Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,424,240 G→T 32.9% L153F (TTG→TTT trkG → Rac prophage; potassium transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,424,2400GT32.9% 31.9 / 3.6 23L153F (TTG→TTTtrkGRac prophage; potassium transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (4/11);  new base T (6/2);  total (10/13)
Fisher's exact test for biased strand distribution p-value = 3.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGGATTTTGTTTTCTGTTATTAGTGCATTCCCTTTATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGTTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTTCTCAACT  >  NC_000913/1424016‑1424465
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                  
ttttGGATTTTGTTTTCTGTTATTAGTGCATTCCCTTTATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCa                                                                                                                                                                                                                                 <  1:106819/228‑1 (MQ=255)
          tGTTTTCTGTTATTAGTGCATTCCCTTTATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTTTTGTTTTTCTATTTATTggga                                                                                                                                                                                                                       >  2:582224/1‑227 (MQ=255)
                             aTTCCCTTTATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACACCAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGCGTTATTGTTCTGGCGGTTGGTGTATTGCCATTATTGGGGGTTGGTGGTGCTAAGCtttt                                                                                                                                                                                                   >  1:100503/1‑227 (MQ=255)
                               tCCCTTTATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTTGCCTTTTTGGGGTTTGTTGGTGCTAAGtttttt                                                                                                                                                                                                  >  1:236322/1‑225 (MQ=255)
                               tCCCTTTATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCGGGCGGTTGCGTATTTGTATTTATTGTGGATTTGGGGTGCAAAGCgttctt                                                                                                                                                                                                 >  1:97745/1‑225 (MQ=255)
                                    ttATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTGGTTGTATTGCCCTTATTTGGGATTGTTGGGTCGAAGCTTTTtcagtct                                                                                                                                                                                            >  1:632212/1‑227 (MQ=255)
                                               cctaggaCTTAATTTAACGTTCATTGATTCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCggg                                                                                                                                                                                 <  1:582224/222‑1 (MQ=255)
                                                       ttAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTa                                                                                                                                                                         <  1:447593/228‑1 (MQ=255)
                                                                cGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTTCTGTATTTTACTTTTTTGGTATTGGTGGTGCAATGCTGTTTTATTCTGAAAAGACCGGGGCGTTTAAGGATGAc                                                                                                                                                                 >  2:38577/1‑227 (MQ=255)
                                                                    cATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTTTCATTATTGGGGGATGGTTGGTCAAAGCTTTATTAGTCCGGAATGCCGGGGCCATTTAATGATGACAAac                                                                                                                                                             >  1:319631/1‑227 (MQ=255)
                                                                        tattttttttttGAAGGGGTTTCTGGAAAAACAACAACAGGAGCAACTTTAATTGATGAGGTTAGTTTATTGCCTGGGGCATGTTTGTTTTTTCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCGGGCGGTTGATGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAactcact                                                                                                                                                        <  2:100503/219‑1 (MQ=255)
                                                                                           tttCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTTcc                                                                                                                                                                                                                                  >  2:208118/1‑136 (MQ=255)
                                                                                            ttCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCa                                                                                                                                                                                                                                 <  1:208118/136‑1 (MQ=255)
                                                                                                                     aCTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCt                                                                                                           <  1:38577/228‑1 (MQ=255)
                                                                                                                         ttaTTGATGTTTTTATTTTATTTCCTGGGGCATTTTTTTATTTTCGGTCAAAGTTAAATTTTATAGGTGGTTTAGGATTTATTGTTCTGGGGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTttat                                                                                                       <  2:38644/226‑1 (MQ=255)
                                                                                                                            ttGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTttatt                                                                                                      <  1:484830/226‑1 (MQ=255)
                                                                                                                                 gatgTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTttatta                                                                                                     <  1:95131/222‑1 (MQ=255)
                                                                                                                                            aTTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGt                                                                                    <  1:94947/228‑1 (MQ=255)
                                                                                                                                                               tACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTTCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCCGTCAGAAATGCCGGGGCCATTTTAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGGAACTTTGGATAAATTATTTTTTATTAGGGATTGCTTTGAATGGCTGTTATAGACttttcgga                                                                 >  1:153648/1‑222 (MQ=255)
                                                                                                                                                                    tCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGGTTTATTAGTTAGAAATGCCGGGGCCATTTAAGGATGGCAAACTCACTCCCCCCCTGGCCGCTACGCCACGGACACTGTGGGGAACTTATTCTTTATTTTGTGTTGGTTGTATTGTCTGTTATTTACTTTCAGGGATgtc                                                            >  2:494607/1‑226 (MQ=255)
                                                                                                                                                                                          ggttgtTTTGGTGTTTTTTTTCTTGCTGTTGCTGTATTTCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAATCTACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGTTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCAc                                      <  2:153648/228‑1 (MQ=255)
                                                                                                                                                                                                                          tttATTTCCATTATTGGGTTTTGGTGGTGCAAAGCTTTATCAGTCAGAAAGGCCGGGGCAATTTAAGGATGAAAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGTTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTtctc      <  2:537546/226‑1 (MQ=255)
                                                                                                                                                                                                                                tGCCATTATTGGGTTTTGGGGGTGCAAAGCTGTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGTACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGTTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTTCTCAACt  <  1:467018/227‑1 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                  
TTTTGGATTTTGTTTTCTGTTATTAGTGCATTCCCTTTATGGATTGACTCAGAACTTAATTTAACGTTCATTGATGCTCTGTTTGAAGGGGTTTCTGGAATAACAACAACAGGAGCAACTGTAATTGATGATGTTAGTTCATTACCTCGGGCATATTTGTACTATCGGTCACAGTTAAATTTTATAGGTGGTTTAGGAGTTATTGTTCTGGCGGTTGCTGTATTGCCATTATTGGGTATTGGTGGTGCAAAGCTTTATCAGTCAGAAATGCCGGGGCCATTTAAGGATGACAAACTCACTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGTTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTTCTCAACT  >  NC_000913/1424016‑1424465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: