Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,790,969 | A→C | 10.0% | V126G (GTG→GGG) | tdh ← | L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,790,969 | 0 | A | C | 10.0% | 193.7 / 2.3 | 79 | V126G (GTG→GGG) | tdh | L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
Reads supporting (aligned to +/- strand): ref base A (32/36); new base C (5/6); total (37/42) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGA > NC_000913/3790745‑3791182 | ggCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCCGCACATCTTCGCCCACCAGATCAAA‑CGAAAGCCCGGTCGGCAC‑GGCGTTACCGCAGGGATCAAAACTTCCGGCTAAGTCATCGGAAATATTGCCGGGGATGTTGAAGGCTCTGAATGCCGGGATCCCCaa > 2:672111/1‑228 (MQ=255) tAACATCAGTGATCACCACATTGCGTGCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGGTACCGAAGGGATCAAAAATTGCGGCTAAGGCATCGGAAATATTGTCCGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGAAATTCCGCaaa > 1:113033/1‑229 (MQ=255) gacccccacAATGCG‑GCACCACACGTGTTCCCCCACCGCCGCTGCCATAATGCCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCggg < 1:282649/224‑1 (MQ=255) accacATTGCGTGCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAAAATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATAACCAGATATTCGGCAAGGCAGCCCGGGGGa > 2:634413/1‑228 (MQ=255) cacaTTGCGTGCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGttt > 1:665355/1‑228 (MQ=255) tGCGTGCACCG‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCCCATCTTCGCCCACCAGATCAAA‑CGCCAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTATCGGGGATTTTGAAGGGGTTGAATCACGGGATCCCCAGATATTCAGCAAAACAGCCCGGGCGaaaaaaaca > 2:251101/1‑221 (MQ=255) tGCGTGCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGCAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGGCCTCGGAAATATTGTCGGGGATTTTGAAGACGTTGAATGCAGGGTTAACCAGATATTAGGCAAGACAACCCGGGCGATTA‑ACAcc > 2:71068/1‑229 (MQ=255) gtgTGCACCA‑ACGTGTTCGCCCACCGCCGCTGCCATAATTCCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACAcc < 1:422188/226‑1 (MQ=255) gccgcccccA‑ACTTTTTCCGCCCCCGCCGCTGCCATAATCCCAATCGGGCCTGCACCAGAAACCAGCACATCGTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACAcc < 1:65756/221‑1 (MQ=255) gCACCA‑GCGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCCAAGTCATCGGAAATAGTGTCGGGGTGTTTGAAGGCGTTGAATGCCGGGATCACCCGATATTCGGCAAAGCAGCCCGGGCGGTTA‑ACACCAACGc > 2:179518/1‑229 (MQ=255) gCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCTGGGATCACCAACAATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGc > 2:257098/1‑229 (MQ=255) gCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCAACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGCTACCGAAGGGATCAAAAATTGCGCCTAAGTCATCGGAAATATGGTCGGGGGTTTTGAAGGCGTTGAATGCCGGGACCCCCAGAATTTCGGCAAAGACGCCCGGGGGA‑TA‑AAACCCACGcc > 2:691773/1‑229 (MQ=255) cGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGACCCCCAGATATTCGGCAAAGCAGCCCGGGCGATAA‑ACACCacaccccacacg > 2:311789/1‑218 (MQ=255) tccGCCCCCCCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTg < 1:22610/226‑1 (MQ=255) cccgccgCTGCCATAATCCCAATCGGGCCTGCCGCAGAAACCAGCACATCTTCGACCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGca < 1:19640/226‑1 (MQ=255) ccgccgCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGccca > 1:549270/1‑228 (MQ=255) gcTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATggg < 1:614922/229‑1 (MQ=255) tGCCAAAATACCAATCGGGCCTGCACCAGAAACCAGCACATCGTCGCCCAACAGATCAAA‑CGACAGCGAGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTa < 1:38968/229‑1 (MQ=255) ccttaataCCAACTGGGCCGGCACCCGAAACCTGGCCATTTTCGCCCACCAAATCAAA‑CGCCAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACg < 2:102392/226‑1 (MQ=255) taaataCCAAACGGGCCTGCACCAGAAACCAGCCCATCTTCCCCCCCCAGAACAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGac < 2:549270/227‑1 (MQ=255) taataCCAATCGGGCGTGCCCCAGAAACCCGGACATCTTCGCCCACCGCATCAAA‑CGGCAGCGCGGTATGCCC‑GGCGTTGCCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATGTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc < 2:474978/229‑1 (MQ=255) agaCCAATCGGGCCTGCTCCAGAAACCAGCACGTCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTAATCGGAACTACTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATGa‑a < 1:80311/185‑1 (MQ=255) aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGCAGGGAACAAAAATTGCCGCTAAGTCCTCGGACAAATTCTCGGGGATTTTGCAGGCTTTGAATGCGGGGATCACCAGCAATTAGGCGAACATGCCCAGGCGATTA‑AAACCAACCACTATCGCGTTGCGGCACAAAATGGAACGacaccccaga > 2:254809/1‑221 (MQ=255) aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCCATCGTTTTGCGGCACAAATGGGTACGACCACCACGa > 1:180510/1‑229 (MQ=255) aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑AAACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGa > 1:250931/1‑229 (MQ=255) aaTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGACAACCAAATATTCGGCAAAGCAGCCCGGGCGATTACACCCCAGC‑CCTAACGAGTTCCGGTCCAAAGGGGTCCAAACACCACgc > 2:4127/1‑228 (MQ=255) aTCGGGCCTGCACCAGAAACCCACACATCTGCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGa < 1:311789/228‑1 (MQ=255) aTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGCTTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCCACGCCCAACGGGTTGCGGCACAAAAGGGAACAGCCCCCACGaa > 2:717049/1‑228 (MQ=255) aTCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCCCAAAAGGGTACGACCACCACGAc > 1:412536/1‑229 (MQ=255) cACCAGTACCCAGCACAAGTTCGCCCACCAGAGCAAA‑CGGCAGCGGGGTATGCAC‑GGCTTTGCCGAAGGGCTCAAAAATTTCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa < 2:180510/229‑1 (MQ=255) agacagaaCCAGGACATGTTCGCCCAGCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGGGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAAGATTTGCGGGGATTTTGAAGGCGTGGAAGGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTAGCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa < 1:317937/222‑1 (MQ=255) ccAGAAACCAGAACAGCTTCGCCCAACAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAAt < 1:257098/229‑1 (MQ=255) cAGAAACCGGCACATATTCGCCGCACAGAACAAA‑CGACAGGGCGGTATGCAT‑GGCGGTACCGAAGGGATTAAAAATTGCGGCTAAGTCATCGGAAACATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATGCGGCAAAGCAGCCCGGGCGATTA‑CCACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCa < 1:347501/226‑1 (MQ=255) gAAACAAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAc > 1:132341/1‑229 (MQ=255) aaCCCGCCCATCGTTGCCCACCAGAATAAA‑CGGCAGGGCGGGATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTATGACCACCACGACAGTTGCGGCAATGAccc < 2:620911/229‑2 (MQ=255) aaCCAGCCCATCTGCGCCCACCAGAGCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATAGTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAcc < 1:282689/228‑1 (MQ=255) aCCAGCCCCTCTTTCGCCACCAGAAAAAA‑CGGCCGCGGGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGGCAGCGGAAATATTGTCGGGGATTTTGCAGGCGTTGAATGCCTGGATCACCAGATATTCGGCCAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGTCCACCACGACAGTTGCGGCAATGACcac < 2:17668/229‑1 (MQ=255) cAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAAGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCCGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTAAGACCACCCAGCAAGTTGCGGCCATGccccac > 2:196777/1‑225 (MQ=255) cacaTCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCCAAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGt > 1:157622/1‑228 (MQ=255) cacaTCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAggg > 1:539434/1‑227 (MQ=255) tCTGCGCCCACTAGATCAAACCAACAG‑GCGG‑ATGGGCGGGCGTTAACGAGTGGATCAAAAATTGCGGCTAAGTCATTGGAAAAATTGTCGGGGATTTTGAAGGGGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGTGATTA‑ACACCAACGCATATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGata < 2:469957/228‑1 (MQ=255) cTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCCCGACAATTGCGGCAATGACCACAGGGGATATg > 1:751286/1‑229 (MQ=255) cGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTACCGTGTTGCGGCACAAATGGGGACGACCACCACGACAGTTGCGGCAATGACCCCAGGTGTAATGGc > 1:420325/1‑228 (MQ=255) ccaccaGATCAAA‑CGGCAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGcc < 1:605679/226‑1 (MQ=255) accaGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCg > 1:530931/1‑228 (MQ=255) aTCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCgg > 2:594908/1‑163 (MQ=255) aTCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCgg < 1:594909/163‑1 (MQ=255) a‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGCCCCAAGGTGATATGGCCTTCTCCAGAAAcg > 1:273610/1‑227 (MQ=255) gACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGGACGACCACCACGACAGTTGCGGCAATGACCACAGGGGATATGGCCTTCGCCAGAAACGCGac > 1:413795/1‑228 (MQ=255) acggtgcgGTATGGAC‑GGGTTTACCGGAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGTATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATc < 2:157622/224‑1 (MQ=255) cgcggtgTAAGCAC‑GGGGGTCCCGGAGGAACTAAAAATTGGGGCTAAGTCATCGGAAAAATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCAAAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGc < 2:438173/223‑1 (MQ=255) aGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGCACAACACGACAATTGCGGCAATGCACACAGGCGATATGGCCTTCGCCAGAACCGCGATCGc > 1:352275/1‑229 (MQ=255) cggggTTTGCAC‑GGTTTTACCGAAGGGATAAAAAATTGGGGGTAAGTCACCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCGTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCg < 2:113033/226‑1 (MQ=255) cgggTATGCAG‑GGCGTTAACGAAGGGATCAAAAATTGCGGCGAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATAGTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGGACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGcc < 1:283524/225‑1 (MQ=255) tATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACCACAGTTGCGGCAATGACCCCAGGGGATATGGCCTTCGCCAGAAACGCGAACCCCGAACtt > 2:271951/1‑229 (MQ=255) aTGCAC‑GGCTTTACCGAAGGGATCAAAAATTGCGGCTAATTCAGCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTg < 1:373302/229‑1 (MQ=255) c‑GGCGTTACCGGAGGGATCAAAAATTGCGGCTAAGTCAGCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCAACAGATATTCGGCAAAGCAGGCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGc < 1:270889/228‑1 (MQ=255) gTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACAACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGGTCTTGAAGCCTTTc > 1:375964/1‑229 (MQ=255) tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTCCGACCACCACGACAGTTGCGGCCATGACCAAAGGAGATATGGCATCCCCCAGAAACCCGATCGCCAGTCTTACACCCtttaca > 2:657372/1‑226 (MQ=255) ccGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCGATCGTGTTGCGGCACAAATGGGTAGGACCACCACCAAAGTTGCGGGAATGACCCCAGGGGATATGGCCTTCGCCAGCAACACGATCCCCGACTTTCAACCCTTTCACtt > 2:196790/1‑229 (MQ=255) gggATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGGAAAGCAGCCCGGGCGCTTA‑ACACCAACGACTATCGTGTTTAGGTACAAATGGGGCAGACCACCACGACAGTTGCGGCGATGACCACAGGGGACAGGCGCTTCGCCCGAAACCGAATCGCCGAACTGCAAGACTTTCACTTCCTg > 2:293377/1‑228 (MQ=255) gggtCAAAAATTGCGGTTAAGCCACCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAAGGCCGGGACCACCAGATATTTGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTGTCGTGTTGCGGCACAAAGGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc < 2:665354/226‑1 (MQ=255) tgtaaaaaaTCGCGGCTCAGTTGTAGGAAATATTGGCGGGGATTTTGAAGGGTGTAAAAGCCGGGATCCCCAGATATTCGGGAAGTCATCCCGGTCGAGTA‑ACACCAACGCCTATCGTGTTGCGGCATAAATGGGTACGCCCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTGCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc < 2:153723/224‑1 (MQ=255) gATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc < 1:251101/228‑1 (MQ=255) gATCAAAAATTGCGGCAAAGTCATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTTCGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGTCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAcc < 1:177452/229‑1 (MQ=255) gATAAAAAATTGCGGTTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGACCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCAGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc < 1:71068/228‑1 (MQ=255) attaaaaaTTGCGGGTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATAGTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTg < 1:284649/205‑1 (MQ=255) aTCAAAAATTGCGGCTAAGTCATCGGAAATTTGGTCGGGGATTTTGAAGGCGTGGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCa < 1:420146/229‑1 (MQ=255) aTCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACCGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCCGAAACACGATCGCCGATCTTAAAGCCTTTAAccgttctctta > 2:284649/1‑219 (MQ=255) tGCGGCAAAGTCATCGGAAATATTGTGGGGGATTTTGAAGGGTTTGAAGGCCGGGCCCCCCAGATTTTCGGCAAAGTAGCCCGGGGCATTA‑ACACCAACGCCTATCGTTTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtacctac < 2:539434/228‑1 (MQ=255) ttagttacccgAAATTTTGTGGGGGATTTCGGAGGGGTTGAAGGCCGGGATCCCGAGGTATTCGGTAATGCAGCCCTGGCGATTC‑ACATCAACGCCTACCGTGTCGCGGCACAAATGGGTACGACCACCGCGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCCCTTCa < 2:420325/219‑1 (MQ=255) aGTCAGCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCAcc < 1:4127/229‑1 (MQ=255) tCATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCCCCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGa < 1:634414/229‑1 (MQ=255) cATCGGAAATATTGGCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGAc < 1:271951/229‑1 (MQ=255) gcGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACa < 1:717050/227‑1 (MQ=255) tGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGCTGCGGCAATGACCACAGGTGATATGGCCTTCGCCCGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATACTCATGGCCCACACCCATCgg > 2:645733/1‑229 (MQ=255) cGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGAc > 1:259105/1‑199 (MQ=255) gTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTTTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCggg < 1:489028/229‑1 (MQ=255) ttGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCACTACCTACCACTTCACCGCCATATTCATGGCCCCCGACCCTCGACACCgggg > 2:477981/1‑228 (MQ=255) | GGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCA‑ACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAA‑CGACAGCGCGGTATGCAC‑GGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTA‑ACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGA > NC_000913/3790745‑3791182 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |