| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,741,500 | T→C | 13.1% | E28G (GAG→GGG) | ysaA ← | putative hydrogenase, 4Fe‑4S ferredoxin‑type component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,741,500 | 0 | T | C | 13.1% | 150.5 / 2.3 | 68 | E28G (GAG→GGG) | ysaA | putative hydrogenase, 4Fe‑4S ferredoxin‑type component |
| Reads supporting (aligned to +/- strand): ref base T (29/27); new base C (3/6); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCACCAAACGGGCAAGCCAGCATACAGCTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGTCCAAAGCGTTATGTGATCGCGCCCAGGTCATCCCTGACA > NC_000913/3741273‑3741714 | tCGCACCAAACGGGCAAGCCAGCATACAGCTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATtctc > 1:590177/1‑229 (MQ=255) cGCACCAAACGGGCAAGCCAGCATTCAGCTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATAGGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCa < 1:348020/229‑1 (MQ=255) cGCACCAAACGGGCAAGCCAGCATACAGCTTTTACAGCCCATGCAACGTGTTTGTTCAACCAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCa < 1:242312/229‑1 (MQ=255) gCAAGCCAGCATAAAGCTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGGCTTTAATGACACGAATACGGGAAATAAAATCGTCTGGTGGCAACGCAGCGCAATCCTGATTCACAGAATGCGaaca > 1:609373/1‑225 (MQ=255) aGCCAGCATACAGCTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGGCTTTCATGACACGAATACGGGAAATAAACCCGCCTGGGGACACACCAGCGCAATCCTCATCCCCATAATGGCACA‑CT‑gcc > 2:169592/1‑228 (MQ=255) gcagactgacgCTTTTTCAGCCAATGCCACGTGTTTGTTCAACGAAAATTGGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGCCATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCa < 1:705668/219‑1 (MQ=255) aGCTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGACTTTAATGACACGAATACGGGAAATAAACTCGCCTGGTGACAACCAAGCGCAAACCTGATTCCCATGATGCGACA‑CC‑GCGCAAGCCCCtca > 2:692014/1‑227 (MQ=255) cTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGGGGTCTTTAATGACACGAATACGGGCAATAAACTCGTCTGGTGACAACGCAACGCAACACCGATTCTCCTGAATAGACA‑CT‑GCGCAAGCCACTTaac > 2:171491/1‑229 (MQ=255) aGCCAATGAAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCCCGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACg < 1:388298/229‑1 (MQ=255) cAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGCCACGCATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGAAATCCTGATTCTCATGATGCTCAA‑CT‑GGGCAAGCAACTTCACAGGTCCGCCa > 1:297117/1‑229 (MQ=255) tGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGTGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGGGACACCGCAGCGCAATCCTGATTCTCATGATACGACA‑CT‑ACGCAAGCCACTTCCAAGGACCGGCAAc > 1:739808/1‑228 (MQ=255) aaCGTGTTCTGTCAACTAAAATACGCCCATGTCCCCGGTTTAACGCGTAAACAGGGTAAACCATAGCCCATGGTGCATTTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATGCGGTAAATAAACTCTTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCTCAAGCCACTTCACAGGTACGGCAACCGAt < 1:732439/229‑1 (MQ=255) gtgtTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GGGCAAGCCACTTCACAGGTACGGCACCCGATA‑c > 1:446140/1‑228 (MQ=255) tgttCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACCCTGATGACAGGCTACGGCCGAGGTCCAGCAGTGGTCTTTAATGACCCGAATCCGGGAAATCAACTCGTCAGTTGACACCGCAGCCAAATCCTTATTCGACAGATGCGACC‑CT‑GCGCCAGCCCATTCACAGGAACAGCAAACGAGA‑AATTtcg > 2:695101/1‑229 (MQ=255) aCGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTTATTCTCATGATGCGACA‑CT‑CGGCAAGCCCCTTCCCAAGAAACGCAACCAGAA‑CATTtcgtccccc > 2:724095/1‑225 (MQ=255) aCGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGCAC‑CT‑GCGCAACCCACTTCCCAGGTAGGGCAACCAATC‑AATTTCGCCGCac > 2:687264/1‑228 (MQ=255) cGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCCCATGATGCGAAC‑CT‑GCCCAAGCAACTTCCCAGGGACGCAAACCGAAA‑CATTtcatcgacac > 2:494179/1‑225 (MQ=255) aaaaTATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCCCGGTGCATCTTCAAACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCg < 1:363972/229‑1 (MQ=255) aTGTTCGTGGCTTAACGGGTACACAGGGACGGAATTTGCGCACGGTGCAGCTTACCACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAAc < 1:153608/229‑1 (MQ=255) aTGTTCGCGGCTTATCGGGTCAACAGGGCAGACATTCGCGCCCGGTCCATTTTCACACTGATGACAGGCTACTGCCGGGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAAc < 1:171491/229‑1 (MQ=255) aTGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGTTGCGaca > 2:436444/1‑167 (MQ=255) aTGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGaca < 1:436444/167‑1 (MQ=255) tGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCCCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCACTAATAAAc > 1:48619/1‑228 (MQ=255) cgccgcTTAGCGCGTCAACAGGGCAGACTTTCGCGCACGGTGCATATCCACACGTATGACAGGCAACTGCCTGGGTCCAGCAGTGGTCTTTAATGACACGAATACGGTAAATAAACGCGTCTGGTGACAACGCAGCGCAATCCTTATTCTCAGGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGTTA‑CATTTCGTCGCATCCGCAATAATAAACCGGtt < 1:111852/229‑1 (MQ=255) gcgGCTTATCGCCTCAACAGGGCAGCAATTCGCGCACGGTGCATCTTCACACTGATGACGGGCGACGGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACC‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTc < 2:446140/229‑1 (MQ=255) gcagtctATCGCGTCATACGGGCATCCATTGCCGCACGGTGCATCTTCACTCTAGTGACAGGCTCCTTCCTTGGTCCATCAGTGTTCTTTAATGACACGAATACGGGAAATAACCTCTTCTGGTAACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTc < 1:722567/223‑1 (MQ=255) aTCGCGTCAACAGGTCAGACATTCGCGCCCGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGCAATAAACTCGTCTGGGGACAACGCACCGCAATCCTGATTCTCATGATGCTCCA‑CT‑GCGGAGGCCCCTTACCAGCAACGGCAACC‑ATACCATTACTTACCATCCGCAATAAAAAACCGTGTCATAAtac > 2:443573/1‑227 (MQ=255) cAACAGGGCAGACATTCGCGCCAGGTGCATCTTAACACTGATGACAGGCTAATGCGGTGGTCCAGCAGTGGTCTTTACTGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCtt < 1:615023/228‑1 (MQ=255) agggcgagcagtCGCGCACGGGGCATCTGCACATGTATGACAGGCTACTGCCGTGGTCAAGTAGTGGTCTTCAATGACACGAACACGGGAAATAAACTCTTCTGGTGAAAACCCCGCCCAATCCTGGTTCCCATGATGCGACA‑CT‑GCGCAAGCCCCTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCAc < 2:69936/218‑1 (MQ=255) aGGGCAGCCATTTGCGCAAGGTGCATCTTCCCGCGAAAGACGGGCTACGGCCGTGGTCCAGAAGTGGTCTTAAATGCCAACAATACGGGAAATAAACTCGTCTGGTGACACCGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCAc < 1:351550/229‑1 (MQ=255) aGGGCAGACATTCGGCCAGGGTGAATCTTCACACGGATGACAGGCTACGGCCGTGGCCCAGCAGGGGTTTGTAATGACCCGAATCCGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCAc < 2:590176/229‑1 (MQ=255) cgcgCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCaga > 1:172783/1‑229 (MQ=255) cgcgCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGAGACA‑CT‑GCGCAAGCCACTTCACAGGAACGGCAACCGCTC‑AATTTCGTCGCATCCGCAAAAATAAACCGGTTTTTACTCCCTCCTTTTCCCTAAAacaacaaa > 2:594097/1‑227 (MQ=255) gcgcgcGGTGCATCTTCACACTGATGGCAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAAGGCCACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAAcagca < 1:687265/226‑1 (MQ=255) gcgcACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCCCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAAcagca < 1:386403/226‑1 (MQ=255) ccgccaggTGCTTTTGCACACGGAAGACAGGCTAATGCCGTGGTCCGGCAGGGGTCTTTAATGACAAGAATCCGGGAAATAAACTCGTCGGGTGACAACGCAGCGAAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCaga < 2:428728/222‑1 (MQ=255) gTGCATCTTCACACTGAGGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCaga < 1:518466/229‑1 (MQ=255) tGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCat > 1:395250/1‑226 (MQ=255) cATCTTAACCCGGATGAAAAGGTACTGCCGTGTCCCAGCAGTGGTATTTAATTACACGATGACGGGAAATAAACTCGTCTGGTGACAACCCAGCGCAATCTTGATTACCATTATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCagagag < 2:33490/229‑1 (MQ=255) cATATTCACACTGATGACAGGCTAACGCCGTGGTCCAGCAGTGGTCTTTAATGCCACGAATTCGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACC‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTTATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCagagag < 1:594098/229‑1 (MQ=255) ttCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATCATGCGACA‑CT‑GCGCAAGCCACTTCCCACGGACGGCAACCGATA‑CCTTTCGTCGCATCCGCAATACTAAACCGGTTCCTGATCCCCCCTTTGCACTCAAACCCCAAAGTTCCaagaaacgct > 2:680430/1‑220 (MQ=255) tCACTCTGATGACCGGCTATGGCCTTGGTCCAGCATGGGTCTTTAATGACACGAATACGGGAAATAACCTCGTCTGGTGACAACGCAGCGCAATCCGGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGt < 1:169592/229‑1 (MQ=255) cacTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCCATAATAAACCGGTTCATGATCACTCCTATGCACTAAAACAGCAGACGGCCacagaaccgctcca > 2:728967/1‑220 (MQ=255) cacTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTccc > 2:172791/1‑229 (MQ=255) cacTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTcc > 1:194954/1‑228 (MQ=255) atgaCAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACGGCAGAGTGCCAGAGAACGCGTCCCTAATg > 1:311610/1‑229 (MQ=255) atgaCAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAAAGTGCCAGAGAGCGCGTCCCTACTg > 1:364068/1‑229 (MQ=255) ggCTACTGCCGGGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGa < 1:443573/229‑1 (MQ=255) tGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAAcagca > 2:499373/1‑186 (MQ=255) tGGTACAGCAGTGGTTTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAAcagca < 1:499373/186‑1 (MQ=255) ggTCCCGCAGGTGTTTTTAAAGAATCGAATACGGGAAATAAACCCCGCTGGTCACAACCGAGCGCAATCCTGATTCACATGATGCGACA‑CG‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTATGCACTAAAACAGGAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAgg < 1:728968/228‑1 (MQ=255) tCCAGCAGTGGTCTTTAATGACACGAATCCGGAAAATAACCCCTGTTGGGGACACCGCACCGAAATCAGAATTCCCATGA‑GCGACACCT‑CCGCAAGCCCCTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGaa < 2:194954/228‑1 (MQ=255) cagTAGTCTTTAATGACACGAATACGGGAAATAACCTCGTCTGGTGACAACGGAGCCCAATCCTGAGTCCCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGtt < 1:347044/228‑1 (MQ=255) gccTTTAATGACACGAGTACGGGAAATAAACTCGTCTGGTGACAACGCAGAGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCCACCGATA‑CATTTCGTCGCATCCCCAATAATAAACCGGTTCATGATCACTCCTTTGAACTAAACCAGCAACGTGCCACAGAGCGCGTCCCTACTGCTTTAGTCCACCGATAGAAATGTTAAAGtt > 1:754343/3‑229 (MQ=255) gTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGt > 2:754509/1‑228 (MQ=255) gTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACGGCTTGGATCAAACAAAGGAAATGTTAAAGt > 2:672510/1‑228 (MQ=255) gTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCAGGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTCCTTTGTTCAAACGAAGGAAATGTTa > 1:730822/1‑224 (MQ=255) acacGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTg > 1:345494/1‑229 (MQ=255) gAAGACGGGAAATAACCTCGCCTGGTGACAACGCAGCCCAATCCTGATTCTCATGATGCGACA‑CT‑GCCCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGTCCa < 1:724096/229‑1 (MQ=255) cGGAAAAAAAACTCTGTTGGTAACAACCCCGCGCATGCCTGATTCTAATGAGGCCACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGTCCAAAGCg < 2:364068/229‑1 (MQ=255) gAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAAGAAATGTTAAAGTTTTGGCACTTGTCCAAAGCGtc > 1:727307/1‑227 (MQ=255) aaCTCGTCTGGTTACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGTCCAAAGCGTTATGTGAt < 1:172791/229‑1 (MQ=255) aCTCTTCGGGGGACAACGCAGCGCAATCCTGATTCCCATGATGCGAAA‑CG‑GCGAAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGTCCAAAGCGTTATGTGATc < 1:360856/229‑1 (MQ=255) tCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAAcagca > 2:502306/1‑139 (MQ=255) tCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAAcagca < 1:502306/139‑1 (MQ=255) gtcaCAACGCACCGCAACCCTGATTCTCATTAAGCGA‑A‑CTGGCCCAAGCCACTTCACAGGTTCGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTTCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGTCCAAAGCGTTATGTGATCGCGCCCAgg < 1:692015/226‑1 (MQ=255) gcgcAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACAGCTTTGTTCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGACCAAAGGGTTATGTGATCGCGCCCAGGACATCCCTTAc > 1:688644/1‑229 (MQ=255) gcgcAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCCCGTCCCTACTGCTTTGATCCAACGAAGGAAAGGTTAAAGTTTTGGCCCTTTTCCAAAGCGTTATGTAATCGCGACCAGGCAATcccgcac > 2:338806/1‑225 (MQ=255) cgcAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAACGGTTAAAGTTTTGGCACTTGTCCAAAGCGTTATGTAATCGCGCCCAGGGCATCCCTGACa > 1:608738/1‑229 (MQ=255) | TCGCACCAAACGGGCAAGCCAGCATACAGCTTTTACAGCCAATGCAACGTGTTTGTTCAACGAAAATATGCCCATGTTCGCGGCTTATCGCGTCAACAGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACA‑CT‑GCGCAAGCCACTTCACAGGTACGGCAACCGATA‑CATTTCGTCGCATCCGCAATAATAAACCGGTTCATGATCACTCCTTTGCACTAAAACAGCAGAGTGCCAGAGAGCGCGTCCCTACTGCTTTGATCCAACGAAGGAAATGTTAAAGTTTTGGCACTTGTCCAAAGCGTTATGTGATCGCGCCCAGGTCATCCCTGACA > NC_000913/3741273‑3741714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |