Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 149,436 A→G 7.0% S56P (TCC→CCC)  panB ← 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913149,4360AG 7.0% 200.6 / 2.1 75S56P (TCC→CCC) panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (24/42);  new base G (4/3);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGG  >  NC_000913/149212‑149661
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
ggCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCCGGCGGTTTGGTGCGCCGCCACGTACGGCGGCAGGCTGGGAGGCGGAATCGGCAACGGTGACTGGGAGGGGGGGGt                                                                                                                                                                                                                                 >  2:185074/1‑227 (MQ=255)
ggCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCCGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGGAGGCGATAGCGGCAACGGGAACGTGCAGGGGGGAGt                                                                                                                                                                                                                                 >  1:612564/1‑227 (MQ=255)
 gCACGTTCGGTCAGCATTTGTACGGCTTCTACCAGCCACTCACCGCCTTCCATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCGGTTTGGTGCGCCGCGACGAACGGCGGCCGCTGGGTACGCGCTACCTGCAACGGCGACCGGCCGGGCGGGGTCg                                                                                                                                                                                                                               >  2:201679/1‑228 (MQ=255)
 gCACGTTCGGTAAGCATTTGTACGGTTTCTCCCAGCCACTCACCGCCTTCAATTTTGCCCATGTTAGCACCGGCACGCATAACCGTGGCGGCGTTTTCGAAGGCTTGGTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCg                                                                                                                                                                                                                               <  1:558221/228‑1 (MQ=255)
           tcacaaTTTGTACGTGTTCTACCGGCCACCCACCTCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCTTTGCGGCGTTTTCGAAGGCTTGGTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGaa                                                                                                                                                                                                                     <  1:405064/223‑1 (MQ=255)
           tCAGCTTTTTTCCGGTTTCGCCCCGCCACTCTCCGCCTTCAATTTTGACCCTGTTAGCCCCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGaa                                                                                                                                                                                                                     <  2:177705/228‑1 (MQ=255)
                           ttCTACCAGCCACTCACCGCCTTCAATTGTGACCAGGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCg                                                                                                                                                                                                      <  1:66168/227‑1 (MQ=255)
                            tCTACCAGCCACTCACCGCCTTCAATTTTAACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCACCGGTAACGGGCAGGGTGGAGTGGTGCCCCGGAACCGTCATGCCCAGCGa                                                                                                                                                                                                     >  1:684843/1‑227 (MQ=255)
                                 cAGCCACTCCCCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGcc                                                                                                                                                                                               <  1:43703/228‑1 (MQ=255)
                                    ccACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCCGCCGCAGGCAGTTTGGTGCGCCGCGACGTCCGGCGGCAGGGTGGTAGGCGAAATCGGCAACGGCAACTGGCAGGGCGGAGTCGTGCCCCTGAACCGCCCGGCCCAGCGAATCGccccc                                                                                                                                                                                            >  2:506397/1‑226 (MQ=255)
                                    ccACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGGTTGGTGCGCCGCGACGTACGGCGGCAGTGGGGTAGGCGATGTTGGCGACGGGAACTGGCGGGGGGGGAGCGGGCCCCTGAACCCTCAGGCCCCGCAAAACccccca                                                                                                                                                                                            >  2:597036/1‑226 (MQ=255)
                                         ccccGGCTTTAATTTTGACCCTGTTTGCCCCGTGACGCATACCCGTGGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATGCGCCATAAACGGCAGGGCAGCCAGCAGCAGGAAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCa                                                                                                                                                                                       <  2:140987/226‑1 (MQ=255)
                                         cACCGCCTTCAATTTTGGCCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTGTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCa                                                                                                                                                                                       <  1:113801/228‑1 (MQ=255)
                                           ccGCCTTCAATTTTGACCATGTTTGCCCCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGGTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATg                                                                                                                                                                                     <  1:597036/228‑1 (MQ=255)
                                           ccGCCTTCAATGTTGACCATGTTGGCACCGGCACGCATAACCGTTGCGGCCTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCGTAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATg                                                                                                                                                                                     <  1:289445/228‑1 (MQ=255)
                                              ccTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCCGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGGACCTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGTCCAGCGAACCGCCCACCAGCATGACg                                                                                                                                                                                  >  1:641715/1‑228 (MQ=255)
                                                 tCAATTTTGACCATGTTAGCCCCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGGTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTa                                                                                                                                                                               <  1:54154/228‑1 (MQ=255)
                                                                ttAGCCCCGGCTCGCATAACCGGTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCa                                                                                                                                                                <  1:229456/228‑1 (MQ=255)
                                                                         gCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGGGGAGGCGTGCCCCTGGAACGTCCTGCCCCGGCAATCGCCCACCAGCATGGCGTTAAGCCCCTCCTCAACAAAAAGGTTg                                                                                                                                                       >  2:482885/1‑228 (MQ=255)
                                                                         gCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGCGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAAAGCTTg                                                                                                                                                       >  1:278855/1‑228 (MQ=255)
                                                                         gCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGAACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGGGGAGTCGTGCCCCTGAACCGCCATGCCCAGCGAATCGCCCACCAGCATGACGGTAAGCCCTTCATCAGCAAAACGTTTg                                                                                                                                                       >  1:595109/1‑228 (MQ=255)
                                                                         gCACGCATAACCGCTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGCGGTAGGCGATATCGGCAACGGAAACTGGCAGGGTGGAGTCGGGCCCCGGAACCGGCATGCCCCGGGGAACGCACCCCAGCAGGCCGTTAAGCCCTCTATAAGCCAAGAGTTTg                                                                                                                                                       >  2:105364/1‑228 (MQ=255)
                                                                          cACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTgg                                                                                                                                                      <  1:651875/228‑1 (MQ=255)
                                                                          cACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGGAGGCGATATCGGCAACGGTAACTGGCAGGGGGGCGTCGTGCCCCTGAACCGTCATGCCCCGCGAATCGGCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTg                                                                                                                                                       >  1:238707/1‑227 (MQ=255)
                                                                           ccGCATAACCGTTGGGGCGTTTTCCGAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGTAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGc                                                                                                                                                     <  1:185074/227‑1 (MQ=255)
                                                                             gCATAACCGTTGCGGCGTGTGCGAGGGCTTGGTCCGGCGTGGCAGACGCCATAAGCGGGAGGTCAGCCAGAAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGa                                                                                                                                                   <  1:329267/228‑1 (MQ=255)
                                                                              gacccccGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGGGGCATACGCCAGAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGa                                                                                                                                                   <  1:1712/222‑1 (MQ=255)
                                                                                    cttggcggggTTTTCGAAGGGTTGTTCCGGGGTGGCATACGGCCTAAGCGGCAGGTCAGCCGGCAGCAGGAAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCAAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCtata                                                                                                                                            <  2:581014/224‑1 (MQ=255)
                                                                                     gTTGCCGCGTTTTCGAAGGCTTGTTCCGGCGTGGCCTGCGCCATAAACGGCAGGTCAGGCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCtata                                                                                                                                            <  1:589278/227‑1 (MQ=255)
                                                                                         cggcgTTTTAGAAGTGTAGTTGCGGGTGTGGATCCCCCAGAAACGGCAGGTCAGCCGGCAGCCGGCAGTTGGGGTCGCCGCGACGTACGGGGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCTCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCAt                                                                                                                                       <  2:165121/228‑1 (MQ=255)
                                                                                           gcgTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACAAGCATGACGGTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCCATAGTCAGaa                                                                                                                                     >  1:77611/1‑228 (MQ=255)
                                                                                                ttCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGGGGGGTAGGCGATATCGGCAACGGAAACTGGCCGGGCGGCGGCGTGCCCCTGAACCTTCATGCCCAGCGCAACGCCCACCAGCCTGACGTTAAGCCCTTCATCCGCAAACAGATTTGCGCAGCTATCGTCATAAACggg                                                                                                                                >  2:506665/1‑227 (MQ=255)
                                                                                                  cGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGa                                                                                                                              <  1:542885/228‑1 (MQ=255)
                                                                                                           gTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGTGGAAGCTATAGTCATAACCGGGGATGGTcccgt                                                                                                                     >  1:671688/1‑227 (MQ=255)
                                                                                                           gTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATCGTCATAAGCGGGGATGGTCCCGa                                                                                                                     >  1:376127/1‑228 (MQ=255)
                                                                                                                 gCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGtt                                                                                                               >  1:448172/1‑228 (MQ=255)
                                                                                                                                  aaaCGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGGGGAGTCGGGCCCCTGAACCGCCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCCTACGCAAAGAGTTGGGCGAAGCTATAGTCATAAGGGGTGATGGGCGCGAAACGGTTTTTTTCCTGGTTGTa                                                                                               >  2:654711/1‑228 (MQ=255)
                                                                                                                                   aaCGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTa                                                                                               <  1:224319/227‑1 (MQ=255)
                                                                                                                                        cAGGTCAGGCAGGATGAAGCAGGTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTg                                                                                         <  1:139717/228‑1 (MQ=255)
                                                                                                                                        cAGGTCAGCCCGCAGCAGGCAGTTTGGGTCGCCGCGACGTACGGCGGCAGTGGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACTTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTg                                                                                         <  1:691213/228‑1 (MQ=255)
                                                                                                                                          ggTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGGCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAACCGTTTTTTTTCCTGTTTGTACTTCTGcg                                                                                       >  1:592260/1‑227 (MQ=255)
                                                                                                                                           gtgagccagcAGCAGGGAAGTGGGTGCGCCGCGACGTACGGCGGGAGTTTGGTAGGCGATATCGGCCACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGACCCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAg                                                                                      <  1:676851/225‑1 (MQ=255)
                                                                                                                                           ggtagccgggAGCGGGCAGTTGGGGCCGCCGCGACGTACGGCGGCATTGCGGTAGGCGACATCGGCAACGGTAACTGGCAGGGTTGAGGCGTGCCCCTGAACCGTCATGCCCTGCGAATCGCCCACCAGCATGACGTTAAGTCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAg                                                                                      <  2:609448/225‑1 (MQ=255)
                                                                                                                                           gccggccaggAGCCGGCAGGTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAg                                                                                      <  1:654711/224‑1 (MQ=255)
                                                                                                                                                ccagcAGCAGGCAGTTTGGTGCCCCGCGACGTGCGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAgg                                                                                 <  1:258151/228‑1 (MQ=255)
                                                                                                                                                          gcagTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATAACGGCAACCGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCTAATCGCACACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt                                                                       <  1:576780/228‑1 (MQ=255)
                                                                                                                                                           aggTTGGGTGCGCGGGAGGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt                                                                       <  1:201679/224‑1 (MQ=255)
                                                                                                                                                            agTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt                                                                       <  1:112694/226‑1 (MQ=255)
                                                                                                                                                            agTTTGGGGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt                                                                       <  1:386589/226‑1 (MQ=255)
                                                                                                                                                            agTTGGGTGCGCCGGCACGTACGGCGGCAGTTGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt                                                                       <  1:482885/226‑1 (MQ=255)
                                                                                                                                                              tttGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTtc                                                                                                                                                                       >  2:20605/1‑128 (MQ=255)
                                                                                                                                                               ttGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTtca                                                                                                                                                                      <  1:20605/128‑1 (MQ=255)
                                                                                                                                                                 ggTGGGGCGCGAGGTTGGGGGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTc                                                                <  2:361383/228‑1 (MQ=255)
                                                                                                                                                                  gTGCGCCGCGACGGACGGCGGCAGTGTGGGAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTc                                                                <  1:690298/227‑1 (MQ=255)
                                                                                                                                                                    gcgcCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTACCTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCCTCATCAGCCAACGGTTTGGACAAGCAATAGTCATACGCGGGGGTGGTCGCCAAACGTTTTTTTTCCTGATTTTACTTCTGCAGTAAGGAGACGGTGGCAGGTTTCTTa                                                             >  2:285278/1‑228 (MQ=255)
                                                                                                                                                                    gcgcCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCGGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATa                                                             <  2:448172/228‑1 (MQ=255)
                                                                                                                                                                      cccggGACGTCAGGCGGCAGTGGGGTAGGCGATAGCGGAAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATaa                                                            <  1:674804/226‑1 (MQ=255)
                                                                                                                                                                        cgcgACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGGGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCt                                                                                                                                               >  1:466266/1‑142 (MQ=255)
                                                                                                                                                                        cgcgACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTACTGTTTGTAATTCTGCAGTAAGGGGATGGTGGTAGGTTTCATAACGt                                                         >  1:222922/1‑228 (MQ=255)
                                                                                                                                                                         gcgACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGGGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCta                                                                                                                                              <  2:466266/142‑1 (MQ=255)
                                                                                                                                                                           gacggtaggcggcAGTGTGGTAGGCGATACTGGAAACGGTAACTGGCAGGGTGGAGTAGTTCCCCTGAACCGTCATGCCCAGAGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATc                                                      <  1:683381/221‑1 (MQ=255)
                                                                                                                                                                                    gggcAGTGGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAAt                                             <  1:85231/227‑1 (MQ=255)
                                                                                                                                                                                         gtgtGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTCTAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGTTAAATTg                                           >  1:695802/1‑225 (MQ=255)
                                                                                                                                                                                               tAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg                                   <  1:506397/227‑1 (MQ=255)
                                                                                                                                                                                               tAGGCGAGATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCTCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATATTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg                                   <  1:178565/227‑1 (MQ=255)
                                                                                                                                                                                                 ggCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGAtgttgt                                    >  1:106410/1‑224 (MQ=255)
                                                                                                                                                                                                  gCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg                                   <  2:106410/224‑1 (MQ=255)
                                                                                                                                                                                                      tatCGACAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTAATGCCCAGCGAATAGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGCcgc                                                                                                                       >  1:268082/1‑136 (MQ=255)
                                                                                                                                                                                                      gagcGGCAACGGTAACTGGCAGGGGGGAGTGGTGCCCATGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCATTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTc                              <  2:376127/222‑1 (MQ=255)
                                                                                                                                                                                                               cGGTTACTGGCAGGGGGGGGTCGTGCCCATGAACCGTCAGGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCAGCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTAt                   <  1:105364/227‑1 (MQ=255)
                                                                                                                                                                                                                ggTAACTGGGCGGGTGGGGTCGGGCCCCTGAACCGTCATGGCCAGCGAAGTCCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCa                 <  2:42112/228‑1 (MQ=255)
                                                                                                                                                                                                                          aGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATc                  >  1:42112/1‑217 (MQ=255)
                                                                                                                                                                                                                            ggTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAACAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAAAGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCGGTCTGGCATTTTATCAGTCACATtggtg     >  1:255463/1‑228 (MQ=255)
                                                                                                                                                                                                                              aggAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCCTCCGCAAAGAGTTTGGCACACCTATAGTCATAAGCGGTGATGGTCGCGCAACGTTTTTTTTCCTGt                                                                                                    >  2:60297/2‑131 (MQ=255)
                                                                                                                                                                                                                                gAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTgggg  >  1:359634/1‑227 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
GGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGG  >  NC_000913/149212‑149661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: