New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2553462147 (0.880)3 (0.020) 3/496 NT 2.0% coding (238/900 nt) hemF coproporphyrinogen III oxidase
?NC_000913 = 2553468 149 (0.920)coding (244/900 nt) hemF coproporphyrinogen III oxidase
Rejected: Frequency below/above cutoff threshold.

TTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2553173‑2553462
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcccggCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAACCAG  <  NC_000913/2553468‑2553182
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
TTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCG                                                                                                                                                                                                                                                                                                  >  1:1346589/1‑289
  ATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGATCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCCGCGGCGGGCGGAGTCGGGTGTTGAGTAATGGGGGAGTCTTCGAACAGGGAGGCGTCAACTTTTCGCACGTCACCGGTAAGGTGATGCCGGCTACCGCCACCGGTCATCGCGCGG                                                                                                                                                                                                                                                                                                 >  2:375693/1‑288
                                                                                                                                                                                                                                               CGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCAT                                                                                                                                                                                                    <  1:54339/144‑1
                                                                                                                                                                                                                                               CGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCAT                                                                                                                                                                                                    >  2:54339/1‑144
                                                                                                                                                                                                                                                                                    CGCTCATCGCCCGGCAAGTTCCGGGCGATGAGCGCTGGCGGAAGCAGGCATCGCCTCACCGTGGACAGGCGCAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGCCTACGCCCGCGGCCACCACCTACGCGATGCCAACTATATTCGCCAAATTCTGCGCCACCGACGGCGGTCGGCCGCTTACACATCGGATCCTGAAGGTTGCGCAGAAACAGTTTGACCTGGTTTGGGTCGGGTTTCATAACTTATCGCTTTTGCGAATGTGCTTACTGG              >  2:989492/1‑289
                                                                                                                                                                                                                                                                                           CGCCCGGCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGATGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCG                            >  1:1567606/1‑268
                                                                                                                                                                                                                                                                                           CGCCCGGCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGATGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCG                            <  2:1567606/268‑1
                                                                                                                                                                                                                                                                                           CGCCCGGCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGA       >  1:472881/1‑289
                                                                                                                                                                                                                                                                                              CCGGCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAAC     <  1:1099438/288‑1
                                                                                                                                                                                                                                                                                              CCGGCAAGGTCGGGGCGATGAGCGGTGGCGAAACCAGGCACCGCCTCACAGTGGACAGGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCGGACTACGCCCGCCGCCGCCAGCTTCGTTCTGCCAACTAGCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGTAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAAC     <  2:995690/288‑1
                                                                                                                                                                                                                                                                                               CGGCAAGTTCCGGGAGATGGGCTGTGGCGTAAGCAGGCATCGCCGCACCGTCGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACCCCACCATTACGCCACACCCGACTACCCCCGCCGCCGCCGGCTTCGCGCTGCCAACGAACTTCGACAAATTCTGCGCCATCGACGGCCGTCAGCTGCTGACAAATCGTATCCTGAATGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGCGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAACCA   <  2:450310/289‑1
                                                                                                                                                                                                                                                                                               CGGCAAGTGCCGGGCGATGAGCGGGGGCGGAAGCAGGCATCGCCTCACCGTGGACAGGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAACCA   <  1:1069734/289‑1
                                                                                                                                                                                                                                                                                               CGGCAAGGTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGACTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAACCA   <  2:391525/289‑1
                                                                                                                                                                                                                                                                                                GGCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAACCAG  >  1:64948/1‑289
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
TTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCGATGGCGCAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2553173‑2553462
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcccggCAAGTTCCGGGCGATGAGCGGTGGCGGAAGCAGGCATCGCCTCACCGTGGACATGCGAAAAGTTGACGCCTGCCTGTTCGAAAACACCACCATTACGCAACACCCGACTACGCCCGCCGCCGCCAGCTTCGCGCTGCCAACTATCTTCGACAAATTCTGCGCCATCGACGGCGGTCAGCTGCTGACAAATCGTATCCTGAAGGTTGAGCAGAAACTGTTTAACCTGGTGTGCGTCGGGTTTCATAACTTATCGCTTTTTCGAATGTGCTTTCTGGTTGTCGAACCAG  <  NC_000913/2553468‑2553182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.