| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 272,636 | T→G | 20.2% | noncoding (57/75 nt) | eyeA → | novel sRNA, function unknown, CP4‑6 propahge |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 272,636 | 0 | T | G | 20.2% | 137.4 / 4.8 | 82 | noncoding (57/75 nt) | eyeA | novel sRNA, function unknown, CP4‑6 propahge |
| Reads supporting (aligned to +/- strand): ref base T (29/32); new base G (12/9); total (41/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTACTGAAAGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAAT > NC_000913/272411‑272863 | aGTACTGAAAGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTg > 1:628355/1‑227 (MQ=255) tGAAAGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCAACGTGATCTGACAGGCCGTGCAGGAGGAACTTGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATAACGCTTGCGTTGCTGACTTCTGCCAAACTGCGGCAAGCAAGGTTAAAAAGGGCGACgg > 1:252818/1‑228 (MQ=255) aaaGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGGTACGTAAGCACTGCCGGGCGAAGGGGGTTTGAC‑AAGTATGGCAAACACCGGGGCACCGACGGCGATCACGCTTACCTTGCTGGCCTTTGCCAGATTTGGGCAACCAAGGGTAAAGAGGGCGACGGGc > 2:261956/1‑228 (MQ=255) aaaGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGGTGGACGGGc > 1:260523/1‑228 (MQ=255) aaaGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCAATGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTTCCAACTTGCGGCAAGCAGGGATAAAGAGTGGGACGGGc > 1:614666/1‑228 (MQ=255) aaaGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCATACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGCGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGAC‑ATTTATGCGAAACACCGGGGACGCAGCGTCTAGCCCGCTTGCGTTGCCGCTTTTGGCCAACTCGCGGCAAGCCAGGAAAAAAAGGGCGACGGGc > 2:619323/1‑228 (MQ=255) gttgtaagaaTATGACTCCT‑TTACTCCGCCCCTGT‑GGGGTGAAAACGTCCCCAGGGCTTACGTGATCTGACAGGCCGTGCATGAGGAACTGGCAGTGATACGGAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGc < 2:90495/223‑1 (MQ=255) ggTTGAAGAACATGGACCCGTTTACTCCCCGTCTG‑‑GGGTTGAAAACTGCCCCCCGGCCGTCGTGCTCTGACAGGCCCTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAATCACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGTAAGCAAGGATAAAGAGGTCGACGGGCat < 2:428445/227‑2 (MQ=255) ggTGGAAAAACATGAACCCT‑TTAATCCGCCGCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGGTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGc < 2:486092/228‑1 (MQ=255) ttGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGcc > 1:20709/1‑227 (MQ=255) caatcaCATGACCCCG‑TTAATCCGGGCCTGG‑GGGTTGAAAACGTCACCACGGCTTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAGGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCGGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGcctc < 2:425832/222‑1 (MQ=255) aaagaaCATGAACCCT‑TTAAGCCGCC‑GTGTGGGGTTGAAAACGCCACCAGGGCCAAAGTGATCTGTCAGTCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGcctc < 2:532578/226‑1 (MQ=255) acacacctgcaCCCG‑TTAACCCGCCTCTGT‑GGGTTGAAAACGGCGCAGCGGCCTACGTGACCTGGCAGGCCGCGCAGGAGGAACTGGAAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGcctcct < 2:682246/218‑1 (MQ=255) aagaaCATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATACAGAGGGGGACGGTCAGcctcac > 1:458557/1‑226 (MQ=255) aaacaCATGAGCCCT‑TTCATCCGCCTGTGG‑GGGTTGAAAACGTCACCAGGGCGTACGTGATCTGACAGGCCGGGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGGCGAAGGTGTTTTGAC‑ATTGATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGcctcct < 2:211273/224‑1 (MQ=255) cATGAACCCG‑TTAATCCGCCTTTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTa < 1:190421/228‑1 (MQ=255) aTGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCGGCGTCTATCACGCTTGCGTTGCTGAATTCTGCCACCTTGCGGCAAGCAAGGATCAAGAGGGGGACGGGCGGCCTCCTCAGAAt > 1:700898/1‑228 (MQ=255) aTGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAAAGGGCGACGGGCAg > 1:90495/1‑216 (MQ=255) tGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTTCGGCAAGCCAGGATAAAGAGTGGGACGGGCAGcctcccaagattg > 1:245694/1‑220 (MQ=255) aCCTG‑TTCATCCGCCTTTGG‑GGGTTGAAAACGGCCACACGGCCTGCGGGATCTGACAGGCCGGGGAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGGATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCt < 2:176661/228‑1 (MQ=255) aCCTG‑TTAACCCGGCTTTGG‑GGGTTGAAAACGTCAGCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCt < 1:608599/228‑1 (MQ=255) aCCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGCCTTCAGCCAACTTGCGGCAAGCAGGGGTAAAGATGGCGACGGCCACACTCCGCAGTATGCCt > 1:67739/1‑228 (MQ=255) ttAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGGGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTGTCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAGAAGGTGGACGGGCGGcctcc > 1:682246/1‑212 (MQ=255) ccGCCTCTGG‑GGGTTGAAAACGTCACCACGGCCTGCGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGGAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCca < 1:224987/225‑1 (MQ=255) ctctGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATTACGCTTGAGTTGATGACTTCTGCCAACTTGCGGCAAGCAAGGAGCAAGAGTGCGACGGGCAGCCCCCCCAGCATGCCTGAGACcaggaagga > 2:548252/1‑227 (MQ=255) ctctGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGCCTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGGGCCGGGCAGCCTCCTCCGACAGCCGGAGTCcaggcagga > 2:341806/1‑227 (MQ=255) ctctGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGGCCGACGGGCAGCCTCCTCAGTATGCCTGAGTCcaggcaggg > 1:303623/1‑227 (MQ=255) ctGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAAATTGCGGCAAGCAAGGATAAAGGGTGGGACGGGCGGCCTCCCCAGTATGCCTAGGTCCAGGAAGGTa > 1:203163/1‑227 (MQ=255) aaaaCGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGGCGGGCAGCCTCCTCAGTATGCCGGAGTCCAGGCAGGGAAAACGGGGAAg > 2:387503/1‑228 (MQ=255) caccacGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCg > 1:693045/1‑228 (MQ=255) caccacGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTAAGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCg > 1:489210/1‑228 (MQ=255) caccacGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCAGGTCGAAGGCGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGAATCCCTGAGCCGCGGGAGGTAAACCGGGCAGGGtacggg > 1:672964/1‑223 (MQ=255) ccacGGCCTTCGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGa < 1:483010/227‑1 (MQ=255) cccggtcaatgTGAGCTGGCGGGCCGTGCGGGTGGAACTGGCAGTGATACGTAAGCACTGCGGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATGACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCGCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg < 2:252818/218‑1 (MQ=255) gTGATCTGACCGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAAc < 1:279701/200‑1 (MQ=255) gTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAgg < 1:341806/228‑1 (MQ=255) cGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCAAGGCAGGTAAACCGGGGAGGGCCAGCGACGGATGTTAAGGGGGCATGGCTcgc > 1:311795/1‑225 (MQ=255) tGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGGGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCAGGGCTCCg > 1:539225/1‑225 (MQ=255) tGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCg > 1:26626/1‑225 (MQ=255) gCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg < 1:583965/201‑1 (MQ=255) ggaACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGGGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGGCAACCGGGGGAGGTCAGCGACGGGGGTTAAGGGGGCCTGGCCCCACTGAcgccgg > 2:263198/1‑225 (MQ=255) aCTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGGGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGCCGGATGTTAAGGGGGCATGGGTCCGTTGACCCcctgg > 1:291934/1‑227 (MQ=255) tGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAg > 1:134748/1‑228 (MQ=255) gacaagTAGGCACTGCCGGTCGAAGGTGTTTTGAC‑CTGTAGGCGAAACGCCGGGGCAGCAGCGTCGATCACGCTGGGGTTGCTGGCTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc < 2:693045/223‑1 (MQ=255) gATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGGAAACCGGGGAAGGTCAGCCGCGGGTGTTAAGGGGGCTTGGGTCCGATGAAGCGCTGGcggacggaag > 2:131391/1‑226 (MQ=255) atcccTGCAGGGCGAAGGTGTTTTGAC‑ATGTATGCGAAACTCCGGGGCAGCAGCGTATAGCACGCTTGTGTTGCTGACGTCTGCCAACGTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAACCCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCgta < 1:627709/223‑1 (MQ=255) tGCGGGTGGCAGGTGTTTTGCC‑AGTTATGCGAACACCCGGGGCAGCAGCGTCTATCACGCTGGCGTGGCTGGCTTCGGCCACCTTGCGGCAAGCAAGGATAAAGGGGGCGACGGGCAGCCTCCTCAGTATGCCTGAGGCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTagtagt < 2:188030/227‑1 (MQ=255) tGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGa > 1:30536/1‑172 (MQ=255) tgcggggtaaGGTGTTTTGAC‑ATGTATGGGAAACCCGGGGGCAGAAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCACCTTGCGGCAAGCAAGGATAAAGAGGGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTcc < 2:203163/220‑1 (MQ=255) gCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGa > 1:510330/1‑167 (MQ=255) gCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAt < 2:30536/172‑1 (MQ=255) ccGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGGCAGCGAc < 2:510330/167‑1 (MQ=255) tcggagggtTTTTACAATGTATGCGAAACTCCGGGGCAGCAGAGGCTATCACGCTTGCGTTGCTGCCTTCTGCCAACTGGCGGCAAGCAAGGATAAAGAGGGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGAt < 2:458557/221‑1 (MQ=255) ggTTTTTGGAC‑ATTTATGCGAAACGCCGGGGCAGCCGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 1:459846/228‑1 (MQ=255) ggTGTTTTGTC‑ATTTATGCGAAACCGCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGGGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 1:141966/228‑1 (MQ=255) tGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGGCCGCGATGGGGAAAGCCCATTACa > 1:626427/1‑228 (MQ=255) tGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGGATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGACAGCGACGGATGTTAAGGGGGCATGGTTCCGAGGACGCGCTGGCTGGCGGGGCTTCGGAAGTAGTCCGCGATGGGGAAAGCCCATTaaa > 1:425747/1‑226 (MQ=255) tATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGCCGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGCCGCGCTGGCTGGCTGAGCCTCCTGAGAAGTCCCCGACGGGTAAAGCCCATTACagg > 2:448689/1‑226 (MQ=255) aaCACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGCAAACCGGGGAAGGTCAGCCAGGGATGTTAAGGGGGCATGGCTCCGATGAGGCGCTGGCTGGCGGAGCGTCCGGCGAAGTCCGCGAGGGGGAAAGCCCATTaaagggggaggg > 2:698644/1‑217 (MQ=255) ggCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGc > 2:370012/1‑76 (MQ=255) gcagcaGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCa < 1:370012/76‑1 (MQ=255) gcagcGTCTAACACGATTGCGTTGGCGGCTTCGCGCAATTTGGGGGAAGGAAGGGTAAAGAGTGCGACGGGCAGCCTCCCCAGTATGCCGGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGATGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGa < 2:291934/228‑1 (MQ=255) cagcGTCCATCACGCTTGCTTGGCTGACTCGTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGaa < 1:448689/228‑1 (MQ=255) tCACGCTTGCGTTGCTGGCTTCTGCCAACTTGCGGCAAGCAAGGATAAAGGGTGCGACGGGCAGCCTCCGCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTg < 2:628355/227‑1 (MQ=255) gctttGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGa < 1:548252/224‑1 (MQ=255) cTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAg > 1:248052/1‑146 (MQ=255) cTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTaa < 1:57996/226‑1 (MQ=255) tgccgtCTGCCAACTTGCGGCAAGGAAGGATAAAGGGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGGTGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTg < 1:263198/223‑1 (MQ=255) tGACTTCTGGCAACTTGCGGCAAGGAAGGATAAAGATTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGGCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc < 2:248052/146‑1 (MQ=255) aCTTCCGCCAATTGGCGGAAAGCAAGGATAAAGGGGGCGGCGGGCAGCCTCCTGAGTATGCCGGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGAc < 2:78519/228‑1 (MQ=255) cTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGAc < 1:387503/227‑1 (MQ=255) ttCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGGGAAGGGAAGCAGTTTGAATGTGTTTGAGACGTGGATTAACTgg > 1:78519/1‑224 (MQ=255) tGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGCCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGAAGCGCTGGCTGGCGGAGCTTCCGGAGTAGGCCGGGCGGGGGGAAGCCCCTTCCATGGGGAAGGGAACCAGGTTGAAAGTGTGTTGGACGGCAATTAAATAACCaaaa > 2:671903/1‑227 (MQ=255) gccagtttGCGGCAGGCAAGGATAAAGGGTGCGGGGGTCAGCCTCGTCATTAGGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATTTTAAGGGTGCATGGCTCCGATGACGCTCTGGTTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGTCCTAACg < 2:61155/222‑1 (MQ=255) gCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACTCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAAc < 1:529420/227‑1 (MQ=255) gCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACg < 1:662614/228‑1 (MQ=255) ttGCGGGAATGAAGAATAAAGGGGGGGGCGGGCAGGCTCCTGAGTATGGCTGAGGGCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCGCCGATGACGCGCTGGATGGCGGAGCTGCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGa < 2:134748/228‑1 (MQ=255) tGCGGGAAGGAAGGATAAAGGTGGCGGAGGGCAGCCCCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGaa < 2:489210/228‑1 (MQ=255) tgggCCAGCAGTGATTAAGATGGCGACGGGCAGCCTCGACAGTATGGCTGGGGCCGGGCAGGTAAACCGGGGAAGGTCAGGGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGGTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGaa < 2:67739/225‑1 (MQ=255) caagGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGCCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGGAGTCCGGGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCGGTTTGAATGGGTTTGCGACGGGAATTAACGTACCTAACAAGGGGAAAACCTTTGa > 2:473464/1‑228 (MQ=255) gATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGAc > 1:573966/1‑188 (MQ=255) agagTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGGAGTAGTCCGCGATGGGGCAAGCCCATTACATGGCGAAGGGAAACAGTTTGAATGTGTTTGCGCCGGGAATTAACTGACCTACAGAGGGGGAGACATTTGTAAATCAGCg > 2:346076/1‑228 (MQ=255) tGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGCCGCGCTGGCTGGCGGGGCGTCCGTGGTGGATCGCGCAGGGGAAACACCATTACCTGGCGAAAGGGAGCAGTTGGCATTTGTTTGCAGAGGGAATTAACTGACCTCCCGGGGTGCAGACCTTTGATAATAAGCGcaaa > 2:534214/1‑227 (MQ=255) tGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGATTGAATGGGTTTGCGACGGGAATTAACTGACCTAACGAGGGGAAGACCTTTGATAATCAGCGaa > 1:377943/1‑226 (MQ=255) | AGTACTGAAAGGTTGAAGAACATGAACCCG‑TTAATCCGCCTCTGT‑GGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAC‑ATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAAT > NC_000913/272411‑272863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |