Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,274,349 T→G 24.7% G57G (GGT→GGG yejB → microcin C ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,3490TG24.7% 90.3 / 10.4 60G57G (GGT→GGGyejBmicrocin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (11/32);  new base G (14/3);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 9.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGT  >  NC_000913/2274113‑2274582
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           
gATACCGGGTGGTATGACGGCAATAAAGCGGCCAACCTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGgtgt                                                                                                                                                                                                                                           <  1:1232426/239‑1 (MQ=255)
 ttcccTGGTGGTTTGACGTCAATAAAGCGGCCAAACGGCCGTCCGCCAGCAAAAAGGGAGAGTAGATGGGCGTTTACCTGATTCCCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGtt                                                                                                                                                                                                                                          <  2:334957/236‑1 (MQ=255)
 ctccttggtggTAACCCGACTATAAGGCGGCAAAACTGCCTGCCGCCGGCAAACGGGGAGAGTAGATGGGTGCTTCCCGGATTCGCCTTGTGTTGCTGGGGATCCCAACATTAGGGGGGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGtt                                                                                                                                                                                                                                          <  2:422122/234‑1 (MQ=255)
      tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCCGGCGGGGAAGGGGtttggt                                                                                                                                                                                                                                      >  1:1169391/1‑234 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGTTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGCGCAGGTGTTccggacgccca                                                                                                                                                                                                                               >  1:460102/1‑229 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGTgccagc                                                                                                                                                                                                                                 >  1:1020012/1‑237 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGCGGGGGCAGGGGTTACTgcccgcc                                                                                                                                                                                                                                >  1:209327/1‑238 (MQ=255)
            tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGCCGACAAGGCCATCGCCGCCATTGAGGTTGCTAATGCCGGGGGATTACCCGGCACAGGCGGGGGAGGGGTTCGTgccgtcc                                                                                                                                                                                                                                >  2:580067/1‑234 (MQ=255)
             aTGGCGTCAAAAAAGCGCACAAGCGCCCTGCCGCCGGAAAAGAGGGAGAGTCAATGGGCGCTTCCCTGATTCCCCTTCTGTTGCGGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCACCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCAt                                                                                                                                                                                                                              <  2:946722/239‑1 (MQ=255)
                 cGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGCGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATAGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGTGCCAGCCAtcggc                                                                                                                                                                                                                          >  1:45371/1‑235 (MQ=255)
                  gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTTAAGGGGTTCGTGCCAGCCATGCGCa                                                                                                                                                                                                                         >  1:584185/1‑239 (MQ=255)
                      aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCCAACg                                                                                                                                                                                                                     >  1:1169437/1‑239 (MQ=255)
                          aggggCCAAACTGCGGGCCGGCAGCAAACAGGGAGGTTAAATGGGCGTTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGgtg                                                                                                                                                                                                                 <  1:94425/236‑1 (MQ=255)
                            cGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGCCGGTGAAGGGGTTCGTGGCGGCCATGGCCAAACGGgggtg                                                                                                                                                                                                               >  1:1245350/1‑238 (MQ=255)
                            cGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCGGGCGGTCCGGTCGACCAGGCCATCCCCGCCATTGGTTTTGGTAGTGCCGGAGTATTACCCGGCGCAGGGGGTGAAAGGGGTCGTTCAACCCATGGGAAAACggagggc                                                                                                                                                                                                               >  2:1104920/1‑234 (MQ=255)
                                         gTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCCGCCATGCGCAAACGGGGGTCgcgcaaataagc                                                                                                                                                                                                   >  1:829707/1‑227 (MQ=255)
                                            agCCAGCAAAAAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                                <  1:752061/237‑1 (MQ=255)
                                                             agTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCCAACGGGGGTCGGCCATATCAGCGACAGCAATTACCCGGGCgg                                                                                                                                                                             >  1:661967/1‑238 (MQ=255)
                                                                aGATGGGCTCTTCCCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                          <  1:1264007/238‑1 (MQ=255)
                                                                         cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGa                                                                                                                                                                 >  1:1059464/1‑238 (MQ=255)
                                                                         cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGGGCCAACGGGGTTCGGCAATATCAGCGACAGGAATTACCCGGGCGGACCCGGAtttaa                                                                                                                                                                 >  1:1177468/1‑235 (MQ=255)
                                                                          tttccTGATTCGCCGTCTGTGGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTTCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATc                                                                                                                                                               <  1:1150425/236‑1 (MQ=255)
                                                                            aCCAGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCa                                                                                                                                                             <  1:242590/239‑1 (MQ=255)
                                                                                             ttGCTGGTGATCCCAACATTATGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCt                                                                                                                                                 <  1:662047/234‑1 (MQ=255)
                                                                                                  ggTGATCCCAACATTAGGGGCGATTAACACCATCAACTTTTTCATCATGCAAATTGCGCCTGGCGGTTCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACt                                                                                                                                        <  1:1296612/238‑1 (MQ=255)
                                                                                                   gTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCCTCGCCGCCATTGAGTTTGGTAATGCCGGAGCATTACCCGGCGCAGGCGGCGACGGTGGTCGTGCCAGCCATGAGCAAAACGGGGTCGGGACTATCAACGACAGTAATATACCCGGCGGACGCGGGGCAGAACCCAAAGTGATCCCGgaggagacta                                                                                                                                       >  2:1155148/1‑231 (MQ=255)
                                                                                                    tgtcccaactgttATGGGCGATTATCACATACCAATTTTTCATCGTGCAAAGTGCGCCGGGCGGCCGGGTCGACCGGGCCATCGCCGCCAGTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTTCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGTCAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCAt                                                                                                                                     <  2:19175/228‑1 (MQ=255)
                                                                                                       tCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGGGCCAGCCCAGCGCAAACGGGGGGCGGCAATATCAGCGGCCGTAATTAACCGGGGGGGCCGGGGTTATATCCAAAAAGGATCGGCGAAATCACTCaccat                                                                                                                                  >  2:662047/1‑235 (MQ=255)
                                                                                                         ccAACATTTTGGGGTATTACCCCCATCAACTTTTTCAGGTTGAAAATGGCGCCTGGGGGCCCGGTCGCCCAGGCCTTGGCCGCCATTGAGTTTGTTAATGCCGGAGTATTTCCCGGCGCAGGCGGTGAAGGTGTTCGTGCAAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTa                                                                                                                                <  2:1245350/239‑1 (MQ=255)
                                                                                                                  tGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACCGTAATTACCGTGGCGGACGCGGGTTAGATCCCGAAGTGATCCCTGAGATCACCCACCGCTACGGGTTTgt                                                                                                                       >  1:1198639/1‑238 (MQ=255)
                                                                                                                    ggCCATTACCACCATCAACTTTTTCATCGGGCAAAGTGCGCCGGGGGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGAt                                                                                                                      <  1:481173/238‑1 (MQ=255)
                                                                                                                        aTTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGGTTAGATCCAGAAGGGATCGCTTGGATCACTCATCGCCCCGGGTTTGGTAAAcc                                                                                                                 >  1:546968/1‑239 (MQ=255)
                                                                                                                         ttactcccATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                                <  1:1104920/233‑1 (MQ=255)
                                                                                                                          tATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGa                                                                                                               <  1:1024348/239‑1 (MQ=255)
                                                                                                                                taTCAATTTTTTCATGTTGCAAATTGCGCCTGGGGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGTGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACg                                                                                                         <  1:319790/238‑1 (MQ=255)
                                                                                                                                cATCAATTTTTTCATCGTGCAAATTGCGCCGGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACg                                                                                                         <  1:478103/239‑1 (MQ=255)
                                                                                                                                         tttCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGGGATCGCTGAGATAACTCATCGCTACGGGTTTGATAAGCCGATCCACGCACGTTACt                                                                                                >  1:879276/1‑239 (MQ=255)
                                                                                                                                               tctGCACATTGTGCGCTGGCTGCCCGCTGGCCCAGGCTACGGCCTCCATTGGTTTCGGTAACGCAGGAGTTTTGCAGGGCGCCGGCGGTGACGGTGTTCGTGCCACCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTATCGTGGCGGACGAGGATTAGATCAAGAAGTGCACGCTGAGATCACTAATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTATGTCaaa                                                                                           <  2:1246216/237‑1 (MQ=255)
                                                                                                                                                 tGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCACACCATGCGCAAACGGGTGGCGGCCATACAAGCGACAGTAATTACAGCGGCGGACGCGGGGTAGATCCCGAAGGGGTCGCGGAGGAGACTACACCCTACGGTGTTGATACCGCCATCCACCGACGTTCACTCAAAAt                                                                                         >  2:4266/1‑238 (MQ=255)
                                                                                                                                                                     tccggtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCt                                                                                                                                 >  1:396903/1‑179 (MQ=255)
                                                                                                                                                                     tccggtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCt                                                                                                                                 <  2:396903/179‑1 (MQ=255)
                                                                                                                                                                      ccggtcGACCAGGGCATCGCCGCCATTGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTCCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCt                                                                     <  1:1222713/238‑1 (MQ=255)
                                                                                                                                                                         gtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTTGATCCAGAAGTGATCGCTGCGATCACTCATCGCTACGGGTTTGTTAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCCCTTTGa                                                                 >  1:744955/1‑239 (MQ=255)
                                                                                                                                                                            gACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGAtt                                                               <  1:574287/238‑1 (MQ=255)
                                                                                                                                                                                 cgCCTTCCCCGCTTTTTAGTTTTTTTATGCCGGGGTTTTACCCGGGGGCGGCCGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCg                                                         <  2:584185/238‑1 (MQ=255)
                                                                                                                                                                                   tcctgggcgccATTGGGTTTTGGAAGGCCGGAGTATTACCCGGCGCGGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGGCGGCAATATCATCGACAGTAATTTCCGGGGCGGAAGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGa                                                        <  2:1198639/231‑1 (MQ=255)
                                                                                                                                                                                   ccATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGa                                                        <  1:351342/238‑1 (MQ=255)
                                                                                                                                                                                      tCGCCGGCAGTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGc                                                    <  1:290063/239‑1 (MQ=255)
                                                                                                                                                                                            ccATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGGGATCGCTGGGATCACTCCTCGCTACGGTTTTGGTAAGCCGATCCACGGACGTTACCTCCAAATGCTCTGGGACTACATCCGCTTTGATTTTTGCGATAGCCTGtt                                               >  1:381433/1‑238 (MQ=255)
                                                                                                                                                                                                     tttggattGCCGGTGTATTTCCCGTCGCGTGGGTGTAAGGTTTGTGTGGCAGCCATGCGCAAACGGGTGTCGGCAATATCAACTACAGTAAGTGCCGTGGCGGAAGAGGATCAGATCCTGAAGTTATATGTGAGATCTTTCATGGCTACTGTTTTGATAAGCCGAGCCACGAACGTTACTTCAAAATGCTCTGGGACTACGTCCGCTTTGATTTTGGCGATGGCTTGTTTCGCGGCGcc                                     <  2:460102/232‑1 (MQ=255)
                                                                                                                                                                                                       ggTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCCAAAAGCTCTGGGCATACAACCGCTTTGATTTTGGCGATAACCTGTTTCGCAACGCCTc                                   >  1:540335/1‑239 (MQ=255)
                                                                                                                                                                                                                   tattttcccGGGGCAGGCTGTCACGGGTTTAGGGCCAGCCCTTGACAAACGGGTGCCTGCAATAGCAGCGACAGGAATGTCCGTGGCGGACTGGTATTAGAGCCAGAAGTGATCGCTGAGATCACTCATCGGTACGGTTTTGATAAGCCTATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  2:1110136/232‑1 (MQ=255)
                                                                                                                                                                                                                   gTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  1:305940/238‑1 (MQ=255)
                                                                                                                                                                                                                   gTATTACCCGGCGCAGGCGGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTAGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  1:709411/238‑1 (MQ=255)
                                                                                                                                                                                                                   aaaTTACCCGGGGCGGGCCGTGAAGGTGATCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  1:365042/236‑1 (MQ=255)
                                                                                                                                                                                                                    tATTCCCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                        <  1:1220779/237‑1 (MQ=255)
                                                                                                                                                                                                                    tATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGct                                                                                      <  1:1013785/175‑1 (MQ=255)
                                                                                                                                                                                                                          ccGGCTCAGGCGGTGAAGGTGGTCGGGCCCGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGCAGCGGATTAGATCCAGAAGTTATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTaaa                <  2:1059464/239‑1 (MQ=255)
                                                                                                                                                                                                                              cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGGCAGGAATTACCGGGGGGGACGCGGCTTTGATCCACAAGTGATCGCTGGGAACACTCCTCGCGCACGTTTTGGTAAAGCGCACCCCGAACGTTACTTCCAAAAGCCCCGGGCGCACATCCGCTTTGATTTTTGCGATAGCCCTTTTCTCAGCGCCCCGGGGCGCACGCCGTTTTAAAACa            >  2:10282/1‑239 (MQ=255)
                                                                                                                                                                                                                                  cggggggagaGGGGTTCGGGCGACACATTCGCAACCGGGGGTGGGCAATATAAGCGACAGTAATTACCGGGGGGGAGGCGGATGAGATCCAGAAGTGATCGGTGAGACCACACATCGCTACGGTTTTGATAAGCCGATCCACGAACGGTACTTGAAAATGCTCTGGGACTACATCCGATTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGAAAGt          <  2:1169391/229‑1 (MQ=255)
                                                                                                                                                                                                                                          aaGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGt  <  1:1155148/238‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           
GATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGT  >  NC_000913/2274113‑2274582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: