Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	644,420	A→C	17.6%	V195G (GTG→GGG)	rna ←	ribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	644,420	0	A	C	17.6%	115.2 / 2.0	62	V195G (GTG→GGG)	rna	ribonuclease I
Reads supporting (aligned to +/- strand):  ref base A (16/33);  new base C (10/3);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 9.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGATTAATAACCCGCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTCACTTAGTTTAGCGGCCGTTTCCAGTGATAATCCGGTTTCCGGCG  >  NC_000913/644192‑644639                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             gtgttttAATACCCCCTTTTAACAATAAAAAAGGTTTGGCCACAGTTCCCTGGGGGAGGTTGGGGTAAGAATGATGTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑Ca                                                                                                                                                                                                                              <  2:724606/224‑1 (MQ=255)       taataaCCCGCTTTATCAATCCAAAAGGTTTTGCCACAGTTACCTGGGTGGGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGAGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGtt‑t                                                                                                                                                                                                                       <  2:262471/229‑1 (MQ=255)         ataaCCCGCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGGGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGCCGGCT‑CACTGtt‑tt                                                                                                                                                                                                                      >  1:582883/1‑228 (MQ=255)            aCCCGCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTTcc                                                                                                                                                                                                                   >  1:597726/1‑228 (MQ=255)                gCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGGCAAAGTCACGTCGGCC‑TCCTGtt‑ttttccgaaa                                                                                                                                                                                                              >  2:430974/1‑221 (MQ=255)                 cTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCCCTTTTCCCCAGCTTTTGGCAAAGCCGGCGTCAAGGTCACGGCGGCT‑ACCCGTT‑TTTCTCTAAtt                                                                                                                                                                                                             >  1:298918/1‑229 (MQ=255)                 cTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGAGGAGCGTTGATGGCGTCAGCTTTGATTGAGATCTGAATTTCAGTCAAATACGCATGGTTACCCTGGCACTTTAGCTTAACTGCTGTAAAGTTCGCTTTTCCCCAGCTTTTGGCAAAGTCTGCGCCAAAGTCACGGCGGTT‑CAATACT‑TTTCCGGAAtt                                                                                                                                                                                                             >  2:237887/1‑229 (MQ=255)                   ttAACAATCAACAAGGTTTTGCCAAAGTTACCTGGGTGGGGTTGTGGCAAGAATGAGGTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTAt                                                                                                                                                                                                           <  2:597725/229‑1 (MQ=255)                       caatccCAAAGGTTTTGCCCCAGTTACCGGGGGGAGGTTGGGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGc                                                                                                                                                                                                       <  2:118147/229‑1 (MQ=255)                         atcaCAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTTATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTGGGCAAAGGCGGGGTCACAGTCACGGCGGCT‑CCTTTTT‑TTTCCGCAATTTTGCGCaa                                                                                                                                                                                                     >  1:291058/1‑229 (MQ=255)                              aaaGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCCGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGTT‑CCCCGTC‑TTTCTGTAATATTCCGCCAGGaaa                                                                                                                                                                                                >  2:170051/1‑228 (MQ=255)                                  gTTTTGCCACAGTTCCCTGGGTGAGGGTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTc                                                                                                                                                                                            <  1:468183/229‑1 (MQ=255)                                    tttGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGGT‑CACCGTT‑TTACCGCAATTATTCGCAAGACATTTTcc                                                                                                                                                                                           >  1:124156/1‑228 (MQ=255)                                     ttGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CCCCGTT‑TTTCCGTAATTTTCCGCAAGGAATTTTcc                                                                                                                                                                                           >  1:275760/1‑227 (MQ=255)                                     ttGCCACAGTTACCTGGGTGAGGATGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGGT‑CACTGGT‑TTTCCGTCATTATCCGCAAGGAATTTTcc                                                                                                                                                                                           >  1:712718/1‑227 (MQ=255)                                      tGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAATGGCGGCGTCAAAGTCACGGCGGCTCCCCTGTT‑TTACGGCATTTATCGGC‑AGGGATTTTACGGc                                                                                                                                                                                        >  2:670224/1‑229 (MQ=255)                                       gCCAGAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGAGCGAGATCTGAATTGCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCt                                                                                                                                                                                       <  1:170051/229‑1 (MQ=255)                                        ccACAGCTACCTCGGCGTGGATGTGGCCAGCATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATAGAGATCTGAATTTCAGTCAAATACGCAGGGGTACCCTGGCACGTTAGTTTAACTCCTTTCACGTTCCTTTTTCCCCAGCTTTTGGCAGAGGCGGCGTCCAAGCCAGGGCGGCT‑TACCGTT‑TTACCGTAACAATCCGCAAGGGACTTTcgcgccg                                                                                                                                                                                      >  2:12710/1‑223 (MQ=255)                                          acaGTTACCTGGGTGAGGTTGTGGGAAGAATGAGGTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCg                                                                                                                                                                                    <  1:70578/229‑1 (MQ=255)                                            aGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CCCTGTT‑TTTCCGGAATTATCCCCAAGGAATTTTCCGGCCTCGct                                                                                                                                                                                  >  1:522778/1‑229 (MQ=255)                                                         aGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCt                                                                                                                                                                     <  1:231337/229‑1 (MQ=255)                                                              gtgGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGAtt                                                                                                                                                                <  1:313038/229‑1 (MQ=255)                                                                    agagTGAGTTTTCAAAAAGGGGAGCGGTGAAGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATCCGCAGGGTTACCCTGGCAGGTTAGCCAAACTGCTTTCACGTTCTCTGTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCg                                                                                                                                                          <  2:360185/229‑1 (MQ=255)                                                                    aGAATGAGTTTGGAAGAAGCGGAGCGTTGAGGGCGTCAGCTTTGATCGAGATCTGAATTTCAGGCAAATTCGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCg                                                                                                                                                          <  1:248198/229‑1 (MQ=255)                                                                     gAATGGGTTTGCAGAAAGGGGAGGGTTGATGGCGTCGGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCg                                                                                                                                                          <  2:47346/228‑1 (MQ=255)                                                                     gAATGAGTTTGCAGCAAGCGGAGCGTTGGTGGGGTCAACTGTGATCGAAACCTGAATTTCAGTCAAATCCGCAGGGTTGCCCGTGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCCTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCg                                                                                                                                                          <  1:237887/228‑1 (MQ=255)                                                                      aaTGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTa                                                                                                                                                        >  1:272359/1‑229 (MQ=255)                                                                       aTGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTa                                                                                                                                                        <  1:430974/228‑1 (MQ=255)                                                                       aTGAGTTTGCAAAAAGTGGAGCGTTGTGGGCGTCAGCTTTGATCGAGATCTTAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTGTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTa                                                                                                                                                        <  2:140294/228‑1 (MQ=255)                                                                         ggggttGAAGAAAGGGGAGGGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATTCGCAGGGTTCCCCTGGCATGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCa                                                                                                                                                     <  2:582882/225‑1 (MQ=255)                                                                         gggTTTGCAGAAAGCGGAGCGTTGATGGGGTCAGCTTTGACCGAGATCTGAATTTCAGTCAAATCCGCAGGGTTCACCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCa                                                                                                                                                     <  1:284608/227‑1 (MQ=255)                                                                          aGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGCAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTTAGTGGTAccct                                                                                                                                                    >  1:676037/1‑227 (MQ=255)                                                                               tcaaaaaGCGGGGGGTTGGTGGCGTCAGCTTTGAACGGTATTTGAATTTCCGTCAAATACGACGGGTTACCCTGGCCAGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCAGCGTAc                                                                                                                                               <  2:298918/225‑1 (MQ=255)                                                                                    aaGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑CTACCGTCATTATCCGCCAGGAATTTTCCGGCTTCCCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGGAcccgag                                                                                                                                          >  1:216379/1‑225 (MQ=255)                                                                                       cGGAGCGTTGGTGGCGTCAGCTTTTGTCGTGATTTGAATTTCAGTCAAATACTCAGGGTTACCCTGGAACGTTAGCGTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTAt                                                                                                                                       <  2:243500/229‑1 (MQ=255)                                                                                       cGGAGCGTTGATGGCTGCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTAt                                                                                                                                       <  1:428507/229‑1 (MQ=255)                                                                                              ttGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCGAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTc                                                                                                                                  <  1:168924/227‑1 (MQ=255)                                                                                                  tggggtccacTTTTACCGAGATCTGAATTTCAGTCAAATCCGCAGGGTTCCCCTGGGACGTTAGGTTAACTGGTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGAt                                                                                                                             <  2:272359/219‑1 (MQ=255)                                                                                                  tGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGAt                                                                                                                             <  1:77930/228‑1 (MQ=255)                                                                                                           cTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCC‑CCCTGTT‑TTTCCCTAATTATCCGCCCGGGATTTTCCGCCTTCCCTCTCTTTAATTTCTTTATTCATCCCTACCATCGTACCGACGAATCCGTCCCGATCAAAACCaaa                                                                                                                   >  2:335217/1‑229 (MQ=255)                                                                                                             ttGAACGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTGAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGc                                                                                                                 <  1:743091/229‑1 (MQ=255)                                                                                                                  tgggaTCTGAATTTCCGTCAATTTCGCACGGTTACCCTGGCACGTTAGCTTAACTCTTTTCCCGTTCTCTTTTCCCCATCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAgggt                                                                                                            <  2:87272/226‑3 (MQ=255)                                                                                                                  cGATATCTGAAGTTGAGTCAATTTCGTAGGGTTCCCCTGTGCCGGTAGCTTAACTTCTTTCACGTTCTTGTTTTCCCAGCTTTTGGCAAAGGCTGCGTCAAAGTCACGGCGGCT‑CCCTGTT‑TTACCGTAATTAGCCGCATGGAATTTTCCGGCTTCGCTCACTTTAATGTCTTGATTCAGGCGTTCCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCa                                                                                                            <  2:64907/229‑1 (MQ=255)                                                                                                                  cGAGATCTGAATTTCAGTCAAATACGCAGGGTTAACCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCa                                                                                                            <  1:256943/229‑1 (MQ=255)                                                                                                                     gaTCTGAATTTCAGTCAAATACGCAGGGTTACCCTGTCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATTCGCCAAGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCATGAGTGACATCGTAACACCGCTACAGTCCGGATCAAAACCCAAACCGGCAcca                                                                                                         >  1:444885/1‑228 (MQ=255)                                                                                                                     gaTCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCGCCAGCTTTTGGCAAAGGCGGCGGCAAAGTCACGGCGGTT‑GCCTTTT‑TTACCGTAATTATCCGCCAGGGATTTTCCGCGTACCGCCTCTTTAATTTTTTGTTTCACTCGTACCACCGTACCGAAGGATTCCTCCGGTTCCAAACCCAAACAgagccag                                                                                                         >  2:537848/1‑223 (MQ=255)                                                                                                                           aaTTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCCAGGCATTTTCCGGCTTCGCACTCTTTAATTTTTTGTTTCAGGCGTAACATCGTACCCAAGTATGCGTCCGGTTCAAAACCAAACACGGCACAGGGTTTg                                                                                                   >  2:172822/1‑229 (MQ=255)                                                                                                                                   tCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CCCTGTT‑TTACCGTAATTATCCGCCAGGAATTTTTCGGCTTCGCTCTCTTTAATTTCTTGATTCCGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAACCAGGCACCCGGTTTGGCATCttt                                                                                           >  1:45318/1‑228 (MQ=255)                                                                                                                                      aagtcgcgGGTTTTCCTTGGCAGGTTGGGTTACCTGCTTTCACGTGCTCTTTTCCCCATCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGt                                                                                         <  2:687150/224‑1 (MQ=255)                                                                                                                                       aTACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTa                                                                                        <  1:172822/228‑1 (MQ=255)                                                                                                                                             aGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTTCCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTTATTTAGCCGTACCATCGTACCCAAGAATTCTCCCGGATTCAAACCAAAACAGGCCCCCGGTTTGGCCTTTTCTTAGGCTTcc                                                                                 >  2:6419/1‑229 (MQ=255)                                                                                                                                               ggTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGGTTCGCTCTCTTTAATTTCTTGATTCAAGTGTACCATCGTACCGAAGTATGCGTCCGGGTCAAAACCAAAGAAGTGACCGTGTTTGGCATATTCGTAGCGTTCca                                                                                >  1:114727/1‑228 (MQ=255)                                                                                                                                                               tAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAAGCGTACCATCGTACCGAAGTATGCGTCCGGGTCAAAACCAAAGCAGGCACCGTGTTTGGCAATTTCGTAGCGTTCCAGGCAGGAAGGTCCGCCa                                                               >  1:258429/1‑229 (MQ=255)                                                                                                                                                                  cTTAACTGTTTTCCCGTTCTCTTTGCCCCCGTTTTTGGACAAGGCGCCGTCAAAGTCACGGCGGCT‑CACGGTT‑TTACCGTAATTATCCTCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCt                                                            <  2:114727/229‑1 (MQ=255)                                                                                                                                                                  cTTAACTGCTTTCACGTTCCCTTTTCCCCAGCTTTTGGCAGAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTGCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCt                                                            <  1:12710/229‑1 (MQ=255)                                                                                                                                                                        tGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGGT‑TTTCCGTAATTATCCGCCAGGAATTTTCCGGGTTCGCTCTCTTTAATTTCTTGATTCATGCGTACCATCGTACCGAAGTATGTGTCCGGATCAACACCAAAGCACGCACCGTGCTTGGCATATTCGTAGCGTTACAAGCAGGAACGTCCGCCAGCTCCTg                                                        >  1:447456/1‑227 (MQ=255)                                                                                                                                                                                cGTTCTCTTTTCCCCAGCTTTTGGGAAAGGCGGCGTCAAAGCCCCGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCTAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCCTCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTc                                              <  2:447456/229‑1 (MQ=255)                                                                                                                                                                                    tttttttCCCCAGCTTTTGGCAATGGCCGCGTCAAAGTCACGGCGG‑T‑CCATGTTGTTCCCGTAATTAGCCGCAAGGAATTTTGCGGCTTCGCTCTCTGTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGGGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTCACtt                                          <  2:13659/226‑1 (MQ=255)                                                                                                                                                                                    ctctTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCCAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGCCGTACCATCCTACCGAAGTATGCGTCCGGGTCACAACCCAAGCAGGCACCGTGTTTGGGATATTCGTATCGTTCCAGGCAGGAACGCCCCCCACCCCCCGCCTTGACTTCAcct                                          >  2:515531/1‑227 (MQ=255)                                                                                                                                                                                                        cAAAGGCGGCGTCAAAGTCACGGCGGCT‑TCATGTT‑TTACCGTAATTAGCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTCACTTAGTTTAGCGGCCGTTTCCAg                      <  1:77886/229‑1 (MQ=255)                                                                                                                                                                                                                          gccggcggCT‑CACTGGT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTGCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTCACTTAGTTTAGCGGCCGTTTCCAGTGATAATCCGGTTTCCg     <  1:503489/226‑1 (MQ=255)                                                                                                                                                                                                                          ctcggcggCT‑TACTTTT‑TTACCGTAATTACCCGGAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTCACTTAGTTTAGCGGCCGTTTCCAGTGATAATCCGGTTTCCg     <  1:526046/226‑1 (MQ=255)                                                                                                                                                                                                                            cggcggCT‑CACTTTT‑TTACAGTAATTAGCCGCAAGGAATTTTCCGGTTTCGCTCTCTTTAATTGCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTGTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTCACTTAGTTTAGCGGCCGTTTCCAGTGATAATCCGGTTTCCGGCg  <  2:275760/229‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             GATGATTAATAACCCGCTTTATCAATCACAAAGGTTTTGCCACAGTTACCTGGGTGAGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGCGTTGATGGCGTCAGCTTTGATCGAGATCTGAATTTCAGTCAAATACGCAGGGTTACCCTGGCACGTTAGCTTAACTGCTTTCACGTTCTCTTTTCCCCAGCTTTTGGCAAAGGCGGCGTCAAAGTCACGGCGGCT‑CACTGTT‑TTACCGTAATTATCCGCAAGGAATTTTCCGGCTTCGCTCTCTTTAATTTCTTGATTCAGGCGTACCATCGTACCGAAGTATGCGTCCGGATCAAAACCAAAGCAGGCACCGTGTTTGGCATATTCGTAGCGTTCCAGGCAGGAACGTCCGCCAGCTCCTGGCATGACTTCACTTAGTTTAGCGGCCGTTTCCAGTGATAATCCGGTTTCCGGCG  >  NC_000913/644192‑644639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑