Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,850,850 A→G 6.4% N81N (AAT→AAC)  ilvN ← acetolactate synthase 1 small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,850,8500AG 6.4% 206.0 / 4.2 62N81N (AAT→AAC) ilvNacetolactate synthase 1 small subunit
Reads supporting (aligned to +/- strand):  ref base A (32/26);  new base G (2/2);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

ATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGC  >  NC_000913/3850565‑3851122
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                
atCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGCTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGAtt                                                                                                                                                                                                                                                                                >  1:433697/1‑288 (MQ=255)
                  cacaCCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACa                                                                                                                                                                                                                                                             >  1:172364/1‑289 (MQ=255)
                  cacaCCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGATGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCCAGCCTTGAGCGGTTACTGAATAAACACCGCGAACTTGTTAAACATCGTCGGCACGGACTGATTCCGCCGCCATTTCCCGACa                                                                                                                                                                                                                                                             >  2:7899/1‑289 (MQ=255)
                       cgcgCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCtt                                                                                                                                                                                                                                                         >  1:91123/1‑288 (MQ=255)
                       cgcgCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCtt                                                                                                                                                                                                                                                         >  1:227486/1‑288 (MQ=255)
                        gcgcCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGCTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTcc                                                                                                                                                                                                                                                       <  2:433697/289‑1 (MQ=255)
                         cgcCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTcc                                                                                                                                                                                                                                                       >  1:376500/1‑288 (MQ=255)
                              ccgCAGCCCGCCCAGGGCCTCGCGCCCGGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTa                                                                                                                                                                                                                                                 <  2:227486/287‑1 (MQ=255)
                                   gCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGGTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGAt                                                                                                                                                                                                                                            >  1:469858/1‑289 (MQ=255)
                                              gcCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTatca                                                                                                                                                                                                                                 >  1:337/1‑289 (MQ=255)
                                                  cgcgCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTg                                                                                                                                                                                                                             <  1:11035/289‑1 (MQ=255)
                                                  cgcgCCCCGAACCAAACCCGGCAACTCCCTGCAAATCAGGGTCCAGCCCCAGCAGGTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTg                                                                                                                                                                                                                             <  2:376500/289‑1 (MQ=255)
                                                       cccGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGCCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCa                                                                                                                                                                                                                        >  1:225295/1‑289 (MQ=255)
                                                          gAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTTCCCTTTCACGACATTTTCCAGCTTAGCGATTTGGCTTTTCATCTGCTCCAGa                                                                                                                                                                                                                      >  2:11035/1‑288 (MQ=255)
                                                                     ggCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGa                                                                                                                                                                                                                                                                                  <  1:332359/217‑1 (MQ=255)
                                                                     ggCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGa                                                                                                                                                                                                                                                                                  >  2:332359/1‑217 (MQ=255)
                                                                       caccTACCTGCAAATCATGTTCCAGCCCCAGCAGCGGGGCAAAGCCGGAGCGGACGATGAGGTGGTCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCAtt                                                                                                                                                                                                        <  2:172364/286‑1 (MQ=255)
                                                                          cTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGa                                                                                                                                                                                                      >  1:447250/1‑288 (MQ=255)
                                                                               tGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTa                                                                                                                                                                                                >  1:233311/1‑289 (MQ=255)
                                                                                gCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAg                                                                                                                                                                                               <  1:305162/289‑1 (MQ=255)
                                                                                 cAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCTGACGATGAGGTGATCGCCTATAAGGGCAACGGTGATCATGGTCTTGGCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAg                                                                                                                                                                                               <  2:225295/288‑1 (MQ=255)
                                                                                                 cccAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCttt                                                                                                                                                                                 <  2:470287/286‑1 (MQ=255)
                                                                                                                 aGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCg                                                                                                                                                               >  2:188389/1‑288 (MQ=255)
                                                                                                                 aGCCGGAGCGGAAGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCGTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCgg                                                                                                                                                              <  1:478030/289‑1 (MQ=255)
                                                                                                                           gACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGGTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGa                                                                                                                                                    >  1:476031/1‑289 (MQ=255)
                                                                                                                                                     cAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCgcggcg                                                                                                                          >  1:135558/1‑289 (MQ=255)
                                                                                                                                                      aaCGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGcggcgg                                                                                                                         >  1:49872/1‑289 (MQ=255)
                                                                                                                                                            gATAATGGTCTTGGCCTGGTGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCTATGTTGTTCAAGCCGTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCaaa                                                                                                                    <  2:447250/288‑1 (MQ=255)
                                                                                                                                                                         tCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAACCGGCAGACCAAGAATGCCTTCAACGTTCAAAGCGCGGCGGGCAAAAAGGCCACAAATGt                                                                                                      >  2:241770/1‑289 (MQ=255)
                                                                                                                                                                              gCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGt                                                                                                  >  2:23674/1‑288 (MQ=255)
                                                                                                                                                                                   tAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGcc                                                                                                                                                                                             <  2:91556/192‑1 (MQ=255)
                                                                                                                                                                                   tAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGcc                                                                                                                                                                                             >  1:91556/1‑192 (MQ=255)
                                                                                                                                                                                          aaTACGCGCTTACCTGAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGa                                                                                     <  1:147302/289‑1 (MQ=255)
                                                                                                                                                                                                cgcTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCa                                                                                                                                                                                                                                                                        <  1:481117/104‑1 (MQ=255)
                                                                                                                                                                                                cgcTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCa                                                                                                                                                                                                                                                                        >  2:481117/1‑104 (MQ=255)
                                                                                                                                                                                                 gcTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGGTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGc                                                                              <  2:476031/289‑1 (MQ=255)
                                                                                                                                                                                                           aCGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAg                                                                    >  1:260043/1‑289 (MQ=255)
                                                                                                                                                                                                                aaGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGCCAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGGGAGCTCCa                                                               >  1:54143/1‑289 (MQ=255)
                                                                                                                                                                                                                 aGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGAt                                                                                                                                                                                                                                            >  2:349453/1‑115 (MQ=255)
                                                                                                                                                                                                                 aGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGAt                                                                                                                                                                                                                                            <  1:349453/115‑1 (MQ=255)
                                                                                                                                                                                                                 aGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGCTGGTTGCGAACGGTGAGCTCCAg                                                              >  1:138551/1‑289 (MQ=255)
                                                                                                                                                                                                                 aGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTg                                                                      >  1:420590/1‑281 (MQ=255)
                                                                                                                                                                                                                 aGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTg                                                                      <  2:420590/281‑1 (MQ=255)
                                                                                                                                                                                                                 aGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAg                                                              >  1:353291/1‑289 (MQ=255)
                                                                                                                                                                                                                        tgttgtTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACg                                                       <  2:135558/289‑1 (MQ=255)
                                                                                                                                                                                                                             ttCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCaaaaa                                                                                                                  >  1:131673/1‑225 (MQ=255)
                                                                                                                                                                                                                             ttCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCaaaaa                                                                                                                  <  2:131673/225‑1 (MQ=255)
                                                                                                                                                                                                                             ttCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGACCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGttcta                                                  >  2:73747/1‑286 (MQ=255)
                                                                                                                                                                                                                                  gCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCgg                                                                                                                                                              >  1:265267/1‑176 (MQ=255)
                                                                                                                                                                                                                                  gCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCgg                                                                                                                                                              <  2:265267/176‑1 (MQ=255)
                                                                                                                                                                                                                                            gTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTc                                                  >  1:129042/1‑274 (MQ=255)
                                                                                                                                                                                                                                            gTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTc                                                  <  2:129042/274‑1 (MQ=255)
                                                                                                                                                                                                                                             ttACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACg                                                       <  2:42249/268‑1 (MQ=255)
                                                                                                                                                                                                                                             ttACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACg                                                       >  1:42249/1‑268 (MQ=255)
                                                                                                                                                                                                                                              tACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGGTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCa                                 <  2:469858/289‑1 (MQ=255)
                                                                                                                                                                                                                                              tACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCa                                 <  2:260043/289‑1 (MQ=255)
                                                                                                                                                                                                                                              tACTGAAAAAACACCGCGATCTGTTTAAACATCTTCGGATCGGACTGATTACGCTGCACATTCACGACAGCTGCCAGCTTATCGATTTGCGTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCCGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCa                                 <  2:67431/289‑1 (MQ=255)
                                                                                                                                                                                                                                                       aaCACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAACAGCGCGGCGGGCAAAAAGGCCACAAACGTGTGCCATTAAGCCCGGATGGTTGCGAAGGGTGAGATCCCGAATTACGTTGTCATTGGTTGTGTTTTCCATGGCTtttt                        >  2:174252/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           ccGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCg                                                                                                                                                                            <  2:287102/137‑1 (MQ=255)
                                                                                                                                                                                                                                                           ccGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCg                                                                                                                                                                            >  1:287102/1‑137 (MQ=255)
                                                                                                                                                                                                                                                             gcgATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCcca                  <  1:23674/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gcgATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCcca                  <  2:49872/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ttGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCAttt            <  1:188389/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              cgtcgGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTcgc  >  2:357032/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                
ATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGC  >  NC_000913/3850565‑3851122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑