New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4522458 =155 (1.070)4 (0.030) 4/500 NT 2.9% coding (1637/1968 nt) yjhG putative dehydratase
?NC_000913 4522501 = 122 (0.880)coding (1594/1968 nt) yjhG putative dehydratase
Rejected: Frequency below/above cutoff threshold.

TTCGGTGATCAAATCCACCGCCATTGACCCCTCGATGATTGATGAGCAAGGTATCTATTACCATAAAGGTGTGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCATCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4522751‑4522458
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtgcatcggTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGA  >  NC_000913/4522501‑4522785
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
TTCGGTGATCAAATCCACCGCCATTGACCCCTCGATGATTGATGAGCAAGGTATCTATTACCATAAAGGTGTGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCCGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAATCATCTGTCATACGGTAAGCATGTTTCGTTACTCACCGATGCACGTTTCCCGGGCGTTTCTCATGGCCCGTGC                                                                                                                                                                                                                                                                                                    >  2:585373/1‑289
TTCGGTGATAAAAGCCACCGCCATGGACCCCGCGATGATGGATTAGAAAGGTATCTATGACCATAAAGGGGGAGCGAAGGTTTAACTGTTCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATGAAGGCGGGCGATATTCGGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGTTTACCAGTGCCCTGAAGCATCTGTCATACGGTATGCATGTTTCGTTAATCACCGATGCACGTGTCTCGGGCGTTTCTACTGGCGCGTG                                                                                                                                                                                                                                                                                                     <  2:862606/288‑1
 TCGGTGATCCATTCCACCGCAATTGACCCCTCGAATATTGATGAGCAAGGTATCTTTTACCATAAAGGTGTGGCGAAGGTTTATCTGTCCGAGGAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCA                                                                                                                                                                                                                                                                                                   <  2:145738/289‑1
 TCGGTGATCAAATCCACCGCCATTGACCCCTCGATGATTGATGAGCAAGGTATCTATTACCATAAAGGTGTGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCA                                                                                                                                                                                                                                                                                                   <  1:226829/289‑1
 TCGGTGATCAAATCCACCGCCATTGACCCCTCGATGATTGATGAGCAAGGTATCTATTACCATAAAGGTGTGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGC                                                                                                                                                                                                                                                                                                    <  2:1067242/288‑1
                                                               TAAAGGTGTGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCATCGGTAATTAACGAAACATGCTTACCGTATGACCGATGCTTCAGGTCCCACGTAACCTGGTA                                                                                                                                                                                                                                     >  1:387216/1‑289
                                                                       TGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCA                                                                                                                                                                                                                                                                                                   >  1:849590/1‑219
                                                                       TGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCA                                                                                                                                                                                                                                                                                                   <  2:849590/219‑1
                                                                          tGAAGGTTTATCTACCCGAGAAAAGTGTGATTTACGGTATCAAACATGACAAGGTCAAGGCTGGATATATTCTGGCAATTATTGGCGTTGGACCTTCAGGTACAGAGATGGAAGAAACCCACCAGGTTACCAGTGCCCTGAAGCATCTGTCAGACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGCGCGTTTCTACTGGCGCGTGCATCGGTGATTAACGAAACATGCTTACCTTATTACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCC                                                                                                                                                                                                                           <  2:387216/287‑1
                                                                                                                                         GCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGG                                                                                                                                                                                                                                                     <  1:518703/199‑1
                                                                                                                                         GCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGG                                                                                                                                                                                                                                                     >  2:518703/1‑199
                                                                                                                                                                         TCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGC                                                                                                                                                                                                                                                                                                        >  1:669659/1‑116
                                                                                                                                                                         TCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGC                                                                                                                                                                                                                                                                                                        <  2:669659/116‑1
                                                                                                                                                                                                                                                                                           acggGCATCCGTGATTAACGAAACATGCTTACCGTATGACAGAGGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGG         <  2:526494/285‑1
                                                                                                                                                                                                                                                                                              TGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGTAATATTGTCACCCGGGAAG      >  1:1200267/1‑289
                                                                                                                                                                                                                                                                                              TGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAG      >  2:180195/1‑289
                                                                                                                                                                                                                                                                                              TGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACC            <  2:264476/283‑1
                                                                                                                                                                                                                                                                                              TGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACC            >  1:264476/1‑283
                                                                                                                                                                                                                                                                                                CATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATACCCGAACCTTTTGGCGCActctttccta              >  2:222675/1‑269
                                                                                                                                                                                                                                                                                                  TCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGAAAGGTGA  <  1:28166/289‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
TTCGGTGATCAAATCCACCGCCATTGACCCCTCGATGATTGATGAGCAAGGTATCTATTACCATAAAGGTGTGGCGAAGGTTTATCTGTCCGAGAAAAGTGCGATTTACGATATCAAACATGACAAGATCAAGGCGGGCGATATTCTGGTCATTATTGGCGTTGGACCTTCAGGTACAGGGATGGAAGAAACCTACCAGGTTACCAGTGCCCTGAAGCATCTGTCATACGGTAAGCATGTTTCGTTAATCACCGATGCACGTTTCTCGGGCGTTTCTACTGGCGCGTGCATCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4522751‑4522458
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgtgcatcggTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGAATATCGCCCGCCTTGATCTTGTCATGTTTGATATCGTAAATCGCACTTTTCTCGGACAGATAAACCTTCGCCACACCTTTATGGTAATAGATACCTTGCTCATCAATCATCGAGGGGTCAATGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGA  >  NC_000913/4522501‑4522785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.