breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,316,565 C→T 32.0% T130I (ACC→ATC)  rcsB → response regulator in two‑component regulatory system with RcsC and YojN
RA 2,976,996 C→A 9.4% S133* (TCA→TAA)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
RA 2,977,092 G→A 10.9% W165* (TGG→TAG)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
JC 3,523,578 +CAA 5.6% coding (708/2553 nt) mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
JC JC 3,526,449 IS5 (+) +4 bp 46.1% intergenic (‑300/‑17) nudE ← / → yrfF adenosine nucleotide hydrolase; Ap3A/Ap2A/ADP‑ribose/NADH hydrolase/putative RcsCDB‑response attenuator, inner membrane protein
RA 3,527,606 Δ1 bp 30.6% coding (1138/2136 nt) yrfF → putative RcsCDB‑response attenuator, inner membrane protein
RA 3,528,603 Δ1 bp 11.3% coding (2135/2136 nt) yrfF → putative RcsCDB‑response attenuator, inner membrane protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,992,438 T→G 20.8% L429* (TTA→TGA)  cyaA → adenylate cyclase
JC 3,992,469 Δ4 bp 8.3% coding (1317‑1320/2547 nt) cyaA → adenylate cyclase
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,190 A→G 21.2% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 23.4% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 18.7% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 10.2% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 6.2% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,326,088 T→G 7.5% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,147 T→C 6.5% intergenic (‑406/+252) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2533672 2536468 2797 81 [79] [79] 82 ptsH–[pdxK] ptsH,ptsI,crr,[pdxK]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =66 (0.410)76 (0.500) 60/486 NT 55.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 59 (0.390)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780561 (0.380)92 (0.610) 66/486 NT 61.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 59 (0.390)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1979270NA (NA)10 (0.060) 8/516 NT 5.6% noncoding (1/768 nt) IS1 repeat region
?NC_000913 3527247 = 169 (1.050)coding (779/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
* ? NC_000913 = 2024339174 (1.080)12 (0.070) 12/518 NT 6.5% coding (372/624 nt) rcsA transcriptional regulator of colanic acid capsular biosynthesis
?NC_000913 2025115 = 172 (1.070)coding (130/228 nt) yodD uncharacterized protein
* ? NC_000913 3845531 =126 (0.780)77 (0.500) 51/498 NT 36.0% coding (1568/1767 nt) adeD cryptic adenine deaminase
?NC_000913 = 3992760 153 (0.990)coding (1608/2547 nt) cyaA adenylate cyclase
* ? NC_000913 4295832 =NA (NA)6 (0.040) 3/440 NT NA intergenic (+38/+604) gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
?NC_000913 = 4296394 NA (NA)noncoding (560/600 nt) RIP321 (repetitive extragenic palindromic) element; contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element; contains 11 REP sequences and 4 IHF sites