Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,398,941 A→G 28.3% S346S (TCT→TCC)  mreC ← cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,398,9410AG28.3% 49.0 / 2.1 32S346S (TCT→TCC) mreCcell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base A (3/19);  new base G (6/4);  total (9/23)
Fisher's exact test for biased strand distribution p-value = 1.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGAT  >  NC_000913/3398719‑3399161
                                                                                                                                                                                                                               |                                                                                                                                                                                                                             
gATGGGGCAAGGCAAGGAGCCAATACAACAAGAGGGGTAACACCCAGTTGGGCCGGAAAACAATGAGGTTATCCGGGCAGGTCAGGACTTGCAGCAGCAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCTTCCCTGGCTACGATAGCTCGCCTCTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCTCTTTGTGGAGAGCg                                                                                                                                                                                                                          <  2:451240/228‑1 (MQ=255)
  tGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTTGGCACCACTTTGCGGCGGCGTA‑GCCCCTTGTGGGGAGCggg                                                                                                                                                                                                                        >  1:175955/1‑226 (MQ=255)
  tGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGCTGAGCACCCCTTTGCGGCGGCCGA‑CCCCCTTGTGGagaggggg                                                                                                                                                                                                                        >  2:31004/1‑224 (MQ=255)
        ggcgCCAGAGTCCAAGACAAAAAGAGGTGCAACACCTGGTGCGGCCGGAAAACAAGCAGGTTACCCGGCCAGGGCACGATTTGCCGCAACCGCGCAATGAGGAAAGAGAGCCGGATTACCCAGCGTCCCTGGCTGCGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTagcag                                                                                                                                                                                                                  <  2:313209/225‑1 (MQ=255)
            ccAGGTTGCAAGACACAAAGATGTGTGAAACCCTGTTTGTCCGGAAAAAAATCAGGTTGCCCGGCCAGGGCATGATTGGCAGCAACAGAGCAATGAGGAAAGAGGGGCAGATTACCCAGCGGCCCTGGCTACGATAGCTCGCCACTAGTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAgg                                                                                                                                                                                                              <  2:344914/228‑1 (MQ=255)
            ccAGGAACCAATACAACAAGAGGAGTAACCCCCATTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAgg                                                                                                                                                                                                              <  2:410387/228‑1 (MQ=255)
                     aaTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGAAAGCTCGCCACTATTGCCCTCCCGGCGCACCCGCAGGCGGGTGAGCGCCCCTCTTCGGCGGCGCACGCCCCTCGCGGGGA‑CGGTTAgcagcaccgcggggcgg                                                                                                                                                                                                     >  2:507467/1‑217 (MQ=255)
                            caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTTTGGGGACGGGTTAGCAGCCGGCGGGGTCGgggcgcca                                                                                                                                                                                              >  1:3018/1‑224 (MQ=255)
                            caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCCGCCCA‑GGCCCTCCTGGAGAGGGCGCAGCAGCAGGCCGTGTCTGCgccgca                                                                                                                                                                                              >  2:657055/1‑228 (MQ=255)
                               gATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGGTGAGGACCACTTTGCGGCGGCGTA‑GCCCCTTGTGGGGAGCGGTTAGCCGCAGGCTTGGGCGGCGCCGCAgg                                                                                                                                                                                            >  1:400019/1‑227 (MQ=255)
                                                   gCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCCGCGGCCTA‑GCCCCTTGTTGGGAGCGGGTAGCAGACAGCTGTGTCGGGCCAGCCGGCGCCGTAGC‑CGCATTTcc                                                                                                                                                                         >  1:497150/1‑225 (MQ=255)
                                                                       tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCCCCTTTAGGCGCCGGA‑GGCCCTTGTGGGGGGCGGGTAGCACCAGGCCGTGTCGGCGCAGCAGGCGCCGGAGCGGGCTTTTCCTG‑CGCCCGGTGcggcgggggc                                                                                                                                                   >  2:158895/1‑223 (MQ=255)
                                                                         cGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGc                                                                                                                                                <  1:31004/228‑1 (MQ=255)
                                                                                                   gcgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAGTGCCCCCCCGGCGCACGCGCAGGCGGTTGAGCTCCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAgg                                                                                                                      <  1:470593/228‑1 (MQ=255)
                                                                                                    cgcAAGGGGGAAAGAGAGCCAGATTCCCCGGCGTCCCTGGCTACGATAGCCCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGt                                                                                                                     <  2:3018/228‑1 (MQ=255)
                                                                                                                        ccaTTCCCCCGCGTCCCCGGGTCCGATAGCTCCCCACTATTGCCCCCCCGGCGCCCGGGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGt                                                                                                  <  2:79321/225‑1 (MQ=255)
                                                                                                                        aGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGt                                                                                                  <  1:158895/227‑1 (MQ=255)
                                                                                                                             ccccGGCGCCCCTGGCTGCGATAGCTCGCCATTATTGGCCGCCCGGGGCACGCGCAGGCGGTTGAGCCCCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCg                                                                                            <  2:577729/227‑1 (MQ=255)
                                                                                                                              cccAGCGTCCATGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCg                                                                                            <  1:494912/227‑1 (MQ=255)
                                                                                                                                              tcgtgtccccGCCCCTGTCGCCCCCCCGGCGCATGCGGAGGCGGTTGGGCCCCAGTTTGCGGTGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGc                                                                           <  2:599780/219‑1 (MQ=255)
                                                                                                                                               cGATAGCTCGCCACTATTGCCCTCCCGGCGCTCGCGCAGGCGGTTGGGCACCCCTTTGGGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGc                                                                           <  1:523275/227‑1 (MQ=255)
                                                                                                                                                                       ccGGCGCACGCGGGGGCGGTTGAGCCCCCCTTTGCGGCGGGGGG‑GCCGTTTGGGGAGAGGGAGTAGCAGCAGGCTGTGGCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAg                                                  <  2:497150/228‑1 (MQ=255)
                                                                                                                                                                            gcatggtctggCGGTTGACGCCCTATTTTCGGTGGCGTA‑GCCGTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGTAGGCGCAGGAGC‑GGCATTTCCTGTCGCCGGTTGCTGCGGCGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAaca                                             <  2:124569/219‑1 (MQ=255)
                                                                                                                                                                            gcACGGGCAGGCGGTTGGGCACCACTTTGGGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGGGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAaca                                             <  1:386881/228‑1 (MQ=255)
                                                                                                                                                                                            tgccccccttttGGGGCGGCGTA‑GCCCTTTGGGGGGGGCGATTCGCAGGAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATCCCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCg                             <  2:175955/219‑1 (MQ=255)
                                                                                                                                                                                            gggctcccgtttGGGGCGGCGTA‑GGCCTTTGTGGGAGGCGCTTTGCAGCCGGCTGGTGCGGCGGGGCCGGCGCAGGTGC‑TGCAGTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCg                             <  2:235173/219‑1 (MQ=255)
                                                                                                                                                                                                    cttgggggcggccGA‑GGCCTTTGGGGAGGGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCCCAGTAGC‑TGCCTTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAt                      <  1:652501/221‑1 (MQ=255)
                                                                                                                                                                                                    atttggcgcggcgGA‑ACCCTTTGTGGGAGGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAt                      <  1:154185/220‑1 (MQ=255)
                                                                                                                                                                                                         cggcggcgTA‑GCCCTTTGTGGAGAGGGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGt                 <  1:696466/227‑1 (MQ=255)
                                                                                                                                                                                                            gggcgTG‑GCCCGTTGTGGAGAGACGTTACCAGGAGGCTGGGTCGGCGCAGCAGGCGCAGTAGC‑TTCATTTCCTTTCGACGGTTTCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAg              <  1:376678/226‑1 (MQ=255)
                                                                                                                                                                                                            cggcgTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGTAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCCATACCTTCGGCATCTTCTGCCTCAGACGTCCCTTAGCAACACGCTGCACCTCTTCCGGCGTCATCGGGtttg              >  1:106478/1‑225 (MQ=255)
                                                                                                                                                                                                              cgaggaggccTTTTGGGGGAGCGATTAGCCGGAGGCTGTGGCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGGGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAgcg            <  1:507467/220‑1 (MQ=255)
                                                                                                                                                                                                                         ttGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCCGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACCTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCCCCGttcacat  >  1:380243/1‑222 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                             
GATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGAT  >  NC_000913/3398719‑3399161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑