Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,790,969 A→C 10.0% V126G (GTG→GGG)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,9690AC10.0% 193.7 / 2.3 79V126G (GTG→GGG) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (32/36);  new base C (5/6);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGA  >  NC_000913/3790745‑3791182
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                      
ggCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCCGCACATCTTCGCCCACCAGATCAAACGAAAGCCCGGTCGGCACGGCGTTACCGCAGGGATCAAAACTTCCGGCTAAGTCATCGGAAATATTGCCGGGGATGTTGAAGGCTCTGAATGCCGGGATCCCCaa                                                                                                                                                                                                                    >  2:672111/1‑228 (MQ=255)
            tAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGGTACCGAAGGGATCAAAAATTGCGGCTAAGGCATCGGAAATATTGTCCGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGAAATTCCGCaaa                                                                                                                                                                                                        >  1:113033/1‑229 (MQ=255)
                      gacccccacAATGCGGCACCACACGTGTTCCCCCACCGCCGCTGCCATAATGCCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCggg                                                                                                                                                                                              <  1:282649/224‑1 (MQ=255)
                          accacATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAAAATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATAACCAGATATTCGGCAAGGCAGCCCGGGGGa                                                                                                                                                                                           >  2:634413/1‑228 (MQ=255)
                            cacaTTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGttt                                                                                                                                                                                         >  1:665355/1‑228 (MQ=255)
                                 tGCGTGCACCGACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCCCATCTTCGCCCACCAGATCAAACGCCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTATCGGGGATTTTGAAGGGGTTGAATCACGGGATCCCCAGATATTCAGCAAAACAGCCCGGGCGaaaaaaaca                                                                                                                                                                                   >  2:251101/1‑221 (MQ=255)
                                 tGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGCAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGGCCTCGGAAATATTGTCGGGGATTTTGAAGACGTTGAATGCAGGGTTAACCAGATATTAGGCAAGACAACCCGGGCGATTAACAcc                                                                                                                                                                                  >  2:71068/1‑229 (MQ=255)
                                  gtgTGCACCAACGTGTTCGCCCACCGCCGCTGCCATAATTCCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACAcc                                                                                                                                                                                  <  1:422188/226‑1 (MQ=255)
                                  gccgcccccAACTTTTTCCGCCCCCGCCGCTGCCATAATCCCAATCGGGCCTGCACCAGAAACCAGCACATCGTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACAcc                                                                                                                                                                                  <  1:65756/221‑1 (MQ=255)
                                      gCACCAGCGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCCAAGTCATCGGAAATAGTGTCGGGGTGTTTGAAGGCGTTGAATGCCGGGATCACCCGATATTCGGCAAAGCAGCCCGGGCGGTTAACACCAACGc                                                                                                                                                                             >  2:179518/1‑229 (MQ=255)
                                      gCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCTGGGATCACCAACAATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGc                                                                                                                                                                             >  2:257098/1‑229 (MQ=255)
                                      gCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCAACCAGATCAAACGACAGCGCGGTATGCACGGCGCTACCGAAGGGATCAAAAATTGCGCCTAAGTCATCGGAAATATGGTCGGGGGTTTTGAAGGCGTTGAATGCCGGGACCCCCAGAATTTCGGCAAAGACGCCCGGGGGATAAAACCCACGcc                                                                                                                                                                            >  2:691773/1‑229 (MQ=255)
                                              cGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGACCCCCAGATATTCGGCAAAGCAGCCCGGGCGATAAACACCacaccccacacg                                                                                                                                                                      >  2:311789/1‑218 (MQ=255)
                                                   tccGCCCCCCCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTg                                                                                                                                                                  <  1:22610/226‑1 (MQ=255)
                                                         cccgccgCTGCCATAATCCCAATCGGGCCTGCCGCAGAAACCAGCACATCTTCGACCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGca                                                                                                                                                             <  1:19640/226‑1 (MQ=255)
                                                          ccgccgCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGccca                                                                                                                                                           >  1:549270/1‑228 (MQ=255)
                                                               gcTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATggg                                                                                                                                                     <  1:614922/229‑1 (MQ=255)
                                                                 tGCCAAAATACCAATCGGGCCTGCACCAGAAACCAGCACATCGTCGCCCAACAGATCAAACGACAGCGAGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTa                                                                                                                                                   <  1:38968/229‑1 (MQ=255)
                                                                   ccttaataCCAACTGGGCCGGCACCCGAAACCTGGCCATTTTCGCCCACCAAATCAAACGCCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACg                                                                                                                                                 <  2:102392/226‑1 (MQ=255)
                                                                     taaataCCAAACGGGCCTGCACCAGAAACCAGCCCATCTTCCCCCCCCAGAACAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGac                                                                                                                                               <  2:549270/227‑1 (MQ=255)
                                                                      taataCCAATCGGGCGTGCCCCAGAAACCCGGACATCTTCGCCCACCGCATCAAACGGCAGCGCGGTATGCCCGGCGTTGCCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATGTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc                                                                                                                                              <  2:474978/229‑1 (MQ=255)
                                                                        agaCCAATCGGGCCTGCTCCAGAAACCAGCACGTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTAATCGGAACTACTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATGaa                                                                                                                                                                                      <  1:80311/185‑1 (MQ=255)
                                                                             aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGCAGGGAACAAAAATTGCCGCTAAGTCCTCGGACAAATTCTCGGGGATTTTGCAGGCTTTGAATGCGGGGATCACCAGCAATTAGGCGAACATGCCCAGGCGATTAAAACCAACCACTATCGCGTTGCGGCACAAAATGGAACGacaccccaga                                                                                                                                       >  2:254809/1‑221 (MQ=255)
                                                                             aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCCATCGTTTTGCGGCACAAATGGGTACGACCACCACGa                                                                                                                                       >  1:180510/1‑229 (MQ=255)
                                                                             aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAAAACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGa                                                                                                                                       >  1:250931/1‑229 (MQ=255)
                                                                             aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGACAACCAAATATTCGGCAAAGCAGCCCGGGCGATTACACCCCAGCCCTAACGAGTTCCGGTCCAAAGGGGTCCAAACACCACgc                                                                                                                                       >  2:4127/1‑228 (MQ=255)
                                                                              aTCGGGCCTGCACCAGAAACCCACACATCTGCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGa                                                                                                                                       <  1:311789/228‑1 (MQ=255)
                                                                              aTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGCTTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCCACGCCCAACGGGTTGCGGCACAAAAGGGAACAGCCCCCACGaa                                                                                                                                      >  2:717049/1‑228 (MQ=255)
                                                                              aTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCCCAAAAGGGTACGACCACCACGAc                                                                                                                                      >  1:412536/1‑229 (MQ=255)
                                                                                        cACCAGTACCCAGCACAAGTTCGCCCACCAGAGCAAACGGCAGCGGGGTATGCACGGCTTTGCCGAAGGGCTCAAAAATTTCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa                                                                                                                            <  2:180510/229‑1 (MQ=255)
                                                                                         agacagaaCCAGGACATGTTCGCCCAGCAGATCAAACGACAGCGCGGTATGCACGGGGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAAGATTTGCGGGGATTTTGAAGGCGTGGAAGGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTAGCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa                                                                                                                            <  1:317937/222‑1 (MQ=255)
                                                                                          ccAGAAACCAGAACAGCTTCGCCCAACAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAAt                                                                                                                          <  1:257098/229‑1 (MQ=255)
                                                                                           cAGAAACCGGCACATATTCGCCGCACAGAACAAACGACAGGGCGGTATGCATGGCGGTACCGAAGGGATTAAAAATTGCGGCTAAGTCATCGGAAACATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATGCGGCAAAGCAGCCCGGGCGATTACCACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa                                                                                                                            <  1:347501/226‑1 (MQ=255)
                                                                                             gAAACAAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAc                                                                                                                       >  1:132341/1‑229 (MQ=255)
                                                                                               aaCCCGCCCATCGTTGCCCACCAGAATAAACGGCAGGGCGGGATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTATGACCACCACGACAGTTGCGGCAATGAccc                                                                                                                     <  2:620911/229‑2 (MQ=255)
                                                                                               aaCCAGCCCATCTGCGCCCACCAGAGCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATAGTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAcc                                                                                                                      <  1:282689/228‑1 (MQ=255)
                                                                                                aCCAGCCCCTCTTTCGCCACCAGAAAAAACGGCCGCGGGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGGCAGCGGAAATATTGTCGGGGATTTTGCAGGCGTTGAATGCCTGGATCACCAGATATTCGGCCAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGTCCACCACGACAGTTGCGGCAATGACcac                                                                                                                    <  2:17668/229‑1 (MQ=255)
                                                                                                  cAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAAGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCCGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTAAGACCACCCAGCAAGTTGCGGCCATGccccac                                                                                                                   >  2:196777/1‑225 (MQ=255)
                                                                                                     cacaTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCCAAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGt                                                                                                                >  1:157622/1‑228 (MQ=255)
                                                                                                     cacaTCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAggg                                                                                                                >  1:539434/1‑227 (MQ=255)
                                                                                                         tCTGCGCCCACTAGATCAAACCAACAGGCGGATGGGCGGGCGTTAACGAGTGGATCAAAAATTGCGGCTAAGTCATTGGAAAAATTGTCGGGGATTTTGAAGGGGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGTGATTAACACCAACGCATATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGata                                                                                                            <  2:469957/228‑1 (MQ=255)
                                                                                                          cTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCCCGACAATTGCGGCAATGACCACAGGGGATATg                                                                                                          >  1:751286/1‑229 (MQ=255)
                                                                                                             cGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTACCGTGTTGCGGCACAAATGGGGACGACCACCACGACAGTTGCGGCAATGACCCCAGGTGTAATGGc                                                                                                        >  1:420325/1‑228 (MQ=255)
                                                                                                                ccaccaGATCAAACGGCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGcc                                                                                                       <  1:605679/226‑1 (MQ=255)
                                                                                                                  accaGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCg                                                                                                   >  1:530931/1‑228 (MQ=255)
                                                                                                                       aTCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCgg                                                                                                                                                               >  2:594908/1‑163 (MQ=255)
                                                                                                                       aTCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCgg                                                                                                                                                               <  1:594909/163‑1 (MQ=255)
                                                                                                                            aCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGCCCCAAGGTGATATGGCCTTCTCCAGAAAcg                                                                                          >  1:273610/1‑227 (MQ=255)
                                                                                                                               gACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGGACGACCACCACGACAGTTGCGGCAATGACCACAGGGGATATGGCCTTCGCCAGAAACGCGac                                                                                      >  1:413795/1‑228 (MQ=255)
                                                                                                                                acggtgcgGTATGGACGGGTTTACCGGAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGTATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATc                                                                                     <  2:157622/224‑1 (MQ=255)
                                                                                                                                  cgcggtgTAAGCACGGGGGTCCCGGAGGAACTAAAAATTGGGGCTAAGTCATCGGAAAAATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCAAAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGc                                                                                   <  2:438173/223‑1 (MQ=255)
                                                                                                                                  aGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGCACAACACGACAATTGCGGCAATGCACACAGGCGATATGGCCTTCGCCAGAACCGCGATCGc                                                                                   >  1:352275/1‑229 (MQ=255)
                                                                                                                                    cggggTTTGCACGGTTTTACCGAAGGGATAAAAAATTGGGGGTAAGTCACCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCGTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCg                                                                                 <  2:113033/226‑1 (MQ=255)
                                                                                                                                     cgggTATGCAGGGCGTTAACGAAGGGATCAAAAATTGCGGCGAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATAGTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGGACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGcc                                                                                  <  1:283524/225‑1 (MQ=255)
                                                                                                                                         tATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACCACAGTTGCGGCAATGACCCCAGGGGATATGGCCTTCGCCAGAAACGCGAACCCCGAACtt                                                                            >  2:271951/1‑229 (MQ=255)
                                                                                                                                          aTGCACGGCTTTACCGAAGGGATCAAAAATTGCGGCTAATTCAGCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTg                                                                           <  1:373302/229‑1 (MQ=255)
                                                                                                                                               cGGCGTTACCGGAGGGATCAAAAATTGCGGCTAAGTCAGCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCAACAGATATTCGGCAAAGCAGGCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGc                                                                       <  1:270889/228‑1 (MQ=255)
                                                                                                                                                    gTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACAACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGGTCTTGAAGCCTTTc                                                                  >  1:375964/1‑229 (MQ=255)
                                                                                                                                                      tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTCCGACCACCACGACAGTTGCGGCCATGACCAAAGGAGATATGGCATCCCCCAGAAACCCGATCGCCAGTCTTACACCCtttaca                                                                >  2:657372/1‑226 (MQ=255)
                                                                                                                                                        ccGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCGATCGTGTTGCGGCACAAATGGGTAGGACCACCACCAAAGTTGCGGGAATGACCCCAGGGGATATGGCCTTCGCCAGCAACACGATCCCCGACTTTCAACCCTTTCACtt                                                              >  2:196790/1‑229 (MQ=255)
                                                                                                                                                             gggATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGGAAAGCAGCCCGGGCGCTTAACACCAACGACTATCGTGTTTAGGTACAAATGGGGCAGACCACCACGACAGTTGCGGCGATGACCACAGGGGACAGGCGCTTCGCCCGAAACCGAATCGCCGAACTGCAAGACTTTCACTTCCTg                                                          >  2:293377/1‑228 (MQ=255)
                                                                                                                                                              gggtCAAAAATTGCGGTTAAGCCACCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAAGGCCGGGACCACCAGATATTTGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTGTCGTGTTGCGGCACAAAGGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc                                                        <  2:665354/226‑1 (MQ=255)
                                                                                                                                                               tgtaaaaaaTCGCGGCTCAGTTGTAGGAAATATTGGCGGGGATTTTGAAGGGTGTAAAAGCCGGGATCCCCAGATATTCGGGAAGTCATCCCGGTCGAGTAACACCAACGCCTATCGTGTTGCGGCATAAATGGGTACGCCCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTGCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc                                                        <  2:153723/224‑1 (MQ=255)
                                                                                                                                                               gATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc                                                        <  1:251101/228‑1 (MQ=255)
                                                                                                                                                               gATCAAAAATTGCGGCAAAGTCATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTTCGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGTCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAcc                                                       <  1:177452/229‑1 (MQ=255)
                                                                                                                                                               gATAAAAAATTGCGGTTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGACCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCAGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc                                                        <  1:71068/228‑1 (MQ=255)
                                                                                                                                                                attaaaaaTTGCGGGTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATAGTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTg                                                                           <  1:284649/205‑1 (MQ=255)
                                                                                                                                                                aTCAAAAATTGCGGCTAAGTCATCGGAAATTTGGTCGGGGATTTTGAAGGCGTGGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCa                                                      <  1:420146/229‑1 (MQ=255)
                                                                                                                                                                aTCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACCGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCCGAAACACGATCGCCGATCTTAAAGCCTTTAAccgttctctta                                                      >  2:284649/1‑219 (MQ=255)
                                                                                                                                                                         tGCGGCAAAGTCATCGGAAATATTGTGGGGGATTTTGAAGGGTTTGAAGGCCGGGCCCCCCAGATTTTCGGCAAAGTAGCCCGGGGCATTAACACCAACGCCTATCGTTTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtacctac                                              <  2:539434/228‑1 (MQ=255)
                                                                                                                                                                               ttagttacccgAAATTTTGTGGGGGATTTCGGAGGGGTTGAAGGCCGGGATCCCGAGGTATTCGGTAATGCAGCCCTGGCGATTCACATCAACGCCTACCGTGTCGCGGCACAAATGGGTACGACCACCGCGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCCCTTCa                                       <  2:420325/219‑1 (MQ=255)
                                                                                                                                                                                 aGTCAGCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCAcc                                     <  1:4127/229‑1 (MQ=255)
                                                                                                                                                                                   tCATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGa                                   <  1:634414/229‑1 (MQ=255)
                                                                                                                                                                                    cATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGAc                                  <  1:271951/229‑1 (MQ=255)
                                                                                                                                                                                      gcGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACa                                 <  1:717050/227‑1 (MQ=255)
                                                                                                                                                                                                            tGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGCTGCGGCAATGACCACAGGTGATATGGCCTTCGCCCGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATACTCATGGCCCACACCCATCgg          >  2:645733/1‑229 (MQ=255)
                                                                                                                                                                                                                  cGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGAc                                  >  1:259105/1‑199 (MQ=255)
                                                                                                                                                                                                                   gTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTTTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCggg   <  1:489028/229‑1 (MQ=255)
                                                                                                                                                                                                                    ttGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCACTACCTACCACTTCACCGCCATATTCATGGCCCCCGACCCTCGACACCgggg  >  2:477981/1‑228 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                      
GGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGA  >  NC_000913/3790745‑3791182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: