Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,805,981 T→C 6.4% K363E (AAA→GAA)  waaG ← UDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,805,9810TC 6.4% 200.5 / 2.2 61K363E (AAA→GAA) waaGUDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base T (25/32);  new base C (2/2);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

GATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATGCAGTAATAAATCAGCGGCT  >  NC_000913/3805704‑3806261
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                         
gATGAATATCGTGCCACTCGCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGc                                                                                                                                                                                                                                                                               <  2:790037/289‑1 (MQ=255)
    aaTAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTCTTCTGGCAAGCtata                                                                                                                                                                                                                                                                           >  2:848408/1‑289 (MQ=255)
     aTAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCACAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATAc                                                                                                                                                                                                                                                                          <  1:596311/289‑1 (MQ=255)
        gCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTCTTCTGGCAAGCTATACaaa                                                                                                                                                                                                                                                                       >  2:1006478/1‑289 (MQ=255)
          gTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAAt                                                                                                                                                                                                                                                                      >  2:503134/1‑288 (MQ=255)
            gCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATcc                                                                                                                                                                                                                                                                    <  2:851049/288‑1 (MQ=255)
                  ctctGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTAt                                                                                                                                                                                                                                                             <  1:503134/289‑1 (MQ=255)
                  ctctATCTTCGCCCAAAACGGGCTGCCGCAATGTGAGTAGATTTTTTATAATCTCTTTTAATGTCGACCCCTTGAGCCATTTAAGAAAATAGCGTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTAt                                                                                                                                                                                                                                                             <  2:189667/289‑1 (MQ=255)
                             cccAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATgg                                                                                                                                                                                                                                                  <  1:137249/289‑1 (MQ=255)
                                                                          tttAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAAc                                                                                                                                                                                                     >  1:99944/1‑289 (MQ=255)
                                                                             gaagtcaaccccTTGTGCCAATTTAGGAAAGTGCTTTTCCCGGGCAGTTCAAGGCGTAAAGTTCGGCGGGGCTTCAGTTCACGAAAGGCCTCGGCGGTTAGTTTTTTGACCTTCGGAAAAGCAACTTTACCACGCCCAAGTGGGGGCAGCGGCTCTTTTAAATCAACCCACTAAACCACCTGTTATGATAGCCGGGGGTTTTTTTGGGCAAGTGTACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCt                                                                                                                                                                                                  <  2:194756/281‑1 (MQ=255)
                                                                                 tcgtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTAc                                                                                                                                                                                              >  1:294438/1‑289 (MQ=255)
                                                                                  cgtcCCCTAATGCCATTTAAGAAAATAGCTTTTCCCGAGCAGTGCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCACTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTGTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACg                                                                                                                                                                                             <  2:693033/289‑1 (MQ=255)
                                                                                   gtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTg                                                                                                                                                                                                                                                                                     >  2:136384/1‑200 (MQ=255)
                                                                                   gtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTg                                                                                                                                                                                                                                                                                     <  1:136384/200‑1 (MQ=255)
                                                                                   gtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGt                                                                                                                                                                                            >  1:40495/1‑289 (MQ=255)
                                                                                   gtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGt                                                                                                                                                                                            >  1:769558/1‑289 (MQ=255)
                                                                                   gtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAAAGCTTTACGt                                                                                                                                                                                            >  1:365790/1‑289 (MQ=255)
                                                                                     ccccTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTaa                                                                                                                                                                                          <  1:938049/289‑1 (MQ=255)
                                                                                       ccTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTaaaa                                                                                                                                                                                        <  2:1076424/289‑1 (MQ=255)
                                                                                           gtgCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTc                                                                                                                                                                                    >  1:790478/1‑289 (MQ=255)
                                                                                                      gAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGt                                                                                                                                                                          >  2:1094626/1‑288 (MQ=255)
                                                                                                                tttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGaa                                                                                                                                                               <  2:40495/289‑1 (MQ=255)
                                                                                                                tttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGaa                                                                                                                                                               <  1:104180/289‑1 (MQ=255)
                                                                                                                tttCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGaa                                                                                                                                                               <  1:442161/289‑1 (MQ=255)
                                                                                                                            ttCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTAt                                                                                                                                                                                                                                                             >  2:670791/1‑183 (MQ=255)
                                                                                                                            ttCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTAt                                                                                                                                                                                                                                                             <  1:670791/183‑1 (MQ=255)
                                                                                                                            ttCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATg                                                                                                                                                                                                                   <  2:662115/225‑1 (MQ=255)
                                                                                                                            ttCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATg                                                                                                                                                                                                                   >  1:662115/1‑225 (MQ=255)
                                                                                                                            ttCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGAt                                                                                                                                                   >  2:35823/1‑289 (MQ=255)
                                                                                                                                     tAATGTACGAAGAGTCTCCAGTTCACCAAAGACCTCGCCGTTCAGTTTTTTGATCTCTGCAAAAGCATCTTTACCACGCCAACGTGTGGCAAGCGGCTCTTTGAATTCAAACATCTAAACCTCCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATTGAGGGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTTTTCCTGAGAGAAAGGTTCAGCGATGACCGTTc                                                                                                                                           <  2:311908/288‑1 (MQ=255)
                                                                                                                                      aaTGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTCTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCca                                                                                                                                         <  1:1006478/289‑1 (MQ=255)
                                                                                                                                               cgaGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGc                                                                                                                                 <  2:863101/288‑1 (MQ=255)
                                                                                                                                               cgaGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGc                                                                                                                                 <  1:38932/288‑1 (MQ=255)
                                                                                                                                                 aGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTTCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGtt                                                                                                                                                          <  2:672301/261‑1 (MQ=255)
                                                                                                                                                 aGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGtt                                                                                                                                                          >  1:672301/1‑261 (MQ=255)
                                                                                                                                                 aGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCAt                                                                                                                               <  2:294438/288‑1 (MQ=255)
                                                                                                                                                  gTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATc                                                                                                                              >  2:981386/1‑288 (MQ=255)
                                                                                                                                                               gAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATgcg                                                                                                                <  1:981386/289‑1 (MQ=255)
                                                                                                                                                                    aCCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTa                                                                                                            >  2:350522/1‑288 (MQ=255)
                                                                                                                                                                                   tttttGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTCTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCt                                                                                               <  1:848408/286‑1 (MQ=255)
                                                                                                                                                                                          ccTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGcgcg                                                                                                              <  2:919186/264‑1 (MQ=255)
                                                                                                                                                                                          ccTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGcgcg                                                                                                              >  1:919186/1‑264 (MQ=255)
                                                                                                                                                                                          ccTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTaaa                                                                                      <  1:35823/288‑1 (MQ=255)
                                                                                                                                                                                                   aaGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAAcc                                                                            <  2:99944/289‑1 (MQ=255)
                                                                                                                                                                                                        tCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGCGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCCTCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGTTAACCaagca                                                                       >  2:838780/1‑289 (MQ=255)
                                                                                                                                                                                                             aCCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGa                                                                   >  1:119348/1‑288 (MQ=255)
                                                                                                                                                                                                                aCGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGATCATTCTCCGCCCAGGCCATTCGCAATGGCGCCTGAGTTAAAGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCCATGACCGTTACACAATTTGAATCCGCAATATAATGCGCGTACCCCCATAACGCTGCTGTTAAAACAGGAAACCCAGCAGTGATCg                                                                >  2:212681/1‑288 (MQ=255)
                                                                                                                                                                                                                 cGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCCCCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGAGATCATTCTCCGCCAAGGCAATTCGCAATGGCGACTGAGTTAACTCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGCTGACCGTTCCACAATTGGCATCAACATTCTAATGCTCGTACCCAAATACCGCTGTTGTTAAAACAGGTAACCtagcgatgacccct                                                              >  2:785414/1‑279 (MQ=255)
                                                                                                                                                                                                                                             aTTCAACCATCTAAACCTCCTGTAAGTATATCCGCGGGTTTTTCTGGCAAGCTATACAAATCCTGGGTATCGGCATAATGGCGAGTATTCTCCGCCCAGGCCATTCGCAATGGCGACTTAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTTAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCt                                  <  1:726795/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      tCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAAcc                                                                            >  2:531004/1‑238 (MQ=255)
                                                                                                                                                                                                                                                      tCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAAcc                                                                            <  1:531004/238‑1 (MQ=255)
                                                                                                                                                                                                                                                           accaccTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATGc                    <  1:350522/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           accaccTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATGc                    <  1:1094626/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           accaccTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATGc                    <  2:769558/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                cTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGTTCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATGCAGtaa               <  2:790478/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATTGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGCGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGCAGAACGATACCCGCGGCTTCCTGCTACGCGGGCTGCAGtaata             >  2:328920/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  gTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATg                     >  2:171617/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                  gTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATg                     <  1:171617/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cgcgGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATGCAGTAATAAATCAGCGGCt  >  2:860618/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             cgcgGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATAAACGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATAGCTTCTAGAAGAACGATCCCCGCGGCTTCCTGATACGCGGATTGCAGTAATAACTCAGCg     >  1:390362/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                         
GATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGCTATACAAATCCTGAGTATCGGCATAATGGCGAGCATTCTCCGCCCAGGCCATTCGCAATGGCGACTGAGTTAACGCTTTACGTAAAACTTCATTTAATTGTTCCTGAGAGAAAGGTTCAGCGATGACCGTTCCACAATTGGCATCCGCAATATAATGCGCGTACCCACATACCGCTGTTGTTAAAACAGGTAACCCAGCAGTGATCGCTTCTAGAAGAACGATACCCGCGGCTTCCTGATAAGCGGGATGCAGTAATAAATCAGCGGCT  >  NC_000913/3805704‑3806261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: