                                                                                                                                                  >  2:482334/1‑289 (MQ=255)
              atatAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGAGGAGCTAGGCATTCACATTTTTTACCGAAGCGGCAAGCACCTGACGACGGTAACGc                                                                                                                                                                                                                                                       >  2:602168/1‑288 (MQ=255)
               tataGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCca                                                                                                                                                                                                                                                     >  1:524024/1‑289 (MQ=255)
                  aGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCcagca                                                                                                                                                                                                                                                  <  2:524024/289‑1 (MQ=255)
                      gTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGc                                                                                                                                                                                                                                              >  2:692576/1‑289 (MQ=255)
                         ataGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCTAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAg                                                                                                                                                                                                                                           >  2:400500/1‑289 (MQ=255)
                                gCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGaa                                                                                                                                                                                                                                         >  2:27659/1‑284 (MQ=255)
                                gCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGaa                                                                                                                                                                                                                                         <  1:27659/284‑1 (MQ=255)
                                gCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAaataat                                                                                                                                                                                                                                    >  1:559921/1‑289 (MQ=255)
                                 cACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAaataat                                                                                                                                                                                                                                    >  1:483403/1‑288 (MQ=255)
                                      tttttATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAt                                                                                                                                                                                                                              <  1:165660/289‑1 (MQ=255)
                                                   aTCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTc                                                                                                                                                                                                                 <  2:413244/289‑1 (MQ=255)
                                                                 ccGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGa                                                                                                                                                                                                   >  1:462880/1‑289 (MQ=255)
                                                                 ccGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAACGCTCGCGAAGTCTTGTCGAAAGTCgc                                                                                                                                                                                                   >  2:707095/1‑288 (MQ=255)
                                                                 ccGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGa                                                                                                                                                                                                   >  1:152464/1‑289 (MQ=255)
                                                                        gtgtGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATa                                                                                                                                                                                            >  1:11796/1‑289 (MQ=255)
                                                                             aCGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAAt                                                                                                                                                                                        >  2:688204/1‑288 (MQ=255)
                                                                                 aaaaaCGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGtt                                                                                                                                                                                   >  2:454692/1‑289 (MQ=255)
                                                                                     aCGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg                                                                                                                                                                               <  2:11796/289‑1 (MQ=255)
                                                                                     aCGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg                                                                                                                                                                               <  2:483403/289‑1 (MQ=255)
                                                                                       gATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGga                                                                                                                                                                             <  1:602168/289‑1 (MQ=255)
                                                                                            tCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGca                                                                                                                                                                        <  1:692576/289‑1 (MQ=255)
                                                                                                   gCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg                                                                                                                                                                               >  2:721543/1‑275 (MQ=255)
                                                                                                   gCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg                                                                                                                                                                               <  1:721543/275‑1 (MQ=255)
                                                                                                    cGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTgg                                                                                                                                                                 <  2:559921/288‑1 (MQ=255)
                                                                                                     gTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGc                                                                                                                                                                <  1:707095/288‑1 (MQ=255)
                                                                                                              gATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTACCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAgg                                                                                                                                                      >  2:20031/1‑289 (MQ=255)
                                                                                                                    ttAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCAc                                                                                                                                                 <  1:454692/288‑1 (MQ=255)
                                                                                                                    ttAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCAc                                                                                                                                                 <  1:400500/288‑1 (MQ=255)
                                                                                                                        cATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCa                                                                                                                                                  <  1:573921/283‑1 (MQ=255)
                                                                                                                        cATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCa                                                                                                                                                  >  2:573921/1‑283 (MQ=255)
                                                                                                                             aaTTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCCGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTTTCGAAAGTCGATGCCATAAAATCGGTTGCCGGGGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCg                                                                                                                                        >  1:410031/1‑288 (MQ=255)
                                                                                                                             aaTTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTgg                                                                                                                                                                 <  1:635381/263‑1 (MQ=255)
                                                                                                                             aaTTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTgg                                                                                                                                                                 >  2:635381/1‑263 (MQ=255)
                                                                                                                             aaTTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGagag                                                                                                                                                                          <  2:180902/254‑1 (MQ=255)
                                                                                                                             aaTTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGagag                                                                                                                                                                          >  1:180902/1‑254 (MQ=255)
                                                                                                                             aaTTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCg                                                                                                                                        >  1:334122/1‑288 (MQ=255)
                                                                                                                                    acaacTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACg                                                                                                                                <  1:529792/289‑1 (MQ=255)
                                                                                                                                    acaacTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACg                                                                                                                                <  1:20031/289‑1 (MQ=255)
                                                                                                                                        cTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCAt                                                                                                                             >  1:355247/1‑288 (MQ=255)
                                                                                                                                        cTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTACCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCAt                                                                                                                             >  1:613027/1‑288 (MQ=255)
                                                                                                                                         ttCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATa                                                                                                                            >  1:552569/1‑288 (MQ=255)
                                                                                                                                                     ttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGc                                                                                                                               >  1:244731/1‑273 (MQ=255)
                                                                                                                                                     ttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGc                                                                                                                               <  2:244731/273‑1 (MQ=255)
                                                                                                                                                     ttgAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCt                                                                                                                >  1:236170/1‑288 (MQ=255)
                                                                                                                                                       gAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACg                                                                                                             <  2:541964/289‑1 (MQ=255)
                                                                                                                                                        aGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGc                                                                                                            >  1:46837/1‑289 (MQ=255)
                                                                                                                                                          gttgtCAAGCATAACCTGAATGTCTCATCAACAGCGGAAGGACGTTACACAGCACAACCCCGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCa                                                                                                           <  2:462880/285‑1 (MQ=255)
                                                                                                                                                          gtggtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATAACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGcca                                                                                                                                     <  2:423993/262‑1 (MQ=255)
                                                                                                                                                             gtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTa                                                                                                        >  1:198861/1‑288 (MQ=255)
                                                                                                                                                             gtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTa                                                                                                        >  1:374944/1‑288 (MQ=255)
                                                                                                                                                             gtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTatat                                                                                                                                          >  2:280268/1‑254 (MQ=255)
                                                                                                                                                             gtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTatat                                                                                                                                          <  1:280268/254‑1 (MQ=255)
                                                                                                                                                             gtCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACg                                                                                                                  >  2:227113/1‑278 (MQ=255)
                                                                                                                                                              tCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTAc                                                                                                       <  1:688204/288‑1 (MQ=255)
                                                                                                                                                                        ccTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGtcatc                                                                                            >  1:162138/1‑289 (MQ=255)
                                                                                                                                                                            aaTGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAg                                                                                        >  2:70418/1‑289 (MQ=255)
                                                                                                                                                                            aaTGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAg                                                                                        >  2:656350/1‑289 (MQ=255)
                                                                                                                                                                            aaTGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCAACACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAg                                                                                        >  1:659402/1‑289 (MQ=255)
                                                                                                                                                                            aaTGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAACCGTCATCAAAg                                                                                        >  1:265941/1‑289 (MQ=255)
                                                                                                                                                                            aaTGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAg                                                                                        >  2:242883/1‑289 (MQ=255)
                                                                                                                                                                                 ctcATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGGTCACTGTATATCGCCAACACGCATACCCAGGCACGATACGCATTACCAAACGTCATCAAACGCttt                                                                                   >  2:403703/1‑289 (MQ=255)
                                                                                                                                                                                    atcaACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCCAAGGCCTTAtt                                                                                >  2:326776/1‑289 (MQ=255)
                                                                                                                                                                                          aGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCa                                                                                                           <  2:340649/256‑1 (MQ=255)
                                                                                                                                                                                          aGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCa                                                                                                           >  1:340649/1‑256 (MQ=255)
                                                                                                                                                                                            cGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTa                                                                        >  1:597024/1‑289 (MQ=255)
                                                                                                                                                                                             ggAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCg                                                                           <  1:482334/285‑1 (MQ=255)
                                                                                                                                                                                             ggAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCg                                                                           <  2:334122/285‑1 (MQ=255)
                                                                                                                                                                                              gAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCCGGCACGCGACGCATTACCAAACGTCCTCAAAGGCTTTATTGAGCGTTAt                                                                       >  2:661222/1‑288 (MQ=255)
                                                                                                                                                                                                   aCTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCaa                                                                                          <  1:416732/264‑1 (MQ=255)
                                                                                                                                                                                                   aCTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTcg                                                                  <  2:236170/288‑1 (MQ=255)
                                                                                                                                                                                                             tCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGc                                                       >  2:241190/1‑289 (MQ=255)
                                                                                                                                                                                                             tCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATCAAGGTTCACTGTATATAGCCACCACGCATACCAAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGc                                                       >  1:497570/1‑289 (MQ=255)
                                                                                                                                                                                                              cacaACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGcaca                                                                                                                                                                      <  2:298401/177‑1 (MQ=255)
                                                                                                                                                                                                              cacaACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGcaca                                                                                                                                                                      >  1:298401/1‑177 (MQ=255)
                                                                                                                                                                                                                   cccGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTcgcg                                                                                                                                                                                                                      <  2:542430/124‑1 (MQ=255)
                                                                                                                                                                                                                   cccGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGc                                                 >  1:566311/1‑289 (MQ=255)
                                                                                                                                                                                                                      gggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCttt                                                         <  2:396026/278‑1 (MQ=255)
                                                                                                                                                                                                                      gggATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCttt                                                         >  1:396026/1‑278 (MQ=255)
                                                                                                                                                                                                                        gATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCAACTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGACGTCCTGTCGAACGTCGATGCCATAAAATCGGTTGCCGGAGAGCACGCCTGGCCGGTGAAAGGTTTACTGTATATCGCCACCACGCATACCCCGGCCCGCTACGCATTACCAACCGTCATCCAAGGCTATACTACGCGTTATCCTCTCGGTTCTTTGccttttcaactg                                            >  2:745132/1‑278 (MQ=255)
                                                                                                                                                                                                                             gTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGga                                                                                                                                                                             <  2:202966/155‑1 (MQ=255)
                                                                                                                                                                                                                             gTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGga                                                                                                                                                                             >  1:202966/1‑155 (MQ=255)
                                                                                                                                                                                                                               aaaCAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCg                                      >  1:210887/1‑288 (MQ=255)
                                                                                                                                                                                                                                 aCAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCg                                   >  1:280037/1‑289 (MQ=255)
                                                                                                                                                                                                                                 aCAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCg                                   <  1:70418/289‑1 (MQ=255)
                                                                                                                                                                                                                                  cAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGa                                  <  2:198861/289‑1 (MQ=255)
                                                                                                                                                                                                                                     gTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAAAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCAACCAAGCATACCCAGGCACGCTACGCATTACCAAACGACATCAAAGGCTTTATTGAACGTTATCCTCGCGTTTCTTTGCATAAGAACCAGGGCTCGACgcaac                               >  1:627628/1‑285 (MQ=255)
                                                                                                                                                                                                                                      tCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGAcaca                              >  1:258089/1‑289 (MQ=255)
                                                                                                                                                                                                                                               tGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTCAGCGTTAACCTAGCGTTTCTTTCGATATTCACCAGGGCTCGCTTACCACAATTGCTg                      >  2:232917/1‑288 (MQ=255)
                                                                                                                                                                                                                                                   aGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGc                  <  2:566311/288‑1 (MQ=255)
                                                                                                                                                                                                                                                    gACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGcc                 <  1:241999/288‑1 (MQ=255)
                                                                                                                                                                                                                                                      cgggCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGCCGtc              <  1:158541/286‑1 (MQ=255)
                                                                                                                                                                                                                                                      cGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAGGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGCCg                <  2:552569/287‑1 (MQ=255)
                                                                                                                                                                                                                                                         gCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGCCGTctct           >  2:38678/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                   cAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGCCGTCTCTAAAGGCAAt  >  1:621474/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   cAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGCCGTCTCTAAAGGCAAt  >  1:542441/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                         
TAAGGCTATAAATGATATAGTGGTTATAGTTAGCACCTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAAGTGGATGGTTTAACATGAAATTACAACAACTTCGCTATATTGTTGAGGTGGTCAATCATAACCTGAATGTCTCATCAACAGCGGAAGGACTTTACACATCACAACCCGGGATCAGTAAACAAGTCAGAATGCTGGAAGACGAGCTAGGCATTCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAAAGGTTCACTGTATATCGCCACCACGCATACCCAGGCACGCTACGCATTACCAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCCGACACAAATTGCTGATGCCGTCTCTAAAGGCAAT  >  NC_000913/1333734‑1334280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: