Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,438	G→A	26.6%	intergenic (‑182/‑25)	ttdR ← / → ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,438	0	G	A	26.6%	59.5 / 5.8	39	intergenic (‑182/‑25)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (2/24);  new base A (2/8);  total (7/32)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGT‑TGACAG‑AGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATT  >  NC_000913/3206221‑3206654                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                cTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GCGGGGG‑AAT‑TTTTC‑C‑A‑ACAAACAa‑aaa                                                                                                                                                                                                                     >  2:437884/1‑229 (MQ=255)         cTTTGGCTAAAGGCCAGGTATTTACCATCGTTAATTTTCCGCAGGTGTGATAAGCGCTGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAg                                                                                                                                                                                                            <  2:741473/229‑1 (MQ=255)         cTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAg                                                                                                                                                                                                            <  1:486105/229‑1 (MQ=255)         cTTTGGCCAAAGGCCAGCTATTCAGCCTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCCC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGa                                                                                                                                                                                                             <  1:108343/228‑1 (MQ=255)            tGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTT‑C‑AACCAAAAACAAAAAA‑ATGGAGt‑t                                                                                                                                                                                                         >  1:599803/1‑229 (MQ=255)            tGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCACGTTGATTCAC‑GACCTGTTAATTACATATTAACGGTGGCAAAACATTTAAAACAAC‑ATTTTTA‑GTGGTAGTTT‑TGG‑GC‑GGTG‑GTA‑TTTTT‑T‑ATACAaaaaa‑aaaaaaatgaggta                                                                                                                                                                                                          >  2:181515/1‑220 (MQ=255)                   aGGCCAGTTTTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCCCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCa‑tg                                                                                                                                                                                                  <  2:50449/227‑1 (MQ=255)                         gtttttctggttCGTTAATTCTCCTCAGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑a                                                                                                                                                                                         <  2:21447/218‑1 (MQ=255)                              tCAGCTTTGTTAATTAGCCGTCGTTGTCATAAGTGCAGTGTATTTCGCAAACCATTTCACTTCCCGGCTCTGGCGCAATACTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGGAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AACAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGta                                                                                                                                                                                    <  2:534453/229‑1 (MQ=255)                                aGCATCGTTAATTTACCGCCGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGta                                                                                                                                                                                    <  1:181515/227‑1 (MQ=255)                                 gCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGtaat                                                                                                                                                                                  <  1:411251/228‑1 (MQ=255)                                    tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTT‑T‑TGACCAATAA‑AAAAA‑ATGGCGG‑TGCCA‑CGATTGGCG‑ACAGtaattatg                                                                                                                                                                              >  1:21928/1‑225 (MQ=255)                                      ggtAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCaa                                                                                                                                                                            <  1:416389/227‑1 (MQ=255)                                      gTTAATTAACCGCAGTTGTGATAAGCTCAGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTGGCGCAATATTCAATCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CGGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCaa                                                                                                                                                                            <  1:238480/229‑1 (MQ=255)                                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAACAATATAAAACCAC‑ATTTTTA‑TTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTT‑T‑TTGCACACAC‑AAAAA‑CCAGAAT‑TGCCA‑GGCTTCGCG‑AAAGAACAAAGCaaa                                                                                                                                                                           >  1:215250/1‑228 (MQ=255)                                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGGG‑AAA‑TTTTT‑C‑AGAAAAAATA‑AAAAA‑CTAGAGTGTGTC‑‑TGTTGAGCG‑AGAAGAATAAGAAAc                                                                                                                                                                           >  1:485848/1‑229 (MQ=255)                                          aTTAACCGGAGTTTTGAATAGCGCAGTGTAATTCGCAAAACCTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATGTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                        <  1:495152/229‑1 (MQ=255)                                           ttATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                       <  2:527468/229‑1 (MQ=255)                                                cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑a                                                                                                                                                                                         <  1:664120/206‑1 (MQ=255)                                                  cAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                 <  1:473486/228‑1 (MQ=255)                                                                      tATTTCGCAAAACATTGCCAGTTCCTGCTTTGGCACAATATTCATTCTTATTTTCGCTTTAATTCAC‑GAACTGTTAAATACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGT‑TGACAG‑AGATTGTCGc                                                                                                                                          <  2:215250/229‑1 (MQ=255)                                                                       aTTTCCAAAAACTTTGCCTTTCCCTGCTCTGGTGAAATATTCATTCTTATTTCCGTGTTTATTCAC‑AACCTGTGAATTAAATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑GTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAACCACCAGGCATTGAATAAGT‑TGACAG‑AGATTGTCGca                                                                                                                                         <  2:389790/229‑2 (MQ=255)                                                                                                                cattcTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                        <  1:541815/159‑1 (MQ=255)                                                                                                                               tttGTTTCACGGAATTTTTAATTACATATTAGCGGTGGCAAAAAATAAAAAACCAC‑ATTTTTA‑GGGGTAGTTG‑GGG‑GG‑GGAG‑AAT‑TTTTC‑A‑AAAAAAATAA‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AATGTAAAAAGCAACAGGAGTTGAATAAGG‑GAACAG‑AGATTGTCGTAATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                  <  2:389136/228‑1 (MQ=255)                                                                                                                                ttGATTCAC‑GAACTGTTAATTCTATTTCCACGGTGGCAAAAAAAATAAAACAAC‑ATCTTGA‑TGGGTAGTTG‑TCG‑GC‑GTGA‑AAT‑TTTGC‑A‑AGACAAATAC‑AAAAA‑CTAGAGT‑AGCTA‑TGATGAAGG‑AAAGTAAAAAGCAACGGTAAGGTAATAATT‑TGACAC‑AGATTGTCGTCAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                <  1:427707/229‑1 (MQ=255)                                                                                                                                               gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTT‑C‑AAACAAAAAC‑AAAAA‑CAGTAGT‑TGGCA‑TGATGAGCG‑AAAGGAAAAAACAAAAAGCAGGGAAAAAAA‑TGTCAG‑AGAATGTGGCTAAAATTATCACCATGATTTTTACCCCAAAGCCCGATGACGTGGGGGGTAAACTAAAACACCTCAAGGaag                                                                   >  1:120624/1‑226 (MQ=255)                                                                                                                                                           tgcaggggtgaaaaaaaaaaaaaACCAA‑TTTTTTA‑GGGGTTGGTT‑GGG‑GC‑GG‑G‑AATGTTTCC‑C‑AAAAAAATAC‑AAAAC‑CGGGAGT‑GGCCA‑GGATGAGCG‑AAATTAAAAAACAACAGGCAGAGAAAAAGT‑TGACGG‑AGATTGACGTAACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtc                                                      <  1:108853/218‑1 (MQ=255)                                                                                                                                                                  tGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGGAGTTT‑GGG‑GC‑GGTA‑AAT‑TTTCC‑A‑AGAAAAATAC‑AAAAA‑CGGGTGT‑TGCCA‑TGATAAGCG‑AAAGTAATAAGAAACGGGCAGTGAATAAGT‑TGACAG‑AGATTGCCGCTACCTTTACCGCCATGATTTCTACCCGAAt                                                                                                            <  1:553293/168‑1 (MQ=255)                                                                                                                                                                   ggaaaaaaaTATAAAACCAC‑ATTTTAA‑GTGGTAGTTT‑GGG‑GC‑GGTG‑AAT‑TTTTC‑C‑AAAAAAATAC‑AAAAA‑CTGGATT‑TGCCA‑TGATAGGCG‑AAATTAATAAGCAACAGGCAGTAAATAAGT‑TGAAAG‑AGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  1:115981/226‑1 (MQ=255)                                                                                                                                                                   ggaaaaaaaTAAAGAACTCG‑ATTGTTA‑GT‑GTGGTTTGGTG‑GT‑GGT‑‑AAT‑TTTTC‑CAAGAAAAAT‑C‑AAAAA‑ATGGGAG‑TGCCAGTAATGATCA‑AAAGTAAAAAGAAACAGGATGTTAATAAGT‑TGAAGG‑ATATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  1:756254/226‑1 (MQ=255)                                                                                                                                                                   gcaaaaaaaTATAAACCCTA‑ATTTTTA‑GTGGTAGTTT‑G‑G‑GG‑GGTGAAAT‑TTTTC‑A‑AGACAAATAA‑AAAAA‑CTGGAGT‑TGCAA‑TGATGCGCA‑AAATTAAAAAACAACGGCCAGTTAATAAGT‑AGACAG‑AGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  1:2100/226‑1 (MQ=255)                                                                                                                                                                     gaaaaaaaaTAAAAACACAATTTTTAGGGGGTAGTTT‑GGG‑GG‑GGAA‑AAT‑TTTAC‑C‑AAAAAAAAAA‑AAAAA‑CGGGGGT‑TGCAT‑AGGGTAGCA‑AAATAAATACACAAGAGGTAATTAATAAGT‑TAAAAG‑AGGTTCGCGTAAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  2:21928/228‑1 (MQ=255)                                                                                                                                                                        aaaaaaTAAAACAAA‑TTTTTTT‑GTGGTAGTTG‑GTGCGC‑GGT‑‑AAT‑TTGGC‑C‑AGA‑AAAAAC‑AAAAAGTTGAAGT‑GACAA‑TGAGGAGCA‑CAATAAACAAGAACGGGGCATTGAATAAGT‑AGAAGG‑ATTTTGGCGCAAAATCTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAACCGTCGTCGATGGGGAAAAtt                                         <  2:660610/229‑1 (MQ=255)                                                                                                                                                                          ttttaaaaaCCAT‑TTTTTTT‑GGGGGTGTTT‑GGG‑GG‑GGT‑‑AAT‑TTTTCAC‑AACAAAAAAA‑AAAAA‑GTGGGGT‑TGCAA‑TGATGAGCG‑AAAGAAAAAACCAACAGAC‑GGGAAAAAGTGTGACAG‑AGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                       <  2:292781/224‑1 (MQ=255)                                                                                                                                                                          atttaaaaaCCAT‑TTTTTAG‑GTGGTAGTTT‑GGG‑GG‑GGT‑‑AAT‑TTTTC‑CAAAAAAAATAA‑AAAAA‑CGGAAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGAAACAGGAGTTGAATAAGT‑TGAAAG‑AGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                       <  2:120624/224‑1 (MQ=255)                                                                                                                                                                                                 tggtggTTT‑GGG‑GC‑GATA‑AAT‑TTTTC‑C‑AGGAAAATAA‑AAAAC‑GGGGATT‑TGCCA‑TGATGGGAG‑AAAGTAATAAGCAACAGGTAGAGAATAAGT‑TGAAGG‑GTCTTGTATATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                  <  1:76812/229‑1 (MQ=255)                                                                                                                                                                                                 tGGTATTTT‑GGG‑GG‑GGTA‑AAT‑TTGTC‑A‑AAAAAAAAAA‑AAAAC‑GTGGATG‑TGCAA‑GGATG‑GCGAAAAGAAATAACCAACGGGAGGGAAATAAGT‑GAAAGG‑TGATGGCCGCTAATATAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                  <  2:280835/229‑1 (MQ=255)                                                                                                                                                                                                                    tG‑ATT‑TTTCC‑C‑AAAAAAATAA‑AAAAA‑CGGGATT‑TGCCA‑TGATGAGAA‑AAAGTAATAAGCAACGGGCAGTGAATAAGT‑TGAAAG‑AGTTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt  <  1:362402/229‑1 (MQ=255)                                                                                                                                                                                                                     g‑AAT‑TTTAC‑C‑AGACAAATAC‑AAACG‑GTGGATT‑TGCCA‑GGTGTGGCG‑AAGTTAAAAGGCAACAGGAAGTGAATAAGT‑T‑AGAGAAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt  <  1:484934/228‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                CTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTG‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGT‑TGACAG‑AGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATT  >  NC_000913/3206221‑3206654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑