Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,588,079 A→C 20.1% W263G (TGG→GGG)  yjiA ← metal‑binding GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,588,0790AC20.1% 175.6 / 4.6 79W263G (TGG→GGG) yjiAmetal‑binding GTPase
Reads supporting (aligned to +/- strand):  ref base A (21/39);  new base C (12/7);  total (33/46)
Fisher's exact test for biased strand distribution p-value = 3.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGGACTATTTTGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGA  >  NC_000913/4587845‑4588312
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            
tagtgACTATTTTGAGTTAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAg                                                                                                                                                                                                                                           <  1:255214/234‑1 (MQ=255)
 ttGGCCTTTTTTGGATGAAATATCAGGCCGGGTGCGGCTGCGCCTTGTCCGGCCCATAACCCCTTACTTCCTCAACCCCGGAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCa                                                                                                                                                                                                                                         <  2:32900/238‑1 (MQ=255)
 atGGTCTATTTTGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCa                                                                                                                                                                                                                                         <  1:246588/239‑1 (MQ=255)
     tcttttttGTGTGAAATCCTGGGCCGTGGGCGGCAGCGCCTTAGCCGGCCCATAACCCCGTACTTGCTCAACCCCGCAAACACGACCCGAAGTCCTTCCTCCGGCAGCTGGATCCCGATAATCACCATCGGGCTATGGGGTGTTTCCTCGGCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCATGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCAtac                                                                                                                                                                                                                                      <  2:534331/234‑3 (MQ=255)
              aGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTTTCCCAGTCGGCGCTGTAGAGGGCCTGGACGCCCTGGAACAGCAGGCGGTTAGGGTTGCCGTCCAACCCCAAGAACCCTTTGTaaaa                                                                                                                                                                                                                           >  2:761337/1‑237 (MQ=255)
                   aaTATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTTGCCGGCAATCCCCACCATCCCTTTGTAACggtaca                                                                                                                                                                                                                      >  1:634313/1‑234 (MQ=255)
                          ggCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTTGCCGGCAATCCCCAGCCTCCCTTTGTAACGTaaccggtttt                                                                                                                                                                                                                >  1:770866/1‑229 (MQ=255)
                          ggCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAAGTTTGCCGTCAATCCCCAGCCTCCCTTTTTAACGTAGCAGtttta                                                                                                                                                                                                                >  1:20244/1‑236 (MQ=255)
                                     gCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGGGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTACGTTGGCCGGCAATCCCCCCCAACCCATTGTAACGGAAGAGGTTATCCGCCGAccccc                                                                                                                                                                                                    >  2:915820/1‑238 (MQ=255)
                                         cgcCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGCGGCCCTGGAACAGCAGGCGGTTAGGTTCCCCGTCAATCCCAAGCATCCCCTTGCAAGCACGCAGGTTTTCCCCCGACTcccgac                                                                                                                                                                                                 >  1:533443/1‑234 (MQ=255)
                                         cgcCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCCCCGCATCCCTTTTGAACGTAGCCGGTTATACGCCGCATCcagcc                                                                                                                                                                                                 >  1:167295/1‑237 (MQ=255)
                                          gcCTTACCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCcagcagc                                                                                                                                                                                               <  1:408008/239‑1 (MQ=255)
                                          ccctctcccGCCCCCTAACCCTTTTCTTCCTCACCCCCCCAAACCCAGCCCGAATCCTTTCCTCCGGAAGCTGGATCCCGATAAACCCCCTCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCcagcagc                                                                                                                                                                                               <  2:785828/232‑1 (MQ=255)
                                                      ccATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGGTAGGTTCGCCGGCAATCCCCAGCATCCCCTTGTAACGTAGCAGTTTTTCCGCCGACCCCCGCAGCCGGTTTTccct                                                                                                                                                                                    >  1:463773/1‑236 (MQ=255)
                                                            ccccTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCa                                                                                                                                                                                                                                         <  1:569003/180‑1 (MQ=255)
                                                               cTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCCCAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGGACCCCGCAGCAGGGTTTCCATCAAGCGGGaa                                                                                                                                                                          >  1:527231/1‑239 (MQ=255)
                                                               cTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCCCCCACTCCAGCAGCAGGGTTTCCATCCCCCgggca                                                                                                                                                                          >  1:975300/1‑237 (MQ=255)
                                                                 tacccccccAACCCCGCAACCGCACCCCGAATCCCTGCCTCCGGCAGCTGGAACCCGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAAc                                                                                                                                                                        <  2:634313/231‑1 (MQ=255)
                                                                 tACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGGTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGGTTATCCGCCGACTCCAGCAGCAGGGTTTCCATCACGCgggga                                                                                                                                                                          >  1:26835/1‑235 (MQ=255)
                                                                     tCCTCAACCCCGCAAACGCAGCCCTAATCTCTACCTCCGGCAGCTGGATCCCGATAAACCCCAGCGTTCTATGCGGTTTTTCATCGCCCCACGGCCTTTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCg                                                                                                                                                                    <  1:255871/239‑1 (MQ=255)
                                                                     tCCTAAACCCCGCAAACGCAGCCCGAATCCCTTCCTCCGGCAGCTGCATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCg                                                                                                                                                                    <  1:121974/239‑1 (MQ=255)
                                                                     cccccacccccccAAACCCAGCCCCAATCTCTTCCCCCGGCAGCTGGATCCCGATAAACCCCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCg                                                                                                                                                                    <  2:26835/232‑1 (MQ=255)
                                                                     ccccaccccccccAAACGCCGCCCCAATTCGTTCCTCCGGCCGCTGGATCCCGAAAAACCCCATGGTGCTATGGGGTTTTCCCTCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCg                                                                                                                                                                    <  2:20244/232‑1 (MQ=255)
                                                                      ccTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTa                                                                                                                                                                                                                              <  1:354453/180‑1 (MQ=255)
                                                                         cAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTTTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCCGGGTTTCCATCACGCGGGAAAATTCCCTGa                                                                                                                                                                >  1:875501/1‑239 (MQ=255)
                                                                               cGCAAACCCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTTCTATGCGGTTTTCCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTa                                                                                                                                                         <  1:126928/239‑1 (MQ=255)
                                                                                    aCGCAGCCCGAAGCGCCTCCTCCGGCAGCCGGATCCCGATAAACACCATCGTGCTATGCGGTTTCTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCggg                                                                                                                                                    <  1:341685/239‑1 (MQ=255)
                                                                                                        ccgggCAGCTGGATCCCGAAAAACCCCATCCTGCTATTCGGTTTTTCATCGCCCCACGGCCTGGCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAAt                                                                                                                                <  1:25556/236‑1 (MQ=255)
                                                                                                              aGCTGGATCCCGATAAACGCCATCGTGCTATGCGGTTTTTCATCGGCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATcgacg                                                                                                                           <  1:736084/238‑1 (MQ=255)
                                                                                                                       cccaTAAACCCCCTCGTGCTGTGGGGTTTTTCCTCGCCCCCCGGCCTGGCCCAGGCGGCGCGGTAGAGGCGCTGGACGCCCTGGAACAGCAGGGGGTTAGGTTCGCCGTCAAGCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGtt                                                                                                                <  2:975300/236‑1 (MQ=255)
                                                                                                                          aTAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATccg                                                                                                                                                                                                             <  1:255255/145‑1 (MQ=255)
                                                                                                                               ccccATCGTGCTATGCGGGTTTTCCTCGCCCCCCGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTAt                                                                                                        <  1:400550/237‑1 (MQ=255)
                                                                                                                                  cATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTg                                                                                                     <  1:863381/239‑1 (MQ=255)
                                                                                                                                   aTCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGATAGGTTCGCCGTCAATCCACAGTCTACCTTTGTAACGGCACAGTTTATCCGCCTAACACAGCAGCACGTTTTCCGTCACGCGGGAAACTTCGCCGATACATACCGGGAAACCCCGTTCCACCCCCATAGCCGACAAATCCtttttttttactcc                                                                                                    >  2:806098/1‑233 (MQ=255)
                                                                                                                                    tCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCCCCCGGGAAACTTCGCTGTTATCTACCGGGTAATCCCGTTCCACCCCAATCGACGCAAATTCGGTTTTTTTATCtcgt                                                                                                   >  1:356822/1‑236 (MQ=255)
                                                                                                                                     cGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGGACAGCAGGCGGGTAGGTTCGCCGTCAATCCCCATCCTCCCTTTTTAACGTTGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTTCCTGATATTCCACCGGAGATCCGCTTCCACCCCAAACGCCGAAAAAGCTTTTTTGTTTaatccga                                                                                                   >  2:402916/1‑233 (MQ=255)
                                                                                                                                     cGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGa                                                                                                  >  1:8837/1‑239 (MQ=255)
                                                                                                                                        gCTTTGGGGTTTTTCATCGCCCCAAGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGTTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATaa                                                                                               <  1:402916/239‑1 (MQ=255)
                                                                                                                                            tGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCCCAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGCCTCCAGCAGCAGGTTTTCCATACCCCGGGGAACTTCCCTGTTATCTACCGGGGAATCCAGTTCCCCCCCAATCGACGAAAAATCGTTTTGTTTTTTTGCGGAAAAggg                                                                                           >  2:975317/1‑237 (MQ=255)
                                                                                                                                            tGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGGAATCCAGTTCCACCACAATCGCCGAAAAATCGTTTTGTTTATTTGCGGTAAAATg                                                                                           >  1:729885/1‑239 (MQ=255)
                                                                                                                                                 tttttCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACACAAAATCGTTTTGTTTTATTGCGATAAAGTGGaaa                                                                                       >  1:370355/1‑238 (MQ=255)
                                                                                                                                                 tttttCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCCCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACAGAATATCGtt                                                                                                                >  1:868048/1‑213 (MQ=255)
                                                                                                                                                      cagcGCCCCACGGCCTGGCCCAGTCGGCGCTGGAAAGGCCCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAAGCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGt                                                                                 <  1:265141/236‑1 (MQ=255)
                                                                                                                                                       ctccccccACGGCCTGGCCCAGTCGGCCCTGTAGGGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGtt                                                                                <  2:356822/235‑1 (MQ=255)
                                                                                                                                                           ccccACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCCCAATCGACCAAAAATCGCTTTGTTTATCTGCGATAAAGTGGAAACCCGGTTTggg                                                                            >  1:479626/1‑238 (MQ=255)
                                                                                                                                                                   ccttgcccAGTCGGCGCTTTAGAGGCGCTGAACGCCCTGGAGCAGCAGGCGGTTAGGTCCGCCGTCAATCCACAGCATCCCGTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTGGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTgacg                                                                     <  1:848002/233‑1 (MQ=255)
                                                                                                                                                                    cggTCCCAGGCGGCGCTGTAGAGGCGCTGGACGCCCGGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTgacga                                                                    <  1:201592/236‑1 (MQ=255)
                                                                                                                                                                      gTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCCCAGCATCCCTTTTTAACGTAGCAGTTTATCCGCCGACCCCAGCAGCAGGTTTTCCATCCCCCGGGAAACTTCGCTGATATCTACCGGGGAATCCAGTTCCACCCCCATCAACGAAATAAAGCTTTGTTTATTTGCGATAAAGTGGAAACGCGGTTGGGGGcgggggaggt                                                                 >  2:744303/1‑230 (MQ=255)
                                                                                                                                                                      gTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGGAATCCAGGTACACCACAATCGACGAAATATCGCTTTGTTTATCTGCGTTAAAGTGGAAACGCGGTTTGGTGCTGACGACGt                                                                 >  1:955448/1‑239 (MQ=255)
                                                                                                                                                                      gTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGGTCGCCGTCAATCCACAGCATCCCTTTGTAACGGAGCAGTTTATCCGCCGACCCCCGCAGCAGGGTTTCCATCACGCGGGGAACTTACCTGATATCTAACGGGGAATACCGTTCCACCCCCATCCACGAAAAATCGTTTTGTTTTTCTGCGCATAAGCGGAAAACCGGTTTtgtggtcaccccgt                                                                 >  2:297763/1‑229 (MQ=255)
                                                                                                                                                                       tCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTTTTTGCGGTAAAGTGGAAACCCGGTTTGGTGCTGaccacggt                                                                >  1:426580/1‑237 (MQ=255)
                                                                                                                                                                              tggCGCTGTAGGGGCGCTGGACCCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCCCAGCACCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTttcttc                                                          <  2:426580/237‑1 (MQ=255)
                                                                                                                                                                              cGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTttcttc                                                          <  1:761337/238‑1 (MQ=255)
                                                                                                                                                                                 cgcTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGGAACTTCGCTGAAATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATAACGTTTTTTTTATCTGCGATAAAGTGGAAAACCGGTTTTGGGCTGGCGACGTTTTCTTCCAGc                                                      >  1:11409/1‑239 (MQ=255)
                                                                                                                                                                                 cgcTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTa                                                                                                                                                         >  1:607372/1‑141 (MQ=255)
                                                                                                                                                                                 cgcTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTa                                                                                                                                                         <  2:607372/141‑1 (MQ=255)
                                                                                                                                                                                            cccTGGCCGCCCTGGAACAGGGGGGGGTTAGGTTCGCCGTCAATCCCCAGCATCCCGTGGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAACCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  2:463773/236‑1 (MQ=255)
                                                                                                                                                                                            cccTGGCCGCCCGGGACAAGGAGGCGGGTAGGTTCGCCGTCAATCCCCAGCATCCCTTTGTAACGTAGCATTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGt                                            <  2:8837/236‑1 (MQ=255)
                                                                                                                                                                                              cTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGCCGAAATATCGTTTTGTTTATCTGCGTTAAAGGGGAAAAGCGGTTTGGTTCTTACGACGGTTTTTTCCAGCATAAAACCGtttg                                         >  1:839815/1‑237 (MQ=255)
                                                                                                                                                                                                ggACGCCCTGGAACAGCAGGCGGTTAGGGTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCACGAACAGGATTTCCATCACGCGGGGAAATTTGCTGATTTTTACCGGGGAATCCATTTCCACCACAAGCAGCGAAAAATCGTTTTGTTTTATTGCGATAAAGGGGGAAAGCGGGTTTGTGCGGTCGCCGTTTTTTTTCAGCATAAAAACGGTgggg                                       >  2:808981/1‑237 (MQ=255)
                                                                                                                                                                                                    gCCCTGGAACAGCAGGCGGTTAGGTTCGCCGGCAAACCACAGCACCCCTTTGTAACGTAGCAGTTTACCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTTATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTg                                    <  1:738942/238‑1 (MQ=255)
                                                                                                                                                                                                    cccccGGAACCGCAGGCGGTTAGGTTCCCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTg                                    <  1:1012217/237‑1 (MQ=255)
                                                                                                                                                                                                      ccTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTa                                                                                                                                                         >  1:317024/1‑120 (MQ=255)
                                                                                                                                                                                                      ccTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTa                                                                                                                                                         <  2:317024/120‑1 (MQ=255)
                                                                                                                                                                                                      ccTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAACCCGTGTGTGGTGAAc                                 >  1:11726/1‑239 (MQ=255)
                                                                                                                                                                                                        tGGAAAAGCAGGGGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcag                               <  1:744303/239‑1 (MQ=255)
                                                                                                                                                                                                         ggAAGAGCGGGCTGTTGGGTTCCCCGTCAATCCCCAGCACCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcagc                              <  2:527231/239‑1 (MQ=255)
                                                                                                                                                                                                         ggAACAGCAGGCGGTTAGGTTCGCCGTCAATCCAAAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAAcagc                              <  1:468765/239‑1 (MQ=255)
                                                                                                                                                                                                                cagGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTTCGATAAAGTGGAAACGCGGGTTGGGGCTGACGGCGTTTTCTTCCAGCCTAAAAACGTTGGTGTTGAACAGAAGGCCCa                       >  1:352464/1‑239 (MQ=255)
                                                                                                                                                                                                                cagGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGTTAAAGTGGAAACGCGGTTTGGGGCTGACGCCGTTTTTTTTCAGCATAAAACCGTTGGGTTTGaaaaaaaagccca                       >  2:311643/1‑228 (MQ=255)
                                                                                                                                                                                                                        tAGGTTCGCCGTCAATCCACAGCCTCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGAt                <  1:297763/238‑1 (MQ=255)
                                                                                                                                                                                                                             tccccGTCAATCCCCAGCCTCCCTTTTGAACGTAGCAGTTTATCCCCCTGCTCCAGCAGAAGGTTTTCCATCCCGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATAGCGTTTTGTGTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGcc          <  2:533443/236‑1 (MQ=255)
                                                                                                                                                                                                                             tccccGTCAACCCCTTGCATCCCTTTTGTACGGTAGCGTTTATCCGCCGACTCCAGCAGCAGGTTTGCCACCGCGCGGGAAACTTCGTTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGcc          <  2:167295/236‑1 (MQ=255)
                                                                                                                                                                                                                             tCGCCGTCAAACCAACGTCTCCCTTTGAAACGAAGCATTTTCTCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGc           <  1:413492/238‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTCCCCCGCCTCCCTTTTTAACTTTGCATTTTATCCTGCGACTCCAGCATGAGGTTTTCCATCACGCGGGAAACCTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAAGCGACGAAATATCGTTGTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGa  <  2:468454/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTCCACAGCCTCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGa  <  1:958926/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTCCACAGCATCCCTTTGTAACGTAGCATGTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGa  <  1:199057/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGa  <  1:311643/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGa  <  1:481320/239‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            
TATGGACTATTTTGAGTGAAATATCAGGCCGGATGCGGCTGCGCCTTATCCGGCCCATAACCCCTTACTTCCTCAACCCCGCAAACGCAGCCCGAATCTCTTCCTCCGGCAGCTGGATCCCGATAAACACCATCGTGCTATGCGGTTTTTCATCGCCCCACGGCCTGTCCCAGTCGGCGCTGTAGAGGCGCTGGACGCCCTGGAACAGCAGGCGGTTAGGTTCGCCGTCAATCCACAGCATCCCTTTGTAACGTAGCAGTTTATCCGCCGACTCCAGCAGCAGGTTTTCCATCACGCGGGAAACTTCGCTGATATCTACCGGGTAATCCAGTTCCACCACAATCGACGAAATATCGTTTTGTTTATCTGCGATAAAGTGGAAACGCGGTTTGGTGCTGACGACGTTTTCTTCCAGCATAAAACCGTTGGTGTTGAACAGCAGGCCCAGATCGATGTCGCCGTGGGTGA  >  NC_000913/4587845‑4588312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: