| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,560,455:1 | +G | 100% | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 91.7% | 135.9 / 2.0 | 48 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (35/9); total (36/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAC > NC_000913/3560234‑3560675 | cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATG‑ATGG‑cgacgga > 1:687576/1‑222 (MQ=255) tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGGGCCGCACCGCCAAG‑ATGG‑CGCA‑GGacacg > 2:35198/1‑225 (MQ=255) aaTGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGGTTTGCTCCGCCAGc > 2:171498/1‑229 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGTCGCGCCGCACCGCCATT‑GTGT‑CGCA‑GGATCAGGTTTTCCTCCGCCAGCt > 1:169504/1‑228 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCG‑GGACCGGGGTTTGCTCCGCCACCTc > 2:48094/1‑229 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCAACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGCCACCCCCGCACCGCCACGCGGGG‑C‑CA‑GGATCGGGTTTGGATCGGccgaccc > 2:576795/1‑224 (MQ=255) tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTTGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTcgcg > 1:294946/1‑229 (MQ=255) gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GAATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGGCTCCg > 1:308254/1‑229 (MQ=255) cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg > 1:411124/1‑229 (MQ=255) cgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCGGCCCATTCGGGCCCGGGCGAAGAGTcgggtgggtg > 2:296388/1‑220 (MQ=255) aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCTTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc > 1:494273/1‑227 (MQ=255) aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑CTGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg > 1:53325/1‑228 (MQ=255) tCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCCCATTGCGGTCGCGGCGAAAAGTCTGCGGGCTGACGGGGaaa > 1:133069/1‑228 (MQ=255) gCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTTGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGACGAATATTCTGCGGGCTGACGGAGAAATGCTCTACCACCTCTTCGGTACTgcaata > 1:612373/1‑224 (MQ=255) cGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGATTAGCCTGGGGGCTGACGGAGAAATGCCTTACCAACCCTTCGGAACTGACaaaa > 2:666942/1‑229 (MQ=255) gcccATTGGGGGTTTCGCGCCGCCCGTTTGGGGGCGATGCCCTTTTTTTCTGCGGTTGGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct < 1:48094/227‑1 (MQ=255) aGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGGAATGCTCTACCAGCCCTTCGGGACCGCCCTAACCctg > 1:454562/1‑229 (MQ=255) ttGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGAAGGCTGCGGGCTGACGGAGAAATGCTCTCCAAGCCCTTCGTTACTTCCATAACCCTCCTCtt > 2:402232/1‑229 (MQ=255) tt‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCCCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAg > 1:692303/1‑228 (MQ=255) ggccccccctgtttGCGGGCGAGTGTCTTTTTTTCGTCGGTCTGGGTGGCCTTGCGTTCGTGCCACGGCGTGTAAACCGAACTGGAGGGCTGCCCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:151654/218‑1 (MQ=255) ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTTCGGGCTGGCGGGGAAATGCCCTCCCAGCCTTTCGGTATTACCATAACCCTGCTGTTTAACCAGTTCGat > 2:14684/1‑229 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGatg > 1:444610/1‑228 (MQ=255) gccttttcgCGGGTGAGGCACTCTTTTGTGTGGGTCTGGGTGTCTTGGCGATCGTGCCTCGGCGTGTTAACCAATCTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGata < 1:666943/220‑1 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt > 1:646258/1‑229 (MQ=255) ggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGCCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGCCCTAACCCTGCTGTTTAACCCGTTCGTTAATACCGTTGTGa > 1:115188/1‑229 (MQ=255) aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTCTTGACCTAACCCTGCTGTTTAACCCGTTCGTAAATACCGTGTTGCCGtt > 1:349447/1‑229 (MQ=255) cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct < 1:49442/180‑1 (MQ=255) cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct > 2:49442/1‑180 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGAAGCGCCCCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGGCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGAACTGATATAACCCTACTGTTTAACCAGTTCGCTACTACCGTTATTACGTTGTGTTTGTTTCTTTta > 2:5103/1‑229 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCTAATACCGTTTTGACGTTTGGTTTGTTTCCtttt > 1:514192/1‑228 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCACTTCGATAATACCGTTGTGACGTGTGGTTTGTTTCAtttt > 2:632939/1‑228 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCTTTAATACCGTTGTTACGTTGTGTTTGTTTCATTta > 1:531430/1‑229 (MQ=255) ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGGGCCGTTTTGTTTGTTTCATTTAAAAATcc > 2:259794/1‑229 (MQ=255) cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACACAACCCTGCTTGTTAACCAGTTCGAAAATACCGTTGTGACGTGAGTTTTTTTTCTTTTAAAAATCCCTGGaa > 2:149646/1‑229 (MQ=255) gTGCCACGGCGTGTTAACCGAACTGTAAAGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCCCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCCGCTCTTCGGTCCTGACCACACCCTGCTGCTTAACCAGTTCGTTACTACCGTTTGTACGTTTCATTTTTTTCATTTATAAATCCCGGGAATTAttttct > 2:438082/1‑228 (MQ=255) gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCCGTTTAACCAGTTCGATAATACCGTTGTGGCGTTTTGTTGGTTTCTTTTTTAAATCCCTGGAATTAttttcg > 2:742513/1‑229 (MQ=255) gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTTTGACGTTGTGTTTGTTTCATTTATAATTCCCTGGAATTAttttcg > 1:548752/1‑229 (MQ=255) gggggtgtTAACCGAACTGGAAGGCAGGGCCGCCCCGCCATG‑GTGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtttt < 1:287691/223‑1 (MQ=255) gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAACTGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCCGTTCGCTAATACCCGTGGTACGTTTCGTTTGTTTCATTTTTAAATCCCCGGAATTATTTTTGTTTTTGCGCAtt > 2:287691/1‑229 (MQ=255) ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg > 1:58940/1‑229 (MQ=255) aCTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTTCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt < 1:632940/228‑1 (MQ=255) ggcgcccgacccgccgTG‑ATGG‑CGCA‑GGATCCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCTGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAACAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc < 2:294946/219‑1 (MQ=255) ggtgcggctccGCCATG‑ATGG‑CGCA‑GGATCAGGTTTGTCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGGCGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 1:414392/220‑1 (MQ=255) ggcgcCGCCCCGGCAGG‑ATGG‑C‑GAGGGATCAGGTGTGGCTCCGACAGCTGCTTGAGGTAGCGGCGAATAGTCTGGGGGCTGACGGAGAAATGCTCTTCCAGCTCTTCGGTACGGACATAACCCTGCTGTTTAACCAGTTCGATAATCCCGTTGTGACGTTGTGTTTGTTTCAGTTAGAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc < 2:129388/227‑1 (MQ=255) gccccgcACCGCCATG‑ATGGCC‑CA‑GGATTCGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa < 1:44750/226‑1 (MQ=255) cgcccggACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa < 1:171498/226‑1 (MQ=255) cgccgcTCCGCCTTG‑GTGG‑C‑GACGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 2:58940/227‑1 (MQ=255) cgccgcACCGCCATG‑ATGG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTAGTTTTCGCGCATTTAGCGAATCAACAAAAACCaa > 2:351697/1‑228 (MQ=255) tg‑atgG‑CGCA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATGGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCCCGCATTGAGCGAATCAACAAAACACATCGCTAAACCCAc > 1:488369/1‑228 (MQ=255) | CGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTT‑GCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑ATGG‑C‑CA‑GGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAC > NC_000913/3560234‑3560675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |