Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,464,611:1 +C 9.5% coding (690/1164 nt) gspL → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,464,6111.C 9.5% 155.4 / 12.7 42coding (690/1164 nt)gspLgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base . (24/14);  new base C (2/2);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

TAGATAGCTGGACTCTGGTTAACCAGCAAACAAGCTGGCTCATTCGCAGTGCCGCACATGCCTTCAACGAACTGGATGAACACTGGTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCTCAGCAATATCCTGAAGCTGTGCCGTTGCTCT  >  NC_000913/3464355‑3464870
                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                   
tAGATAGCTGGACTCTGGTTAACCAGCAAACAAGCTGGCTCATTCGCAGTGCCGCACATGCCTTCAACGAACTGGATGAACACTGGTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGaaa                                                                                                                                                                                                                                     >  1:250267/1‑289 (MQ=255)
                    aaCCAGCAAACAAGCTGGCTCATTCGCAGTGCCGCACATGCCTTCAACGAACTGGATGAACACTGGTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAg                                                                                                                                                                                                                 >  2:40668/1‑289 (MQ=255)
                         gCAAACAAGCTGGCTCATTCGCAGTGCCGCACATGCCTTCAACGAACTGGATGAACACTGGTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTaa                                                                                                                                                                                                              >  1:28429/1‑287 (MQ=255)
                                 gCTGGCTCATTCGCAGTGCCGCACATGCCTTCAACGAACTGGATGAACACTGGTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGt                                                                                                                                                                                                    >  2:37661/1‑289 (MQ=255)
                                                                            tGAACACTGGTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGct                                                                                                                                                          <  2:250267/288‑1 (MQ=255)
                                                                                    ggTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGAGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCACATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGATTTCTCATTCTCGCCATTCGTTCTTTTGGTGGCAGCCGGGGCATCGCGCTCTGGCATAc                                                                                                                                                 >  1:116069/1‑289 (MQ=255)
                                                                                     gTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATAc                                                                                                                                                 >  2:381647/1‑288 (MQ=255)
                                                                                       tACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCt                                                                                                                                              <  1:381647/289‑1 (MQ=255)
                                                                                          aGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTaa                                                                                                                                           >  2:323518/1‑289 (MQ=255)
                                                                                             aTCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGAt                                                                                                                                        <  2:28429/289‑1 (MQ=255)
                                                                                                             tCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCAt                                                                                                                                                   <  2:274242/263‑1 (MQ=255)
                                                                                                             tCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCAt                                                                                                                                                   >  1:274242/1‑263 (MQ=255)
                                                                                                                                     tGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTCTGTATTCTGCCGATATTGCATTTCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTATTTTGTTGGCAGACGGAGCATCGCGCTCTGGCACACGCTTATGATTGAAGACCAACTTCAGCAACACAAGNCAGAAACATggaa                                                                                                 >  2:33951/1‑286 (MQ=255)
                                                                                                                                        tgtAGGACCTGACGGCGTTGCAGCGGCCAATCCACTTAGTCAGCATCCTGAAATACAACCACTGAGTTTGTATTCTGCCGCTATTGCATGCCAGCTCTATGACATGCTGTATGGTATTTTTCTTAATAAGAAAACTGTAAGCAAATCGGGAAAATGGGTCGCCCGCCTGGCAGGAAGCTGTCTCGTTCGCGCCATTCTTTCTGTTGTTGGCAGCCGGAGAATCGCGCCGTGGCATACGCTTAAGATTGAAGCTCAACTTCAGCAACAACAGCAAGAAACCTGGCAACg                                                                                              <  2:389391/288‑1 (MQ=255)
                                                                                                                                               ccTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTc                                                                                       >  2:391324/1‑288 (MQ=255)
                                                                                                                                                 tCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCg                                                                                    <  1:391324/289‑1 (MQ=255)
                                                                                                                                                 tCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCg                                                                                    <  1:37661/289‑1 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCt                                                                                                                                              <  1:173366/231‑1 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCt                                                                                                                                              >  2:173366/1‑231 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCtt                                                                                                                                             <  1:411418/231‑1 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCtt                                                                                                                                             >  2:411418/1‑231 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGcaac                                                                                                                    <  1:150434/256‑1 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGcaac                                                                                                                    >  2:150434/1‑256 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGc                                                                                   >  1:343859/1‑289 (MQ=255)
                                                                                                                                                  cACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATAAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGc                                                                                   >  1:60783/1‑289 (MQ=255)
                                                                                                                                                    cGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCaacaac                                                                                                                 >  2:39539/1‑257 (MQ=255)
                                                                                                                                                    cGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCaacaac                                                                                                                 <  1:39539/257‑1 (MQ=255)
                                                                                                                                                               cGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCa                                                                       >  2:312045/1‑288 (MQ=255)
                                                                                                                                                                                   cATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGCTCAACTTCAGCCACAACAGCAAGAAACCTGGCAACGGTGTTTCCCCCAGATCAAACGCACCCGCAATTTTCGTTTTTACt                                                  >  1:207724/1‑289 (MQ=255)
                                                                                                                                                                                   cATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACt                                                  >  2:92950/1‑289 (MQ=255)
                                                                                                                                                                                                cATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACCATTTTCGTTTTTACTTTAAGCAGCAAc                                      >  2:234339/1‑288 (MQ=255)
                                                                                                                                                                                                  tCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTc                                    >  2:84292/1‑288 (MQ=255)
                                                                                                                                                                                                  tCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCg                                   <  1:40668/289‑1 (MQ=255)
                                                                                                                                                                                                    acggAGTTTGTATTCTGCCGATATTGCATTCCAGCTCTATGACATGCGGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCt                                 <  2:207724/286‑1 (MQ=255)
                                                                                                                                                                                                             gTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCTCAGCAata                         >  1:65771/1‑288 (MQ=255)
                                                                                                                                                                                                                  cTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCTCACCAATATCCTGa                   >  2:197164/1‑289 (MQ=255)
                                                                                                                                                                                                                    gCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCGTTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGTTCAACTTCAGCAACAACAACAAGAAACCTGGCAACGTTATTTCCCGCAGACCAAACGCCCTCACAATGTTCGTTTTTACTTTAAACATCAACTCGCCCAGCAAAATcattaa                  >  2:293495/1‑283 (MQ=255)
                                                                                                                                                                                                                       gATATTGCATTCCAGGGCTATGACATGCTGGATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTTGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTACCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCTCAGCAATATCCTGAAGCtg              <  2:332666/289‑1 (MQ=255)
                                                                                                                                                                                                                               aTTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCTCAGCAATATCCTGAAGCTGTGCCGtt       <  2:168996/288‑1 (MQ=255)
                                                                                                                                                                                                                                   cAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCATCAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCTCAGCAATATCCTGAAGCTGTGCCGTTGccct  >  2:174382/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     cATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACTCACCCACAATTTTCGTTTTTACTTTAAGCAGCAAc                                      <  1:50820/235‑1 (MQ=255)
                                                                                                                                                                                                                                                     cATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAAc                                      >  2:50820/1‑235 (MQ=255)
                                                                                                                                                                                                                                                             ttttCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCa                                                                                                                                                    >  2:227425/1‑117 (MQ=255)
                                                                                                                                                                                                                                                             ttttCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCa                                                                                                                                                    <  1:227425/117‑1 (MQ=255)
                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                   
TAGATAGCTGGACTCTGGTTAACCAGCAAACAAGCTGGCTCATTCGCAGTGCCGCACATGCCTTCAACGAACTGGATGAACACTGGTTACAGCATCTTGCTGCCCAGTTTCCGCCTGAGAATATGCTTTGTTATGGTGTAGTACCTCACGGCGTTGCAGCGGCCAATCCACTTATCCAGCATCCTGAAATACCATCACTGAGTTTGTATTCTGCCGATATTGCATTCCAGCGCTATGACATGCTGCATGGTATTTTTCGTAAACAGAAAACTGTAAGCAAATCGGGAAAATGGCTCGCCCGCCTGGCAGTAAGCTGTCTCGTTCTCGCCATTCTTTCTTTTGTTGGCAGCCGGAGCATCGCGCTCTGGCATACGCTTAAGATTGAAGATCAACTTCAGCAACAACAGCAAGAAACCTGGCAACGTTATTTCCCGCAGATCAAACGCACCCACAATTTTCGTTTTTACTTTAAGCAGCAACTCGCTCAGCAATATCCTGAAGCTGTGCCGTTGCTCT  >  NC_000913/3464355‑3464870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: