Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 189.4 / ‑6.7 73I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base C (34/35);  minor base G (1/1);  total (35/38)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCC  >  NC_000913/2132557‑2133016
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
tttAGGCCCCCCGAATCCCAACAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATTCGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                                   <  2:160566/239‑1 (MQ=255)
                     gcgCCGGAAGTTTACCGTTACGCTCGGAATGGAGCGTCAGCAAAGTGGGCACGGGCATCTGTTTAGTGCGGATCGGGTAGAGGGTAAACTGCTGGTATTTTTTGCTGGCGCTGTCGAATTTGTCGTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGt                                                                                                                                                                                                             <  2:60173/237‑1 (MQ=255)
                            ccttttGCCGTTACGCCCCGACAGCAGCGTCAGTAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                       <  2:365280/235‑1 (MQ=255)
                            aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTAACCGGGATTGATATCCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTGGCCg                                                                                                                                                                                                       >  1:953874/1‑238 (MQ=255)
                            aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGGGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCCCTTCTACTTTCCACGGttgcccg                                                                                                                                                                                                       >  1:961518/1‑233 (MQ=255)
                            aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGAACCCGGAGAATTTTTTCGGTGACGGGGTGATATGCATTTCACGGTGATTGCTAGTCAAGCCTGGCGTCACCTCTTCAACTTTCCACGGTTGCCCGt                                                                                                                                                                                                      >  2:67766/1‑239 (MQ=255)
                             cGTTTGCCGTTACGCTCCGACAGCAGCGTTAGCAAAGTGCGCCAGGGCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                                                                                                                    <  1:841153/239‑1 (MQ=255)
                                ttGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                                                                                                                                 <  1:670643/239‑1 (MQ=255)
                                ttGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGACATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCAGATATTTCTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAATTGGACGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTACCCGTGATTTGTATGCACGGTCTGCGACGCCCCTTCTCCTTTCCACGGTTCGCCGTACTc                                                                                                                                                                                                 >  2:596412/1‑239 (MQ=255)
                                 tGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGCAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                                                                                                                                 >  1:739543/1‑238 (MQ=255)
                                     gTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                                                                                                                            <  1:97322/239‑1 (MQ=255)
                                            acggccagcggGGTTAGAAAAGTGCGCAAGGTCATACGTTTATTGGGGATCTGGTAGAGGGTAAACAGCTGATTTTTTTTGCTGGCGCGGTCTAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCg                                                                                                                                                                                     <  2:943241/234‑1 (MQ=255)
                                              cGACAGCAGCGTCAGCAAAGTGCGCCAGGACATACGTTTCTTGCGGAACTGGCAGAGCGTACACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCGTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACAAGGCGATTTGCATCTAACCGTGATTGATATGCAGGCTCTCGCTCTCCTCTTCTACTTTACACGGTTCGCCGAACCCACCCACCATccccc                                                                                                                                                                                   >  2:494198/1‑236 (MQ=255)
                                                aCAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGGGGTGATCTGCATTTAACCGGATTTGATATGCAGGCTTGGCTGCGCTCCTTCTACTTTCCACGGTTCGCCGTACTccaccccccaccccggg                                                                                                                                                                                 >  2:561024/1‑230 (MQ=255)
                                                aCAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCCCTTCTAATTTCCCCGGTTCCCCGACCCCCCCCAACACCGGCAgg                                                                                                                                                                                 >  2:279479/1‑239 (MQ=255)
                                                aCAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGCAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGGCGCCTCTGCTACTTTCCACGGTTGGCGGTACCCCACACCCACCCCCAgg                                                                                                                                                                                 >  2:845881/1‑239 (MQ=255)
                                                    cagcGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                                             >  1:52202/1‑239 (MQ=255)
                                                     agcGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGGAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                                             <  1:279479/238‑1 (MQ=255)
                                                        gTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATTTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCACCCACCATCCGCAGGAGGATGTc                                                                                                                                                                         >  1:589162/1‑239 (MQ=255)
                                                        gTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTAACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAAGGTTCGCCGTCCTCCACCACCAACCCCAGGAAGagtgc                                                                                                                                                                         >  2:23617/1‑235 (MQ=255)
                                                                           ttatttctttATTTCGTATCTGGTAGACGTTAACCAGCTGATTGTTTTTGCTGGCGCGGTCGAATTGGTCTTTGTGCTTGCGGTAAATGGTGAAGTACCCGGAGAAGTGTTTCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGGCTCTTCTACTTTCCACGGTTCGCCGTACTCCTCTACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                                                                                                                       <  2:512115/231‑1 (MQ=255)
                                                                              tACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                                                                                                                   >  1:782203/1‑239 (MQ=255)
                                                                               acttttATTGCGGTTCTGGTAGGGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCCTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                  <  1:946798/236‑1 (MQ=255)
                                                                               aCGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCCGGAAGATGTCGTAATCCCGTGCCGCtttacgtt                                                                                                                                                  >  2:254341/1‑234 (MQ=255)
                                                                               aCGTTTAGTTCGGATCTGGTAGAGGGTAAACAGCTTATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTACGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                  <  1:845881/239‑1 (MQ=255)
                                                                                 gTTTAGTGCGGATCTGGTAGGGGGTAAACAGCTGATATTTTGTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGcc                                                                                                                                                            <  1:917518/227‑1 (MQ=255)
                                                                                    tATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGAACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGGCCCTGCGTCGCCTCTTCTACTTTCTACGGTTCGCCGTACTCCACCACCATCCGCAGGAAAATGTCGTAATCCTGTGCGCCTTTCAGTTAggca                                                                                                                                             >  1:988632/1‑237 (MQ=255)
                                                                                     aTTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGACTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGCATTGATATGCAGGCTCTGCTTCGCAGCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCAACCCCAGGAAAAACACGTAATCATGTGCCGCTTCCAGTCAGGTa                                                                                                                                             >  2:470115/1‑238 (MQ=255)
                                                                                           gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGTTGTCGAAATCCCGTGCCGCTTTCGGTTcctgactctcat                                                                                                                                      >  2:917518/1‑228 (MQ=255)
                                                                                           gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                                                                                                                      >  1:382949/1‑239 (MQ=255)
                                                                                               ttgTAGGGGGTAAACAGTGTATATTTTTTGCGGGCGGGGTCGAATTTTTCTTTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                                  <  2:607052/237‑1 (MQ=255)
                                                                                                       ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                                  <  1:606883/231‑1 (MQ=255)
                                                                                                       ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                                  >  2:606883/1‑231 (MQ=255)
                                                                                                       ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                                  <  1:427372/231‑1 (MQ=255)
                                                                                                       ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                                  >  2:427372/1‑231 (MQ=255)
                                                                                                       ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGGGCCGCTTTCAGTTCGGTATCGAACAAACCCCCTTTGa                                                                                                                          >  2:820979/1‑239 (MQ=255)
                                                                                                        gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                                                                                                                            >  1:1007134/1‑157 (MQ=255)
                                                                                                        gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                                                                                                                            <  2:1007134/157‑1 (MQ=255)
                                                                                                           aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGGGCCGCTTTCAGTACAGAAACGAACAAACACACTTTGaaaa                                                                                                                       >  2:418791/1‑237 (MQ=255)
                                                                                                           aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTCCAGTTCGTTATCGAACAACCACTCTTTGAAAc                                                                                                                       >  1:308310/1‑238 (MQ=255)
                                                                                                            aCAGCTGATATTTTTTGCTGGCGCTGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                                                                                                                     <  1:561024/239‑1 (MQ=255)
                                                                                                                cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGGCACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTCCTTTCCACGGTTCGCCGTCACTCACCACCATCCCCAGGAAGTGGTCGTAATCCTGTGCCCACTTCAGTTAGGTCACGAACAAAAACCCTTTAAAAGACCAt                                                                                                                  >  2:485378/1‑238 (MQ=255)
                                                                                                                 tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGTCTCTTCTACTTTCCACGGTTCGCCGtc                                                                                                                                                                                                     >  2:808171/1‑154 (MQ=255)
                                                                                                                 tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGTCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                                                                                                                    <  1:808171/155‑1 (MQ=255)
                                                                                                                  ttttttttttGCTGGCCTGGTCGAATTTTGTTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                               <  2:981494/235‑1 (MQ=255)
                                                                                                                   atttgttttGCGTGCGCGTGCGAATTTGTCTTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGGTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                              <  1:311821/234‑1 (MQ=255)
                                                                                                                   atatTTTTTCGTGGGGCGGTTGATTTTTTCTTGGTTTTTGGGGAAAAAGCTGAAGTTCCCGGAGTATTTTTTCGGTTGCGAGGTACTCTGCATATACCCGGTGTTCATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCATGGTTCGCCGTTCTCCACCACCATCCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACCCTTTTAAACGCCATGccc                                                                                                              <  2:238498/239‑1 (MQ=255)
                                                                                                                        ttttGGTGGGGCGGTAGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTa                                                                                                        <  2:69035/239‑1 (MQ=255)
                                                                                                                        ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATACGCAGGCAGCTGTCGTAATCCTGTGCCGCTTTCAGCTCGATATCGAACAAACACCCTTTGAAAAGCCATGccccgaga                                                                                                         >  2:38621/1‑234 (MQ=255)
                                                                                                                         tttGCTGGCGCGGTCGAAGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGGACCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaa                                                                                                       <  1:596412/239‑1 (MQ=255)
                                                                                                                                    ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt                                                                                             <  1:797498/238‑1 (MQ=255)
                                                                                                                                                 tttGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGc                                                                               <  1:820979/239‑1 (MQ=255)
                                                                                                                                                 ttgttgCTTGCGTGAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGAGCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGc                                                                               <  1:429194/235‑1 (MQ=255)
                                                                                                                                                         tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                                                                                                                          >  2:650709/1‑95 (MQ=255)
                                                                                                                                                         tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                                                                                                                          <  1:650709/95‑1 (MQ=255)
                                                                                                                                                         tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                               >  2:427129/1‑200 (MQ=255)
                                                                                                                                                         tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                               <  1:427129/200‑1 (MQ=255)
                                                                                                                                                              tAAAAGGTGAAGTGCCCGGGGAATTTTGTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGGGTCGCCTCTTCTACTTTCCACGGGTCGCCGTACTCCACCACCATCCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAg                                                                  <  1:494198/239‑1 (MQ=255)
                                                                                                                                                                aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAgg                                                                 <  1:638619/238‑1 (MQ=255)
                                                                                                                                                                            cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAGACCTGGTTGCCAATGATATTGCGTTTGTCGAACAGGCGGCGGGactcag                                                    >  1:378710/1‑233 (MQ=255)
                                                                                                                                                                             ccGGAGAATTTTTTCGGTGGCGGGGTGTACTGCATTTCACCGTGATTTATTTGCAGGCTTTGGCTCCGCCCTTCTTCTTTCCCCGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACgg                                                   <  2:1006042/239‑1 (MQ=255)
                                                                                                                                                                             ccGGAGAATTTTTTCGGTGCCGGGGTGTTCTGCCTCTCGCCGTGGTTGATACGCAGGTTCTGCGTTGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTGCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACgg                                                   <  1:485378/239‑1 (MQ=255)
                                                                                                                                                                               ggggtaTTTTTGCGGGGACGGGTTGGTCGCCATTTACCCGTGATTGTATTGCAGGCTGCCCGTCGCCTCTTCGCCTTTCCACGGTTCGCCGTTCTCCACCCCCAGCCGCAGGAAGATTTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCg                                                 <  2:739543/234‑1 (MQ=255)
                                                                                                                                                                                         ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATa                                       <  1:499600/239‑1 (MQ=255)
                                                                                                                                                                                                        aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGGTAAGGACCTGGTTGCCAATGATTTTCGGTTTGTAGAAAAGGCGGCGTGAATACGGCGCTTTGGGATACAGGGGccactgta                         >  2:331730/1‑232 (MQ=255)
                                                                                                                                                                                                            gCATTTCCCCTTGACTGGTAGTCAGGCTCTGCGTCCCCTCTTCTTCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGt                    <  1:418791/239‑1 (MQ=255)
                                                                                                                                                                                                            ccctttCCCCGTGATTGATGTTCCGGATCTGCGTCGCCCTGCTCCCTGTCCACGAGTTGTCGTATCCCACCCCCTTCCGCAGGAAGTTGCCGTAAGCCTTTGCCGCTTTCCGTTCGGTATCGAACAAACCCGCTTTGAAACGTCATGCCCGTGTAAAGACCTGTTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGTATGAGGCGATTTGGGAGACAGCGGCAAGCTTGCCGGt                    <  2:665380/236‑1 (MQ=255)
                                                                                                                                                                                                                ttCACCGTGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTATGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGCACCCCCATGCCCAGGAAACAACCTGGTTGCCAATGAAATTGCCTTTGTAGAACAGGCGGCGGGGATACGGGGATTTGGAGGAAAGAGGAAACGTTGCCGGTgggg               >  2:124027/1‑239 (MQ=255)
                                                                                                                                                                                                                       tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGACCAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATaa          >  1:445351/1‑236 (MQ=255)
                                                                                                                                                                                                                       tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGGGGAGAAACGGGGATTTTGGATAAAACGGCAAAGTTGGCGGTGGGGAAAAGag        >  2:26245/1‑238 (MQ=255)
                                                                                                                                                                                                                       tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGCTTTGGGCTACAGCGGCAAGCTTGCCGGTTGGGGAATAg          >  1:664189/1‑238 (MQ=255)
                                                                                                                                                                                                                        tattgaatgtcAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACt       <  1:147495/229‑1 (MQ=255)
                                                                                                                                                                                                                             ttttGCAGGCTCTGCTTCGCCTTTTTTTCTTTCCCCGGTTCCCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGcc  <  2:445351/236‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
TTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCC  >  NC_000913/2132557‑2133016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: