Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,242,022 C→A 12.2% C78F (TGC→TTC)  xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,242,0220CA12.2% 214.7 / 2.0 81C78F (TGC→TTC) xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base C (44/24);  new base A (2/10);  total (46/35)
Fisher's exact test for biased strand distribution p-value = 3.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCA  >  NC_000913/4241794‑4242253
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        
aGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCTGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGCCGACCGAAGCGGTTACTGCAATAaccacc                                                                                                                                                                                                                                >  1:132819/1‑239 (MQ=255)
               aTTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTCCACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGAAAAAACCACCGAGGGCACCCCCcat                                                                                                                                                                                                                >  2:235021/1‑239 (MQ=255)
                 tGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                                                                                                                              >  1:129737/1‑239 (MQ=255)
                 tGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCGCCgttga                                                                                                                                                                                                              >  1:240523/1‑239 (MQ=255)
                  ggCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACCCCAGAAATAAAAAACAGGACAGCAGAAATCTTAAGTGAATACCAACGACCGAAACGGTACATGCAATAACCACCAAGGGACCCGCCgaa                                                                                                                                                                                                                >  2:638587/1‑235 (MQ=255)
                    cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGc                                                                                                                                                                                                           >  1:285941/1‑239 (MQ=255)
                      agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACGACGACCGAAACGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGCa                                                                                                                                                                                                          >  1:612601/1‑238 (MQ=255)
                        agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGGTGATGCAAcc                                                                                                                                                                                                       >  1:735725/1‑239 (MQ=255)
                        agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGAACCGCCGATGATGCAAcc                                                                                                                                                                                                       >  1:927085/1‑239 (MQ=255)
                                     aaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCaacacgctg                                                                                                                                                                                          >  1:651575/1‑231 (MQ=255)
                                           cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCa                                                                                                                                                                                                  >  1:830215/1‑225 (MQ=255)
                                              aaaccccGCAAATATTGGGAAAAATAAAAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGCCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                                                 <  1:430856/234‑1 (MQ=255)
                                               aaTACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTAACTGCACTAACCCCCAAGGGCACCCCCAAGGATGCAACCAACAAAAGCCCGGGCCCCCCaaa                                                                                                                                                                                >  2:372551/1‑239 (MQ=255)
                                                 tACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaaaa                                                                                                                                                                               >  1:249921/1‑238 (MQ=255)
                                                 tACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAACAGGTTACCGCAATAACCACCGAGGGCACCGCCGAGAGGGCAACCAATCAGAACGCTGGCCACCAAAAAc                                                                                                                                                                              >  2:704127/1‑239 (MQ=255)
                                                      ccAATAATGCGATAAATAACAAATTCCGGGACATAAACTGCAAGATAAACAGGCACAGTGTTGTCCGGGTTTATCGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACCACAGAAATAAAAAAAAGGACAGCAGCAATCTTAAGGGAATCACGACGACGGAAGCGGGTCCTGAAATAACCACCGCGGGCAACGCCGTTGATGACAGAAATCAAAGCGCTGACACCGAAAAAcccacc                                                                                                                                                                         >  2:75505/1‑236 (MQ=255)
                                                         ataagGCGTAAAAAAACAAATTCCGGCACAAACCCTGCCAGACAACCAGGAAAAGGTTGGCCCGGTTTAATAGAGTTAAAACCAGGTCTTGCCCAGCCAAAACCTCCCCAAGAAAAAAAAAACAGCACAGCAGCAATCTAAAGTGAATCACGCCGCCCGAAGCTTTTCATTCAATACCCCCCGAGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAg                                                                                                                                                                      <  2:699577/239‑1 (MQ=255)
                                                                 ataaataaCAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTg                                                                                                                                                               >  1:32388/1‑238 (MQ=255)
                                                                 ataaataaCAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTg                                                                                                                                                               >  1:550736/1‑238 (MQ=255)
                                                                                   gacaacaCCTGCCAGAAAAACAGGCACAGTGTTGTCCGGGTTTAAAGAGGAAAACCCAAGTCCGGGCCAGGCAAAACCTACACCAAAAAAAAAAAACAGGACAGCAGCAATCTAAAGTGAATCACGACGACCGAAGGGGTTCCGGCAATACCCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAg                                                                                                                                             <  2:927085/232‑1 (MQ=255)
                                                                                       tAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTtg                                                                                                                                        >  1:18389/1‑239 (MQ=255)
                                                                                                      ccAGGCAAGTTTTTTCCGGGTTTAAAGGAGATAAAACCAAGTTCGTGACAGGAAAAACCAACCCCAAAAAAAAAAGCAGGCCAGCAGAAACTTTAAGTGAACCCCGACGACCGAAGGGGTGCCGGAAATAACCCCCGAGGCAACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa                                                                                                                          <  2:460809/236‑1 (MQ=255)
                                                                                                           ccaaGTTTTGTCCGGGTTAAAAGAGGTAAAACCAAGTTCTGGCAAGGCAGACCCTACACAAAAAAAAAAAAACAGGCCATCAGAAACCTTAAGTGAATCACGACGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACgg                                                                                                                       <  1:129187/233‑1 (MQ=255)
                                                                                                             caGTGTTGTCCGGGATTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCAAACAGGGAGTTGGCAAGGGATTCACTAAAGATTTGTGGACAAACAAAAACggaaatg                                                                                                                  >  2:129187/1‑234 (MQ=255)
                                                                                                              aGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                                                                                                                     >  1:642864/1‑124 (MQ=255)
                                                                                                              aGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                                                                                                                     <  2:642864/124‑1 (MQ=255)
                                                                                                               tgtttgcCCGGGTTTAAAGGGGTAAACCCAAGTTCGGGCAAGGAAGACCCTACCCCAAAAAAAAAAAACAGGAAAGCAGCAATCTAAAGTGAATCCCGACGACCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAg                                                                                                                <  1:372551/236‑1 (MQ=255)
                                                                                                                tgttgtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGGATTGAGt                                                                                                               >  1:147258/1‑239 (MQ=255)
                                                                                                                tggtccccGTGTTTAAAAAGTAAAAACCAAGTTTGGAGCAGGACAAACCACCCCCAAAAAAAAAAAACAGGAAACCGCCAATCTTAGGGAAACCACGCCACCCGAAGGGGTTACGGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt                                                                                                               <  2:150525/233‑1 (MQ=255)
                                                                                                                 ggtgtCGGGGTTTAGAGAGGAAAACCCAATTTCGGGCCAGGCAAAACCTACACCAAAAAAAAAAAACAGGACAACAGCAATCTTAAGTGAATCCCGGCGACCGAAGCGGTTACTGCAAAACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGt                                                                                                               <  2:147258/236‑1 (MQ=255)
                                                                                                                    gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGAATTGGGTGAc                                                                                                            >  1:409822/1‑238 (MQ=255)
                                                                                                                    gtCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAAAAAAGACGGTATTGAGTGAc                                                                                                            >  1:779453/1‑238 (MQ=255)
                                                                                                                     tCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTATGGAGCAACAAAGACGGTATTGAGTGACTc                                                                                                          >  1:650230/1‑239 (MQ=255)
                                                                                                                     tCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGGGCAACAAAGACGGTATTGAg                                                                                                                >  1:460809/1‑232 (MQ=255)
                                                                                                                       cGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGGGGGACTCa                                                                                                         >  1:575709/1‑238 (MQ=255)
                                                                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTCACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGTGCAACAAAGACGGTATTGAGTGACTCACCAg                                                                                                     >  1:303998/1‑239 (MQ=255)
                                                                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGAGGATTCACTTACGTTTTGTGGAGCAACCAAGACGGTATTGAGTGACTCAACa                                                                                                      >  1:846930/1‑238 (MQ=255)
                                                                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCCAACAGGGAGTTGGCAGCGGAATCACTTAAGTTTTGTGGGGCAACAAAAAAGGTATTGAAGGACCCAAAAg                                                                                                     >  2:442090/1‑239 (MQ=255)
                                                                                                                          gTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATAACGACGACCGAAGCGGGTACTGCAATAACCACCGAGGGCACCGCCGATGATGAAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGCGTTGGAAGCGGAATACCTTCAGATTGATGGAGCAAAAAAAACGGAATTATGTAACTCAAAAg                                                                                                     >  2:773683/1‑239 (MQ=255)
                                                                                                                                      gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCCCGCAAAACCCTAACAGGGGGTTGGCAGCGGATTCCCTTAAGATTTGTGGGGCAACAAAAACGGGATTGATGGACCAAACAGAACCGGaaaa                                                                                           >  2:695606/1‑238 (MQ=255)
                                                                                                                                      gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGATTTGTGGAGCAACAAAAACGGAATTGAGGGACTACAAAAAACCGGaaaa                                                                                           >  2:361946/1‑238 (MQ=255)
                                                                                                                                      gTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACCTAAGCTTTGTGGCGAAACAAAGACGGTCTTGAGGGACTCAAAAGAACCGGaaaa                                                                                           >  2:313176/1‑238 (MQ=255)
                                                                                                                                           aCCAAGTTCAGGCCGGCCAAACCCTACACAGAAAAAAAAAAACAGGCAAGCAACAATCGAAAGTGAATCAGGACGACCGAAGCGTTTCCTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAAcgg                                                                                      <  1:704127/239‑1 (MQ=255)
                                                                                                                                                gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCACTaa                                                                                                                                                                                                                                     >  2:461008/1‑92 (MQ=255)
                                                                                                                                                gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                                                                                                                                                                                                                                     <  1:461008/92‑1 (MQ=255)
                                                                                                                                                gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGCGGGCACCGCCGATGATGCAACCAATCAGAGCGTTGGCCCCGCAAACCCCTAAAAGGGAATTGGCAGCGGCTTCCCTTAAGTTTTGTGGAGCAACAAAACCGGTATTGAGAGACCCAAACGAACCGGaaaaaaaaggggag                                                                                 >  2:167046/1‑231 (MQ=255)
                                                                                                                                                gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAAcggcgggg                                                                                 >  1:679956/1‑237 (MQ=255)
                                                                                                                                                gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAAGGATTCAACAGTACCGGAAATAACGGCGgtg                                                                                 >  1:609679/1‑239 (MQ=255)
                                                                                                                                                        acgcaaGACCCTACACAAAAAAAAAAAAACAGTACAGACCCACTCTAAGGCGAACCCCGGCCACCAAAGCGGTTACAGCAATACCCCCCGGGGTACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGcc                                                                         <  2:249921/234‑1 (MQ=255)
                                                                                                                                                         gggCAGACCCTACAACAGAAAAAAAAAACAGAAAAGCAACAATCTAAGGGAAACCACGGAGACCGAGCGGTTTACGAAAATACCCACAGAGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCa                                                                        <  2:32388/238‑1 (MQ=255)
                                                                                                                                                                 ccgccacCAAAAAAAAAAAACCGGGAAGCAGCAATCTAAAGGAAATCACCACGACCGAGGCGGTTACGGAAAAACCCCCGGGGGCACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAAtaataa                                                                <  2:132819/235‑1 (MQ=255)
                                                                                                                                                                  ctcccccAGAAAAAAAAAACAGGCCACCGGCAATCTAAAGGAAAGCCGGCGCACCGAGGCGTTTACGGCAAACGCCACCGGGGGCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGGCGTAGCCAAATAATaaa                                                               <  2:438750/234‑1 (MQ=255)
                                                                                                                                                                  ctccccaaaaaaaaaaaCACAGGAACGCAGGAATCTTAAGGAACTCCGCAAGACCGACGCGGTTCATGAATTACCCCCGGAGGGACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                               <  2:575709/230‑1 (MQ=255)
                                                                                                                                                                  cTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTAAACCACGACGACGGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                               <  1:695606/239‑1 (MQ=255)
                                                                                                                                                                  cTACACCAAAAAAAAAAAACAGGACAGCAGCAAGATTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                               <  1:638587/239‑1 (MQ=255)
                                                                                                                                                                  cTACACAAGAAAAAAAAAACGGGCAAGCAGCAATCTAAAGTGAATCACGCCGACAGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                               <  1:634127/239‑1 (MQ=255)
                                                                                                                                                                    acacCAGAAATAAAAAACAGGACAGCAGCAATTTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAacc                                                             <  1:361946/239‑1 (MQ=255)
                                                                                                                                                                     cacCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGGTTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGCAATAACGGCGGTGTTGGAGCCAAATAATaaaaa                                                             >  1:974572/1‑236 (MQ=255)
                                                                                                                                                                     cacCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGGGTCGGGGCCAAACAATaaaac                                                             >  1:903035/1‑236 (MQ=255)
                                                                                                                                                                         aGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTgg                                                                                                                                                                                         >  1:1010335/1‑110 (MQ=255)
                                                                                                                                                                             aaaaaaaaCCGGACAGAAGAACTTTAAAGTGACGCCCGGCGACCGAAGCGGTTACTGCAAGACCCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGt                                                    <  2:18389/237‑1 (MQ=255)
                                                                                                                                                                              aaaaaaaCAGGACAGCGAGAGACTAAAGGGAAACACGACCACGGAAGGGTTTATGGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTa                                                   <  1:343208/238‑1 (MQ=255)
                                                                                                                                                                                        gACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGGTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGGCTCGTAGCCCAAAAATTAAACACCTAATTTatgaacacaag                                         >  1:68027/1‑229 (MQ=255)
                                                                                                                                                                                                       aaGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAAAAATAAACCCCCCAATGTAGCGACGAAGGGAATCGGAAatatat                          >  1:766004/1‑239 (MQ=255)
                                                                                                                                                                                                       aaGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGGCGGAGCCCAATAACAAAACACCTAATGAAAAAACTAACGTAATCCAAAatatat                          >  1:386091/1‑239 (MQ=255)
                                                                                                                                                                                                       aaGTAAATCCCGACGACCGAAGGGGTTACTGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGa                                  <  1:819753/231‑1 (MQ=255)
                                                                                                                                                                                                           gaaccccgacgaCCGAAGCGGTTACCGCAAAACCCACCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                       <  1:715431/232‑1 (MQ=255)
                                                                                                                                                                                                           gAACCACGACGACCGAAGCGGTTACTGCAATAACCCCGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                       <  1:334650/238‑1 (MQ=255)
                                                                                                                                                                                                           aaacccggacgccGGACGCGGTTCCTGAAAACCCCCCCGGGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                       <  1:432048/231‑1 (MQ=255)
                                                                                                                                                                                                           aaaTCACGACGACCAAAGGGTTTACTGCAAAACCCCCCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACt                      <  1:442090/238‑1 (MQ=255)
                                                                                                                                                                                                            aaTCCCGAGCACCGAAGCGGTTACGGAAATAACCCCCGGGGACACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                       <  1:961998/237‑1 (MQ=255)
                                                                                                                                                                                                            aaTCCCGAAGACCGAAGCGGTTTCCACAAAACCCCCCGAGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                       <  1:773683/237‑1 (MQ=255)
                                                                                                                                                                                                             aTCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAAAAGTACCGGAAATAACGGCGGTGTCGTAACCCCATAATTAAACAACCCATTAAACGACTAAAGTAAACGGAAATATATAAATgg                    >  1:745685/1‑239 (MQ=255)
                                                                                                                                                                                                               cACGCCGCCCGAAGCGTTTCCTGAAATAACCCCCGAGGCCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGAAAACCCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAACCCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGaa                  <  1:551376/239‑1 (MQ=255)
                                                                                                                                                                                                                       ccacgaggggtttACTGAAAAACCCCCCGGGGCCCCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATAc            <  2:779453/228‑1 (MQ=255)
                                                                                                                                                                                                                        ccGAAGAGTTTAATGAAATACCCCCCAGGGGCACCGCCGAGGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCGAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTg          <  1:813521/239‑1 (MQ=255)
                                                                                                                                                                                                                        cacatgCGGTTACTCAAATAACCCCGGGGGGACCCGACGATGAGGAAACCAATAAGGGCGCTGGCCCAGCAAAACCCTAACAGGGAGGTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTg          <  2:303998/234‑1 (MQ=255)
                                                                                                                                                                                                                                 gctACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGGCGTAGCCAAAAAAAAAACCACCCAATGTACGAACTAAGGGAATACAAAAAATATAAAAGGAATTTAACTGGGGattta  >  1:197279/3‑237 (MQ=255)
                                                                                                                                                                                                                                      ggCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGaa                  <  1:34728/217‑1 (MQ=255)
                                                                                                                                                                                                                                       gCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGa                                                                                                                                    <  2:140189/103‑1 (MQ=255)
                                                                                                                                                                                                                                       gCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGa                                                                                                                                    >  1:140189/1‑103 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        
AGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCA  >  NC_000913/4241794‑4242253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: