Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,950,173	A→G	18.6%	E311G (GAG→GGG)	argA →	amino acid N‑acetyltransferase and inactive acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,950,173	0	A	G	18.6%	121.9 / 2.7	50	E311G (GAG→GGG)	argA	amino acid N‑acetyltransferase and inactive acetylglutamate kinase
Reads supporting (aligned to +/- strand):  ref base A (2/38);  new base G (7/3);  total (9/41)
Fisher's exact test for biased strand distribution p-value = 3.81e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGG  >  NC_000913/2949940‑2950406                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                ggTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGGATTTTGGGGTTgt                                                                                                                                                                                                                                            >  1:96078/1‑238 (MQ=255)   aaaaaGCCCCGGAAGGGGAAGGCGATTACAACTCCGGTACGGTGCGCTTTGTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGGTGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGGGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGAt                                                                                                                                                                                                                                           <  1:832942/235‑1 (MQ=255)      aaGCCCAGGAAGAGAAAGGCGATTACAACCCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGc                                                                                                                                                                                                                                       <  1:364835/239‑1 (MQ=255)       atcccGGGTAGAGAAAGGCGATTACCACGCCGGTATGGTGCGCTTTTTGCGTGGCCCAGTGAAAGCCTGCCGCAGCGGCGTGCGGCGATGTTATTTAATCAGTTACCAGGAAGATGGCGTGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGcc                                                                                                                                                                                                                                      <  2:316794/237‑1 (MQ=255)       aGCCCGGGAAGAGAAAGGCGTGTCCACCGCCGGTCCGGGGCGCTGTGTGGGTCGCGAAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTTCGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGATTTGATTCGcc                                                                                                                                                                                                                                      <  2:1156613/239‑1 (MQ=255)        gCCCAGGAAGAGAAAGGGGTTTACCCCCCCGGTTCGGTGCGTTTTTTGCGTGGCCCAGTGAAAGCCTGGCGCAGCGGCGTGCGTCGCTGTCATTTAAGCAGGTAGCAGGAAGATGGCGCGCTGTGGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGcc                                                                                                                                                                                                                                      <  2:498364/238‑1 (MQ=255)                  agaAAGGGCGTTGCAACCCCGGTACGGTGGTCTTTTTGCGGTGGGGAGGGAAAGCCTGGCGCAGGGGCGTGGGGCGCTGGCATTTAATCAGTTGTCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGagca                                                                                                                                                                                                                            <  2:1261064/238‑1 (MQ=255)                    aaaGGCGATTACAACTCCGGTACGGTTCCCTTTCTGGGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcagca                                                                                                                                                                                                                         <  1:865964/239‑1 (MQ=255)                    aaaGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGCTATTGGCGGTATTTTGGGGTTTGTTCGCCCCCTGTagacgcc                                                                                                                                                                                                                         >  1:368809/1‑234 (MQ=255)                     aaGGGGATTCCAACTCCGGTACGGTGCGGTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcagca                                                                                                                                                                                                                         <  1:1136547/238‑1 (MQ=255)                     aaGGCGATTCAACCTCCGTTAGGGGGCGTTTTTTGGGTGGCGCGGGGAAAGCCTGCCGCACGGGGGTGCGTCCCTGTCATTTAAGCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTAGCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcagca                                                                                                                                                                                                                         <  2:546421/238‑1 (MQ=255)                     aaGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcagca                                                                                                                                                                                                                         <  1:878838/238‑1 (MQ=255)                         cggttgcagttCCGGTACGTTGCGTTTTTTGCGGGGCGCGGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGGCATTTAATCAGTTATCAGGAATATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAgg                                                                                                                                                                                                                      <  2:96078/227‑1 (MQ=255)                           aTTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGGATTCTGGGGTTTGGTTGCCCACCTGGGCAGCAAggggtt                                                                                                                                                                                                                  >  1:643421/1‑235 (MQ=255)                                             tGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGGGGTTATTTGCCCCACGGCGGAGCAAGGTATTCTGGTATGCCCtttt                                                                                                                                                                                                   >  1:1042750/1‑234 (MQ=255)                                                     ttGCGTGGCGCAGTGAAAGCCTGCCGCAGGGGCGTGCGTCCGTGTCATTTAAGCAGTTATCAGGAAGGTGGCGCGCGGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCt                                                                                                                                                                                        <  1:692531/239‑1 (MQ=255)                                                      tGCGGGGCGCAGTGAAAGCCGGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTg                                                                                                                                                                                       <  1:584268/239‑1 (MQ=255)                                                        cGTGGCGTAGTGAAACCCTG‑‑GCAGCGCGGTGCGTCGCTGTCATTTAAGTGGTTAGCTGTAATATGGCGCGCTGTTGCAAGATTTGCTCCCACGCGACGGTATCGGTACACAGATTGTGATGGAAAGCGCCGATCAGATTCGTCCCGCAACAATCAATGATATTGGCGGTATTCTCGAGTTGATTCGCGCTCTGGAGCAGCAAGGTACTCTGGTACGCCGTTCTCGCGAGCAGCTGgaga                                                                                                                                                                                   <  2:19445/239‑1 (MQ=255)                                                                          tGCCGCAGGGGCGTGCGTCGCTGGCATTTAATCAGTTAACAGGAAGATGGCGCGCTGTTGCAAGAGTTGGTCTCACGCGACGGTAGCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAAtt                                                                                                                                                                   <  1:276355/239‑1 (MQ=255)                                                                          tGCCGCAGCGGGCTGCGTCGCTGTCATTTAATCAGTTACCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAAtt                                                                                                                                                                   <  1:1079236/239‑1 (MQ=255)                                                                            ccGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGCATTCTTGGGTTGATTCTGCCACCGGAGCAGCAAGGTATTCtt                                                                                                                                                                                                               >  1:42418/1‑192 (MQ=255)                                                                              gCAGTGGCATGCGTCGCTGGCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGGGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCAcc                                                                                                                                                               <  1:915692/239‑1 (MQ=255)                                                                                           tCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAgcgc                                                                                                                                                  <  1:388219/238‑1 (MQ=255)                                                                                                             ttATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTTGGGTTGATTTGCCCACCTGCGCCGCCAGGTATTCTGGTACGCCGTTCTCCCGCGCAGCTGGGGATTGGAATCGACAAATTTACCCTTA‑TTTAGCGCGATAAAACGAC‑TATTTcc                                                                                                                               >  1:1018675/1‑238 (MQ=255)                                                                                                                  aGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGcgcc                                                                                                                        <  1:493471/239‑1 (MQ=255)                                                                                                                    gAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAaca                                                                                                                                           <  1:895469/220‑1 (MQ=255)                                                                                                                     agtgtGGCGCTCTGTTGTAAGCGTTTTTCTCACGGGACGGGCTCGGTAGGCTGATTGTGATGGAAAGCGCCGTTCAGTTGCGTCGAGCAATAATCAACGGTAGTGGCCGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACTCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCTCCACTC‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGcgcc                                                                                                                        <  2:623348/232‑1 (MQ=255)                                                                                                                              cgcTGTTGCAAGGTTTGTTTTCACGGGCCGGTATCGGTTAGCGAGTTGGGAAGGAAAGGCCCGGGCAGGTTCGTCGCGGAACAAGCAACGATGATGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGGAGCAATGTATGCTGGTTCGCCGTTCGCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGACGCGCTCTATcc                                                                                                             <  2:550897/238‑1 (MQ=255)                                                                                                                                    tGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCg                                                                                                    <  1:1105660/239‑1 (MQ=255)                                                                                                                                           gttgttCTCACGCGACGGTATCGGTACGCAGATTGGGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGgaagag                                                                                              <  1:596445/238‑1 (MQ=255)                                                                                                                                           gttggtCCCCCGCGCCGGTATCGGTACGCAGATTGTGAAGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGgaagag                                                                                              <  1:762097/238‑1 (MQ=255)                                                                                                                                            ttgttCTCACGCGGCGGTATCGGTACGCAGATTGGGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGgaagaga                                                                                             <  1:564500/238‑1 (MQ=255)                                                                                                                                               ttCTCACACGCCGGTTTCGGTACGCAGATTGTGTTGGAACTCGCCGATCAGTTTCGTCGTGCAACAAACAACGGTATGTGCGGTAGTCTGGAGTGGAGTAGCTGACTGGAGCAGAAAGGTATTTTGGTTCGCCGTTCTCGCGAGCAGCTTGAGATTGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑GTCCCGGAAGAGAAGA‑t                                                                                        <  2:592027/239‑1 (MQ=255)                                                                                                                                                 ctcACGCGCCGGTTTCGGTACGTAGATTTGGATGGAAAGCGCCGAGCAGGTTCGTCGCGGAACAATCAACGATATTGGCCGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTg                                                                                      <  1:554754/239‑1 (MQ=255)                                                                                                                                                                  gTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGcgccg                                                                                                                       <  1:1000315/192‑1 (MQ=255)                                                                                                                                                                     cGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGGATTGTTGAGGTTGTTTGGCCACCGGGGCAGCAAGGGATTCTGTGGTACCGTTCTCGCCGGCAGCCGGAGACGGATATTGCACAATTCACCATCATTTTAGC‑CGCAAACAGGAC‑CATTTCCTTGCGCGCCGC‑CTATCC‑GCTTCCCGG‑AGAGAAGACTGGGGGAAATTCCCTGTggggg                                                                   >  2:982817/1‑238 (MQ=255)                                                                                                                                                                          attggtaTGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTg                                                                                      <  1:242047/208‑1 (MQ=255)                                                                                                                                                                                    aaaGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGGTTTTTGGGGGTGATTCGCCCACCGGGGCAGCAAGGGATTTTGGTACACCGTTCTCGCGAGCAGCCGTGGATGGGAAATGACCAATTTACCACTA‑TTTATCGCGGTAAAAAGGA‑CATTTCC‑TGCGCCCGCCTCCATCCTG‑TGCCCGAAGAAAAACA‑TTTGGGAAAAGCCCTGGGGGGGGGTTCCCCCgagaa                                                     >  2:452476/1‑234 (MQ=255)                                                                                                                                                                                      agggcCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCg                                                 <  1:1053250/236‑1 (MQ=255)                                                                                                                                                                                       gcgcCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGATTTGATTCGCACACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATTGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCg                                                 <  1:1134883/238‑1 (MQ=255)                                                                                                                                                                                           cgcgCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGt                                             <  1:1006467/235‑1 (MQ=255)                                                                                                                                                                                                   ttCGTCGCGAAACAATCAACGATATTGGCGGTATTCTGGGGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAg                                     <  1:550765/238‑1 (MQ=255)                                                                                                                                                                                                       tcgCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAac                                                                                                                                            <  1:569488/136‑1 (MQ=255)                                                                                                                                                                                                        cgcgCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGGGTGa                               <  1:296803/239‑1 (MQ=255)                                                                                                                                                                                                                 aTCAACGATATTGGCGGTATTCTGGAGTTGAGTCGGCCACCGGAGCAGCAAGGGATTCCGGGACCCCCTTCTCGCCGGCCGCTGGGGGGGGGAATCAGAAAATTTCCCACTA‑TTCAGCGCGGGCACACGACATTTTCCC‑TGC‑CCGCCCCCCACTC‑G‑ATCCCGGCAG‑GCAGAGTTGGTGGAAAGGCCCGGCTGGCCGTTTACCCGCATTTACGCAGTTCCTCAAgcgggaagagtcg                         >  2:817391/1‑227 (MQ=255)                                                                                                                                                                                                                  tCAACGATTTTGGCGGTAGTCTGGAGTTGAGTCGCCCACTGGAACAGCAAGGTAGTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGTGTGAAGTTctgctg                     <  1:958346/239‑1 (MQ=255)                                                                                                                                                                                                                         tatTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTa                                                                                                                                                                                                                    <  2:562753/47‑1 (MQ=255)                                                                                                                                                                                                                                gggaTTCTGGGGTTGATTCGCCCACTGGAGCAGCAAGGTATTGTGGTACGCCGTTCTCGCGAGCAACTGGAGATGGAAATCGACAAATTC‑CCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGc       <  1:791013/235‑1 (MQ=255)                                                                                                                                                                                                                                     tCTGGGGTTGATTCCCCCAGTGGAGCAGCAAGGTATTCCGTTACGCCGTTCTCGCAAGCTGCTGGTGATGGAAAACCACAAATTCACCATTA‑TGCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAgg  <  1:1253614/239‑1 (MQ=255)                                                                                                                                                                                                                                     tCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAgg  <  1:753477/239‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                GGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTA‑TTCAGCGCGATAACACGAC‑TATTGCC‑TGCGCCGCGCTCTATCC‑G‑TTCCCGGAAGAGAAGA‑TTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGG  >  NC_000913/2949940‑2950406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑