Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,773,070 A→G 8.5% pseudogene (113/117 nt) yfjV ← pseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,773,0700AG 8.5% 147.3 / 4.7 47pseudogene (113/117 nt)yfjVpseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein
Reads supporting (aligned to +/- strand):  ref base A (20/23);  new base G (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

CCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAG  >  NC_000913/2772782‑2773349
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                       
ccTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATACTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCa                                                                                                                                                                                                                                                                                         >  1:150884/1‑289 (MQ=255)
ccTGAAGGTTGCCAGAGCCTGAATCGAACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCa                                                                                                                                                                                                                                                                                         <  2:176209/289‑1 (MQ=255)
              gATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGAt                                                                                                                                                                                                                                                                                  >  1:8561/1‑282 (MQ=255)
              gATCCTTGATCGCACTGGCAAGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGAt                                                                                                                                                                                                                                                                                  <  2:8561/282‑1 (MQ=255)
                         gCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAAt                                                                                                                                                                                                                                                                <  1:159014/289‑1 (MQ=255)
                           aCTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCg                                                                                                                                                                                                                                                              <  1:68672/289‑1 (MQ=255)
                                                 gAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCgcggcg                                                                                                                                                                                                                                          <  1:213243/287‑1 (MQ=255)
                                                 gAAACTTAATACGTGGCCGGAAAGATGCTGCGAAGGAACGGATACGGCATAAGCACCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACGGCGAAAAGCTCAGACCGAAGAAGGCCGCCGGGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCgcggcg                                                                                                                                                                                                                                          <  2:165997/287‑1 (MQ=255)
                                                               tGCCGGAATAACTCTGCGAAAGAAGGGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaac                                                                                                                                                                                                                           <  2:292873/288‑1 (MQ=255)
                                                               tGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaaca                                                                                                                                                                                                                          >  1:292873/1‑289 (MQ=255)
                                                                gCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAACGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCTAGCACCATCGGCGACAGAATCAGAGCGGCGCCTTCATTGGTAtacaa                                                                                                                                                                                                                         >  2:81810/1‑289 (MQ=255)
                                                                           tctGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAg                                                                                                                                                                                                              <  1:294790/289‑1 (MQ=255)
                                                                                              aGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaac                                                                                                                                                                                                                           >  2:403518/1‑257 (MQ=255)
                                                                                              aGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaac                                                                                                                                                                                                                           <  1:403518/257‑1 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATACATCCTGCAGCCATGACAAAGGCCAGTGTCGTTCCCTGGCTGAACCCAGTGCGATCAGCATAGCAATCAAAAAGCGCGCCAGGTACCGCGGGCGCCGTAATTGGCAAACAACGCGTCACAAGGGACACCGAGACAGGCTTTCCAgggctacagc                                                                                                                                                                              >  2:191779/1‑281 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAAt                                                                                                                                                                                                                                                                <  1:139745/208‑1 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAAt                                                                                                                                                                                                                                                                >  2:139745/1‑208 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGACCAGCATCGCAATCACAATCGGCCTCAGGATCAGCTCGGCGCCGTCATTTGCAAACAACGCATCAACCGCCGCACCACGCAAGACtttaccggcaaaaa                                                                                                                                                                                >  2:218343/1‑277 (MQ=255)
                                                                                                             gCCGCAAGCGCGGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAgcg                                                                                                                                                                             <  2:377072/288‑1 (MQ=255)
                                                                                                               cGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGACAGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGct                                                                                                                                                                           <  2:48969/288‑1 (MQ=255)
                                                                                                                      gCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGctc                                                                                                                                                                          <  1:133518/282‑1 (MQ=255)
                                                                                                                                ccACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCCAGACTTTCCAGGTGAACAGCGACCTAGGTAGGAAcctgtc                                                                                                                                                          >  2:133518/1‑283 (MQ=255)
                                                                                                                                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACCGCGCTCtaggt                                                                                                                                                                     >  2:277540/1‑267 (MQ=255)
                                                                                                                                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCtaggt                                                                                                                                                                     <  1:277540/267‑1 (MQ=255)
                                                                                                                                                ggAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCATGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCCAGGTAGGAACTTTGTCGTTGATAGAGTCTTCa                                                                                                                                         <  1:439766/289‑1 (MQ=255)
                                                                                                                                                       tACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCAt                                                                                                                                    <  1:496612/287‑1 (MQ=255)
                                                                                                                                                       tACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCAt                                                                                                                                    >  2:496612/1‑287 (MQ=255)
                                                                                                                                                          tGCGGAAAGCTCCGACCGACGAAGTCCGCCGGGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAaca                                                                                                                               <  2:60111/289‑1 (MQ=255)
                                                                                                                                                            cgcAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACTTATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATagc                                                                                                                                                >  1:192201/1‑269 (MQ=255)
                                                                                                                                                                             agaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTg                                                                                                                                                     <  1:44983/248‑1 (MQ=255)
                                                                                                                                                                             agaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTg                                                                                                                                                     >  2:44983/1‑248 (MQ=255)
                                                                                                                                                                                                                aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCaa                                                                                                                                                                                                 >  1:447077/1‑169 (MQ=255)
                                                                                                                                                                                                                aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCaa                                                                                                                                                                                                 <  2:447077/169‑1 (MQ=255)
                                                                                                                                                                                                                    tGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGctc                                                                                                                                                                          >  2:70688/1‑188 (MQ=255)
                                                                                                                                                                                                                    tGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGctc                                                                                                                                                                          <  1:70688/188‑1 (MQ=255)
                                                                                                                                                                                                                       gCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGTCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCCCTAGGTAGGAACTTTGTCGTGGATCGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAACTTGGCTGCTAGAAAGTCGATGATTTTTTGCGTATGATTaaaaa                                                                  >  1:244959/1‑289 (MQ=255)
                                                                                                                                                                                                                        cGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAac                                                                                                                                <  2:343617/226‑1 (MQ=255)
                                                                                                                                                                                                                        cGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAac                                                                                                                                >  1:343617/1‑226 (MQ=255)
                                                                                                                                                                                                                                tGGCCGTATCTGCAATACATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTa                                                         <  1:81810/289‑1 (MQ=255)
                                                                                                                                                                                                                                       aTCGGCCATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCt                                                   <  2:32473/288‑1 (MQ=255)
                                                                                                                                                                                                                                                  aTCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGAt                                       <  1:218343/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACt                                                                                                                                                              >  2:426990/1‑166 (MQ=255)
                                                                                                                                                                                                                                                      tGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACt                                                                                                                                                              <  1:426990/166‑1 (MQ=255)
                                                                                                                                                                                                                                                        cAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGCAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATAAATGGATTTGCTa                                 >  2:454210/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           tgtCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCt                                                   <  1:105637/252‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tgtCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCt                                                   >  2:105637/1‑252 (MQ=255)
                                                                                                                                                                                                                                                                                tGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGaaa         >  2:18489/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     tGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTa    >  1:161486/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                       gCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTaag  >  1:328643/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                       
CCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAG  >  NC_000913/2772782‑2773349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: