| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,832,077 | G→A | 5.8% | V541M (GTG→ATG) | yicH → | putative inner membrane‑anchored periplasmic AsmA family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,832,077 | 0 | G | A | 5.8% | 193.6 / 4.2 | 72 | V541M (GTG→ATG) | yicH | putative inner membrane‑anchored periplasmic AsmA family protein |
| Reads supporting (aligned to +/- strand): ref base G (47/20); new base A (2/2); total (50/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01 | |||||||||||
CGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCA > NC_000913/3831839‑3832303 | cGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGCTATTCAGGCCAATGTCCCGTTGAAACCTCCGGTTCCCGGGCAATTGCATGCCg > 1:214312/1‑239 (MQ=255) aGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGCATTACCGTTGACTGGCGACGCCAATATTCAGCTTACCGCCAGGGGCGATATTTAGGCCAATGGCCGGTGTAAGACCACGGGTAGCGGGCAAAGGCACGCCCGGAAcgc > 2:339618/1‑239 (MQ=255) gTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAAcgcc > 1:279348/1‑239 (MQ=255) aCTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTTAAACCTACGGTTACCGGGCAATTGCATGGCGAGAACGCCGCCAagc > 2:469714/1‑238 (MQ=255) gAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATTGCCTGAATTACAGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAAGCCAAGGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGAATGACGTGAAGCCCGAAAAgcggca > 1:700572/1‑239 (MQ=255) gAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCACCAAAgaagca > 1:586596/1‑239 (MQ=255) gAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATCACGTGAACGCCGCAAAgcagca > 1:330412/1‑239 (MQ=255) tCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGCTATTCAGGCCAATGTCCCTTTGAAACCTACGGTTAGCGGGCAAATGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCaa > 1:491003/1‑238 (MQ=255) tCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGGGAACGCCGCAAACCAGCAAGTGATTCaa > 1:506475/1‑238 (MQ=255) tCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGCGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGGTATTCCGGCCAATGTCCCGTTGAAACCTACGGTTAAGGGGCCATTGCATGCCGGAAACTCGCCAAAGAAGAAAGAAACTCaaa > 2:272398/1‑239 (MQ=255) gCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGaa > 1:878420/1‑239 (MQ=255) gattttttCAGAAACGCCCAAACGTCAGACACATAACAGGTGGAATGGACGGGGTGTGCGGGTAAATATTTTGAAACAGGGGGTATGGCCTGAATTCCCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑Ca < 1:182672/234‑1 (MQ=255) tCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTCCCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACACCGCAAAACAGCAAGGGACTACAAACATAAAATCCGG‑CATCGttt > 2:637098/1‑238 (MQ=255) aaagtcaaGACAATTATCGCCCTGAAGGGCCGCGGGGGCCCGGTGAATTTTTGGCAACAGTGGGGTTGGCCTGAATTACCGTGGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGt < 1:570954/232‑1 (MQ=255) tatCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGCAGTTACATCGCCGGAg > 1:762815/1‑238 (MQ=255) atCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATCCTGG‑CATCGTTTCCACCGGGGAAGTTACATCGACGGAG‑cc > 1:59374/1‑239 (MQ=255) atCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGAGACTCAAACCATGAATGCCGG‑CATCGTTTCCAGCGGTGAAGTAACATCAAAGGAG‑cc > 2:103282/1‑239 (MQ=255) ccTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACa > 1:790291/1‑223 (MQ=255) ccTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACCCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGGGAAGTTAAATCCACGGAA‑CCGGTgg > 2:714123/1‑238 (MQ=255) gAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGATTCAAACCATGAATGCTGG‑CAACGTTTCCAGCGGTGAAGTTACATCGACGGAA‑CCGGTGCggg > 1:581675/1‑238 (MQ=255) aaTGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCACTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACACTGAATCTTGG‑CATCGTTACCCGCGGTGAAGTTAAAACGACGCGG‑CCGGGGCGGtg > 2:963971/1‑238 (MQ=255) aaTGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTa > 1:300239/1‑239 (MQ=255) aaTGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGACCTGAATTACCGTTGACTGGCGCCGGCAATATTCAGATTAACGCCAGTGACGCTATTCAGGCCAATGTCCCGCTGAACCCTACGGTTAGCGAGCAATTGCATGCAGTGAACGCCCCACACCAGCAGGTGACTCAAACCATGCAT‑CTGGCCATCGGTTCCACCGGTAAAGTAACTCCAACGAAG‑CCGATACggaa > 2:847616/1‑237 (MQ=255) cgcgGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCCAGGGAA‑CCGGTGCGG‑AAACaaac > 2:1005975/1‑236 (MQ=255) cgcgGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGAGACCCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATC‑ACCGAGACCGGTGCGGGAAAAAta > 1:506870/1‑238 (MQ=255) aaTATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAg > 1:877445/1‑185 (MQ=255) aaTATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCATGAACGCCGCAAAGCAGCAAGGGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTAAATCGAAGGAG‑CCGGTGCGGGAAAAATCAATCCCGCCggggag > 2:1005855/1‑236 (MQ=255) tatTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CCTCGTTTCCAGCGGTGAAGTTCCATCGACGGAG‑CCGGTGCGGGAAACATTAATCTCCCCGGGTACg > 1:621294/1‑238 (MQ=255) tGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCAAGCGGTGATGTGACATTGCCGGTG‑CCGGTGCGGTAAACTTCAATTGCGCCGGGTACGACTCAg > 1:295265/1‑239 (MQ=255) tGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCGGACGAAccc > 1:236207/1‑239 (MQ=255) tGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATACTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGGAAACATCGATCTCGCCGGGTACGACTCAGACGTAccc > 1:909633/1‑239 (MQ=255) tGGCCTGAATTTCCTTTGACGGGCCACGGCAATATCCAGTTTCCCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCAt < 1:502055/238‑1 (MQ=255) gCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACa > 1:694045/1‑199 (MQ=255) cTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCACCGGTGAAGTTACATCGACGGGG‑CCGGTGCGGTAAACATCAATCTCGCCCGGGACGACTCAGACGTACCCGGACTTCCAt > 1:703113/1‑239 (MQ=255) aaTTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACAt > 1:169991/1‑171 (MQ=255) aaTCACCGTGGCCGGGGGACCGAAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGACCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCa < 1:60493/238‑1 (MQ=255) ccGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCaa < 1:684485/126‑1 (MQ=255) ccGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCaa > 2:684485/1‑126 (MQ=255) cGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATa > 1:240025/1‑235 (MQ=255) gtgagcgggCGAAGGAAATTTTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTa < 1:272398/232‑1 (MQ=255) gACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCTTTCCTCCAATAAATTCCaa > 1:496975/1‑239 (MQ=255) tGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCCGGAACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGt > 1:96535/1‑239 (MQ=255) tatTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAACCATCAATATCGCCGGGTAGAAAGCAGACGAACACGCCCTTCCAACAATAGGTTTAAACGTAAATGTCAgcga > 2:50796/1‑238 (MQ=255) tttactgttcccGTCAGTGGCGATAGTCAGGCCAAGGTCCCGTTGAAACCTGCGGTTAGCGGGCAATTGCATGCCGTGAAAGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGGTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCa < 1:457958/229‑1 (MQ=255) ttcgcttcgccgccgGGGGTGATTTTCAGGCCCATGTCCCGGTGAAACCTCCGGTTACGGGGCAATTGGCTGCCGTCACCGCCGCAAAGCAGCAAGTGACCCAACCCATGAATGTTGC‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGGG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt < 2:506870/230‑1 (MQ=255) tgtaggttcccGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt < 1:963971/230‑1 (MQ=255) cTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGATGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCt > 2:1022360/1‑239 (MQ=255) aCCGCCAGTGGCGATTTTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt < 2:67334/231‑1 (MQ=255) aCCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt > 1:67334/1‑231 (MQ=255) cAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt < 1:103282/239‑1 (MQ=255) cAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt < 1:714123/239‑1 (MQ=255) gTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGCAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCCCCGGGTACGACCCAGACGTACCCGGCTTTCCTACAATAGATTACACCGAACTTTTCACGGACTTCCCTTTAGGCTTc > 2:743377/1‑238 (MQ=255) gTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCCGACGTACCCGGCATTCCATCAATTGATTACAACGTAATTGGCCGCGCATTCCCTTGTGGCTTc > 1:963647/1‑238 (MQ=255) gTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCATGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca < 1:1005855/239‑1 (MQ=255) cGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcacca < 1:1007510/238‑1 (MQ=255) cGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCATGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCg < 2:749068/136‑1 (MQ=255) cGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCATGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCg > 1:749068/1‑136 (MQ=255) gATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTTAGGCTTCaccacc > 1:288200/1‑239 (MQ=255) tccggCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATGTCATGCCGTGAACGCCGCAAAGCAGCAAGTTACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGccc < 2:470588/236‑1 (MQ=255) gtgggCCATTGTCCGGGTGAAACCGACCGTTAGCGGGCAATTGCATGCCGGTAACGCCGAAAAGCGGCAAGGGACTAAAACCGGGAATGTGTG‑GAGAGGTTACAGCGGTGAAGGTACATCGACGGGG‑CCGGTTCGGTAAACATCAATCTCGCCGGTTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGccc < 2:762815/236‑1 (MQ=255) cataTGTCCCTTTGAACCCTTCGGTTAGGGGGCAATTGGATGCCGGTGACGCCGGAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTTAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCt < 2:59374/236‑1 (MQ=255) tGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTACTTGTCACCGCCTTCCCTTGAGGCTTCACAACCACCCCCTACCCACt > 2:26719/1‑239 (MQ=255) tGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAAAATCAATCTCGCCGGGTAGGACCCAGACGTACCCGGCATTCCATAACTAGATTACAACGAAATTGTCAGCGCATTCCCCTGAGGCTTCACCACAAACCCCGGccacat > 2:860007/1‑234 (MQ=255) cccGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCTGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc < 1:50796/239‑1 (MQ=255) cGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGATGCGGTAAACATCAATCTCCCCGGGTACGACTCACGCGTACCCGGCATTCCATAAATAGATTAAAACGTAATTGCCAGCGCATCACCTAGAGGCTTCACCACCAACCCCTCCCCACGTTCa > 2:472055/1‑238 (MQ=255) ttGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc > 1:680013/1‑238 (MQ=255) aaaCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGGGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCtcc > 1:516591/1‑237 (MQ=255) ccTTCGGTTAGCGGGAAATTGCATGGCGTGAACCCCGGAAAGGAGCAAGTGACTCAAACCATGAATGCTTG‑CATCGTTTCCAGCGGTGAATTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGTATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg < 1:305477/237‑1 (MQ=255) ccTACGGTTAGGGGGCAATTGCATGCCGTGACCGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGaa < 1:469714/239‑1 (MQ=255) ccTACGGTTAGCGGGAAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGaa < 1:85775/239‑1 (MQ=255) aGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCCCTTTCAGCCTGCGAACCCGCTTGc > 1:864627/1‑239 (MQ=255) aGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCgctgtct > 1:697791/1‑233 (MQ=255) cAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTCCAACGTAATTTTCAGCGCATTCCCTTAGGCCTTCACCCCCAGACCCAGCTCACTTTCAACATGCGAACAGACTTGCAAACCa > 2:90575/1‑239 (MQ=255) | CGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGACACATATCAGCCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGG‑CATCGTTTCCAGCGGTGAAGTTACATCGACGGAG‑CCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCA > NC_000913/3831839‑3832303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |