Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,209,209 Δ1 bp 7.1% coding (1186/1464 nt) ttdT → L‑tartrate/succinate antiporte

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,209,2090A. 7.1% 189.8 / 5.3 56coding (1186/1464 nt)ttdTL‑tartrate/succinate antiporte
Reads supporting (aligned to +/- strand):  ref base A (24/28);  new base . (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTA  >  NC_000913/3208923‑3209486
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                     
gATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAgc                                                                                                                                                                                                                                                                                     >  1:306008/1‑289 (MQ=255)
    ttCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCa                                                                                                                                                                                                                                                                                 <  2:306008/289‑1 (MQ=255)
                                                                ctTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACg                                                                                                                                                                                                                                                                               <  1:129107/231‑1 (MQ=255)
                                                                ctTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACg                                                                                                                                                                                                                                                                               >  2:129107/1‑231 (MQ=255)
                                                                      cgcATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACACTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCAAGCGCCACGGCGTATAACTCCGCTCCCGCACCGATCATGATTGCTGCTGCGTCGGGGATGCCGGAAATCCCGc                                                                                                                                                                                                               >  2:489368/1‑289 (MQ=255)
                                                                           tATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCg                                                                                                                                                                                                          >  2:157393/1‑289 (MQ=255)
                                                                                  tGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGgtgt                                                                                                                                                                                                      <  1:442428/286‑1 (MQ=255)
                                                                                   gggACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGCTGATTGTTGCTGCGCTGGCTATGCCGGCAATCCCGCTGCCGGTGTTcgc                                                                                                                                                                                                  >  2:408706/1‑286 (MQ=255)
                                                                                   gggACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCCCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCGCGCCACGGCTTATACCTCCCCTCTCGCCCCGATGATGCTTTCTGCTTCGCTGGCTATGCCTGAAACCCCGCTGCCGGTGTTccg                                                                                                                                                                                                  >  2:157152/1‑286 (MQ=255)
                                                                                         acATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACg                                                                                                                                                                                                                                                                               >  1:101158/1‑206 (MQ=255)
                                                                                         acATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACg                                                                                                                                                                                                                                                                               <  2:101158/206‑1 (MQ=255)
                                                                                            ttGTCAGTAATAAATCGGCGTGGAACGTTTTCTTCTGGATGGCCTCGCGTATCACCCTCGCCACCGGACTCAACAACACCGGTGTTATTAGCTGGTTTGGCAAACTGTTAGCAGGATGCTTAAGAGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGAGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGAtggt                                                                                                                                                                                         <  2:174553/289‑1 (MQ=255)
                                                                                              gTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGCAATCCCGCTGCTGGTGTTCTGCCCGAcggttg                                                                                                                                                                                       >  2:306671/1‑289 (MQ=255)
                                                                                                    aataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGc                                                                                                                                                                                   <  1:157393/287‑1 (MQ=255)
                                                                                                       aagggggggTGGAACGTTTTCCTCTGGCTTGCCTCGCTTATCACCCTCGCCACCGGACGCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGGTGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCaa                                                                                                                                                                              <  2:170413/281‑1 (MQ=255)
                                                                                                         aGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgctg                                                                                                                                                                                                                                          >  1:303867/1‑227 (MQ=255)
                                                                                                         aGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgctg                                                                                                                                                                                                                                          <  2:303867/227‑1 (MQ=255)
                                                                                                             gcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCa                                                                                                                                                                                                                                                                                 >  1:312439/1‑184 (MQ=255)
                                                                                                             gcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCa                                                                                                                                                                                                                                                                                 <  2:312439/184‑1 (MQ=255)
                                                                                                             gcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTgg                                                                                                                                                                          >  1:407167/1‑287 (MQ=255)
                                                                                                                tGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTgg                                                                                                                                                                     >  2:15690/1‑289 (MQ=255)
                                                                                                                          tcttctGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCt                                                                                                                                                           >  1:355898/1‑289 (MQ=255)
                                                                                                                                 gctggcCTCTCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTacac                                                                                                                                                      <  2:148923/287‑1 (MQ=255)
                                                                                                                                 gctggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTacac                                                                                                                                                      >  1:148923/1‑287 (MQ=255)
                                                                                                                                      ccTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACg                                                                                                                                                <  1:15690/288‑1 (MQ=255)
                                                                                                                                                    cTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGt                                                                                                                                  >  1:151646/1‑288 (MQ=255)
                                                                                                                                                    cTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCCGAAATCCCGCTGCCGGTGTTCTTCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTCACACACTACTCCACCTGTCCCAGt                                                                                                                                  >  2:465819/1‑288 (MQ=255)
                                                                                                                                                               aCTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTtacta                                                                                                                       <  1:366952/288‑1 (MQ=255)
                                                                                                                                                                 tCAACAACTCCCGTTTGATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACgg                                                                                                                    <  2:407167/289‑1 (MQ=255)
                                                                                                                                                                   aacaacACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgat                                                                                                                                                                                                                                                  >  2:289073/1‑161 (MQ=255)
                                                                                                                                                                   aacaacACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgat                                                                                                                                                                                                                                                  <  1:289073/161‑1 (MQ=255)
                                                                                                                                                                     caacaCCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGt                                                                                                                <  2:355898/289‑1 (MQ=255)
                                                                                                                                                                        cacCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCTAACGATGGTGATGGTGGCGTTGATTGCGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGACATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGTCCCAGTCCGATTTACTACGGCAGTGGt                                                                                                             >  1:59961/1‑289 (MQ=255)
                                                                                                                                                                             gTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCt                                                                                                        >  2:323151/1‑289 (MQ=255)
                                                                                                                                                                                        tGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCTCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAAcggcg                                                                                              >  2:4419/1‑288 (MQ=255)
                                                                                                                                                                                         ggTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGa                                                                                            <  1:489368/289‑1 (MQ=255)
                                                                                                                                                                                          gTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGAt                                                                                           >  2:170164/1‑289 (MQ=255)
                                                                                                                                                                                                            aggcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGc                                                                          <  1:170164/288‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGccc                                                                                                                                      >  1:94870/1‑203 (MQ=255)
                                                                                                                                                                                                                                     aCGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGccc                                                                                                                                      <  2:94870/203‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTAt                                                >  1:115389/1‑289 (MQ=255)
                                                                                                                                                                                                                                        atggtgatggtgGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTg                                              <  2:151646/288‑1 (MQ=255)
                                                                                                                                                                                                                                        atggtgatggtgGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTg                                              <  1:4419/288‑1 (MQ=255)
                                                                                                                                                                                                                                                 gtggCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAg                                                                                                                                                                 >  2:275632/1‑164 (MQ=255)
                                                                                                                                                                                                                                                 gtggCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAg                                                                                                                                                                 <  1:275632/164‑1 (MQ=255)
                                                                                                                                                                                                                                                         gATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTa                            <  1:306671/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         gATTATGGTGGTTGTTCTACTGCGCTACTTTTACGCCAGCGCCACGGCGTATACCGCCGCTCTCGCACCGATGATGAGTGCTGTTGCGCTGGCGAAGCCGGCAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGAGCGGCGATTGGTCTGGTGAGCATTCTTACTCCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTa                            <  2:59961/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gtggtgTTTTATCTACTGCGCTACTTTTTCGCCGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACtgtg                       <  1:408706/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gtggtgTTTTATCTACTGCGCTACTTTTTCGCCGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACtgtg                       <  1:157152/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tgtTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGAt                    <  1:323151/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCgtg             >  1:275539/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          tACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCgtggt           <  1:391589/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            catcacTACTTTTTCGCCAGGGCTACGACGTTTACCGCCGTCCCCGCACCGTGAATGATTGCTCATTTGCTGGCGATGCCGGAAATCCCGCGCCCGGGTTTCTGCCGGATGGTTGGTGCGCCAATTGGTCTGGGTAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGTTTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATAGTCCTCGTATTGCTGTTGATTACCGGCTTACTGTGGATGCCCGTGgtgt         <  2:97237/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGgtgt         <  2:115389/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cgcTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGCTGATTGCTGCTGCGCTGGCGATGCCGGACATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGACCATTCTTACACCATCCGCCAACGGCCCCAGCCCGATTTACTACGCCAGTGGTTATCTGCCCACCGCGGATTACTGCCTACTGGGGGCGCTTTTTGGGCTCATATTCCTCGTTTTGCTGTTCATTACCGGCTTACTTCGGTTGCCCGTGGttttct      >  2:222832/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                   aCTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTa  <  1:130791/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                     
GATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGCCTGATGGTTGGTGCGGCAATTGGTCTGGGGAGCATTCTTACACCATACGCCACCGGCCCCAGTCCGATTTACTACGGCAGTGGTTATCTGCCAACGGCGGATTACTGGCGACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTA  >  NC_000913/3208923‑3209486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: