Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,489,443 Δ1 bp 6.4% coding (738/1221 nt) argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,489,4430A. 6.4% 168.0 / 4.3 62coding (738/1221 nt)argDbifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base . (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

ATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGATCATCCATCACCGCTTTCACTGCATGGAGATCGTTAA  >  NC_000913/3489218‑3489663
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             
aTGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGGCAGAATATCCGCCGTAAc                                                                                                                                                                                                                             >  1:414827/1‑228 (MQ=255)
  ggTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCGGAGGATTACCGCCGTAGGTGGAACCGTGAAAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAACCGCCGCCTAACGCTTTCCCAAAGGGCCAAATAACCGGCGAAAccc                                                                                                                                                                                                                           >  2:295391/1‑226 (MQ=255)
  ggTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGGGCTAATCGGGAAGCCCCCGCCCAACCCTTCGCCCAGGGTCAGAATATCCGGCGTAAccc                                                                                                                                                                                                                           >  2:427053/1‑226 (MQ=255)
  ggTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGCGGACCCCTGAGAACCAGGATGAAACGCAGAAGCCATTTCAGCCGTGGCCCGCATTGCGCTACATGGGCAGCCACCGCATAACGCTCTCGCAGAGTACACAATATCCGCCGAAAccc                                                                                                                                                                                                                           >  2:253877/1‑226 (MQ=255)
     tcaaaaaagccTGGGGTTTCGCCCGTAGGCCTCTCCAGCCCGTAGGGGGATGTGATGCATACAAGCGCCCCCCCCGAAACTACCCGGGCAAGGGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCg                                                                                                                                                                                                                         <  2:170604/218‑1 (MQ=255)
                              aaTGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGCAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGACTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGCAGTGCACGTAAGCAAACAAAtaga                                                                                                                                                                                               >  2:53618/1‑224 (MQ=255)
                                 gCCTTCCAGATCTTAAGGGGTAGTGATGAAACAAAGCGCCCCCCCCGCTACTGCAAAGGCCAGAGTATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGt                                                                                                                                                                                           <  1:613182/228‑1 (MQ=255)
                                         aGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGCGGAAACGCAGAAGCAATTTCCGCCGTGGCCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGGAGGAATAACCGGCGTACCCCCGCAGAGAAAGTACGCAAACAAAACGCCGGGCCGcccc                                                                                                                                                                                    >  2:656762/1‑228 (MQ=255)
                                            ccTTGAGGGGTATTGATGATATCAACCGCCCCCCCCGCTACGGCACAGGCAAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATc                                                                                                                                                                                 <  1:427053/227‑1 (MQ=255)
                                                cAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAACCGCCGCCTAACGCTTTCGCAGAGGCCAGAATATCCTGCGTAACGCCGTAGTGCATGTAAGCAACCAAATCGCCGGTCCGCCCCATCCCgc                                                                                                                                                                             >  1:243373/1‑228 (MQ=255)
                                                        ttGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTa                                                                                                                                                                                                                               >  1:475100/1‑171 (MQ=255)
                                                        ttGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAACGCAAAAAATGCCCGGTCCCCCCCAccccccccggcaat                                                                                                                                                                     >  2:549007/1‑218 (MQ=255)
                                                                  atCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCaa                                                                                                                                                              >  1:32669/1‑227 (MQ=255)
                                                                  atCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCaa                                                                                                                                                              >  1:19118/1‑227 (MQ=255)
                                                                  atCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCaa                                                                                                                                                              >  1:5530/1‑227 (MQ=255)
                                                                         gccgccCCCGCTACTGAAAGGGCCAGAGGATTCCCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCaat                                                                                                                                                      <  1:387335/228‑1 (MQ=255)
                                                                          ccgccCCCGCAACCGCAAAGGCAAGCGGATGCCCGCCTTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCaata                                                                                                                                                     <  2:281028/228‑1 (MQ=255)
                                                                            gccCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGCACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCTGCGCCTAACGCTTTCGCAGAGGTCCGACTATCCGGCGTAACGCCGTAGGGCATGTAACCAAACAAATAGCCGGTCCCCCCCACCCCCCCACGCACTTCATCAAACACCaaaaat                                                                                                                                                   >  2:672315/1‑228 (MQ=255)
                                                                             cccccGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCCTCCCGCCCTGCACTCCATCAACCACCaatcat                                                                                                                                                   >  1:593463/1‑227 (MQ=255)
                                                                                         cacaGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGctgcctcttat                                                                                                                                       >  1:93146/1‑217 (MQ=255)
                                                                                          acaGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGAt                                                                                                                                      >  1:480845/1‑227 (MQ=255)
                                                                                              gCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGcac                                                                                                                                 >  1:457853/1‑227 (MQ=255)
                                                                                                cAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAg                                                                                                                               <  1:257935/228‑1 (MQ=255)
                                                                                                 aggggATTCCCGCCGTGGGTGGACCCGGGCGAACCCGGATGAAACCCGCAAGCAATTTCCGCCGTGGGCAGCATGGCCCTAATCGGGAAGCCGCCGCCTAACGCTTACGCAGAGGTCAGAATAGCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGc                                                                                                                              <  2:32669/225‑1 (MQ=255)
                                                                                                 agactaTTACCCCCGGGGGGTGAGCCGGGAGACCCAGGATGAAACGCGAAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGc                                                                                                                              <  2:601951/223‑1 (MQ=255)
                                                                                                   agGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGCAAGCAAACAAATCGCCGGTCCCCCCCATCCCCCACTGCACTTCTTCAAACACCAAAAATGCCTGATGTTGATCGCACACCt                                                                                                                             >  2:375388/1‑227 (MQ=255)
                                                                                                        tACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACCGCTCGCGCa                                                                                                                       >  1:271617/1‑228 (MQ=255)
                                                                                                        tACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGACCTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCCCACCCCGCCCa                                                                                                                       >  2:441965/1‑228 (MQ=255)
                                                                                                        tACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGGAGTGCATGAAAGCAAACAAATCGCCGGTCAGc                                                                                                                                                                                       >  1:99569/1‑164 (MQ=255)
                                                                                                        tACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCCTTCGCAGCGGTCAGAATAACCGGCGTAACGCCGTATTGCATCAAAGCAAACAAATCGCCGATCCGCCCCATCCCGCACTGCACGCCATCAAACACCAATAATCCCTCCTCTTAATCCCACATCCCCCGCa                                                                                                                       >  2:212434/1‑228 (MQ=255)
                                                                                                          ccgctgggggTGGAACCGTGAGACCCAGGATGAAGCGCAGAACCATTTTCCGCCGTGGTCAGCATGGCGCTAAGCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATCTCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCaa                                                                                                                      <  2:504481/219‑1 (MQ=255)
                                                                                                            gccgTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGAgcgccagg                                                                                                                               >  1:601951/1‑212 (MQ=255)
                                                                                                               gTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACACTTACCGCAAGCCCTg                                                                                                                >  1:505349/1‑228 (MQ=255)
                                                                                                                tagtggggaCCGTGAGAACCAGGAGTAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCCGTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGccc                                                                                                                  <  2:593463/217‑1 (MQ=255)
                                                                                                                        aCCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCCCACTGCACTTCATCAAAAACCAACAATGCCTGATGTTGACCGCACAGCCCGCGCAAGCCCTCCAAAAAcac                                                                                                       >  2:41978/1‑225 (MQ=255)
                                                                                                                            tGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCCCTAATCGGGAAACCGCCGCCTAACGCTTTAGCAGAGGTCAGAATATCCGGGCTAACGCCGTCGTCCATGTAACCAAACAAGTCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAACACCCACTAAGGCCCGATGTTTATCGCCCAGCTACCGCCAGACCCCCaaaacactagc                                                                                                    >  2:98036/1‑220 (MQ=255)
                                                                                                                                  ccAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGca                                                                                                                                  <  1:4204/191‑1 (MQ=255)
                                                                                                                                  ccAGGATGAAACCGGAAAGCATTTTCCGCCGTGGGCAGCATGGCGCTAACCGGGAAGCCGCCGCCTAACGCTTCCGCAGAGGGCAGAATTCCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGc                                                                                              <  2:37723/227‑1 (MQ=255)
                                                                                                                                  ccAGGATGAAACCCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGc                                                                                              <  1:655000/227‑1 (MQ=255)
                                                                                                                                                aGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGAGCCTGTAAGCAAACAAATCGCCGTTCCGCCCCATCCCGCACTGCACTTCATCAAACACAAATAATGCCTGATGCTGCTCGCACAGCTCGCCCAAGCCCCCCAAAAAACCTCGCGTCGCTGCCCTCACCCctcc                                                                               >  2:71705/1‑228 (MQ=255)
                                                                                                                                                              gcgTGGTCAGCATGGCCCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGa                                                                    <  1:641851/224‑1 (MQ=255)
                                                                                                                                                              ccgTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGGTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGa                                                                    <  1:490381/225‑1 (MQ=255)
                                                                                                                                                                        cATGGCGCTAAACGGGAAGCCGCCGCCTAACGCTTTCGCAGGGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAaccac                                                       <  1:672315/228‑1 (MQ=255)
                                                                                                                                                                          ttgcctctaccGGGACGCCCCCGCCAACCGCTTTCGCCAAGGTGAGAATACCCCGCCTAACTCCGTCGTGCAATTAAGCAAACAAATCGCCGGTCCTCCCCATCCCGCCCTGCACTTCATCAAACACCAATAATGCCGGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAaccacca                                                     <  2:243373/218‑1 (MQ=255)
                                                                                                                                                                          tggggttAATCGGGAAACCGCCGCCTAACGTCTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAaccacca                                                     <  2:5530/222‑1 (MQ=255)
                                                                                                                                                                          tGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGGCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAaccacca                                                     <  1:375388/228‑1 (MQ=255)
                                                                                                                                                                          gggCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAaccacca                                                     <  1:656370/227‑1 (MQ=255)
                                                                                                                                                                             cgcTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTACGCCGGAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCg                                                  <  1:41978/227‑1 (MQ=255)
                                                                                                                                                                              gcTAAGCGGGAACCCGCCGCCTAACGCTTTCGCAGAGGTCAGAAGATCCGGCGTAACGCCGTAGTGCATGGAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACGTCATCAAACACCAATAATGCCGGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCTCCACCg                                                  <  2:166875/227‑1 (MQ=255)
                                                                                                                                                                                tAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGca                                                <  1:295391/227‑1 (MQ=255)
                                                                                                                                                                                tAATCGGGAAGCCCCCGCCTAACGCTTTCGCAGAGGGCAGAATACCCGGCGTAACGCCGGAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAACCTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGca                                                <  1:98036/227‑1 (MQ=255)
                                                                                                                                                                                tAAGCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGca                                                <  1:53618/227‑1 (MQ=255)
                                                                                                                                                                                  aTCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGccca                                              >  1:393170/1‑227 (MQ=255)
                                                                                                                                                                                       gAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGAATGTAAGCAAACAAATCGCGGGTCAGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACACCCCTGCCCCTGGATCGCACCAACCACCACCGCACAGgagt                                         >  1:57859/1‑227 (MQ=255)
                                                                                                                                                                                         accccccgccTAACGCTTTGCGCGAGGTCAGGATCTCCCGCGTAACGCCGTAGTTCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCCCTTCAACAAACACCAATAATGCCTGATGTTGAGCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTAGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGa                                       <  2:19118/222‑1 (MQ=255)
                                                                                                                                                                                         aGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGa                                       <  1:441965/227‑1 (MQ=255)
                                                                                                                                                                                                  cTAACGCTTTCGCAGAGCTCAGAATACCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAACACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGATcatccatc                              <  1:71705/227‑1 (MQ=255)
                                                                                                                                                                                                         cttccgaaaagGTCCGAAAGTCCGGCGTACGCCGTAGGTCATGTAAGCAAACAAACCGCCGGTCCCCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGATCATCCATCACCGCttt                      <  2:457853/219‑1 (MQ=255)
                                                                                                                                                                                                           tCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGATCATCCATCACCGCTTTCa                    <  1:506958/228‑1 (MQ=255)
                                                                                                                                                                                                                        ggAATATCCGGGGTAACGCCGTAGTGCATGTAAGCAAACAAATCCCCGGTCCGCCCCCTCCCGCACTGCACTTCATCAAACCCCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGATCATCCATCACCGCTTTCACTGCATGgaga         <  1:212434/227‑1 (MQ=255)
                                                                                                                                                                                                                               ccGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCCCCGCCCAGGTGTGACCCTCCATCCCCGCTTTACCTCCACGGAAATCGCTaa  >  2:112736/1‑228 (MQ=255)
                                                                                                                                                                                                                               ccGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGATCATCCATCACCGCTTTCACTGCATGGAGCTCGTTaa  >  1:579559/1‑228 (MQ=255)
                                                                                                                                                                                                                               ccGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCCGGATCGGCTCAACCACCACCGCACAGGGGTGGTCATCCCTCACCGCTTTCACTGCCCGGAAATCGCTaa  >  2:479189/1‑228 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                             
ATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGCCGGTCCGCCCCATCCCGCACTGCACTTCATCAAACACCAATAATGCCTGATGTTGATCGCACAGCTCGCGCAAGCCCTGCAAAAACTCTGGCGTCGCTGCCGTCACACCGCCCTCGCCCTGGATCGGCTCAACCACCACCGCACAGGTGTGATCATCCATCACCGCTTTCACTGCATGGAGATCGTTAA  >  NC_000913/3489218‑3489663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: