BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	67,887:2	+T	66.4%	coding (451/1503 nt)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,887	2	.	T	66.4%	294.9 / 139.8	116	coding (451/1503 nt)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base . (19/20); new base T (40/36); total (59/57)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

Only 100 of 116 total aligned reads displayed.

TGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTG  >  NC_000913/67609‑68173

tgtgGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGTCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATTGCCAAATCGGCAGACTTTCAGATGACGGGTCTCCTGTTTAGAGACCGCCTGACGCATCTAGGAGCCGAACCGCTCATTGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTa                                                                                                                                                                                                                                                                                      >  2:812663/1‑289 (MQ=255)

   ggCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGGTCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGTGCTTGTTTATCCTGCCA‑‑G‑‑T‑gtgaccggta                                                                                                                                                                                                                                                                                    <  1:452553/288‑10 (MQ=255)

               ggtgacgccgcTTTCGTCCTCATCGACCATCGCGTTACCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAAAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGc                                                                                                                                                                                                                                                                       <  1:345513/280‑1 (MQ=255)

                   tAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCa                                                                                                                                                                                                                                                                             >  2:302580/1‑281 (MQ=255)

                   tAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCa                                                                                                                                                                                                                                                                             <  1:302580/281‑1 (MQ=255)

                      cagcTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGAc                                                                                                                                                                                                                                                                   >  1:513712/1‑288 (MQ=255)

                       agcTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGc                                                                                                                                                                                                                                                                 >  1:562230/1‑289 (MQ=255)

                         cTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCAt                                                                                                                                                                                                                                                               >  1:310221/1‑289 (MQ=255)

                           ttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCCCATGGGCTTGTTTATCCTGCCA‑‑GTTT‑GACCGGTAACCAAGGCATGTTGCTGACGCATAc                                                                                                                                                                                                                                                             >  1:460676/1‑289 (MQ=255)

                           ttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCG‑CCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATAc                                                                                                                                                                                                                                                             >  1:995689/1‑288 (MQ=255)

                           ttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTAACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGATTGTTTATCCTGCAAGTG‑‑T‑GACCGGTTACCACGGCATGTTCCTGACACATa                                                                                                                                                                                                                                                              >  1:442489/1‑288 (MQ=255)

                            tCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATAcg                                                                                                                                                                                                                                                            <  1:777347/289‑1 (MQ=255)

                            tCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATAcg                                                                                                                                                                                                                                                            <  1:784715/289‑1 (MQ=255)

                                            gcgcGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGCACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTCATCGCCAAATCGGCAGACTTTCAGCTGACGGGTATCCTGTTTAGCGACCGCCTGACGCATCCCGGAGCCGTTACGCCCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGAAACCCCGGCCTGTTTCTTCCCCATTcgcgtccccatgccggc                                                                                                                                                                                                                                             >  2:405958/1‑275 (MQ=255)

                                            gcgcGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGCCAGACTTTCAGATGACGGGTACCCTGTTTAGATACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCATGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTTGTTACGCATACGCTGGCCACTGAAGCCg                                                                                                                                                                                                                                            >  2:953811/1‑289 (MQ=255)

                                                   aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGGTCTGTGCGGAAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTTTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGa                                                                                                                                                                                                                                   <  1:812663/289‑1 (MQ=255)

                                                   aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACGTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGa                                                                                                                                                                                                                                   <  2:1014143/289‑1 (MQ=255)

                                                                         ttCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCCCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggt                                                                                                                                                                                                                >  1:349191/1‑288 (MQ=255)

                                                                          tCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATAGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGAGGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggtt                                                                                                                                                                                                               <  1:814570/288‑1 (MQ=255)

                                                                           caccacCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAACTCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCCGCCTCATCCGCGCGCCAATGAAGCCGAACTCGCCACCACCATGTGCCGTCGggttca                                                                                                                                                                                                             >  2:320730/1‑289 (MQ=255)

                                                                             ccaccTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttcagg                                                                                                                                                                                                           <  2:513712/289‑1 (MQ=255)

                                                                                 cTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GAACGGTAACCACGGCATGTTGCTGCCGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTTCAGTCTGGTTCCGGCTCAtc                                                                                                                                                                                                     >  2:583644/1‑288 (MQ=255)

                                                                                  tGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATaa                                                                                                                                                                                                    >  1:778254/1‑289 (MQ=255)

                                                                                   gCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCTCGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑GTTT‑GACCGGTAACCACGGCATGTTGTTGACGCATACGCTCGCCAATGCAGCCGAACTCGCGACCCCCATGTGCAGTCTGGTTCACGTTCATa                                                                                                                                                                                                     >  2:615892/1‑289 (MQ=255)

                                                                                      cccgATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACGTGATCTGGGCGGCAACTTTATCGCCAGCGGGGACCGCCACTTCACGCATGTGATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTAGCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTCCAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                <  2:638530/286‑1 (MQ=255)

                                                                                      ccAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                <  1:873780/289‑1 (MQ=255)

                                                                                        aaaTCGCGAACCGCCCAGGGATTGTCGTGGAAACCGAACTTTATCTGTGCGGCATCTTGATAGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGTTGACGGGTATCCTGTGTAGAGACCGCCTGACGCAGCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGTAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                <  2:442489/287‑1 (MQ=255)

                                                                                          aTCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCa                                                                                                                                                                                              >  1:706576/1‑289 (MQ=255)

                                                                                           tCGCCAACCGCCCAGGTTTTTACTGCGCAACCGAACTGGTTTTGTCCGGCAACTTTTTCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                <  2:394398/286‑1 (MQ=255)

                                                                                           tCGCCAACCGCCCAGGTATTGACGGAGAAACCCAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGCACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCa                                                                                                                                                                                              <  1:85984/288‑1 (MQ=255)

                                                                                             gCCAACCGCCCAGGTATTGACGGAGAAACCTAACTTGATCTGTGCGGCAAATTTAGCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCatat                                                                                                                                                                                           <  1:890709/289‑1 (MQ=255)

                                                                                                acccGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGGGCTGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATa                                                                                                                                                                                      <  1:583644/287‑1 (MQ=255)

                                                                                                aaCCGCCCAGGTATGTGCGGAGAAACCGAACTTGATCTGGTCGCCAACTTTATCGCCTTCGGTGCCCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATa                                                                                                                                                                                      <  2:639469/289‑1 (MQ=255)

                                                                                                 aCCGCCCGGTGATTGACGGAGAAACCGAACTTGGTCTGGGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGGAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAc                                                                                                                                                                                     <  2:909727/289‑1 (MQ=255)

                                                                                                 aCCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCCTACGCTCGCCCATGAAGCCGCACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGa                                                                                                                                                                                        >  1:635135/1‑288 (MQ=255)

                                                                                                 aCCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGAt                                                                                                                                                                                       >  1:42957/1‑287 (MQ=255)

                                                                                                    gCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGAt                                                                                                                                                                                       >  1:355493/1‑286 (MQ=255)

                                                                                                     cccAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCCACCGCCCTGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGAt                                                                                                                                                                                       >  2:515635/1‑285 (MQ=255)

                                                                                                      ccAGGGATGGACGGAGAAACCAAACTTGCTCTGTGCGGCAACTTTATCGCCCTCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAc                                                                                                                                                                                     <  1:284063/286‑1 (MQ=255)

                                                                                                         cgccgcagaagagaAACCGAACTGGTGCTGGTCGACAACTTTAGCGCCATCGGGGACCGCCACTTCACGCATGTTATCGCCAAAGCGGCAGACGTGCAGATGACGGGTATCCTGTTTAGGGACCGCCTGACGCATCCAGGAGCCGATACACTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATAGCGATACTGTccc                                                                                                                                                                               <  2:611901/279‑1 (MQ=255)

                                                                                                                acgtggaAACCGAACTTGATCTGGGGGGCAAGTTTATCGCCAGCGGTGACCGCAACTGCACGAGTGTTATCGACAACTGGGCAGACGTTCAGATGACGGTTATCTTGTTTAGAGACCGCCTGAGGCATCCAGGAGAAGGTACGCTAATGGGCTTGGTTATCCTGACAGGG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCa                                                                                                                                                                         <  2:635135/283‑1 (MQ=255)

                                                                                                                aCGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCCCGGCCTGTTGCTGACGCATACGAGCGCCAATGAAGACGCACCTGACACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCCTACCGATACTTTTCCACgg                                                                                                                                                                           >  2:284063/1‑286 (MQ=255)

                                                                                                                  ggAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgcg                                                                                                                                                                      <  1:822648/289‑1 (MQ=255)

                                                                                                                   gagaAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgcg                                                                                                                                                                      <  2:310221/288‑1 (MQ=255)

                                                                                                                    agaAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGcgccgc                                                                                                                                                                  >  1:664732/1‑289 (MQ=255)

                                                                                                                      aacccGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGcgccgc                                                                                                                                                                  <  1:212725/286‑1 (MQ=255)

                                                                                                                       aaCCGAACTTCTTCTGTGCGGCAACTTTGTCGCCATCGGTGACCGCCACTTCACGCATGTTAGCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCcgcg                                                                                                                                                                 <  2:778254/287‑1 (MQ=255)

                                                                                                                             acggactcgttgCGGCACCTTTTTGCCCATCGGTGACCGCCACTTTACGCATGTGATCGCCGAATCGGCATACTTTCAGAGGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCGTGGGCTTGTTTATCCGGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGaa                                                                                                                                                            <  2:282079/278‑1 (MQ=255)

                                                                                                                              cTTGATCTGTGGCGCAATTTTATCGCCATCGGGTACCGCCACTTCACGCATGTTATCGCCAAAGCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTGTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACt                                                                                                                                                          <  2:969833/289‑1 (MQ=255)

                                                                                                                                 gttCTGTGCGTCAACTTTATCGCCACGGGTGACCGCCACTTCCCACATGTTATCTCCGAATCGGCAGACTTTCAGATGACTGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGgtg                                                                                                                                                     <  2:693118/287‑1 (MQ=255)

                                                                                                                                       tgCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTAAACTggg                                                                                                                                                       <  1:12448/283‑1 (MQ=255)

                                                                                                                                       tgCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGCAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTTAACTGGGTGTGGAAt                                                                                                                                               >  1:693118/1‑289 (MQ=255)

                                                                                                                                       tgCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTACATATCGGTACTGTCCCACGGAAGCGCCGCGTTGAACTGGGTGTGGAAt                                                                                                                                               >  1:246900/1‑289 (MQ=255)

                                                                                                                                          ggCACCTTTATCGCAATCGTTGACCGCCACTTCACGCATGTTCTCGCCAAATCGGCAGACTTTCAGAGGACGGGTATCCTGTTTAGAGACCGCCGGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAAt                                                                                                                                               <  2:995689/288‑1 (MQ=255)

                                                                                                                                            cAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgca                                                                                                                                           >  1:242066/1‑288 (MQ=255)

                                                                                                                                                 ttATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCCGGTTCAGGTTCATAAGGTCCATCTCGATACTGTCCCACCGCAGCGCCGCGTTGAACCGGGTGTGGAAGTg                                                                                                                                             >  2:12448/1‑283 (MQ=255)

                                                                                                                                                         aTCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTttgt                                                                                                                               >  2:1097612/1‑289 (MQ=255)

                                                                                                                                                              tGACCGCAAATTAACGCGTGTTAACCCCCACTCGGGAACCTTTCAGTTGCCGGGTATCCTTTTTAGAAACCGCCGGACGGATCCAGAGGCCGATCCGCTCATGTGCTTGTTGATCCTGCCAGTG‑‑T‑GACCGTTAACCACGGCATGTTGCTGACGCATACGCGCGCCAGTGAAGCCGAACGCGCGACCTCCGTGTGCAGGCCGGTTCAGGTTCAAAAAGTCCTGATCGATACTGTCCCGCGGCAGCTCCGCGTTGAACTGGGTGTGGCATTGCAGCAACGTTCTGTTGAg                                                                                                                           <  2:784236/288‑1 (MQ=255)

                                                                                                                                                                   gaatagtggacagtGTTAGCGCCAAATCGGCAGACTTTCAGATGACGGTGATCCTGTTTTGAGACCGCCTGACGCATCCAGGGGCCGTTACGGTAATGGGCTTGTTAATCCTGCCA‑‑G‑‑T‑GACCGTTAACCCCGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCa                                                                                                                   <  2:568238/276‑1 (MQ=255)

                                                                                                                                                                     cACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCa                                                                                                                   <  2:355493/289‑1 (MQ=255)

                                                                                                                                                                          cACGCAGTTTATCGCCAAATCGGCAGACTTTCAGATGGCGGGTATCCTTTTTAGAGACCGCCTCACGCATCCAGGGACCGATACGCTCATTGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCg                                                                                                              <  1:940908/289‑1 (MQ=255)

                                                                                                                                                                            cGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGtt                                                                                                            >  1:607576/1‑289 (MQ=255)

                                                                                                                                                                                  ttATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                >  2:179471/1‑199 (MQ=255)

                                                                                                                                                                                  ttATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                <  1:179471/199‑1 (MQ=255)

                                                                                                                                                                                      cGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAtt                                                                                                  >  1:307714/1‑289 (MQ=255)

                                                                                                                                                                                       gCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAc                                                                                                                                                                                     >  2:202293/1‑205 (MQ=255)

                                                                                                                                                                                          aaaTCGGCAGCCTTTCAGATGGCGGGTTTCCTGTTTAGGGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTgg                                                                                              <  2:656882/289‑1 (MQ=255)

                                                                                                                                                                                           aaTCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTgg                                                                                              >  1:656882/1‑288 (MQ=255)

                                                                                                                                                                                           aaTCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGc                                                                                             <  1:1097612/289‑1 (MQ=255)

                                                                                                                                                                                           aaTCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGGGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGAt                                                                                                         >  1:568238/1‑275 (MQ=255)

                                                                                                                                                                                                 cAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCCCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCACGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGGGGTATTGCAGCAAAGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGga                                                                                       >  2:850828/1‑289 (MQ=255)

                                                                                                                                                                                                      tttCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGACGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTACCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAgg                                                                                  >  2:699268/1‑289 (MQ=255)

                                                                                                                                                                                                               aCGGGTATCCTGTTTAGAGACCGCCTGACGCATCTAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATAAGCCCGCCAATGAAGCCGAACTCGCCACCGTCATTTGCAGTCTGGTCCCGGTTCATAAAGCCCATATCGATACTGTTCCACGTCAGCTACGCGTTGAAATCGGTGTGGAATTGCAGCAACGGTTTGTTGATCATGGTCAGGTCGTTGATCACAATTTTGGCCGGGGAGAAGGTGTGCAgac                                                                         >  2:616715/1‑287 (MQ=255)

                                                                                                                                                                                                                cGGGTATCCTTTTTAGAGACCCCCTGACGCAACAAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGAAGTCTGGTTCGGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCcac                                                                       <  1:610817/288‑1 (MQ=255)

                                                                                                                                                                                                                cGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCCTACGCGCGCCAATGCAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGGAGGTGTGCAGcc                                                                         >  1:47906/1‑288 (MQ=255)

                                                                                                                                                                                                                cGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCAtgtg                                                                                                                                                                                                                         <  2:294169/142‑1 (MQ=255)

                                                                                                                                                                                                                      tCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCAcaccac                                                                  <  1:63220/289‑1 (MQ=255)

                                                                                                                                                                                                                           tttAGAGCCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                                                             <  1:1073237/289‑1 (MQ=255)

                                                                                                                                                                                                                            tgagtgaCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTGATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCaccagac                                                             <  2:349191/283‑1 (MQ=255)

                                                                                                                                                                                                                                agaCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCCCACCACCAGACCAgc                                                         >  1:136140/1‑288 (MQ=255)

                                                                                                                                                                                                                                     gCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGc                                                                                             >  2:854344/1‑247 (MQ=255)

                                                                                                                                                                                                                                     gCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACg                                                   >  1:110977/1‑289 (MQ=255)

                                                                                                                                                                                                                                     gCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                <  2:923712/146‑1 (MQ=255)

                                                                                                                                                                                                                                     gCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTc                                                                                                                                                                                                >  1:923712/1‑146 (MQ=255)

                                                                                                                                                                                                                                     gCCTGACGCATCCAGGAGCCGATACGCTCATGGCCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTGTCTGCCGCATACGCGCGCCAATGAAGCCGAACTAGCGACCGCCATGTGCAGTCTGGTTCAGGTGCATAAAGTCCATATCGATACTGACCCCCGGCAGCGCCGCGTTGAACTGCGTGTGGAATTGCAGCAACGGTTTGTTGAGCACGTTCTGGCAGGTGGTCAATATTTTGGCCGGGGAGAAGGAGTTCAGCGAGACCACCAGATCAGCCCAACg                                                   >  2:643670/1‑289 (MQ=255)

                                                                                                                                                                                                                                       cTGACGAATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCGGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATAGCGATACTGTCCCACGGCAGCGCCGCGTTGAACGGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGa                                                  <  1:681156/288‑1 (MQ=255)

                                                                                                                                                                                                                                                    gAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATtcg                                     >  2:67765/1‑288 (MQ=255)

                                                                                                                                                                                                                                                        cGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGt                                                                                                                     <  1:209655/204‑1 (MQ=255)

                                                                                                                                                                                                                                                        cGATACGCTCATGGGCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGt                                                                                                                     >  2:209655/1‑204 (MQ=255)

                                                                                                                                                                                                                                                                    gggctgtgttATCCTGCCG‑‑GA‑TGGACCGGGAACCACGGCATGTGGCTGACGCATAAGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCg                    <  2:706576/281‑1 (MQ=255)

                                                                                                                                                                                                                                                                     ggCTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCgg                   >  2:706375/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                       cTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCaaa                         >  2:233244/1‑281 (MQ=255)

                                                                                                                                                                                                                                                                       cTTGTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCaaa                         <  1:233244/281‑1 (MQ=255)

                                                                                                                                                                                                                                                                          gTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCCTAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCGGTCACGGATTGTTGAGCATGGTCAGGCCGTTGATCCCCACTTTGGCCGGGGAGAAGGTGTGCAGCCACACAACCAGACTAGCCCAAGGATCGTCATATCTCGGGTCGCGGCACATAACGGTTAtt              >  2:157739/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                          gTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGGCACCCCACCAGACCAGCGCAACGATTGTCGTAATTCGGGTCGCGGCCAATAGCGGGGAtt              >  2:917922/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                          gTTTATCCTGCCAGTG‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGAtt              >  1:1038640/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                          gTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTc            >  1:74185/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                            ttATCCTGCCAGTG‑‑T‑GGCCGGGAACCACGGGAGGTTGCTGACGCATACGCGCGCCAAAGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGATTCAGGTTCATAAAGTCCATATCGATACTGGCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCGGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTc            <  2:47906/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                                    ccA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCgtg  >  2:754387/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                                    ccA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCAACCCACCAGACCAGCGCAACGATCGGCGTAATTCGCGTAGGGGCAAATAGCGGTGACTTCATCCGGCctg  >  2:335918/1‑289 (MQ=255)

TGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCA‑‑G‑‑T‑GACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTG  >  NC_000913/67609‑68173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`