| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 404,848 | A→G | 14.9% | L266P (CTC→CCC) | proC ← | pyrroline‑5‑carboxylate reductase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 404,848 | 0 | A | G | 14.9% | 167.5 / 4.7 | 79 | L266P (CTC→CCC) | proC | pyrroline‑5‑carboxylate reductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (25/40); new base G (11/2); total (37/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCA > NC_000913/404624‑405061 | gCCATGTTACGGGTTCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAg < 1:220788/226‑1 (MQ=255) aTGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGGGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAACGGGCGGCCGC‑AGGTCCGGCGA‑AAGTCGACAGGGTTGTCTGGGgtttt > 1:675303/1‑228 (MQ=255) cGGTTGAATTAAGGGCAAAATTAGTTCCGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGGCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGAtt < 2:189783/228‑1 (MQ=255) cGGGTGCATTAAGGGCTAAATACATTACGTTTGCGGAATGCGCCACAGTTAACTTTGCGGATTAGTGTGGGGGTTGCGTCGCTGAACCCACAAATGAGTCACTAGCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGAtt < 2:540951/228‑1 (MQ=255) gCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AGGACATACGGAGTTTCTGGGGTTTTCTGATTTTTc > 1:326376/1‑227 (MQ=255) gaTGAAGGGTTAAATACATTACGTTTGCCGAATAACCCAAAGGTAACTTTACGGATTAGGTTGGGGGTTGCGCCGCTGAACCCAAAAATGAGGCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCa < 1:580631/227‑1 (MQ=255) cttGAAGTGTTAAATTCATTAGGTTTGCCGACTACGCCAACGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTAGCGGGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCa < 2:298886/226‑1 (MQ=255) cATGAAGGGCTAAATTCATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCa < 1:141006/228‑1 (MQ=255) cATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCCGCTC‑AAGTCATCAGGGTTTGCTGGGTTTTTCTGATTTTTCCa > 1:631873/1‑228 (MQ=255) cATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCCG‑ACGACACGCGA‑AAGACCACAAGAGTTGCTTGGGTTTTCTGTTTTTTCCa > 2:32509/1‑228 (MQ=255) aaTGAAGGGCTAAATACATTGCGTTTGCCGAATGCGCCACAGGGAATGTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCGTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCa < 2:530188/227‑1 (MQ=255) ggCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑CCGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGGTTTTCTGATTTTTCCATaacat > 1:86097/1‑223 (MQ=255) aTTACGTTTGCCGAATATGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGGGTTTTTCTGATTTTTCCATTAACTTCGTCATCGCt > 1:147519/1‑228 (MQ=255) aTTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTTTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCt < 1:178569/228‑1 (MQ=255) tgcGTTTGCCGAAATCGCCACAGGTAACTGTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCAAAAATGAGTCACTATCGTGAGTGGTTGAAATCGGAAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCt < 2:86097/224‑1 (MQ=255) tACGTTTGTCCAATCCGCCACATGTAACGTTACGGATTAGTGTGGGGGTTGCGCCCCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCTCCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCt < 1:290418/226‑1 (MQ=255) tACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTTGCGGCCTG‑ACGTCCGGCGA‑AAGACATCAGGATTTGCTAAGTTATTCTGCTTTTTCCCTACCCTTCGTAATCGCtc > 1:94490/1‑226 (MQ=255) tACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTT‑ACGACCGGCGA‑AAGTCATCAGGCTTTGCTGGGTTTTTTTGATTTTTCCTGAAACTTCGTCATCCCtt > 1:637776/1‑227 (MQ=255) gTTTGACGAAAACGCCACAGGTAACTTTCCGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGTGCTTTACAACGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATTCACTTCGTCATCGCTTCGAt < 1:173184/228‑1 (MQ=255) ttGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAACTGAGTAACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGG‑AAGTCATCAGGATTTGCTTAGTTTTTCTGATTTTTCCATCAACCTCGTAATCGCTTCGTTCa > 1:79714/1‑228 (MQ=255) tGCCGAATACGCCACCGGTAATTTTTCGGATTAGTTGGGGGTTTTGGCCGGTGAACCCAAAAATGAGGCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCAc < 2:79714/228‑1 (MQ=255) tGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCCG‑ACGTGCGGGGA‑AAGACATAAGGATTTGCGTAGGTTTTCGTGTTTTTCCCTAACATTCGCCAACGCTTCGATAAc > 2:178569/1‑228 (MQ=255) ccGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTTATGAGGTTTTCTGATTTTTCCATAAACTTCCTCATGCCTTCGATTACt > 1:397617/1‑227 (MQ=255) cacaGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATAAAATTCGTCATCGCTTCGCACACTGCC‑G‑CACGGa > 1:564140/1‑227 (MQ=255) taagtgtgcGGATTAGTGTGGGGGTGGCGCAGGTGAACCCACAAAGGAGTCACTATCGTGAGTGGTTGAAATCGGCAGAGTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCttt < 1:629149/220‑1 (MQ=255) cGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCCTCAGGATTTGCTGAGTTTTTCTGATTTTTCCAAACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTcc > 1:384053/1‑227 (MQ=255) cGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCCCTTCGTTAACTGGA‑G‑CACGGAAGCCTTTCTCTTcc > 1:30975/1‑227 (MQ=255) ggATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGTCGTAAACGCACCGAAGTGGCGGCCTGCACG‑CCGGGGACAAGAACACA‑GATTTGCTAGGTTTTTCTGATTTTTCCATACCATTCGTCATACCGTCGTTCCCT‑CA‑GCCACGGAATCCTTTTTCTTCCa > 2:660281/1‑227 (MQ=255) ggATAATTGTGGGGGTTGCGCCGATGAACGCACAAATTAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGAt < 1:373538/198‑1 (MQ=255) tAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGGGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGGTCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTAcc > 1:359598/1‑227 (MQ=255) tgtgGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAACGCAAAGAAAGCCGGGCGTACCCGCACCGAAGTGCCGGCCTG‑CCGTCCGGCGA‑AAGTCATCCGGCTTTGCTGCGTTTTTCTGATCTTTTCACACACTTCGCCCTAGCCTTGACCCCTGCA‑C‑CACGCGAGCCCTTCTCTTCCCGTTcg > 2:116597/1‑225 (MQ=255) tgGGGTTTGCACCGCTGAACCCAAAAATGTGCAACCAGCGTGAAGGGGTGAACTCGGCCGAGTTGGTGCCTTACAATGCAAAGAAAACCGGACGTAACCGCGACGAATGGGCGTCCTG‑GCGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTTATTTTTCCATACCCTTCGTCATCTCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTCCGCGTAc < 2:675303/228‑1 (MQ=255) tgGGGTTTGACCCGCTGAACCCAAAAATGATTCCATATCGTGAGTGGTTGAAATCGGCAGTTTTGGGGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTT‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACCCTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTAc < 2:637776/228‑1 (MQ=255) tgGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTAc < 1:471218/228‑1 (MQ=255) gggTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGGCCGGCGA‑AAGTCATCAGGATTTTCGGAGGTTTTCTGATTTTTCCATACACTTCGTCCTCCCCTTCATTACTTCG‑C‑CACGGAAACCTTTTCCTTCCAGTAccctgccccc > 2:524250/1‑219 (MQ=255) gcgcCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAATAACGCCGGACGTAACCGCACCGAAGTGGCGGCCTC‑GAGTCCGGCGA‑ACGGCATTCGGCGTTGCTGGGGTTTGCTGGTTTTTCCATACACCTTCTCATCGCGTAGATCCATGTA‑G‑CCCCGAAGCCTTTCTCTTCCCAGACCCGGACAGCTTCa > 2:362755/1‑227 (MQ=255) gAACCCACAAGTGGGTCCCTGTCGTGAGTGGTTGAAATCGGCAGATTTGGCCCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATggtggt < 1:281896/227‑1 (MQ=255) tGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGGGGTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCC‑G‑CACGGAAGCCTTTCTCTTCTACTACGCGTACCGCTTCAATGCGGGTGCCTCCCGGTGa > 1:596971/1‑228 (MQ=255) aGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGc < 1:440858/228‑1 (MQ=255) aGTCACTATCGTGAGTGGTTGAAAGCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGGAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAg < 1:58653/227‑1 (MQ=255) gTCACTATCGCGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGG‑AAGGCATCCGGAATTGCTGAGTCTTTCTGGTTTTTCCATCCACTTCCTCCTCGCTTCGCTCCCTCCACC‑CGCGGAACCCTTTCT‑TTCCAGGACCCCTACCGCTTCAATTGTGGGGTCTCCCggggcgc > 2:9840/1‑222 (MQ=255) tCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCCTCCGGGTTTGCTGAGTTTTTCTGATTTTTCCATACACCTCGTCATACCTTCGATCACTGCA‑G‑CACGGAATCCTTTTTCTTCCAGTAACCCTACCCCTTCAAATGTGGTGTCTCCCCGTggataa > 2:440858/1‑223 (MQ=255) tccTATCGTTAGTGGTTGAAAGCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACCTAACCGCACCGAAGTGGCTGCCTG‑ACGGCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGa < 2:385098/226‑1 (MQ=255) cTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGGCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACCCGTACCGCTTCTATGGGGGGGGCCCCCGGTggagcgacc > 2:93163/1‑220 (MQ=255) tagcGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCa < 1:93163/220‑1 (MQ=255) ttGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGGGA‑AAGTTATCAGGATTTGCTGAGGTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑T‑CACGGTAACCTTTCTCTTCCATGACCAGTACCCCGTCACGTGTGGTGCCTCCCCGGGAGCAGACCACATCTTTCAGTg > 1:218626/1‑228 (MQ=255) ttGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGGGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGACTTTTTTTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCCCCCGGTGAGCAGTCCATATCTTTTAGTg > 1:46430/1‑228 (MQ=255) gtGAAATCGGGTGTTTTGGCCATTGCAAAAGCAAAGAACGCCGGACGTAACCGCGCCGAAGTTGCGGCCTG‑ACGTCCGGCGA‑AAGTCAGCAGGATTTGCTGAGTTTTTCTGATTTTTCCATCCACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTg < 2:631873/227‑1 (MQ=255) gggaGATTTGGGCCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCCA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCgg < 2:147519/224‑1 (MQ=255) ggCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGG‑AAGTCATCCGGGTTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGGTCCCTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCCCTTCAATGGTGGTGCCTCCCGGGGAGCAGACCCACTCTTTCAGTGCCCCCggc > 1:398563/1‑227 (MQ=255) gCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGGCCGGCGA‑AAGTCATCAGGATTTGCGGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTAGATCACTGCA‑G‑CACGCAAGTCTTTCTTTTCCCTACCCCTTCCCCCTTCACTAGTGGTGCCCCCCCGTCCTCCAACCATATCTTTCACTGCCCCCggtt > 2:676086/1‑226 (MQ=255) cAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGGCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTTCC‑G‑CACGGAAGCCTTTCTCTTCCCGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGGGAGCGGACCACATCTTTCATGGCCCCCGGaa > 1:93548/1‑226 (MQ=255) cAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTACGGCGA‑AAGTAATCAAGATTTGCTGAGGTTTTCTGATTTTTCCCTACACTTCGTCATCGCTTCGGTAACTTCA‑C‑CCCCGTAGCCCTTCTCTTCCAGATCCCGGTACGCTTCAATGTGGGGGCCTCCCCGTGAGCCGACCCTCACTTTCCCTGCCCCCCGaac > 2:690177/1‑226 (MQ=255) aGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCGG‑TCGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATg < 1:9840/227‑1 (MQ=255) aagcaaagAAAGCCGGACGTAACCGCACCTAAGTGGCGGCCCG‑AGGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTGTCCATACACTTCGTCATCGCGTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCAc < 1:406528/228‑1 (MQ=255) agaaagCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGGTTTGCTGAGTTTTTCTGATTTTTCCATAAACTTCGTCATCGCTTCGATCACTTCA‑C‑CACGGAAGCCTTTTTCTTCCACTACCCGTCCCGCTTCAATGGCGGGGGCTCCCGGTGAGTAGCCCATATCTTTCCGTGCCCCCGCGGGTCTTCCCCTTTCCACCCCCCttt > 2:629041/1‑227 (MQ=255) agaaagCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATAAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAATCCTTTTTCTTCCAGTACGCGTACCGCTTCAATGGGGGTGACTCCCGGTGAGCAGGCCCTATCTTTCCGTTACCCCGGATGTTTTCCCGTTTCCAGCAcccattt > 1:294238/1‑223 (MQ=255) agcccgccttAACCCCCCCGGAGTTGCGGCCGT‑GCTTCCGGCGA‑AAGTCATCAGGTTTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCg < 2:93548/219‑1 (MQ=255) agCCGGGCGTTACCTGCCCGGAGGTGCGGCCTT‑GCGTCCGTCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTTATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCG‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCg < 2:46430/228‑1 (MQ=255) agCCGGGAGTAACCGCACCGAGGTGGCGGCCCT‑TCGTCGCGCTA‑AAGCCATCAGGATTTGCCGAGTTTGTCTGATTTTTCCATACACTGCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGTGTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCg < 2:94102/228‑1 (MQ=255) agCCGGACGTAACCGCACCGAAGTGGCTGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCg < 1:1972/228‑1 (MQ=255) agCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGGCA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTCCCATACCCTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCg < 2:398563/228‑1 (MQ=255) agCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCg < 1:629041/228‑1 (MQ=255) gCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCCATGCCCCCGGATGTGCTCCCGTTTCCAGCACCCTTTTTTCg > 1:459147/1‑227 (MQ=255) ccGGGCGTAACCGCACCGAAGTGGCGGCCTT‑ACGTCCGGCGA‑AAGTCACCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGa < 2:359598/228‑1 (MQ=255) ccGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAg < 2:282173/169‑1 (MQ=255) ccGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACCCGTACCGCTTCAATGGTGGGGCCTCCCGGTGAg > 1:282173/1‑169 (MQ=255) cGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCCGATGTTCTCCCGTTTCCAGCCCCATTTTTGc > 1:94102/1‑224 (MQ=255) ggACGTAACCGTATCGAAGTGTCCGCCTG‑ACCTTCTGCCA‑AAGTCACCAGGATTTGCTGGTTTTTTCTGATTTTTCCATCCACTTCGGCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAAc < 2:185837/228‑1 (MQ=255) gtcgctaCCCCACCGAATTTGTGGCCTG‑ACGTCCGGCGA‑AAGGCACCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACCCTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAAc < 2:459147/221‑1 (MQ=255) cACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGACATCAGGATTTGCTGAGTTTTTCTGCGTTTTCCATACACTTCGTCATCGCTTCGCTCACTGCA‑G‑CCCGGAAGTCCTTCTCTTCCAGTACGCGTACCGCCTCACTGGGTGGCACTCCCGGTGAGCCGACCAAATCTTTCACGGCCCCCCGAGGTTCTCCCGCTTCCCGAACCAATTTTCCCGAAACCaacacctgt > 2:233257/1‑220 (MQ=255) aCCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCg < 1:233257/212‑1 (MQ=255) aCCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTGTTTCTGATTTTTCCATACACTTCGTCATCGCTTTGATCACTGCA‑G‑CCCGGAAGCCTTTCTCTTCCGTTACCCGTACCGCTTCCATGGCGGTGCCTCCCGGTGAGCCGACCACTACGTTCAGTGCCCCCGGAAGTTGCCCCGTTTCCCGCAACCCTTTTTCGGAAACCACTACAGccc > 2:296469/1‑227 (MQ=255) aaGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCCTTACCGCCTGAGc > 1:385766/1‑228 (MQ=255) gTGGCGCCCTG‑GCGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTGTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCgg < 1:676086/228‑1 (MQ=255) cTG‑ACGTCCGGCGA‑AAGTCATCAGGCTTTGCTGAGTTTTTCTGATTTTTCCATTCCCTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTta < 1:32509/228‑1 (MQ=255) cTG‑ACGTCCGGCGA‑AAGGCATCAGGTTTTTCTGAGTTTTTCTGATTTTTCCATACCCTTCGGCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTta < 2:596971/228‑1 (MQ=255) ttccGTCCGGCGA‑AAGTCCCTAGGATTTGCGCAGTTTTTCTGATTTTCCCTCTCCCCTCGTCATCGCTTCGATCCCTGCA‑G‑TACGGACGCCTTTCTCTTCCAGTACGCGTACCGCTGCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGGGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTtat < 2:294238/225‑1 (MQ=255) cgctgggtttGGTGGGTTTTTCTGATTTTTCCATCCCCTCCGTCAGCGCTTCGCTCCCTGCA‑G‑CCCGGAAGCCTGTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCa < 2:431936/221‑1 (MQ=255) | GCCATGTTACGGGTGCATGAAGGGCTAAATACATTACGTTTGCCGAATACGCCACAGGTAACTTTACGGATTAGTGTGGGGGTTGCGCCGCTGAACCCACAAATGAGTCACTATCGTGAGTGGTTGAAATCGGCAGATTTGGCGCTTTACAAAGCAAAGAAAGCCGGACGTAACCGCACCGAAGTGGCGGCCTG‑ACGTCCGGCGA‑AAGTCATCAGGATTTGCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCA‑G‑CACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCA > NC_000913/404624‑405061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |