Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,878,707	A→C	10.9%	T331T (ACA→ACC)	yeaP →	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,878,707	0	A	C	10.9%	195.7 / 2.3	73	T331T (ACA→ACC)	yeaP	diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (33/28);  new base C (2/9);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 4.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGA  >  NC_000913/1878493‑1878939                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          agtcccttaacaacGAGAATGCGGAATTTTCGGCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTa                                                                                                                                                                                                                  <  1:831260/231‑1 (MQ=255)              cGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAAAACCTTTTGTC‑GCGCATCCAGCGCAACATACCTGAgggg                                                                                                                                                                                                     >  2:878092/1‑237 (MQ=255)              cGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGCGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGCCATCGGAGAAGTCGCTTCTGAAC‑CAACCGATGCTGACAGTCCCCTGCATCTTGCCGATATTGCGAT‑TCTCCGGGGAAAACAACCACAACA‑GAAAACCCTTTTTGCC‑GCGATTCCCACCCTCAATTCCTAAGGCg                                                                                                                                                                                                     >  2:831260/1‑239 (MQ=255)                   aTGGGGATATTTTGTCGGTGCGAGAACGCCTTCAGCAGGAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTc                                                                                                                                                                                                <  2:602924/239‑1 (MQ=255)                      cGGATATTTCGTCGGTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTcaca                                                                                                                                                                                             <  1:507623/239‑1 (MQ=255)                         atatTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTc                                                                                                                                                                                                             >  1:175889/1‑220 (MQ=255)                               cgtcgCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCACCCAGCGCTACATTCCTGAGGCGTATTAACATCCTTTTGa                                                                                                                                                                                    >  1:342348/1‑239 (MQ=255)                                gtcgCTTCGAGAACCGTTTCAGCATCAAATTCGTGGAGAATATCCATTAGGTGATGTTGATTTGGATTATCCCGGTTCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACCCAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGAt                                                                                                                                                                                   <  1:996033/239‑1 (MQ=255)                                gtcgCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGAt                                                                                                                                                                                   <  1:365806/239‑1 (MQ=255)                                         ataaCGCATTCAGCAGCAAATCCGTGGAGAATATCACTTAGGTGATTTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATa                                                                                                                                                                           <  1:52674/236‑1 (MQ=255)                                                      cagcaAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGt                                                                                                                                                             >  1:544826/1‑239 (MQ=255)                                                         caAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGtatt                                                                                                                                                          >  1:607726/1‑239 (MQ=255)                                                                ggggAGAATATCAATTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTc                                                                                                                                                   <  1:748394/237‑1 (MQ=255)                                                                     gtatatCACTTAGGTGATGTTGATTTGTATTATCCCGGTACCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAACAACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGAATTTACATCCTTTTGATTGGGGAAAAAATGGGAATCGGTTTTATTTTTcgtgtg                                                                                                                                              >  2:988144/3‑234 (MQ=255)                                                                      aaTATCGCTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATc                                                                                                                                                                                                                          <  1:829404/162‑1 (MQ=255)                                                                        tatCCCTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTa                                                                                                                                           <  1:680927/239‑1 (MQ=255)                                                                                aGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCTGTCCTGTAA‑CAACAGATGCAGACAGTGCCCTGCATGATTCCGGTATTGCGATGTATCAAGAGAAAA‑AACACAAACA‑GAGAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGACGCGTAGTCACATCATTTTGATTGTTGATAACATCCGCATCGGTATTATTTTTCCGGTTATAATCTTcct                                                                                                                                   >  1:418786/1‑237 (MQ=255)                                                                                aGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTcct                                                                                                                                   >  1:317044/1‑237 (MQ=255)                                                                                aGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAAACCCTTTTGTC‑GCGCAGCCAGCGCTCCa                                                                                                                                                                                                                >  2:829404/1‑162 (MQ=255)                                                                                aGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATACAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCCCATCCTTTTGTTTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCttatt                                                                                                                                   >  2:390856/1‑236 (MQ=255)                                                                                  gTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAAt                                                                                                                                         <  2:201322/231‑1 (MQ=255)                                                                                  gTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAAt                                                                                                                                         >  1:201322/1‑231 (MQ=255)                                                                                  gTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTa                                                                                                                                 >  1:574161/1‑239 (MQ=255)                                                                                   ttgttttttttttTATTTTCCCGGGTCCGGTCGTGGCCTAGGAGAAGTGGATCCTGAAACCACCCGAGCCAG‑CAGGGCCCTGCATGCTGCCTATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTa                                                                                                                                 <  2:544826/229‑1 (MQ=255)                                                                                     aTGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAg                                                                                                                              >  2:224969/1‑239 (MQ=255)                                                                                          gATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACACCGGTc                                                                                                                         >  1:399529/1‑239 (MQ=255)                                                                                                      cccGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCa                                                                                                             <  1:825546/239‑1 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGCTCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGCAAA‑CACA‑AAACACGAACACACCATTTTTC‑GCGCATCAAGCGCCACATACCTGAGGAGTATTCACATCCTTTTGCTTGGTGATAACCTGCGAATCGGTATTATTTTTCCGGTTATAATCTTCATTCCAGCGGTGATATTTTTAACATGGt                                                                                                         >  2:784737/1‑239 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGCTGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCGTTCAACCGGTCGTTTTTTTAGCATGGt                                                                                                         >  2:679133/1‑239 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTcac                                                                                                                                                                                              >  1:998811/1‑154 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTcac                                                                                                                                                                                              <  2:998811/154‑1 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGTTAACATGCGAATCGGTATTTTTTTTCCGGTTGTAATCTTATTTTCAGCGGTGGAATTTTTAgatgggt                                                                                                         >  2:263222/1‑233 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAAt                                                                                                                                                                 <  1:704660/183‑1 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGGATTATTTTTCCGGTTGAAATCTTCGTTACAGCGGTCGGATTTTTAACATGGt                                                                                                         >  2:901813/1‑239 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGGATTCCAACCATTTTGATTGGTGAAAACATGCGAATCGGTATTATTTTTCCGGTTTTAATCTTCTTTAAAGCGGTGGGATTTTAAGCATgtt                                                                                                         >  2:580973/1‑237 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACACTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑TCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAAt                                                                                                                                                                 >  2:704660/1‑183 (MQ=255)                                                                                                          gTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCCATGCAGACAGTGCACTGCATGCTGTCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGGC‑GCGCATCCAGAGCTACATTCCTGAGGCGCATTTACATCCTTTTGCTTGGTGACAACATGCGAATCGGGATTATTTTTCCGGTTGTCATCTTCATTATAGCGGTCGAATTTTTAGCATGGt                                                                                                         >  1:738702/1‑239 (MQ=255)                                                                                                           tGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGACCTGCATGCGGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAACCA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAAt                                                                                                                                                                 <  1:885558/182‑1 (MQ=255)                                                                                                                        atatTAGAATGCGGTCCTGAAA‑CAACCAGTGCAAACAGGGCCCGGCATGGTGCCGTTATTGCGATGTATCAGGGAAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTggcgg                                                                                            <  2:317044/238‑1 (MQ=255)                                                                                                                           gtagAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCg                                                                                                                            >  2:834864/1‑203 (MQ=255)                                                                                                                           gtagAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCg                                                                                                                            <  1:834864/203‑1 (MQ=255)                                                                                                                             agAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTAtgc                                                                                      <  1:224969/239‑1 (MQ=255)                                                                                                                                    gATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTCACGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCttattgtccccg                                                                               >  2:571296/1‑228 (MQ=255)                                                                                                                                      tCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGACTCGGTATTATTTTTCCGGTTGTAATCTTCATTTCAGCGGTCTTATTTTTAGCATGGTTTTTTTTTGGCGGGTTAGCCGCCCCggg                                                                             >  2:14494/1‑239 (MQ=255)                                                                                                                                      tCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCAt                                                                                                                                   >  1:186982/1‑185 (MQ=255)                                                                                                                                      tCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTAATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGTCGGCTTTTCTTCCCCtgg                                                                             >  1:998848/1‑239 (MQ=255)                                                                                                                                      tCCTGAAA‑CAACCGATGAAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCAt                                                                                                                                   <  2:186982/185‑1 (MQ=255)                                                                                                                                                 acCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAACCACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTc                                                                                                                         <  1:561338/185‑1 (MQ=255)                                                                                                                                                           aaagggCCCTGCATGGTGCCGATTTTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTTATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAaac                                                         <  1:263222/234‑1 (MQ=255)                                                                                                                                                           aCAGTTCCCTTGATGCTGCCGATATTGCGATTTATCCGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTTCATTCCTGAGGCCTATTCACATCCTTTTGATTTGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAaac                                                         <  1:878092/239‑1 (MQ=255)                                                                                                                                                             aGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAaacaa                                                       <  1:1000688/239‑1 (MQ=255)                                                                                                                                                              tggCCCTGACTTCTGACGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACCCCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAaacaac                                                      <  2:607726/237‑1 (MQ=255)                                                                                                                                                                gCCCTGCATGCTGCCGATTTTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACCCCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCAGTGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGa                                                    <  1:988144/239‑1 (MQ=255)                                                                                                                                                                 cccTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACCCCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGa                                                    <  1:937511/238‑1 (MQ=255)                                                                                                                                                                  ccTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGatt                                                  <  2:574161/239‑1 (MQ=255)                                                                                                                                                                     gCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCCTTACAGCGGGCGCATTTTTAGCATGGTTTTTTATTTGCGGCTATTCGTCCCCCGGGGCCTAAAGAAGaaaaaaaaaaaaaaaatt                                               >  2:102949/1‑231 (MQ=255)                                                                                                                                                                          ctgcCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACCCCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTatgat                                          <  1:464980/239‑1 (MQ=255)                                                                                                                                                                           tgcCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAAt                                                                                                                                                                 <  1:116970/119‑1 (MQ=255)                                                                                                                                                                           tgcCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAAt                                                                                                                                                                 >  2:116970/1‑119 (MQ=255)                                                                                                                                                                              cctttttGCGATGTAACAGGAAAAAA‑AACACAAACA‑GAAAACCCCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGgt                                      <  2:858223/234‑1 (MQ=255)                                                                                                                                                                                atatTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGa                                                                                                                                                                                    <  1:392043/95‑1 (MQ=255)                                                                                                                                                                                atatTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGa                                                                                                                                                                                    >  2:392043/1‑95 (MQ=255)                                                                                                                                                                                atatTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCCGGGGCTTAAAAATGTAAAAAAAAAAAATATTTATAATggggtt                                    >  1:858223/1‑236 (MQ=255)                                                                                                                                                                                       gATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTA‑TATTTTTCCGGTTGTAAt                                                                                                                                         <  1:531009/130‑1 (MQ=255)                                                                                                                                                                                       gATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTA‑TATTTTTCCGGTTGTAAt                                                                                                                                         >  2:531009/1‑130 (MQ=255)                                                                                                                                                                                         ttttttagggaaaaa‑aaCCCAAACC‑AAAAACCCCTTTTGTCGGCGTATCAAG‑GCTTCCTTCCTGGGGCTTATTCCCATCCTTTTGATTGGTTATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGatta                            <  2:547404/227‑1 (MQ=255)                                                                                                                                                                                                  ggaAAA‑AACACAAACA‑GAAAACTCCTTTTTTC‑GCGCATCCCGGGCTACATTCCTGAGGCGTATTCACATCCTTTTGATGGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTAc                   <  2:342348/237‑1 (MQ=255)                                                                                                                                                                                                    gaca‑aaCCCAAACA‑GAAAACCCCTTTTGTC‑GGCCATCCAGCGCTACATTCCTGGGGCTTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACt                  <  2:544649/234‑1 (MQ=255)                                                                                                                                                                                                      aa‑aaCACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGGATAAAGATTGAAAAAACAACCATTATTTTGATGGGTGTTGCGGTTATTGTCGCACTccg               >  1:363128/1‑236 (MQ=255)                                                                                                                                                                                                       a‑aaCACAAACA‑GAAAACCCCTTTTGTC‑GCGCATCCAGCGCTTCATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCa             <  1:780474/239‑1 (MQ=255)                                                                                                                                                                                                           cacaAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTg          <  1:679133/239‑1 (MQ=255)                                                                                                                                                                                                                      aaaCCCCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGa  <  1:571296/237‑1 (MQ=255)                                                                                                                                                                                                                      aaaCACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGGGCATAAAAATGAAAAAAACAACGATTATTATTATGGGGGTGGCGGTTATTGTCGTACTCGGCACTGAGcccgtc  >  1:544649/1‑232 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          TTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAA‑CAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAA‑AACACAAACA‑GAAAACACCTTTTGTC‑GCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGA  >  NC_000913/1878493‑1878939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑