Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,079,975 A→G 18.9% L206P (CTC→CCC)  yeeA ← putative transporter, FUSC family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,079,9750AG18.9% 147.7 / 2.6 73L206P (CTC→CCC) yeeAputative transporter, FUSC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (29/26);  new base G (12/4);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAGCAGTATTTGCTGCATCATGTGCTGGGTATCACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGA  >  NC_000913/2079750‑2080195
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               
cTCAGCAGTATTTGATGCATCATTGGCTGGGTTTCACGAAGGTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGt                                                                                                                                                                                                                               <  1:281824/228‑1 (MQ=255)
   agcagTATTTGCTGCATCATGTGCTGGGTATCACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGGCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGGGCAATCAGTCCACGCATTTTCCTGGGATCGGTCAGGGGgtttt                                                                                                                                                                                                                           >  1:210633/1‑229 (MQ=255)
            tgctgaATCATGTGCTGGGTATCACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATGCCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATgg                                                                                                                                                                                                                   <  1:89163/228‑1 (MQ=255)
                ctcgcatGTTCTGGGTCTCCCGGAGGTTTTTGCCGTTTCATGACCCGAAATGGCTGGGGCGCGTGGCCCAGTATTCGTTGATTTGCACATCCAGCAGACAAACCAGATTTCGGTTAATTGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTc                                                                                                                                                                                                              <  2:756525/225‑1 (MQ=255)
                      tgtgCTGGGTATCACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCCACCCTTTTTCCGCGCTCCGTCCGGGGTTTTTTTGGATGGGTTTTCacccg                                                                                                                                                                                                        >  2:426028/1‑225 (MQ=255)
                         gCTGAGTACCACGAAGGTTTTCCGCGTTGAATAACTCGAAATGTCTGGCGCGCGGAGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCGTCATATATCGCATTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAAACAATCCGAGCGTTTTCAAGGCATCGGTCAGGGGTTTTTGGAGATGGATTTCCAGACGttg                                                                                                                                                                                                    >  2:172232/1‑227 (MQ=255)
                             ggTATCACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGt                                                                                                                                                                                                <  1:535442/229‑1 (MQ=255)
                             ggTATCACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTCCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCAGACGTGGGCGt                                                                                                                                                                                                >  1:406897/1‑229 (MQ=255)
                                  cccGAATTTTTTGCGGGTTTAATAACACGAAAGGGGCGGGGGGGGGGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTCCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAg                                                                                                                                                                                           <  2:406897/227‑1 (MQ=255)
                                  cACGACGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGGAATCAGTCCACGCCTTTTCACGGCATTGGTCAGGGGTTTTTTTAGATGGCTTTCCCGACGTGGGCGTTCggg                                                                                                                                                                                           >  1:58669/1‑227 (MQ=255)
                                  cACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCATACGTGGGCGTTCggg                                                                                                                                                                                           >  1:61289/1‑227 (MQ=255)
                                     cgtaGTTTTTGCGGGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCAGTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATTCCTTCATATATCGATTTTGGAATGCGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGt                                                                                                                                                                                          <  1:216459/225‑1 (MQ=255)
                                        aGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCCGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGCTTTTGGAATACGGGTTTCTTTGCTGCCGGGCGCAACCACTCCACGCCTTTTCAGGGCATCGTTAAGGAGGTTTTTGAGATGGGTTTCCAGGCGTTGGGGTTCGCGTAAGt                                                                                                                                                                                      >  2:232999/1‑229 (MQ=255)
                                          tttttGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGCCCACGCATTTTTACGGTACCGGTCAGGAGGTTTTTTAGATGGCCTTTCAGACGAGGGCGTTCGAGTAAGtt                                                                                                                                                                                     >  1:68590/1‑228 (MQ=255)
                                          tttttGCGCGTTCAATAACACGAAATGCCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCGGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCCCGCATTTTCACGTCATCGGGCAGGAGTTCTTGTAGATGGCTTTCCAGACTTGGGCGTTCGataaaagtc                                                                                                                                                                                    >  1:230421/1‑221 (MQ=255)
                                              tGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGCTAATGGTCTGGTTACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCCGAATCAGTTCCCACCATTTCACGGCATCGGGTCGGGGTTTTTTTTGATGGCTTTCCCACCTTGGGGGGTCGAGGAAGTTcaggg                                                                                                                                                                                >  2:427513/1‑225 (MQ=255)
                                                    ttCAATAACACGAAATGGCTGTGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGGCTGGATACCTTCATATATCGATTTTGGAATCCGGGTTTCTTTGCTGGCGGGCGCAATTAGTCCCCGCATTTTCCCGGCACCGGGCAGGAGTTTTTGTAGATGGCTTTCCCAATGTGGGCGGTCGAGTAAGttgtgtggggaag                                                                                                                                                                          >  1:334087/1‑218 (MQ=255)
                                                            cacGAAATGGCTGGGGCGCGTGGCCCAGTATGGATTGATTTGCAACTCCAGCATACAAATCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTg                                                                                                                                                                   <  2:562000/228‑1 (MQ=255)
                                                              cGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGGTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCACCGGTAAGGGGGTTTTGTGGGAGGCGTTCCAGACGCGGGCGTTCGGGGAAGTTCGGGGGGAATGCAGATTGATa                                                                                                                                                                >  1:136308/1‑229 (MQ=255)
                                                               gAAATGGCTGGGGTGCGTTGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCGGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATa                                                                                                                                                                <  1:158923/228‑1 (MQ=255)
                                                                 aaTGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGGGTTTTTGTGAGTGGGTTTCCGGGCGTGGTGGTTTGGGTAAGGTCGGTGTGAATGcgagttttatga                                                                                                                                                              >  1:712705/1‑217 (MQ=255)
                                                                 aaTGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCCGACGTGGGCGTTCGGGTAAGTTCGGTGAGAATGCAGATTGATAGa                                                                                                                                                              >  1:22005/1‑228 (MQ=255)
                                                                  aggggtGGGGCGCTGTGCCCAGTATTGCTTGTATTGCAAACCCAGGATACAAACCAGATTGCGGTTAATGGGCTGGGTTCCTTCATATATCGATTTTGGAATGCGGGTTTCTTTGCTGGCTGGCGCAATCAGTCCACGCATTTTCACGGCAGCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAcc                                                                                                                                                            <  2:334087/224‑1 (MQ=255)
                                                                                   cccAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCCATCAGTCCACGCATTTTCACGGCATCGGTCAGGGGGTTTTGTAGATGGCTTTCCCGCCGTGGGCGTTCGGGTAAGTTCGGTGGGAATGCCGATTGAAAGACCCGGTTATAATCGGTCAg                                                                                                                                           >  2:238015/1‑229 (MQ=255)
                                                                                           gCATTGATTTGCAACTTCAGCATACAAACCAGAGTGCGGTTAATGGTCTGGATTCCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATGCTCGGTCAGACTTTTCg                                                                                                                                   <  1:232999/229‑1 (MQ=255)
                                                                                               tGATTTTCAATTTCAGCATGCAAACCAGATGTCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTGCTTTGCCGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAg                                                                                                                               <  1:470828/229‑1 (MQ=255)
                                                                                                 aTTTGGAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCGGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGtt                                                                                                                             <  1:585715/229‑1 (MQ=255)
                                                                                                 aTTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTTATAGACCCGATTTTATTCGGTCCGACTTTTTGCCACtt                                                                                                                             >  1:515196/1‑229 (MQ=255)
                                                                                                 aTTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCAAGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGGTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGGGTGAATGCTGATTTATAGAACCGATTTTACTCGGTCAGACTTTTCGCCAGtt                                                                                                                             >  1:600948/1‑229 (MQ=255)
                                                                                                  tttGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCCTTTTAACGGCATCGGTCAGGAGGTTTTGTAGCTGGCTTTCCAGACGTGGGGGTTCGAGTAAGTTCGGTGAGAATGCAGATTGGTAGACCCGCTTTTACTCGGGCAGAACTTTTGCCAATTg                                                                                                                            >  2:76878/1‑229 (MQ=255)
                                                                                                           ccAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCCATTATACTCGGTCAGACTTTTCGCCCGTGGaaatggcc                                                                                                                    >  1:736746/1‑222 (MQ=255)
                                                                                                                      aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                                                                                                         >  1:449716/1‑228 (MQ=255)
                                                                                                                      aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGGCAGGAGGTTTTGTAGATGGCTTTCCCGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGGTGAATGGGCCAGTgggttaaa                                                                                                         >  1:442714/1‑222 (MQ=255)
                                                                                                                       ccaagttgtgtttAATGGGCTGGATAACTTCCAATTTGGATTTTGGAATTGGGTGTTCTTTGCTGGCGGGTGCAATCCGTCCACCGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACGCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc                                                                                                       <  2:498599/218‑1 (MQ=255)
                                                                                                                          gATTGCGGTTAATGGTCTGGATCCCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc                                                                                                       <  1:537053/226‑1 (MQ=255)
                                                                                                                           aTTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCCTCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGGGGGAATGCAGATTGATAGACCCGATTATACTCGGGCAAGCTTTTCGCACGTTGAAATCGCCCGTGGGTGAAACCCCgg                                                                                                  >  2:57239/1‑228 (MQ=255)
                                                                                                                           aTTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTAACGGCATCGGTCAGGGGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGGGTAAATTCGGTGGGAATGAAGGTTGAAAGACCCGGTTATACTCGGTCAGACTTTTCGCCACGTGAATTCGCCGGGGGAGGaaacgccgt                                                                                                   >  2:219844/1‑222 (MQ=255)
                                                                                                                           aTTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTGTGCTGGCGGGCGCAATCAGTCCACGCATTTTTACGGCCTCGTTCAGGCGTTTTTGTAGATGACTTTCCAGAACGTGGCTGTCGAGTAAGTGCGGTGTGATGGCGTATTGATAAACCCGATTAGACTCGGTCAGACTTTTTCACATGCGCATGAGCCAGTGGATGAACGCCCGt                                                                                                 >  2:534124/1‑229 (MQ=255)
                                                                                                                                     aTGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAc                                                                                                                                                             <  2:286811/161‑1 (MQ=255)
                                                                                                                                     aTGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAc                                                                                                                                                             >  1:286811/1‑161 (MQ=255)
                                                                                                                                      tGGGCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTGTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGa                                                                                       <  1:732728/228‑1 (MQ=255)
                                                                                                                                           tggactccTTCATATATCGTTTTTGTAATTGGGGTTTCTTTGCTGGCGTGCGCAATCATTCCACGCATTTGCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCg                                                                                  <  2:210633/222‑1 (MQ=255)
                                                                                                                                           tGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAc                                                                                                                                                             <  1:507731/155‑1 (MQ=255)
                                                                                                                                                   ttCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGGGGTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGCGTAAATTCGGTGAGAATGCAGATTGATAGACCCGATTATACACGGTCAGCCTTTTTGCCAGTTGAATGCGACAGTGGATGAAAGGCCGCTTGGGGCGAAAACCGGTaacaaaa                                                                         >  2:499670/1‑224 (MQ=255)
                                                                                                                                                   ttCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGCTCGGTGAGAATGCAGATTGCTAGACCCGATTATAACCGGTCAGACTTTTTGCCAGTTGAATGCGCCCGCGGATGAACGCCCGTTGTGGCCAGATACCGGTAaaccac                                                                         >  1:242104/1‑229 (MQ=255)
                                                                                                                                                     cattttttgATTTTGGAATACGGGTTCATTTGCTGGCGGGTACACAGAGTCCGCGCGTTTGCAGGGCATCGGTCAGGGGCGTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGGAAGTTCGTTGAGAAGGCAGAGTGATAGACCCGATGATACGCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCTGTAAacaacag                                                                       <  2:89395/221‑2 (MQ=255)
                                                                                                                                                     cATATTTCGTTTTTGGAATCGGGGTTTCTTGTCTGGCGGGCGCAATCAGTCCACGAATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACAt                                                                       <  2:46052/229‑1 (MQ=255)
                                                                                                                                                     aatatttCGATTTTGGAATACGGGTTTCTTTGGTGGCGGGTGGAATCAGTCCACGCATTTTCACGGCATCGGTCAGGATTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACAt                                                                       <  2:334447/228‑1 (MQ=255)
                                                                                                                                                       tatatCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCCTTTTCACGGCATCGGTCAGGGGGTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTTGGAATGTGGATTGATAAACCCGATTATACTCGGGCCGACTTTTCGCCAGTTGAATGGGCCAGTGGGTGAACGCCCGGTTGGGCCCAGAAACGGTAAAAAACAttc                                                                     >  2:646382/1‑228 (MQ=255)
                                                                                                                                                       tatatCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGTTTGATAGAACCGGTTATTATCGGTCAGCCTTTTCGCCAGTTGAATGCGCCAGGGGATGAAACCCCGTTGTGGCCACATACCGGTAAAAAAAAtt                                                                      >  2:296364/1‑228 (MQ=255)
                                                                                                                                                         tcgcGATTTTGGGAACCGGGTTTCCTTGTGGGCGGGCGCAATCAGTCCACGAATTTGCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTAGAGTAAGTTCGGTGAGAATGTAGATAGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGc                                                                    <  2:727294/225‑1 (MQ=255)
                                                                                                                                                            cGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGGGTTTTTGTAGATGGCTTTCCCGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGGTTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCCGTTGAATGCGCCCATGGATTAAACCCCGTTGTGGCCAGTAACCGGTGAAAAACATTGcccca                                                                >  2:102026/1‑226 (MQ=255)
                                                                                                                                                            cGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGGGAGAATTCAGATTGATAGACCCGATTAAACTCGGTCAGACTTTTCGCCCGTTTAATGCGCCAGGGGGTGAACGCCCCTTGTGGCCAGATACCGGTAaacacacttgcccgt                                                                >  1:217966/1‑218 (MQ=255)
                                                                                                                                                               ttttGGAATACGGGTTTCTTTGCTGGCGGGCGCAATTAGTCCACGCATTTTTACGGCCTCGGTCAGGAGTTTTTTTAGATGGCTTTCCCGACGTGGGCGTTCGGGTAAGTTGGGGGAGAATGCGGGTTTATAAAACACATCATTCTCGGCAAGACTTTTCGCCCGCTGGATGGTCCCACGGATCAAAGCCCGTTGTGGCCAGATACCGGGAAAACACAATGccctctaa                                                             >  2:113642/1‑223 (MQ=255)
                                                                                                                                                                  tGGTATTCGGGTTTCTTTGGTGGCGGGCGCAAACAGTCCACTCATTTTCACGGCATCGGTCAGGGTGTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTGCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAagag                                                          <  2:22005/229‑1 (MQ=255)
                                                                                                                                                                    ggatgcGGGTTTTGTGGCTGGCGGGCTCAATCAGTCCACGCAGTTTGACGGCAGCGGTCAGGAGTTTTTGGAGATGGATTTCCAGACGGGGGCGTTCGAGTAAGGTCGGGTAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGGTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGcc                                                        <  2:429088/224‑1 (MQ=255)
                                                                                                                                                                                        gtgggctCAATCAGTCCACGCATTTTAACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACGCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCtt                                     <  1:672738/226‑1 (MQ=255)
                                                                                                                                                                                             cgcAATCAGGCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCAt                               <  1:57239/229‑1 (MQ=255)
                                                                                                                                                                                                           cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGCCTTTTCGCCAGTTGAATGCGCCAGCGGATGAACGCCCGTTGGGTCCCGGTACCGGTAAACAACATGTCCCGTAAAACGCCGAGGAGCCAATCACCCGTTCACCATAACGCCCTACCaa                  >  2:237004/1‑229 (MQ=255)
                                                                                                                                                                                                           cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCaa                  >  1:452060/1‑229 (MQ=255)
                                                                                                                                                                                                           cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCACCGCTTCGCCATAACGCCGt                       >  1:697655/1‑224 (MQ=255)
                                                                                                                                                                                                             cATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAAtt                >  1:281294/1‑229 (MQ=255)
                                                                                                                                                                                                             cATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCCCCGCTTCGCCAAAAAGCCGGATCAAtt                >  1:364381/1‑229 (MQ=255)
                                                                                                                                                                                                                 ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtg                                                                                               >  2:618824/1‑146 (MQ=255)
                                                                                                                                                                                                                 ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtg                                                                                               <  1:618825/146‑1 (MQ=255)
                                                                                                                                                                                                                 ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCGGCTTCCCCATAACGCCGTATCAATTTCAc            >  1:296316/1‑229 (MQ=255)
                                                                                                                                                                                                                      ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCCTAACGCCGTACCAATTTCACCttc        >  1:574537/1‑226 (MQ=255)
                                                                                                                                                                                                                        cATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCCGATACCGGTAAACAACATTGCCAGTAAAGAGCCCAGGATAAAATCGCCCCTTCCCCATAACGCCGTAACAAATTCACCTGGCgg     >  2:663523/1‑229 (MQ=255)
                                                                                                                                                                                                                         aTCGGTGAGGGGTTTTTGTAGATGGCTTTCCGGACGGGGGCGATCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGGCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCggg    <  2:736745/229‑1 (MQ=255)
                                                                                                                                                                                                                         aTCGGTCAGGGGTTTTTGTAGATGGCTTTCCAGACGGGGGCATTCGGGTAAGTTCGGTGAGAATGCAGATTGATAGGCCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATCCCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCggg    <  2:574536/229‑1 (MQ=255)
                                                                                                                                                                                                                          ggggTCAGGAGTTTTGGTAGATGGCTTTCCAGGAGTTGGCGTTCGAGTAAGTTCGGTGAGGATGCAGATTGATAGCCCCGATTATACTCGGTCAGACTTTGCGCCAGGTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCgggg   <  1:580125/227‑1 (MQ=255)
                                                                                                                                                                                                                           tggTCAGGTGTTTTGGGAGGTGGGTTTCCAGACGTGGGCGTTCGAGGAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTAAACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGa  <  2:281294/228‑1 (MQ=255)
                                                                                                                                                                                                                           cGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTTTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGGGGATCACACCGCCGCTTCGCCCTAACGCCCGATCAAATTACCCTAGCGGGGa  >  2:412879/1‑229 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               
CTCAGCAGTATTTGCTGCATCATGTGCTGGGTATCACGAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGA  >  NC_000913/2079750‑2080195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: