Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,056,115 A→C 20.7% G75G (GGT→GGG glnL ← sensory histidine kinase in two‑component regulatory system with GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,056,1150AC20.7% 142.7 / 6.2 62G75G (GGT→GGGglnLsensory histidine kinase in two‑component regulatory system with GlnG
Reads supporting (aligned to +/- strand):  ref base A (45/2);  new base C (2/13);  total (47/15)
Fisher's exact test for biased strand distribution p-value = 1.23e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGT  >  NC_000913/4055881‑4056347
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
cTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATCCGAGCGCCCGTCGATGACCAGCGTCCCTTCGTTATCGGTAAAACCt                                                                                                                                                                                                                                        >  1:1017083/1‑238 (MQ=255)
     ccgcgccACGTAAACGGCCAAGCGGATTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTACCGGATCCTGACTTAAGCGGCGCTGGGTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCAGCAATACGCTGGGCCGTCCCAGAAAGGTTATACAGGCGCCCGCGGCAGACCCGGGTCCCTTCGATACCGGAAAAACCtacccgc                                                                                                                                                                                                                                  >  2:903190/1‑233 (MQ=255)
                                tttttctctcCTGGGCCGCGCCGCGCACTTAACCGCGGGGAGCCACCTGTTGGGGGGGGGGTTGGTGTTCCCGAACTGAGGCGGCGTTGTTTTCCCACCGGAGCCTCCCCAGAAGGGTCCTTGCCGCCCGGATTCCGCTGGGCCGTCACAGAAAGGATATGCGCGCCCCCGTCGAGGACCAGCGTCACTTCGTTGTCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTc                                                                                                                                                                                                        <  2:1000804/233‑1 (MQ=255)
                                ttttAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGa                                                                                                                                                                                                                         >  1:918680/1‑221 (MQ=255)
                                          aTGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGAGATTTAAt                                                                                                                                                                                              >  1:1000804/1‑238 (MQ=255)
                                                    ccGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGAAGCt                                                                                                                                                                                   >  1:199528/1‑239 (MQ=255)
                                                    ccGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCCCGTATTTTAATGGGAAGTAGcc                                                                                                                                                                                   >  1:534203/1‑238 (MQ=255)
                                                       cgcACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTaaa                                                                                                                                                                                >  1:979160/1‑239 (MQ=255)
                                                       cgcACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGAAGCTCac                                                                                                                                                                                >  1:868143/1‑238 (MQ=255)
                                                              aaTCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATTCGAGCGCCAGTCGATGACAAGCGTCACTTCGTTCTCGGTAAACCCTTGCACCGCCTCCAGGCTTTCCTGCTTCAGCTCGTATTTTAATGGAAAGTAGccaaccaggtac                                                                                                                                                                         >  2:934926/1‑228 (MQ=255)
                                                                    gggCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCCCAAAAGTTCCGGTAAc                                                                                                                                                                   >  1:928321/1‑239 (MQ=255)
                                                                      gcagcaACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATCCCGTCCGGCATACGCTGGGCCGGCACAGAAAGGATATGCGAGCGCCCGTCGATGACCACCGTCACTTCGTTCTCGGTCAAACCTGGCCCCGCCTCAAGCCTTTCTTGCTTCAGCTCGATATTTAATCAGAAGGAGCCCAACAGtgcagggaaagg                                                                                                                                                                 >  2:751876/1‑228 (MQ=255)
                                                                        agcaACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAACGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTCCTTGCCTCACCTCCATCTTTAATTAACAGTATCCAAACCTTCCCGGTAActgg                                                                                                                                                                >  2:316124/1‑235 (MQ=255)
                                                                         gcaACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATAGTCGAGAGCCCGTAGATGACCATCGTAACTACGTTCTCGGGAAACCCTTGCCCCACCTCCCGACTTTCTTGCATCTCCACCATAATTAATAAAAAGTGACCCAACAGTTCCGGAAACAgtgt                                                                                                                                                              >  2:762661/1‑239 (MQ=255)
                                                                                        tGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTTCg                                                                                                                                               >  1:256158/1‑239 (MQ=255)
                                                                                                   ttCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAACACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCACCAGTTCCGGTAACGGTGTACCCAACCATTTTGGGGAGCTTTtgg                                                                                                                                    >  2:931295/1‑239 (MQ=255)
                                                                                                       tGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGCTGACCAGCGTCACTTCGTAATCGGTAAAACCTCGCCCCGCCTCCAGACTTTCTTGCATAAGCTCGATATTTAATGAGAAGTAGCGCAACAGTTCCGGTAAAGGTGAACCAAACAATTTACGGAGGCTTATGGCGa                                                                                                                                 >  2:211235/1‑238 (MQ=255)
                                                                                                              aaGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCCCAACAGTTCCGGCAACGGGTTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAgttg                                                                                                                          >  2:54711/1‑239 (MQ=255)
                                                                                                              aaGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGAAATTTATTGGGAAGTAGCCCAACATTCCCGGTAACGGGTAACCAACCAATTTGCGGGGGCTTTGGGCGGCCAgttt                                                                                                                          >  1:611810/1‑238 (MQ=255)
                                                                                                               aGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACGGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGGGGGAGCTTTGGGCGGGCGgttgt                                                                                                                         >  2:407989/1‑239 (MQ=255)
                                                                                                               aGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCCCTTCGTTATCGGTAAAACCTTGCCCCGCCTCCCGCCTTTCTTGACTCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACCGTGTACCAAACAATTTGCGGGAGCTTTGGGGcagcaggttg                                                                                                                         >  2:199076/1‑235 (MQ=255)
                                                                                                                  ggggttGGTTATCCAGCGGGCCCATCCCCAGCAGGATCATGCCGCCCGGCATACGCTGGGCCGTCAAAGAAAGGATTGGCGAGCCCCCGTCGATGCCCAGCGTCACTTCGTTACCGGAAAACCCTGGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAgttgtt                                                                                                                        <  1:962346/232‑1 (MQ=255)
                                                                                                                    cgcTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCCCTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGGGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAACCAATTTCCGGAAGCTTTGGGCGCGCAgttgttcgca                                                                                                                    >  1:707124/1‑235 (MQ=255)
                                                                                                                    cgcTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGCAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGATTTGGGGGAGCAGttgttgggg                                                                                                                    >  2:730879/1‑236 (MQ=255)
                                                                                                                      cTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGcgcg                                                                                                                   >  1:729690/1‑238 (MQ=255)
                                                                                                                            tccctgcGGGGCCATCTCCAGTCGGACCCTGCCGTCCGGAATTAGTGGGGCCGTCCAAGAAAGGATACGCGACCGCCCGTCGTCGCCAAGCGTCACTTCGTTATCGGTAACCCCTTGCCCCGCCTCCAGACTGTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGt                                                                                                            <  2:912812/233‑1 (MQ=255)
                                                                                                                            acccagggggcccATCCCCAGAGGGAACAGCCCGCCGGGCACGCGGTGGCCCGTCACAAAAGGGATAGGCGAGCGCCCGTCGATGCCCAGCGTCACTTCGTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGt                                                                                                            <  1:926469/228‑1 (MQ=255)
                                                                                                                                  cGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGCAACGGTGTACCAAACAATTTGCGGGGACTTTTGGCGGGAAGGTGTTGGGCGGCAgggggggggt                                                                                                      >  2:156882/1‑232 (MQ=255)
                                                                                                                                       ccATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGCCCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCGGTTGTTGCGCGGCAGGGTTGGCGTATTgg                                                                                                 >  1:72864/1‑239 (MQ=255)
                                                                                                                                           ctcCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAACCAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGAGCGGCAGGGTTGGCGTAATGGATc                                                                                              >  1:360301/1‑238 (MQ=255)
                                                                                                                                                         aTGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTACCGGTGTACCAAACAATTTCCGGGAGCTTTGGGCGAGCCGTTTTTTCCCGCCCGGGTTGGCGTAATGGATCGCCAGGTTGtatc                                                                                >  2:374283/1‑235 (MQ=255)
                                                                                                                                                            ccGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCCGGGTTGGCGTAATGGATCCCCAGGTTGTCATCgct                                                                            >  1:342809/1‑237 (MQ=255)
                                                                                                                                                               tCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGa                                                                                                                                                                                                                         >  1:898189/1‑96 (MQ=255)
                                                                                                                                                               tCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTTGGCGAGAAGTTGTGGCGCGGAGGGGTTGGGGTAATGGATCGCCGGGTTGTCATCGATTaa                                                                         >  2:873602/1‑239 (MQ=255)
                                                                                                                                                               tCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGAACGCCAGGGTGGCATGGaaaaa                                                                         >  2:404168/1‑235 (MQ=255)
                                                                                                                                                               tCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGCAACGGGGTACCAAACAATTTGCGGGAGCCTTGGGCGCGCAGTTGTTGAGCGGCCAGGTTGGCGTAATGGTTAGCCAGGGTCTAATCGATTaa                                                                         >  2:191457/1‑239 (MQ=255)
                                                                                                                                                               tCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCACGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTGGTTACACGGCGGGGGTGGCGTAAAGGAACGCCAGGGTTGTACCGATTaa                                                                         >  2:920219/1‑239 (MQ=255)
                                                                                                                                                                     attcgcgGGGCCTGCACAAAAATGATATGCGGGCGGCCGGCGATGCCCAGCGTTACTTCGTTACCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACaaaa                                                                    <  1:211235/235‑1 (MQ=255)
                                                                                                                                                                     atccggtGGCCCGACACAAAAGGCATATGCGCGCGCCTGTCGATGACCAGCGTCACTGCGTTACCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACaaaa                                                                    <  1:954066/232‑1 (MQ=255)
                                                                                                                                                                              gCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAAAACGGTTaa                                                           >  1:805975/1‑238 (MQ=255)
                                                                                                                                                                                 ttCACAAAAAGGATATGCGGGCCCCCGTCGATGACCAGCGTCACTTCTTTACCGGAAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAg                                                       <  1:404168/238‑1 (MQ=255)
                                                                                                                                                                                 tccaaaaaaaGGATTTGCGTGCCTCCGTCGTTCACCCGCCCTCCGTTGTTCTCGATAAACCCTGCCCCCTCCCCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAGCAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAg                                                       <  2:12528/232‑1 (MQ=255)
                                                                                                                                                                                  caacaGAAGGGATTTGCGAGCGCCCGTCAGTCCCCAGCGTCACTTCGTTTCGGGTAAACCTTTCCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGc                                                      <  1:903190/237‑1 (MQ=255)
                                                                                                                                                                                               atGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATAAGCGAGTTGAGGATc                                          >  1:823411/1‑238 (MQ=255)
                                                                                                                                                                                                   gAGCGCCCGTGGAGGCCCGGCTTCATTTCGTTAGCGGAAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGcc                                      <  1:199076/238‑1 (MQ=255)
                                                                                                                                                                                                   gAGCGCCCGGCGGAGCCCAGCTTCACTTGGTTACCGGCAAACCTTTGCGCCGCCTCCAGGCTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGcc                                      <  1:751876/238‑1 (MQ=255)
                                                                                                                                                                                                      cgcCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTACTGGATAGCCAGGTTGTCATCGATTAACAAAATACTGTTAATAAGCGAGTTGAGGATATTCCCCGc                                  >  1:810765/1‑239 (MQ=255)
                                                                                                                                                                                                      cgcCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCggc                                                                                           >  1:764337/1‑181 (MQ=255)
                                                                                                                                                                                                      cgcCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGc                                  >  1:695440/1‑239 (MQ=255)
                                                                                                                                                                                                                   ccAGCGTCACTTCTTTATCGGTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGc                                                      <  1:731773/206‑1 (MQ=255)
                                                                                                                                                                                                                   ccAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGc                                                      >  2:731773/1‑206 (MQ=255)
                                                                                                                                                                                                                   ccAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATAAGCGAGTTGAGGATTTGCCCAGCATCGGGCTGCGt                      >  1:944907/1‑238 (MQ=255)
                                                                                                                                                                                                                      gCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGGTTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCACCATCGGGCTGCGTg                     >  2:205061/1‑236 (MQ=255)
                                                                                                                                                                                                                       cGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAATTACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGGGccgg                 >  1:856397/1‑237 (MQ=255)
                                                                                                                                                                                                                       cGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCGGCATCGGGCTggtgg                     >  1:910416/1‑231 (MQ=255)
                                                                                                                                                                                                                       cGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTg                     >  1:888148/1‑235 (MQ=255)
                                                                                                                                                                                                                        tcctcTTCGTTTCCGATAAACCCTGGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGt                <  1:513088/235‑1 (MQ=255)
                                                                                                                                                                                                                            cTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAgg                                                                                       >  1:979483/1‑164 (MQ=255)
                                                                                                                                                                                                                               cGTTAGCGGTAACACCGTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATa         <  1:878997/239‑1 (MQ=255)
                                                                                                                                                                                                                                    tgttaaaacCCTTCCCCCGCCTCCAATCTTCTTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGGTGCGGTAACGGTGTACCAAACTATTTGCGGGATCTTTGGGCGAGCAGGTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAATCa    <  2:72864/234‑1 (MQ=255)
                                                                                                                                                                                                                                    tCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGGTCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCa    >  1:605851/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCTTAAAGCAg   >  1:129075/1‑239 (MQ=255)
                                                                                                                                                                                                                                      ggTAAACCCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGt  <  1:407989/239‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
CTGCGCCGCGCCACGTAAACCGCCAAGCGGATTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTACCAAACAATTTGCGGGAGCTTTGGGCGAGCAGTTGTTGCGCGGCAGGGTTGGCGTAATGGATCGCCAGGTTGTCATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGT  >  NC_000913/4055881‑4056347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: