| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,094,415 | T→G | 14.5% | intergenic (‑143/+308) | rhaD ← / ← rhaA | rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,094,415 | 0 | T | G | 14.5% | 78.2 / 9.2 | 32 | intergenic (‑143/+308) | rhaD/rhaA | rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase |
| Reads supporting (aligned to +/- strand): ref base T (16/11); new base G (2/3); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
GCGTCAGGTTGCCGCCGTTGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCT > NC_000913/4094170‑4094702 | gCGTCAGGTTGCCGCCGTTGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCTGGCAATCAATGCCTGATGCg > 1:369215/1‑289 (MQ=255)gCGTCAGGTTGCCGCCGTTGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCCATGCCTGATGCg > 1:186002/1‑289 (MQ=255) cGTCAGGTTGCCGCCGTTGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAgacgcgac < 1:227745/258‑8 (MQ=255) ccgccgTTGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCCCATCCGGCAATCTCAGCCTGATGCCCCGCTGTcgag > 2:89533/1‑287 (MQ=255) tGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATc < 1:469560/289‑1 (MQ=255) tGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATc < 1:324066/289‑1 (MQ=255) aacccAGCCTTTCAGGCAGGCGGCGGTGGGGGGTTTGGTCATTCCCTGGACAAACCCGGACTGAGTAATGTGTTGCGTGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTa < 2:287710/287‑1 (MQ=255) aaGCCTTTCAGCCGGGCGTCGTTGGTGGCTTGGAGCGTTCCCGGGACAAACCAGGACTGGGTAAGGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGGTGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAc < 2:1594/288‑1 (MQ=255) cccTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTTCCg > 1:342683/1‑288 (MQ=255) aGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTg < 2:405273/232‑1 (MQ=255) aGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTg > 1:405273/1‑232 (MQ=255) tAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTTTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTAACACTGTTGCCGACCTGTAGGCCGGATAAGGCCCTTGTGCCGCATCCGGCCATCACTGCCTgtt > 2:111764/1‑286 (MQ=255) gCATGTTCTGTGTCCCTGTAATTTCGGTGTTGTCGTATGAACGAGCCGTAGGCCGGATAAGGCGCTCGCGCCGCAGCCGGCAATCAATTCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACACCTATGGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCTGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGt < 2:369215/289‑1 (MQ=17) caggtgcggtggTCCTGGAAACTCGGTGGTGGCGAATGAACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTGATCAGGCCTACCACTATGGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGt < 2:186002/280‑1 (MQ=14) cATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAATGCCCTTGTTCCGCATCCGGCAATCCATTCCTGATGCGACCCttg > 2:255818/1‑286 (MQ=255) ttCTGGGTTCCTGTAAATTCGTGGTGGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg < 2:135448/286‑1 (MQ=255) gtgcctggcaaTTGGGTGTGGTCGAAGGCACGACCCGTGGGCCGGATAAGGCGCTCGCGCCGCAGCCGGCAATCAATGCCTGATGCGACGCTGTCGCTTCTTATCAGGCCTACAACTATGGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTAt < 2:151581/279‑1 (MQ=14) cACGGCCCGTGGTCCGGATAAGGCGCTCGCGCGGCATCCGGCAATCAATGCCTGATGGGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAgg < 2:342683/289‑1 (MQ=255) tCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGt > 1:352837/1‑132 (MQ=35) tCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCCGGCCTACAACTTTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGCCCCTGTCGCGTCTTATCAGGCCGACAACTATTTCCCACCTTCTGGCCGGATTCGGCCCTTGGGccccctc > 2:194789/1‑283 (MQ=11) tCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGCTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTATGCCGGATAAGGCACTTGTGCCGCATc > 1:120622/1‑288 (MQ=255) cgcgCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTCCAACTCTTGCCGACCTGTAGGCTGGATAAGGCACgtgttgcccatcg > 2:147570/1‑279 (MQ=255) cAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgcg < 2:394804/262‑1 (MQ=255) aCTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCgg < 2:55397/231‑1 (MQ=38) aTTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCACTCAATGCCTGATTCGACGCTGTCGCGTCTTATCAGGCCTACCACTGTTGCCGACCTGTAGGCCGGATAAGGCCCTTGTGCCGCATCCGGCAATCAATGCCTGATTCGACGCTGTCGCGTCTTATCAGGCCTACAACTCTTGCCGACCTGTAGGCCGGACAAGGACCTCGTTCCGCATCCGGCAATCCATTCCTGCTTCGACGCTGCCGCGTCTTCTCACGCCTCCCACTGTTTccaccacct > 2:247045/1‑282 (MQ=14) ttGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGCTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTCTTGCCGACCTGTAGGCCGGACAATGCACTTGTTCCGCATCCGGCCATACACACCTGATGCCAAGCAGTAGCGGCCTACCATGCCTCCCACTGTTTCTGAccctt > 2:231501/1‑286 (MQ=14) cGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAgg > 1:394804/1‑262 (MQ=37) aCCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGc > 1:55397/1‑231 (MQ=255) aCCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAAGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCCATGCCTGATGCCACGCTGTCCCGTCTTACCAGGCCTACAACTGTTCCCCAccctccagccg > 2:362578/1‑280 (MQ=11) cTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGAt > 1:188439/1‑289 (MQ=255) ggCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCCATCAAAGCCCGCTGCGACACCGTCGCGTCCTTGCCGGCCTACAAATGGTTCCGACCCCGAGGCCCGATCAgg > 2:209493/1‑288 (MQ=14) cTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCt < 1:89533/289‑1 (MQ=255) | GCGTCAGGTTGCCGCCGTTGCGCTCATCCCAGCCTTTCAGCCAGGCGTCGGTGGTGGCTTTGATCATTCCCTGGACAAACCAGGACTGAGTAATGTTTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCT > NC_000913/4094170‑4094702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |