Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,253,517 A→G 11.9% S168G (AGT→GGT)  ubiA → p‑hydroxybenzoate octaprenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,253,5170AG11.9% 175.5 / 2.5 77S168G (AGT→GGT) ubiAp‑hydroxybenzoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base A (33/32);  new base G (6/5);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTG  >  NC_000913/4253297‑4253737
                                                                                                                                                                                                                             |                                                                                                                                                                                                                            
agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGCAGCGGCATACCCATCTACCGCAAGTGGGCCTGGGCGCGGCGTTTGGGTGGTCGGTTCCAATGGCTTTTGCCCCTGTGGGGGAGTCGGGCCCGTTGGGTTGCTg                                                                                                                                                                                                                       >  2:344968/1‑228 (MQ=255)
agagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGGGAGTGAGTCGGTGCCTTTGGGTTGCt                                                                                                                                                                                                                        >  1:734462/1‑227 (MQ=255)
 gagGCGCGCGCGCTAGTTGTCGTGCTGGTACTGATTTCGTTTTTGCTGGTGCTGACGCTGAATTCGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTTGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTgg                                                                                                                                                                                                                      <  1:112348/228‑1 (MQ=255)
      gcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGGGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGCCTTTTGCCGCTGTGAGTGAGTCGGGGCCATTGAGTTGCTGGGTAATg                                                                                                                                                                                                                >  1:390506/1‑229 (MQ=255)
        gcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGGGAGGGAGTCGGGGCCGTTGAGTTGCTGGTTATTGtt                                                                                                                                                                                                              >  1:330568/1‑229 (MQ=255)
         gtggttttttGTCGTGCTGGTTCTGTTTTCGTTTTTACTGGTGCTGACGCTGAATGCGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTGATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTc                                                                                                                                                                                                             <  2:208752/222‑1 (MQ=255)
             ctttttGTCGTTCTGGTTCTGATTTCGGTTTTCCTGGTGCTGACGGTGAATACGGTGACCATTTTGTTGTCGATTGCCGCGCTAGCGCTGGCCTGGGTGTTCCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCt                                                                                                                                                                                                           <  2:501778/224‑1 (MQ=255)
                          tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTCTACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGGGAGTCGGTGCCATTGCGTTGCTGGTTAATGTTCCTCGCCCATATTCTCTg                                                                                                                                                                                            >  1:180824/1‑229 (MQ=255)
                          tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTg                                                                                                                                                                                            >  1:63585/1‑229 (MQ=255)
                          tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGCGAGTCGGTGCCATTGAGGTGCGGGTTAATGTTCTTAGCCAAAATTCtgtg                                                                                                                                                                                            >  1:641884/1‑229 (MQ=255)
                                  aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGGGAGGGGGTCGGTGCCCTTGGGTTGCTGGGTAATGTTCCTCGCCAATATTTTCTGGGCGgggg                                                                                                                                                                                    >  2:162532/1‑229 (MQ=255)
                                   ttttGTTTTTGCGGGTGCTGGCGCTGAATACGATGCCCTTTCTTTGTGCGGTTGCCGCGCTGGGGCTGGCGGGGGTGTTCCCGTTTATGAAGCGGTATACCAATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGc                                                                                                                                                                                   <  2:603654/229‑1 (MQ=255)
                                    ttgttttttACGGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTTCCCGTTTATGAAGCGGTATCCCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGc                                                                                                                                                                                   <  1:248429/224‑1 (MQ=255)
                                      gttttttACTGGGGCTGACTGTGAAGACGATTCCCATTCTGTTTGCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTa                                                                                                                                                                                <  2:526141/227‑1 (MQ=255)
                                       gTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTCGCGCTCGCGTGGGTGTACCCGTTTATTAAGCGGTATACCCATCTACCGCACGGGGTGCTGGGCGCGCCGTTTGGCTGTTCGATTCCAATGCCTTTTGCCGCTGTTATTGGGTCGTCGCCCTTTGGTTTCCGGTTATTGTTCCTCCCCAATATTTCTTGGGGGGTGGCttcc                                                                                                                                                                               >  2:355827/1‑227 (MQ=255)
                                       gTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCCCTTTGAGGGGGTCGGGGCCATTGAGTTGCTGGTTATGGTTCCTCGCCATTATTTCCTGGGCGGTGGGTTAc                                                                                                                                                                               >  1:50339/1‑229 (MQ=255)
                                       gTTTTTACTGGGGCTGACGCTGAAGACGATTACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGCTTATGAAGCGGTATACCCATCTACCGCAAGGGGGGCTGGGGGCGGCGTTTGGCTGGTCGCTTTCAATGCCTTTTGACGCTGTGAGTGAGTCGGGTCCATTGAGTTGCTGGTTAATTTTCCTCCCCCGTATTCTCTGGGCGTTGGCTTAc                                                                                                                                                                               >  2:673373/1‑229 (MQ=255)
                                            tACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGc                                                                                                                                                                                   >  1:603655/1‑220 (MQ=255)
                                            tACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGGGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGGTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGacac                                                                                                                                                                          >  1:559957/1‑229 (MQ=255)
                                             aCTGGTGCTGACGCTGAATTCGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACg                                                                                                                                                                         <  1:489341/229‑1 (MQ=255)
                                                ggTGCTGACGGTGAATCCGTTGACCATTCTGTTGGCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAg                                                                                                                                                                      <  1:462934/229‑1 (MQ=255)
                                                            gAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAAGGTTCTTCGCCATTATTCTCTGGGCGGTGGCTTACGCCACGCAGTATGCGATGGtt                                                                                                                                                          >  1:193942/1‑229 (MQ=255)
                                                                   aTGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGAACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCACTGGCTTTTGCCGATGTGAGTGAGTCGGTGCCATTGATTTGTTGGTTCATGTTCCTCGCCCATATTCTCTGGGCGGTGGCTTACGACACCCCGTATGCGATGGTTGACcggc                                                                                                                                                   >  2:515043/1‑227 (MQ=255)
                                                                     gACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGGGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGTTTACGCCACGCAGTATGCGATTGTTGACCGCgat                                                                                                                                                 >  1:414657/1‑229 (MQ=255)
                                                                      aCCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatg                                                                                                                                                <  1:230591/229‑1 (MQ=255)
                                                                       cctttgtgttgtCTATTGCCGCGCTTGCCCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatg                                                                                                                                                <  2:63585/222‑1 (MQ=255)
                                                                       ccATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGGGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTTGCCAATATTCTCTGGGCGGAGGCTTACGACCCGCAGTATGCGATGGTTGACCGCgctga                                                                                                                                               >  1:48973/1‑229 (MQ=255)
                                                                        cATGCTGTTGGCTGTTGCCGCGCTTGCCGTGGCGTGGGTTTACCCGTTTATGAAGCGGTATACCCATCTACCGGAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatgat                                                                                                                                              <  1:642392/229‑1 (MQ=255)
                                                                          ttCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACAGGCAGAATGCGATGGTTGACCGCgatgatga                                                                                                                                            >  1:429822/1‑229 (MQ=255)
                                                                           tCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCACGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGGGTTAGTCGGTGCCATTGGGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTAGGAGCGCCATTATGCAGTGGGTGACcggctgggtggg                                                                                                                                           >  2:542059/1‑219 (MQ=255)
                                                                             ttttgtCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGGAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGAtgt                                                                                                                                         <  1:686838/227‑1 (MQ=255)
                                                                                  tCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGa                                                                                                                                    >  1:362545/1‑229 (MQ=255)
                                                                                                 aGCGCTGGCGTGGGTGTCCCCGTTTATGAAGCGGTATTCCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATc                                                                                                                       <  1:162532/228‑1 (MQ=255)
                                                                                                  gcggTGGCGGGGGTTTCCCCGTTTCTGAAGCGGTTTACCTATCTACCTCAAGTGGTGCTGGGGGGGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCa                                                                                                                     <  2:550666/229‑1 (MQ=255)
                                                                                                        gCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCACTATTCTCTGGGCGGTGGCTTACGACACGCAGAATGCGATGGTTTACCGCGAGATTGTAGTGAAGATTGCAATTCAATCCCCggga                                                                                                                >  1:504326/1‑226 (MQ=255)
                                                                                                                  aCCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCCTTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGCCACGCAGATTGCGATGGTTGACCGCGGTGCGGGTGGGAAGCTTGGCCTTAATTCAACGGAACTCCTGTTggg                                                                                                     >  2:312789/1‑229 (MQ=255)
                                                                                                                  aCCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCCAAATTCTCTGGGGGGAGGCTTACGACACGCAGTATGCGAGGGTTGACCGCGCTGATGATGAGGAGATTGGCATTAAATCCACGGaaaactgcttggg                                                                                                     >  2:269567/1‑219 (MQ=255)
                                                                                                                  aCCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGCCCGGCAGTATGCGATGGTTGACCGCGTTGGTGAGTGGAAGATTGCCCTTAATTCCACGGCAACCCTTTTggg                                                                                                     >  2:584081/1‑229 (MQ=255)
                                                                                                                  aCCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCgg                                                                                                     >  1:381024/1‑229 (MQ=255)
                                                                                                                      tgttATGAAGCGGGATCCCCATTTCAGGGAGGTGGTGGTGGGCGCGGTGTTTGGCTGGTCGATTCGAAGGGCTTGTGCCGCTGTGAGTGAGTCGGTGCCATTGAGGTGCTGGTTAATGTTCCTCGCCAATAGTCTCTGGGCGGGGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGGAATCCTGTTCGGCCa                                                                                                  <  2:404318/226‑1 (MQ=255)
                                                                                                                      gTTTATGAAGCGGTATACCCATCTACCGCAAGGGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCa                                                                                                  <  1:19398/228‑1 (MQ=255)
                                                                                                                       tttATGAAGCGGTATACCCATCTACCGCAAGGGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGGGTTGCTGGTTAATGTTCCCCGCCAATATTCTCTGGGCGGTGGCTTACGCCACGACGTATGCGATGGTTGACCGCGATGATGATTGGTAGTTTGGCATTAAATCCACGGCAATCCTGTTCGGCCAAt                                                                                                >  2:214657/1‑229 (MQ=255)
                                                                                                                         tATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATAc                                                                                              >  1:510496/1‑229 (MQ=255)
                                                                                                                          aTGGAGCGGTTATCCCATTTACCGCAGGTTGTGCTGGGCGAGGCTTTTGGCCGGTCGAGTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATAc                                                                                              <  2:180824/228‑1 (MQ=255)
                                                                                                                               cgggTATTCCCATTTTCTGGGAGTTGTGTAGGGCGGTGCGTTTGGTTGGTTGATTCCAATGGCTTTTGCCGTTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCATTATTCTAGGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATaaa                                                                                        <  1:673374/227‑1 (MQ=255)
                                                                                                                                 ggTTTACCCATATACCGTAAGTGGGGCTGGTCGCGGGGTTTGGCTGGTCGATTCCAATGGTTTGTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAAt                                                                                       <  1:344968/228‑1 (MQ=255)
                                                                                                                                          aTCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGGCACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCAAGGCAATCCGGTTCGGCCAATACGCTAAATTGATTATggg                                                                             >  1:249879/1‑229 (MQ=255)
                                                                                                                                             tACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACCCGCAGTATGCGCTGGTTGACCGCGATGAGGGTGTTAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGGTAAATTGTTTATTGGGAt                                                                          >  2:599321/1‑229 (MQ=255)
                                                                                                                                             tACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGCGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGGGAAGGTTGGAATTAAATCCACGGAAATCCTGTTCGGCCAAACCGATAAATTGATTATTGGGAt                                                                          >  1:36162/1‑229 (MQ=255)
                                                                                                                                                    aGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGGTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGTTGTGAAGATTGGCATTAAACTCACGGAAATCCTGTTCGGCCAATACGTTAAATTGATTATTGGATTTTTGcga                                                                   >  1:337818/1‑227 (MQ=255)
                                                                                                                                                     gtggtgCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGa                                                                  <  1:373999/229‑1 (MQ=255)
                                                                                                                                                         tgCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGGTCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTTTGGGCGGTGGCTTACGGCACGCAGTATGCGATGGTTGACCGCCATGATGATGTGCAGATTGGCATTAAATCCACGGCAATCCGGTTCGGACAAAACCAAAAATTGATTATTGGGATTTTTCAAATAgg                                                              >  2:283819/1‑229 (MQ=255)
                                                                                                                                                          gCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGGGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTTGGCGGTGGCTTACGACACGAAGTATGCGATGGTTGACCGCGCTGATGATGTGAAAATTGCAATTAAATCCACGGCAATCCTGTTCGGCCAATAAGAGAATTTGTTTCTTGGTATTTTGCAGATTggt                                                             >  2:641641/1‑228 (MQ=255)
                                                                                                                                                           cttggCGTGGGGTTTGGCTGGTTGATTCCAATGGTTTTTGCCGTTGGGAGTGAGTCGGTGCCTTTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATCGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCg                                                            <  2:330568/226‑1 (MQ=255)
                                                                                                                                                           cggggCGCGGCGTTTGGCGGGTCGATGCCAATGGCTTTTGCCGCTGGGAGGGAGTGGGTGCCATTGAGTTGCTGGTGAAGGTTCCTCGCCAATATTCTCGGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCg                                                            <  1:355827/227‑1 (MQ=255)
                                                                                                                                                             gggcggggcgTTTGGCTTGGCGATTCCAATGGCTTTTGCCGTTGTGAGTGAGTCGGGGACATTGGGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTa                                                          <  2:632086/229‑1 (MQ=255)
                                                                                                                                                                  cggcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGc                                                     >  1:653503/1‑229 (MQ=255)
                                                                                                                                                                   ggcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCGGTTTGGCGTACTGGCa                                                    >  1:522662/1‑229 (MQ=255)
                                                                                                                                                                   ggcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGGATTTTGCAGATTGCGGTACTGGCa                                                    >  1:591317/1‑229 (MQ=255)
                                                                                                                                                                    gcgTTTGGATGGTCGATTCGAATGGCTTTTGCCGGTGTGAGTGAGTCGGGGCCATTGGGTTGCTGTTTAATGTTCCTCGCCAATATTCTCGGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCa                                                    <  1:256959/228‑1 (MQ=255)
                                                                                                                                                                                     tccatgggtttttGCCGGTGTGAGGGGTTTGGGGCCATGTGGTTGCTGGTTAATGTTCCCCGTCAATGTTCTCTGGGCGGGGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCAATGATGGCGGTCATCGGt                                  <  2:362545/220‑1 (MQ=255)
                                                                                                                                                                                           ggCTTTTTCCGCTGTGAGTGAGTCGGTGCCTTTGGGTTGCTGGTTAATTTGCCGCGCCAATATTCTCTGGGCGGTGGCTTACGACCCGCAGGATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGtt                             <  2:414657/228‑1 (MQ=255)
                                                                                                                                                                                            ttttttGCCGCTGTGAGGGAGTCGGGGCTATTGAGTTGCTGGTTAATGTTCCTCGCAAATATTCTCTGGGCGGTTGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTaa                           <  2:381024/227‑1 (MQ=255)
                                                                                                                                                                                                 tGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatgat                                                                                                                                              <  1:432396/109‑1 (MQ=255)
                                                                                                                                                                                                 tGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatgat                                                                                                                                              >  2:432396/1‑109 (MQ=255)
                                                                                                                                                                                                  gCCGGTGTGAGTGGGTCGGTGCACTTGAGTTGCTGGTGAATGTTACTCGCGAATATTCTCTGGGCGGTGGCTTCGTACACGTGGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTGAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTAGTGGTATTTTGCCGATTGGCGTACTGGCACTGATGGCGATCAGCGGTGCGTTAAATGGCt                     <  2:161879/229‑1 (MQ=255)
                                                                                                                                                                                                  gCCGCTGTGAGTGAGTCGGGGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCt                     <  1:641642/229‑1 (MQ=255)
                                                                                                                                                                                                        gtgggtttgtCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCt               <  2:653502/221‑1 (MQ=255)
                                                                                                                                                                                                         tgggtgattCGGTGTCATTGAGTTGCTGGTTAATGTTCTGCGCCAATATTCTCTGGGCGGTTGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAAGACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCt               <  1:466459/225‑1 (MQ=255)
                                                                                                                                                                                                         tgggtgaggCGGGGCCATTGGGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCt               <  1:584082/225‑1 (MQ=255)
                                                                                                                                                                                                         tgagtgggTCGGTGCCATTGATTTGCTGGTTAATGTTCCTCGCCAATATTCCCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCt               <  1:599322/228‑1 (MQ=255)
                                                                                                                                                                                                         tgagtgagTCGGTGCCATTGAGTTGCTGGTTAATGTTGCGCGCCAATATTCTGTTGGCGTTGGCTTACGACACGCAGTAGGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCt               <  1:213748/228‑1 (MQ=255)
                                                                                                                                                                                                         tgagtgagTCGGTGCCATTGAGTTGCAGGTTAATGTTCCTCGCCAATATTCCCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCt               <  1:498913/228‑1 (MQ=255)
                                                                                                                                                                                                                  cGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTa     <  1:167403/229‑1 (MQ=255)
                                                                                                                                                                                                                   ggTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTAc    >  1:488640/1‑229 (MQ=255)
                                                                                                                                                                                                                    gTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGCTTATTGGTATTTTGCAGATTTGCGTACTGGCACTGATGGCGATCATCGGTGATGTAAATGGCTTAGGGTGGGGatttgacg   >  2:703104/1‑223 (MQ=255)
                                                                                                                                                                                                                     tGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGCTTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTg  >  1:724307/1‑229 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                            
AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGTGAAGATTGGCATTAAATCCACGGCAATCCTGTTCGGCCAATACGATAAATTGATTATTGGTATTTTGCAGATTGGCGTACTGGCACTGATGGCGATCATCGGTGAGTTAAATGGCTTAGGCTGGGGATATTACTG  >  NC_000913/4253297‑4253737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: