Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,450,334 G→T 8.5% P86Q (CCG→CAG)  yfcP ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,450,3340GT 8.5% 162.6 / 5.9 47P86Q (CCG→CAG) yfcPputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTATTCATTGATAATCCACCCGTAATGTTGCGTA  >  NC_000913/2450069‑2450616
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                          
cGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTa                                                                                                                                                                                                                                                                     >  2:412817/1‑289 (MQ=255)
  cAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTaaa                                                                                                                                                                                                                                                                   >  2:490457/1‑289 (MQ=255)
  cAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTaaa                                                                                                                                                                                                                                                                   <  1:367347/289‑1 (MQ=255)
               ggTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACt                                                                                                                                                                                                                                                               <  1:1749/280‑1 (MQ=255)
               ggTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACt                                                                                                                                                                                                                                                               >  2:1749/1‑280 (MQ=255)
                       cACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGa                                                                                                                                                                                                                                              <  1:318185/289‑1 (MQ=255)
                        aCCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATTCCAATTCCCACTCCCTGAGCGGCTGATGACGTATCCAGTGCCACAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGCGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTCCAATCTTTCAGCTCAAAGACTACGGGTACACGAGCggt                                                                                                                                                                                                                                              >  2:120318/1‑287 (MQ=255)
                                 cacaTCACGGCCGCTTTTCGCCGGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCa                                                                                                                                                                                                                                    <  1:39884/289‑1 (MQ=255)
                                      cACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGATCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGt                                                                                                                                                                                                                                >  2:37028/1‑288 (MQ=255)
                                         ggCCGCTTTTCGCCATGAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCaa                                                                                                                                                                                                                            <  1:120318/289‑1 (MQ=255)
                                                                             tatCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGc                                                                                                                                                                                        <  1:412817/289‑1 (MQ=255)
                                                                                cGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCCTGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTCCAGCTTAAAGACTACGGGTACACGAGCGGACAGGCCTGCACCGTTCAAATCCTGCCTACTGACGCTGGGCAAGCGCACCTCTCCCAg                                                                                                                                                                                     >  2:299124/1‑289 (MQ=255)
                                                                                      tGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCttt                                                                                                                                                                                                                                                                            <  1:335045/196‑1 (MQ=255)
                                                                                      tGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCttt                                                                                                                                                                                                                                                                            >  2:335045/1‑196 (MQ=255)
                                                                                         cATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  1:490457/288‑1 (MQ=255)
                                                                                                 aGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccacc                                                                                                                                                                     >  1:487935/1‑288 (MQ=255)
                                                                                                        aGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCa                                                                                                                                                                           <  2:433889/275‑1 (MQ=255)
                                                                                                        aGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCa                                                                                                                                                                           >  1:433889/1‑275 (MQ=255)
                                                                                                                         ttgttgATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             >  1:202341/1‑256 (MQ=255)
                                                                                                                         ttgttgATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  2:202341/256‑1 (MQ=255)
                                                                                                                                 ggCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    >  2:224702/1‑289 (MQ=255)
                                                                                                                                      ccTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    >  1:312372/1‑284 (MQ=255)
                                                                                                                                      ccTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    <  2:312372/284‑1 (MQ=255)
                                                                                                                                             tCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGa                                                                                                                        <  2:487935/289‑1 (MQ=255)
                                                                                                                                                 tCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    >  2:274159/1‑273 (MQ=255)
                                                                                                                                                 tCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCg                                                                                                                                    <  1:274159/273‑1 (MQ=255)
                                                                                                                                                                               tGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  1:262683/202‑1 (MQ=255)
                                                                                                                                                                               tGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             >  2:262683/1‑202 (MQ=255)
                                                                                                                                                                               tGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCAtt                                                                                       >  1:35245/1‑288 (MQ=255)
                                                                                                                                                                                       tgatgaCGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATc                                                                              >  2:28747/1‑289 (MQ=255)
                                                                                                                                                                                          tgaCGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGc                                                                           <  1:224702/289‑1 (MQ=255)
                                                                                                                                                                                                aTCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             <  1:343271/185‑1 (MQ=255)
                                                                                                                                                                                                aTCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGc                                                                                                                                                                             >  2:343271/1‑185 (MQ=255)
                                                                                                                                                                                                   cAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCTGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAAc                                                                  <  1:37028/289‑1 (MQ=255)
                                                                                                                                                                                                      tGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAgcgc                                                                                                                                                             >  2:225465/1‑195 (MQ=255)
                                                                                                                                                                                                      tGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAgcgc                                                                                                                                                             <  1:225465/195‑1 (MQ=255)
                                                                                                                                                                                                            aaaaCCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACg                                                            >  2:325175/1‑286 (MQ=255)
                                                                                                                                                                                                             aaaCCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGt                                                           <  1:325175/286‑1 (MQ=255)
                                                                                                                                                                                                                          cGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAAt                                           <  2:35245/289‑1 (MQ=255)
                                                                                                                                                                                                                             tGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCAt                                        >  1:54407/1‑289 (MQ=255)
                                                                                                                                                                                                                              gTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGCCTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCCCTGCCAGATATGGGCCATCCACCACCAGCGCCCAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCCAATAACGTGCTCCTCATTGCCAATACCAGCCCCGCTAAAAGGCTGTAACCACGCGACTGCTTCAtt                                       >  2:290665/1‑289 (MQ=255)
                                                                                                                                                                                                                                 ttctACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGAt                                    <  1:28747/289‑1 (MQ=255)
                                                                                                                                                                                                                                    tACCCCCTGGGGCGTAACTTTGACGTTGTATCCCGCCGGCCCTTTGCAATCGTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTa                                 <  1:290665/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              tGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccaccacca                                                                                                                                                                 <  1:448084/135‑1 (MQ=255)
                                                                                                                                                                                                                                                              tGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATccaccacca                                                                                                                                                                 >  2:448084/1‑135 (MQ=255)
                                                                                                                                                                                                                                                               gTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTATTCATTGATAATCCACCCGTAATGtt       >  1:345263/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   cccGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTCTTCATTGATAATCAACCCGTAATGTTGCGtc  >  2:469266/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                          
CGCAGTTAAAGAGGCGGTAAATTCACCGATCGTCACATCACGGCCGCTTTTCGCCTGTAGCCAGGCTCTGAAATTGATATCGTTATTGCCATTGGTTAGCGCAAAGGTCGCCCCATTTGTATTGTTGATGGCAACCTGCATTCCGTCGGTTTTTTCCATGCCAATTCCCACTCCCTGAGCGGTTGATGACGTATCCAGTGCCAGAAAACCGGGTTGTTCGCTGTCTTCTACCCCCGTGAGCGTGACTTTTACGTTGTATCCCGCCGGCCCTTTGCAATCTTTCAGCTTAAAGACTACGGGTACACGAGCGGACTGGCCTGCAACGTTCAAATCCTGACGACTGACCGTGGGAAAGCGCACCTCTGCCAGATATTGGCCATCCACCACCAGCGCACAGGATTTACTTAATAAATTACCGGAGAAATGCAGATTATTATCTGCCGCGAATAACGTGCTACTCATTGCCAATATCAGCCCCGCTAACAGGACGTAACCACCGGACTGAATCATTGATTTATTCATTGATAATCCACCCGTAATGTTGCGTA  >  NC_000913/2450069‑2450616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: