Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,767,039 A→T 6.1% N235Y (AAT→TAT)  yibA → putative immunity protein for polymorphic toxin RhsA; HEAT‑domain protein; lethality reduction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,767,0390AT 6.1% 234.5 / 4.9 66N235Y (AAT→TAT) yibAputative immunity protein for polymorphic toxin RhsA; HEAT‑domain protein; lethality reduction protein
Reads supporting (aligned to +/- strand):  ref base A (37/25);  new base T (2/2);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

GATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGCCGCATTTGCAATAAATATCAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGG  >  NC_000913/3766828‑3767266
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   
taTTAATTTTTTAAAAAATCCAAATGGGGATGTCAGAAACTGGGCCGGATTTGCAATAAATATCAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATgatgat                                                                                                                                                                                                                    <  2:336306/228‑1 (MQ=255)
gATTAAGCTGTTAAAAGATCCAAAGGGAGATGTCAGAAACTGGGCCGCATTTGCAATAAATATCAATAAATATGATAATAGGGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATgatgat                                                                                                                                                                                                                    <  1:416869/229‑1 (MQ=255)
               aGATCCAAATGGAGATGTCAGAAACTGGGCCGCATTTGCAATAAATATCAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCg                                                                                                                                                                                                     <  1:207038/229‑1 (MQ=255)
                    cAAATGGAGATGTCAGAAACTGGGCCGCATTTGCAATAAATATCAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAATGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAACAGAGGGTGa                                                                                                                                                                                                >  2:61470/1‑229 (MQ=255)
                                               cATTTGCAATAAATATCAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATgaggat                                                                                                                                                                                                                    >  1:601939/1‑182 (MQ=255)
                                               cATTTGCAATAAATATCAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGt                                                                                                                                                                     >  1:517868/1‑229 (MQ=255)
                                               cATTTGCAATAAATATCAATAAATATGAAAATAGTGATATTAGGGATTGTTTTGTTGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATgaggat                                                                                                                                                                                                                    <  2:601938/182‑1 (MQ=255)
                                                  ttGCAATAAATATAAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGtttt                                                                                                                                                                  <  1:92161/229‑1 (MQ=255)
                                                  ttGCAAGAAATAGCAATAAATATGATAAATGTGAAATTAGGGATTGATTTGGGGGGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATGGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGtttt                                                                                                                                                                  <  2:263079/229‑1 (MQ=255)
                                                        taaattttaataaatatGATAATAGTGATATTTGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGGCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATa                                                                                                                                                             <  2:744142/228‑1 (MQ=255)
                                                            tatcaataaatatGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTAtg                                                                                                                                                        <  1:61470/229‑1 (MQ=255)
                                                                  taaatatGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGGGATAAAACGCCACTTCTGGTTTTAAATACATTTTTGTAc                                                                                                                                                  >  2:742324/1‑229 (MQ=255)
                                                                      tatGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGGAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGGGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATATAATGTTGTAAAAGt                                                                                                                                              >  2:319035/1‑229 (MQ=255)
                                                                                gTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCt                                                                                                                                                                       <  1:341630/194‑1 (MQ=255)
                                                                                gTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAAAGCTACTTCCt                                                                                                                                                                       >  2:341630/1‑194 (MQ=255)
                                                                                gTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGATTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGGGAATTAGGGGATAAAACGCGACTTCCTGTTTTAGATACTAGGTTGTACAAATTTGATGACaa                                                                                                                                    >  2:434814/1‑229 (MQ=255)
                                                                                   atatTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACGAAAAAGATAAAAGGGTTTTATTTGTTTTATGCGATGAGTTAAAAAAAAATa                                                                                                                                                                                                                                  >  2:112612/1‑132 (MQ=255)
                                                                                   atatTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATa                                                                                                                                                                                                                                  <  2:463349/132‑1 (MQ=255)
                                                                                   atatTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATa                                                                                                                                                                                                                                  <  1:112612/132‑1 (MQ=255)
                                                                                   atatTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATa                                                                                                                                                                                                                                  >  1:463349/1‑132 (MQ=255)
                                                                                   atatTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGTTGAATAAGAGGATAAAACGCTAATTCCTGTTTTAGAAACGATGTTTTAAAAGTTGCATAACAATGa                                                                                                                                 >  2:187182/1‑229 (MQ=255)
                                                                                   atatTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAAGGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGa                                                                                                                                 >  1:83517/1‑229 (MQ=255)
                                                                                   atatTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAAAGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTAAAGACaaaaa                                                                                                                                 >  2:669072/1‑226 (MQ=255)
                                                                                        aGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTCCAAGTTTGATGACATTGAAAt                                                                                                                              >  1:736502/1‑227 (MQ=255)
                                                                                         gggATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAATATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGtttt                                                                                                                                                                  >  1:580760/1‑190 (MQ=255)
                                                                                         gggATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCt                                                                                                                                                                       >  1:290877/1‑185 (MQ=255)
                                                                                         gggATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCt                                                                                                                                                                       <  2:290877/185‑1 (MQ=255)
                                                                                         gggATTGTTTTGTGGAAATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAATATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGtttt                                                                                                                                                                  <  2:580759/190‑1 (MQ=255)
                                                                                             ttGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACaa                                                                                                                                    >  1:657552/1‑216 (MQ=255)
                                                                                             ttGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACaa                                                                                                                                    <  2:657551/216‑1 (MQ=255)
                                                                                                gtttGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGGGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTAATGACAATGAAATTATAACTTcc                                                                                                                    >  1:314587/2‑229 (MQ=255)
                                                                                                     tgGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGGGATAAAACGTTACTTCCTGTTTTAATTAATATGTTGTACAAGTTTGATGACAATGAAATTTAAACTTTCGTTAt                                                                                                               >  2:747113/1‑229 (MQ=255)
                                                                                                          aTGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAATATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTAAGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGaaa                                                                                                          >  2:24321/1‑227 (MQ=255)
                                                                                                          aTGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAGGTTTGATGACAATGAAATTATAACTTCCGTTATTGATa                                                                                                          >  1:512755/1‑229 (MQ=255)
                                                                                                          aTGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATa                                                                                                          >  1:133783/1‑229 (MQ=255)
                                                                                                          aTGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTAAAACTTCCGCTATTGATa                                                                                                          >  2:228887/1‑229 (MQ=255)
                                                                                                               tCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATa                                                                                                                                                                                                                                  >  2:252553/1‑104 (MQ=255)
                                                                                                               tCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATa                                                                                                                                                                                                                                  <  1:252553/104‑1 (MQ=255)
                                                                                                                   gATAAAAATGAGGAAGTCCGTATTGAAGCAATAAGCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAg                                                                                                  <  1:669073/228‑1 (MQ=255)
                                                                                                                        aaaTGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGAt                                                                                                           <  2:536423/214‑1 (MQ=255)
                                                                                                                        aaaTGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGAt                                                                                                           >  1:536423/1‑214 (MQ=255)
                                                                                                                        aaaTGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGAAAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGATTTCa                                                                                            >  2:551328/1‑229 (MQ=255)
                                                                                                                             aGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATAc                                                                                                                                                                                                                                 <  2:528604/91‑1 (MQ=255)
                                                                                                                             aGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATAc                                                                                                                                                                                                                                 >  1:528604/1‑91 (MQ=255)
                                                                                                                                  gTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAATATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGt                                                                                  <  1:24321/229‑1 (MQ=255)
                                                                                                                                   tCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATCCTATGTTGTACAAGTTTGATTACAATGAAATTATAACTTCCCCTATTGATAAGCTGAAGCGTCAAAAGCGTGAGtt                                                                                 >  2:5273/1‑229 (MQ=255)
                                                                                                                                       tATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAATTTCCGCTATTGAAAAGCTGAAGCGTTCAAAGCGGGAGTGGcca                                                                             >  2:358982/1‑228 (MQ=255)
                                                                                                                                         ttGAAGCAATAATCGGACTTTCCTAAAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTa                                                                            <  1:319035/228‑1 (MQ=255)
                                                                                                                                            aaacAATAAGGGGACTTTCCTAAAGAAAAGATAAAAGGGTTTTATCTGTGTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGca                                                                        <  2:736501/226‑1 (MQ=255)
                                                                                                                                                 ataatCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATa                                                                                                                                                             >  2:651134/1‑139 (MQ=255)
                                                                                                                                                 ataatCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATa                                                                                                                                                             <  1:651135/139‑1 (MQ=255)
                                                                                                                                                             tcatACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTATAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAg                                                                                                  <  1:311407/183‑1 (MQ=255)
                                                                                                                                                             tacTAAAGAAAAGATAAAAGGTTTTTATCTGTTTTATGCGATGATTTAAAAAAAAATACATTTTATGATGATATCATTGAAGCGTCGGGTGAATTAGGTGATAAAACGATACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCccgc                                                       <  1:358982/227‑1 (MQ=255)
                                                                                                                                                             tCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACATTGAAATTATAACTTCCGCTATTGATAAGCTGAAg                                                                                                  >  2:311407/1‑186 (MQ=255)
                                                                                                                                                             tCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAACCGTTCTTAGCGTGAGTTGCCTATGCACAGTGGGGGATTCccg                                                        >  1:719160/1‑228 (MQ=255)
                                                                                                                                                                            aaaaGGTTTTTATTTTTTTTATGGGATGAGTAAAAAAAAAATACTGTTTATGATGAAATATTTGAAGCGGGGGGTGAATTAGGGGATAAAACGTTATTTCCTGTTTTAGATACTATGTTGTAAAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGAt                                        <  2:83517/229‑1 (MQ=255)
                                                                                                                                                                                    tttATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGAt                                                                                                           >  2:490890/1‑154 (MQ=255)
                                                                                                                                                                                    tttATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGAt                                                                                                           <  1:490890/154‑1 (MQ=255)
                                                                                                                                                                                            ttttATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGaa                        >  1:503676/1‑229 (MQ=255)
                                                                                                                                                                                                      tgcgTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGTCTGGAAAGTGAAATcc              >  1:181596/4‑229 (MQ=255)
                                                                                                                                                                                                      tGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGCTAAGCTAAAGCGTTCATACCGGGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGAACGGTGCAGATGCGCGACCGGCTGGAAAAGGAAAAcc              >  2:729138/1‑229 (MQ=255)
                                                                                                                                                                                                      tGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGGGTTCATAGCGTGAGt                                                                                  >  2:747504/1‑161 (MQ=255)
                                                                                                                                                                                                      tGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGt                                                                                  <  1:747505/161‑1 (MQ=255)
                                                                                                                                                                                                      tGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCCAAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCGGGAAAGTGAAATcc              >  1:275608/1‑229 (MQ=255)
                                                                                                                                                                                                      tGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGCCCGGCTGGAAAGTGAAATcc              >  1:111219/1‑229 (MQ=255)
                                                                                                                                                                                                      tGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCt             >  1:638528/1‑229 (MQ=255)
                                                                                                                                                                                                            aaaaaaaaaTACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTg                                                                   >  2:424685/1‑170 (MQ=255)
                                                                                                                                                                                                            aaaaaaaaaTACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTg                                                                   <  1:424685/170‑1 (MQ=255)
                                                                                                                                                                                                                  aaaTACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCCCGGGGCAGATGCCCGACCGGCTGGAAAGTGAAATCCGGGAGGGCCggg  >  2:501746/1‑229 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   
GATTAATCTGTTAAAAGATCCAAATGGAGATGTCAGAAACTGGGCCGCATTTGCAATAAATATCAATAAATATGATAATAGTGATATTAGGGATTGTTTTGTGGAGATGCTTCAGGATAAAAATGAGGAAGTCCGTATTGAAGCAATAATCGGACTTTCCTACAGAAAAGATAAAAGGGTTTTATCTGTTTTATGCGATGAGTTAAAAAAAAATACTGTTTATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGG  >  NC_000913/3766828‑3767266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: