Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,530,174 | T→A | 12.0% | I24F (ATC→TTC) | sgcC ← | putative PTS system EIIC permease component |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,530,174 | 0 | T | A | 12.0% | 236.2 / 2.7 | 84 | I24F (ATC→TTC) | sgcC | putative PTS system EIIC permease component |
Reads supporting (aligned to +/- strand): ref base T (49/23); new base A (2/9); total (51/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.43e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGAGGGAT > NC_000913/4529936‑4530407 | ttGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGCCAAAACCAATCCCAACCGTTACCCCTGCCTTAATAGCCTGCAGCCAGGGGAT‑GCGG‑AAGAt > 1:812410/1‑239 (MQ=255) tGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGTTa > 1:240757/1‑239 (MQ=255) aaaaaTAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCATAGACATGCAGGTCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGCTCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑ACGG‑AAGCTAAGGCCa > 1:774049/1‑238 (MQ=255) aaaaaTAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATAACGATCACCAGTCCCCAACCGCACAAACCAATCCCAACCGTTCACCCAGCCTTAATCCGCCGAACCCAGGGGATGGCGA‑AAGAAAAgcaa > 2:81315/1‑235 (MQ=255) aagatcTGGGATAATGTTGGCGCCAAGGGGTGGCGAATAACCAACGCCGGAGGCAGGGCCCGCACCAACGGCAAAGACATGCAGGGTAAGGCCGAAACGCTCAATCAACACTTTGATAGGGGGACTGAGGCTTTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAAGCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCa < 2:812556/234‑1 (MQ=255) aaTGACTGGGATAATCATGGCGCCAATGGCTTTCGCATAACCAAGGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCCCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCa < 1:18000/239‑1 (MQ=255) accggggATAATCATGGGCCCAATGGCTGACCCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGGCAAAGGCATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACg < 2:420537/234‑1 (MQ=255) aCTGGGATAATCAGGGCACCAATGGCTGGCGCAGAACCAACGCCGGAGGCAGGGCCTGCGCCAGCGTCAAAGACAGGCAGGGCTAGGCCGAAACGCTCAATCATCGCTGTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCTCCAGTCCCATACCGACATATCCAATCCCAACCGTTTCCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGAt < 1:300557/239‑1 (MQ=255) gATAACCATGGCCCCAATGCGTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGGCAAAGACATGCAGGTGTAGGCCGAAACGCTCAAGCAGCCCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCatg < 2:293301/238‑1 (MQ=255) aaTCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCACTCACGATCCtg‑atga > 1:420537/1‑239 (MQ=255) tCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATa < 1:72296/239‑1 (MQ=255) ggCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCCTGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCCCGATCATG‑TTGATAggg > 1:783113/1‑238 (MQ=255) ggCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCCCGATCATG‑CTGATAggg > 1:345936/1‑238 (MQ=255) ggCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCCTG‑ATGATAggg > 1:1011026/1‑238 (MQ=255) gcgcCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAgg > 1:789968/1‑213 (MQ=255) aagggCTGGCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTAAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGaaaa < 1:183175/236‑1 (MQ=255) aaTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCCCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCCATCCCATACCGCCAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGTTAAGGCCAATCACGATCAtg‑atga > 1:669267/1‑227 (MQ=255) cgtccatcaaCCCACCCGGGGGGCGGGGCCTGCCCCAAGGCAAAAGGCATGGAGGGTTGGGCCAAACCCCCCAATCACCCTTTTGATAGGGGGACTGGGGTTGCCAACCGCCATGCCGATCCCCAGCCCAATCCCGAAAAAACCAATCCAAACCTTTCCCCCTGCCTTAACCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑c < 2:977775/231‑1 (MQ=255) aaCCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCcaga > 1:769775/1‑239 (MQ=255) cGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTTCCGCCAGGGGAT‑GCGG‑AAGATAAGGCCAACCACGATCATA‑ATGATAGGGCCGAAACCGGTA‑CCCCCGGAAGACaag > 2:426738/1‑237 (MQ=255) aGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCTATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCACCCAGGGGAG‑ACGG‑CAGTAAAGACCAAACACGATCATG‑GTTATAGGGCCGAAACCAGTA‑CCGCCAGAAGACAgaggataa > 2:877247/1‑232 (MQ=255) tGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCCCGATCATG‑ATGATAGGGACGAAAACGGGA‑CCGCCGAGAGACAGGATGTAATCAAACATaa > 1:886580/1‑239 (MQ=255) cccAACGTAAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCAATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGc < 1:532611/238‑1 (MQ=255) aaCGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCCCGATCATG‑TTGCTAGGGACGAAAACGGAA‑CCGCCGGGAGACAGGAGGTAAACAAAAAAAAAGccc > 2:778162/1‑237 (MQ=255) aaCGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATAACGATCATG‑ATGTTAGGGACGAAAACGGTA‑CCGCCGGGTGACAGGATGTAATAAAACATAATGcct > 1:890005/1‑238 (MQ=255) aaGACATGAGGGGCTAGGCCGAAAGGCTCAATCATCACTTTGATAGGGGGACGGAGGCTGTCGATCGCAATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTAt < 1:767379/239‑1 (MQ=255) aaGACAGGCAGGGCTAGGCCGAACCGCTAAACCATCCCTGTGATAGGGGGACTGAGGCTGCCGATCGCCATGACGATCACCAGTCCCATACCGACAAACCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTAt < 1:426738/239‑1 (MQ=255) gCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCAc < 2:122424/160‑1 (MQ=255) gCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCAc > 1:122424/1‑160 (MQ=255) cTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATCAGGCCAATCCCGATCATG‑TGGATAGGGCCGAAAACGGTA‑CCCCCGCGAGACAGGAGGTAATCAAACATAATGCCACCTTTTATTGTGTTaaacag > 2:198043/1‑234 (MQ=255) ggCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGAAATCAAAAATAAAGCCACCCTTTATTGAGTTaaaaaag > 2:471078/1‑231 (MQ=255) ggCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATC‑ATGATAGGGACGAAAACGGAA‑CCGCCGAGAGCAAGGATGTAATCAAACATAATACCTCCTTTTATTCAGTTAACAAAGCCt > 1:345946/1‑239 (MQ=255) aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ACGATAGGGACGAAAACGGTA‑CCGCCGAGCGACAGGATGAAATCAAACATAAAGCCTCCCTTTATTGAGTTAACAGAGGCttagtt > 2:716353/1‑235 (MQ=255) aaaCGCTCAATCATCACTTTGATAGCGGGACTGCGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGAAGCCAGGGCAT‑GCGG‑AAGATAAGGCCAATACTGATCATG‑CTGCTAGGGCAGAAAACGGTT‑ACGCCGAGGGACAGGATGTAATCAACACTAATGCCTCCTTTTATTGAGTGAACAGACACTTAAtt > 2:237467/1‑239 (MQ=255) ccGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGACCCCCAGTCCCATACCGAAAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ACGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttg < 1:716353/238‑1 (MQ=255) caCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAAcgc > 1:281521/1‑239 (MQ=255) gATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAAt < 1:760311/123‑1 (MQ=255) gATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAAt > 2:760311/1‑123 (MQ=255) ggggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAAAGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTg > 1:97156/1‑238 (MQ=255) ggaaCTGAGGCTTTCGATCGCAAGAACGATCCCCAGTCCCATACCGACAAACCCAATCCCAACCGTTCCCCTTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa < 1:377599/235‑1 (MQ=255) ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACa > 1:57142/1‑201 (MQ=255) ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGAt > 1:861258/1‑238 (MQ=255) ggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGCAATCAAACATAATGACTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAATGCGTCGTCGTTGAt > 1:702973/1‑238 (MQ=255) aCTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGAA‑CCGCCGAGAGACAGGATGTAATCAACCATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGCTTTGTTGTTTTAACGGCTCGCCGTTGATc > 1:485688/1‑237 (MQ=255) tGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCCTTTATTGAGTTAAAAGAGCCTTGATTTTTTGTTTTAACGCGGCGTCGTTGATACCtt > 2:377599/1‑238 (MQ=255) aGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGAAT‑GCGG‑AAAATAAGGCCAATCACGATCAGG‑TGGAAAGGGACGAAAAGGGTA‑CCGCGGCGACACAGACTGTAATCAAACAAAATGCCCCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTTTTTTAACGCGTCGCCGTTAATCCCtgtg > 2:269325/1‑239 (MQ=255) gCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGAt > 1:262637/1‑114 (MQ=255) gCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGAt < 2:262637/114‑1 (MQ=255) gCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAAAAGAGCCTTGATTTGTTGTTTTAAAGCCTCTTCGTTTATCCCtgtggc > 2:640630/1‑237 (MQ=255) gTCGATCGCCATGACGATCACCAGTCCCATACCGGCAAAACCAATCCCAACCTTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATAATG‑ATGATAGGGACGAAAACGGTA‑CCGCCgagaga < 1:738330/147‑1 (MQ=255) gTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCgagaga > 2:738330/1‑147 (MQ=255) tGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACAATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGGGACCGGACGTAAAAAAACATAATGCATCCTTTTATTGAGTTAAAAGAGCCTTGATTTGGTGTTTTAACGCGTAGTGGTTGATCCCTGTGAGCAGGGGGGGGCCAt > 2:451233/1‑239 (MQ=255) gccgccccccACTCCCAGACCGACAAAACCCAGCCCACACGTCCCCCCTGCCTTGCTCGCCGGCAGCCGGGGGAT‑GGGG‑AAGAAAAGGCTAATCACGTGCATG‑AGTCTAGTAACGAAACCGGGA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt < 2:1001681/231‑1 (MQ=255) aaaagggcgttgTCCCATGCCGAAAAACACATGCCCCAGCGTCGCCCACGCCATAATCCTCTGAAACAAGGGGGA‑GCTG‑ACGACACGGCCAACCACGAGCATG‑ATGGTAGGGACGAAGAGGGGA‑CCGGAGAGAGACAGGATGTAATCAAACATAATGCCGCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCAtt < 2:201770/228‑1 (MQ=255) ccccAGTCCCATCCCGAAAAACCCAACCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAggg < 1:979805/221‑1 (MQ=255) gTCCCATCCCGAAAAAACCAATCCCAACCGTTACCCCTCTCTTAATCGCCTGCGGCCCGGGGAT‑GGGG‑AAGATAAGGCCAATCCCGATAATG‑ATGGTAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGa < 1:269325/239‑1 (MQ=255) gTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACTCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGa > 1:1017982/1‑239 (MQ=255) gTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAGGCGTAGGGa > 1:672221/1‑239 (MQ=255) cgtctccaaaaaagCCGATCCCAACCTGTACCCGTGCTAGAAGCGCCTAGGGTCAGGGTAT‑GCGG‑AAGAAAAGGCAAATCACGTTCATG‑ATTATAGGGAGAAAAACGGTC‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGAGTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGACGc < 2:801701/229‑1 (MQ=255) cATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGcc > 1:687319/1‑239 (MQ=255) caaaaaaaCCAATCCAAACCGTTCCCCCTGCCTTAACCCCCTGCAGCCGGGGAAT‑GGGA‑AATATAAGGCAAATCAAGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAAt < 1:876335/235‑1 (MQ=255) aCAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTAtt > 1:958426/1‑147 (MQ=255) aCAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTTTTTTTTTAACGCGTCGTGGTTTTTCCCTGTGAGCAGGGCGGGGCCATTAAGCGTAGGGTGGCCGAAAACa > 2:971229/1‑239 (MQ=255) aCAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATc > 1:369621/1‑238 (MQ=255) aCAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATc > 1:207851/1‑238 (MQ=255) aCAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGCCGGCGCCATTAAGCGTAggg > 1:201770/1‑227 (MQ=255) aaTCCCAACCGTTACCCCGGCCTAAAGCCCCGGCAGCCAGGGGAT‑GCGA‑AAGAAAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGt < 1:442391/239‑1 (MQ=255) ccctttcccccTGTCTTGACCGTCGGAAGCCAGGGAAG‑GCGG‑AAAGAAAGGACAACCGCGATCAAG‑ATAAAAGGAACGAAACGGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATg < 2:281521/232‑1 (MQ=255) aCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGCTTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGGAGGGATGCCGTAATAACTGTTGGGTCGCATg > 2:24873/1‑239 (MQ=255) ctttcccctTGCGTTATTCGCGTG‑AGCCCGGGGAT‑GGGGAAAGCAAAGGCCAATCAGGATCATG‑ATTAGGGGGACGAAACCGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGa < 2:369621/234‑1 (MQ=255) tACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTGGCGTGGAGGt > 1:267487/1‑239 (MQ=255) aCCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGtt > 1:912644/1‑239 (MQ=255) tGCCTAAACCGCCTGCAGCCGGGGGAG‑GGGG‑AAGAAAAGGCAAATCACGATCAGG‑ATGATAGGGACGAAACCGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAAt < 1:198043/239‑1 (MQ=255) ctccTTAAGCGCTGGGAGCGGGGGGAT‑GCGG‑AAAAAAAGGCAAACCAGGAT‑ATGTATGAAAGGGACAAAAAGGGTA‑CCGACGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAAt < 2:861258/237‑1 (MQ=255) gCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGTCGACGCCATTAAGCGTAGGGATGCCGCAATCCCTGTTGGTTCGCGTGGGGGTTACaaaa > 2:675648/1‑237 (MQ=255) gCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGGGGTTACAATa > 1:751203/1‑239 (MQ=255) gCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTAATCCCTGTAAGCAGGGCGGCGCCATTAAGCGTAGGGATGCGGAAATCCCTGTTGGTTCCCATGGGGGtaaaaaaa > 2:827538/1‑232 (MQ=255) gCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAACCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATa > 1:47482/1‑239 (MQ=255) cctccggCCTGGGGAT‑GGGG‑AAAAAAAGGCCAAACACGAACATT‑ATGGTAGGGACGAAAACGCTA‑CCGCCGAGAGACAGGATGTAAAAAAACATAATGCCTCCTTTTATTGAGTCAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATAc < 2:1017982/233‑1 (MQ=255) ctgaagcagggggAG‑GCGG‑AAGATAAGGCCAATCGCGATAATG‑TTGATAGGGACAAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATAc < 1:192511/229‑1 (MQ=255) cTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAGCAGAGCCTTGATTTGGTGTTTTAGCGCGTCGTCGTTGATCCCTGTGAGCAGGGGGGCGCCATTAAGCGTAGGGATGCCGTAATCCCTTTTGGTTCGCATGGGGGTTAAAAAAAAGACCATAcc > 2:270376/1‑239 (MQ=255) aataTAAGGACAATCCCGATCATG‑ATGAGAGGGAC‑AAAACGGGACCCGCCTGGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGATTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGAGGGAt < 1:451233/235‑1 (MQ=255) aaGAAAAGGCCAATCCCGATAAGG‑ATAAAGGGGAAGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGAGGGAt < 1:471078/238‑1 (MQ=255) gATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa < 1:336047/133‑1 (MQ=255) gATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa > 2:336047/1‑133 (MQ=255) | TTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGAT‑GCGG‑AAGATAAGGCCAATCACGATCATG‑ATGATAGGGACGAAAACGGTA‑CCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAGGGCGGCGCCATTAAGCGTAGGGATGCCGTAATCACTGTTGGTTCGCATGGAGGTTACAATAAGGTCCATACCGTTACAGTTAAGAGGGAT > NC_000913/4529936‑4530407 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |