Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G96.8% 184.0 / ‑3.6 60intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (37/20);  total (39/21)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCC  >  NC_000913/3560218‑3560673
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             
aTCAAGGTGTTAGCACGGTTGATATTGTTGGTGACAATGCGCAATTTGCTGTGATGGAGCATTGCGTGCGCTACCGCTTCGGGCGTGGTGCCGATATCGATAACCGGCGTCGAGCCATTGGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcg                                                                                                                                                                                                                               <  1:716550/239‑2 (MQ=255)
  cAACGTGTTCGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGCTATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGGCTGGTTGGCCTTGCGATCGTGCCACAGCGCGTTAACGGAAATTGAAGGAGGCGCACCACCGCCATGATGGcgca                                                                                                                                                                                                                             >  2:10067/1‑236 (MQ=255)
           aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCAACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGAGTTAACCGACCTGCAAGGCAGCTCCGCCCCGCCTTGTTGGcccaggacaaggt                                                                                                                                                                                                                   >  1:617561/1‑228 (MQ=255)
                  ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGAATCAGGTTTTGCt                                                                                                                                                                                                            >  1:1179583/1‑238 (MQ=255)
                           ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                  >  1:17469/1‑239 (MQ=255)
                           ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTc                                                                                                                                                                                                  >  1:1218866/1‑239 (MQ=255)
                                     tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                        >  1:288508/1‑239 (MQ=255)
                                                cTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGGTAAACAGCGTCGAGCCATTGGTGATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAATGCAGCGCGGCACCCCCTTAATGGCCAAGGATCAGGGTTTTCTCCCCCAGCCACATGAGGCCGGGGCGacaatt                                                                                                                                                                             >  2:475927/1‑234 (MQ=255)
                                                   tgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGACCAGGGTTTGCTCCGCCAGCTCATTGAGGGCGCCGAGAAAAGtggg                                                                                                                                                                          >  2:823013/1‑236 (MQ=255)
                                                        gAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGCCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGCTTTGCTCCGCCAGGCCATTGAGGTCCCGGCGAATAGCCTGCggcc                                                                                                                                                                     >  1:698382/1‑237 (MQ=255)
                                                         aGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGcg                                                                                                                                                                    >  1:382664/1‑238 (MQ=255)
                                                               gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTCATGGCGCCGGAACCGGTTTTTCTCCCCCAGCCCCTTGAGGGCCCGGCGAAAAGTCTGCGGGGCGACggg                                                                                                                                                              >  2:9611/1‑238 (MQ=255)
                                                                cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTACGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                             >  1:692735/1‑239 (MQ=255)
                                                                       tACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTAGAGCCGTTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTATTTTTCTTCGGTCTGGGCGGCCTTGCGATCGTGCCACGCCGGGTTGACCGCACTGGCAGGCCGCGTCACACCGCCATCATGGCGTAGCATCCAGTTTTGATCGCCTCGCCCATTGAGGTCCGCGCAAACAGTCTGCCGGGCGACgggggaacat                                                                                                                                                       >  2:1204010/1‑230 (MQ=255)
                                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTGTTGCTCCACCAGCACATCGAGGTCGAACAGAACAGCATGCGGCCTCACGGAaaaagggcc                                                                                                                                                     >  2:289514/1‑234 (MQ=255)
                                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCTCCAGCTCATTGAGGTCGCGGCGAAAAGGCTGCGGGCGGAGGGAGAAATGct                                                                                                                                                      >  2:178175/1‑238 (MQ=255)
                                                                        aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAACAGGCTGCGGGATGCCGGGGAAATGcg                                                                                                                                                      >  2:929405/1‑237 (MQ=255)
                                                                              tCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCa                                                                                                                                                >  1:601369/1‑238 (MQ=255)
                                                                               ccGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAg                                                                                                                                               >  1:154260/1‑238 (MQ=255)
                                                                                         cccattatCGATAAACAGCGTCGAGCCTTGGGGGATTGGCCCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                     <  1:178175/233‑1 (MQ=255)
                                                                                             atatCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACGGAc                                                                                                                                >  1:402131/1‑239 (MQ=255)
                                                                                                  gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCCCTACCAGCCCTTCGGTACTGCCATaa                                                                                                                            >  1:538673/1‑238 (MQ=255)
                                                                                                       actgCGTCGTGCCATTCGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGAAAGCGCCTCACCGCCATGAGGGCGACGGATCAGGTTTTGCTCCGCCGGCTCATTGGGGTCGCGGCGAAGAGTCCGCGGGCTGCACGCGAAAGTGTCTACACGGGCTTCGGTACGAACATaaaacagc                                                                                                                      >  2:212398/4‑233 (MQ=255)
                                                                                                           cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                  >  1:512369/1‑239 (MQ=255)
                                                                                                           cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCGGtt                                                                                                                  >  1:372480/1‑239 (MQ=255)
                                                                                                             tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCTAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAACAGTCTGCGGGCGTACGGGGAAATGCTCTCCCCGCCCTTGGGCACTGACATAACCCGCCCGTTTa                                                                                                                >  2:929590/1‑239 (MQ=255)
                                                                                                             tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAAGGGCTCGGGGCTTGCGGGGGAATCCTACACCAGCCTTTCGGGGCTGCCACACCCCTGCTGTTTa                                                                                                                >  2:886258/1‑239 (MQ=255)
                                                                                                              cGAGCCTTTGGGGTTTGCCCCCGCCACTTTGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                <  1:823013/238‑1 (MQ=255)
                                                                                                                   atttGGGGTTTTGCCCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                          <  1:262897/237‑1 (MQ=255)
                                                                                                                    ttgggtttttttCTCCTCCCCTTTGGGGGCTGTGGCCTTTTTTTCTTGTGGCGGGGTGGCCTGGCACTCGTGCCAGGGGGTTTTAACCAAAGTGGAGGGCAGCCCCGCACCGCCATGATGGCGTAGGATGAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                         <  2:538140/231‑1 (MQ=255)
                                                                                                                    gttGGGGTTTTCCCCCGCCATTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCAGGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                         <  1:759847/238‑1 (MQ=255)
                                                                                                                     ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCCGCGCCGCACCGCCATGTTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGTTCGCGGCGAATAGCGTGCGGGCTGACGGAGAAATGCTTTAACAGCCCTTCGGTACTGCCTTAACCCCGCGGtttcgccatcac                                                                                                        >  2:262897/1‑230 (MQ=255)
                                                                                                                     ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCTTCGGAACTGACATAACCCTGCTGTTTAACAAGTTc                                                                                                        >  1:538140/1‑239 (MQ=255)
                                                                                                                     ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCAGTTc                                                                                                        >  1:1293575/1‑239 (MQ=255)
                                                                                                                           aTTTGCCCCGCCACTTTGCGGGCGATGCGCTTTTTTCCTTCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                                   <  1:1139600/238‑1 (MQ=255)
                                                                                                                            tttGCCCCGCCCTTTTGCGGGCGATGCGCTTTTTTTCTGCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                 <  1:164521/239‑1 (MQ=255)
                                                                                                                               gccccGCCCTTTTGGGGGCGATGCGCTCTTTTTCCTCGGTCTGGGTGGCCTTGCGATCGGGCCCCGGGGTGTTACCCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg                                                                                              <  1:252667/236‑1 (MQ=255)
                                                                                                                               gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGACGCGGCGACTAGTCTGCGGGCTGACGGCGAAATGCTCTACCAACTATTCGGTACTGACATAAACCTTCTGTTTAACCAGTTAGATAATAACg                                                                                              >  2:244871/1‑239 (MQ=255)
                                                                                                                                cTCCGCCACTTTGCGGGCCAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGTCTCAGCCAGCTCATTGAGGGCGCAGAGAAAAGTCTCCGGGGGGACGGAGAAATGCTCAACCTGTCCGTAGGTATTAACTAAACCCTGCTGTTTAAAAAGTTTAATAAAACCGt                                                                                             >  2:263506/1‑239 (MQ=255)
                                                                                                                                    cccccTTTGGGGGGGATGCGCTTTTTTTTTTGGGTTGGGGTGGCCTTGGGATCGGGCCAGGGGGTTTTAACCGAACTGGAAGGCAGGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                         <  1:9611/235‑1 (MQ=255)
                                                                                                                                                   ttGCGCTTTTTTGCTCGGGTTGGGGGGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACGGGAAGGCGGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                          <  1:475927/237‑1 (MQ=255)
                                                                                                                                                    tGCGGTCTTTTTCTCGGGTCGGGTTGCCCTGGCGATCGTGCCACGGGGTGTCAACCGACTGGGAAGGCAGCGCCGGACCGGCATGATGGCTCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                        <  1:273168/239‑1 (MQ=255)
                                                                                                                                                     gcgcTTTTTTTCTGCGGTCTGGGTGGCATGGCGATCGGGCAACGGCGTTTTAACCAACCTGGAATGAAGCGCGCCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCAGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                       <  1:1137506/239‑1 (MQ=255)
                                                                                                                                                            ttttCTTGGGCTGGGGTGGCCTTGGGTGCGGGCCCGGGTGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaa                                                                <  1:886258/239‑1 (MQ=255)
                                                                                                                                                                 tgcggtggtggTGGCCTGGCGCTCGGCGCTGGGCGTGTTGACCGCACCGGAAGGCGCCGCGGCACCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc                                                           <  2:17469/230‑1 (MQ=255)
                                                                                                                                                                     gTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                              <  1:328899/185‑1 (MQ=255)
                                                                                                                                                                     gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGa                                                       >  1:299153/1‑239 (MQ=255)
                                                                                                                                                                               ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGTTGACGGAGAAATGCTCTACCAGCCCTTCGGTCCGGACATAACCCTGCTGGTTAACCAGTTCAata                                                                                                    >  2:328899/1‑185 (MQ=255)
                                                                                                                                                                               ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCCGCTCTTCGGGCCTGACTAAACCCTGCGGTTTCACCAGTTCGATAATCCCTTTTTGCCTCTGtgtttgt                                                                           >  2:140054/1‑209 (MQ=255)
                                                                                                                                                                                  tGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATACAACCGTTTGGGCGTTGTGTTTGTTTCATTTTTAAATCACCGGAATTTtttttgttt                                          >  2:1262506/1‑239 (MQ=255)
                                                                                                                                                                                    cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGGGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                        >  1:903620/1‑239 (MQ=255)
                                                                                                                                                                                         gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCCTTTTTAAATCCCTGGAATTATTTTCGTTTTCGCGCa                                   >  1:888663/1‑239 (MQ=255)
                                                                                                                                                                                              aCGGCGTTTTACCCGAACGGGAAGGGCGCGCTGGACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                              <  1:1111124/239‑1 (MQ=255)
                                                                                                                                                                                                  gtggtaaaCCGAACGGGAAGGCAGGCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGa                           <  1:929590/232‑1 (MQ=255)
                                                                                                                                                                                                    tgtaacccGAACGGGAAGGCAGCCCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                         <  1:805159/232‑1 (MQ=255)
                                                                                                                                                                                                    tgtTAACCGAACTGGAAGGCAGCGACGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAACAGTATGCGGGCGGCCGGAGAAATCAACTACCAGCTATTCGGGACTGACTGAACCATGCTGTTTAACCACTTCGATAAAACCTTTTGACCATTGCGTTGGTGTCATTTTTAAAACCATGGACTAATTTTAGTTTTCCCGCAtgaagtgatta                        >  2:131492/1‑229 (MQ=255)
                                                                                                                                                                                                    gtttAACCGAACTGGAAGGCAGCGCCCGCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                         <  1:156276/236‑1 (MQ=255)
                                                                                                                                                                                                              acgggAAGGCACGCCCCCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc              <  1:405315/236‑1 (MQ=255)
                                                                                                                                                                                                              aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc                             >  1:302410/1‑224 (MQ=255)
                                                                                                                                                                                                              aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc              >  1:1186387/1‑239 (MQ=255)
                                                                                                                                                                                                                          cgccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTGTTTAACCAGTTCGATAATACCGATGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGTGCGAATCAACAAAATCCATCGCTAAAcac  >  1:299936/1‑237 (MQ=255)
                                                                                                                                                                                                                          cgccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                              >  1:149337/1‑179 (MQ=255)
                                                                                                                                                                                                                          cgccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                              <  2:149337/179‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             
ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCC  >  NC_000913/3560218‑3560673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: