Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,277,500 T→G 17.2% V55G (GTG→GGG)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,5000TG17.2% 139.8 / 6.2 77V55G (GTG→GGG) yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (14/45);  new base G (10/4);  total (28/49)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT  >  NC_000913/3277266‑3277736
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                
attcaaaaAGGAAAGAACTTTGTCGCTGGCATGGACTTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGAtt                                                                                                                                                                                                                                              <  1:73937/239‑1 (MQ=255)
 ttaaaaaaGGAAAGAACCTTGCCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTCCCAAAAAGGTTTAATGGTTGGGCGCTATATGCCCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTg                                                                                                                                                                                                                                             <  1:1132082/233‑1 (MQ=255)
           aaagaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGACAATTATCAGAGAAAAGCCGCCCCAAAGTTATTGGCGGGTGTCCATAAAAGGAATTAGGAGCata                                                                                                                                                                                                                                    >  1:456766/1‑238 (MQ=255)
            aagaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAATCCCAAAAATGATTGAGGAGCgtaat                                                                                                                                                                                                                                  >  1:822325/1‑237 (MQ=255)
              gaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAATGAGTGAGGGGCATATCAc                                                                                                                                                                                                                                >  1:800621/1‑239 (MQ=255)
                   tgggcgcgGGCGTGGACTTCAAAATTGAGGATTGTATTGGCGTCAAGGAATAAGGGATTTCCCAAAAAGGGTTAAGGTTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGTCGTGTTAGTTGCCGAAGTCGAGATATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCa                                                                                                                                                                                                                           <  1:527812/235‑1 (MQ=255)
                                      cAAAATTGATGATGAGATTGGCGACGACGAATAATGGATTTCCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCAt                                                                                                                                                                                                         <  1:801600/238‑1 (MQ=255)
                                            tgatgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCCTAAATGGGTTGAGGAGCATATCACGGTAAATCCATCATCACCGGCATTCCGTCa                                                                                                                                                                                                  >  1:107703/1‑239 (MQ=255)
                                            tgatgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGGGATTGAGGGGCATATCACGGTCAATCCATACTCACCCGCATTACCTCa                                                                                                                                                                                                  >  1:342468/1‑239 (MQ=255)
                                             gatgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTAATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGAAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAt                                                                                                                                                                                                 <  1:1227549/239‑1 (MQ=255)
                                                 atgaGATTGGCGACGACGAATAATGTATTTACCAAAAGGGGTTAATGGTTGGGCGATATATGCTCACCCTTGTTTTCAGGAAACCTGCGACGCTTTAGTGGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAATTTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCa                                                                                                                                                                                             <  1:920991/239‑1 (MQ=255)
                                                  tgaGATTGGCGACGCAGAATAATGTATTTTCCAAAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTCCGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCa                                                                                                                                                                                             <  1:168664/238‑1 (MQ=255)
                                                   gtgtttGGCGCCGTAGAATTATTGTTTTCCCAAAAGGGGTTAATGGTGGTGCCCTATATGCTACCCCTTGTTACCAGGAAACCTACGACGGTTTAGTTGCCGAAGCCGAGACATTAAAGGAAAAAGACCCTGAAAATTATCAAAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAg                                                                                                                                                                                           <  2:431266/235‑1 (MQ=255)
                                                   gagaTTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCACAAAAAGATTTAGGAGCGAAACACGGGTAATACATCATCAACGGCCGTCCGGCAAGGGaaa                                                                                                                                                                                           >  2:888360/1‑238 (MQ=255)
                                                        tgggcaagaggaAAAATGGATTTTCCAAAAGGGTTTAATGGTTGGGGGTTTATTGCCCACTCAGTTTTTCAGGAAAACTACGCCTGTTTAAGTCCCGAATGCGAGACATTAAAGGGAAAAGCCCCGGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGtt                                                                                                                                                                                      <  2:1186491/229‑1 (MQ=255)
                                                        tGGCGATGACGAATAATGGTTTTCCCAAAAAGGGTAAATGGTTGGCCATTATATGCTCATCCTTGTTTTCAGGAAACCTACGGCGCTTAAGTTGCAGAAGCCGAGACATTAAAGGAAAAAGACCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGtt                                                                                                                                                                                      <  1:445113/239‑1 (MQ=255)
                                                              ggagatatattGTATTTTCCAAAAAGGTTTAAGGGTTGTGGCGTATATTCCACACCCGTTTTTAAGAAACCCTACGACGTTTTACATGCCGAAGGCGAGACATTAAAGGGAAAAGCTCCAGAAAATTACCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGct                                                                                                                                                                                 <  2:1241981/232‑1 (MQ=255)
                                                                             tttCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGGGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCCAGTCGCTAGGCGCTGGGGAAAATAAAGa                                                                                                                                                               >  1:106146/1‑239 (MQ=255)
                                                                              ttCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAATGATTGAGGAGGCAATAAAGGGCCATTCCTACTCACCGGGATTCCCGCAGGGACAGTAGCTAAGCTCTGGGCAAAATaaaa                                                                                                                                                                >  1:858903/1‑236 (MQ=255)
                                                                                                  tGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAGAGATTGCAGAGCATATCACGGGCAATCCCTCATCACCCGCCTTCCGTTCTGGCAAGTCGTTATGCGCATGGGAAAAAAAAAACATGTCCCCGGTAAAAATTgg                                                                                                                                        >  1:1170412/1‑239 (MQ=255)
                                                                                                  tGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGGGCATATCACGGGCCATCCATCATCACCGGCCTTCCGTCATGGCAAGTCGGTAGGGTCAGGGAAAAATAAAGACTGGTCACCGGGAAAAATTgg                                                                                                                                        >  1:1250172/1‑239 (MQ=255)
                                                                                                  tGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGGCCCTAAAGGGATTTAGGAGCATAACACGGTCAATCCATCATCACCGGCATTCCGTCCTGGCAAGTCGTTAGGCTCTGGGGAAAATAAAGACAGGGCACGGGGAAAAATTgg                                                                                                                                        >  1:718573/1‑239 (MQ=255)
                                                                                                   gggCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAAGGAATGAGGAGCATATCACGGTCAATCCATCATAACCGGCATTCCGCCATGGGAAGACGTTACGCTCTGGGGAAAAACAAAACTGGGAACCGGGAAAAATTTgc                                                                                                                                        >  1:476982/1‑238 (MQ=255)
                                                                                                     ggCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAATGATTGAGGAGCATATCACGGTCAATCCATTCTCACCGGCAATCCGTCCTGGCAAGTCGTTAGGGCCTTGGGAAAATAAATACTGGTCCCGGGGAAAAATTTGGt                                                                                                                                       >  1:136215/1‑239 (MQ=255)
                                                                                                       cgcTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGGCCATAAAAGGATTTAGGAGGATTTCACGGTCACTCCTTCATCCCCGGCATTCCGTCATTGGAAGACGTTGGGATCTTGGGAAAATAAAGACTGGTGCCAGGTAAAATTTtgtgtt                                                                                                                                    >  1:81048/1‑237 (MQ=255)
                                                                                                            tatGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTTCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTCGGCAAAATAAAAAACGGGACCGGGGAAAATTTTGttgtgggct                                                                                                                               >  1:271225/1‑231 (MQ=255)
                                                                                                                   accccTGTTTTCAGGAAACCTACGAGGCTTTAGTCGCCGAAGCCGGAACATTAAAGGGAAAAGGTCCTATAAATTAGCGGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGAGTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGt                                                                                                                        <  1:906502/237‑1 (MQ=255)
                                                                                                                   aTCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGGAGTTCATAAAAGGAGTGTGGGGGCAATCAAGGGTAAACCATCATCACCCGCATTCAGTCATGGAGACACGGTAGGGGCTCGGACAAAAAAAAACCGGGGCACGGGAAAATATTGTGCTGGtgggtaaggg                                                                                                                        >  2:1060007/1‑230 (MQ=255)
                                                                                                                    tCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTTCATAAAGTGATTGAGGAGCATATCACGGTCAATTCATCATTCACGGCCTTCCGTCCTGGCAACACGTTAGAACCTGGGGAAAATAAACAATAGTTACCGGGAAAATTTGCTGCTGGTCGTTTTca                                                                                                                         >  2:1020484/1‑236 (MQ=255)
                                                                                                                    tCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAGAGGTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCCTGGCAAACCGTTAGGGTCTGGGGAAAATAAAAACTGGTCACGGGGAAAATTTGTGGCTGGCCGTTATCg                                                                                                                         >  1:56109/1‑237 (MQ=255)
                                                                                                                    tCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATCAAAGGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAAGCTCTGGGAAGAATAAAAAATTGTCACGGGGAAAAATTTGTGCTGGTCGCTATTGt                                                                                                                        >  1:227035/1‑238 (MQ=255)
                                                                                                                    tCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAAGATTGAGGAGCCAAACAACGGCAATCCATCATCACCCGCATTCCCTCCTGGGAAGTCGGTAGGCCCTGGGTAAAATAAAAAATGGGCACGGGGAAAATTTTGTGGTGGTCGTTATctc                                                                                                                        >  1:1228898/1‑236 (MQ=255)
                                                                                                                    tCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGGCCATAAAATGATTGAGGAGCATATCACGGGCAATCCATCATCACCGGCATTCCGTCATGGCAAATCGTTAGGCTCTGGGGAAAATAAAAACCGGTCACGGGTAAAATTTGGTGCTGGTCGGTATCg                                                                                                                         >  1:428320/1‑237 (MQ=255)
                                                                                                                        tGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGGCCATAAAATGATTGAGGAGCATATTCCGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGGTTGGCTCTGGGAAAAAAAAAAACTGGGCACGGGGAAAAATTGTGCCTGGTCGTTATCGTCtctt                                                                                                                   >  1:411923/1‑239 (MQ=255)
                                                                                                                         gTTTTCAGGAAAACTGCGACGTGTAAGTGGCCAAAGTGAAAAAATTAAGGGAAAAAGCACAGAAAAATTAACAAAAAAAAGCCGCCACAAAGGTATTGGCGGTAGGCCAGAAAGTGATTGAGGAGCATATGACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCtcttc                                                                                                                  <  2:745652/239‑1 (MQ=255)
                                                                                                                          ttttCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTTAGGGGCATATCACGGGCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCTGTAGGCTCTGGGAAAAAAAAAGACTGGTCACGGGGAAAATTTGGTGCTGGTGTGTATCGTCccttct                                                                                                                 >  1:235274/1‑239 (MQ=255)
                                                                                                                            ttCAGAAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCttt                                                                                                               <  1:727694/239‑1 (MQ=255)
                                                                                                                                  aaaCCTTCTGCGGTTTTGTTGGCAAAGTCGCGAAAATAAAGGGAAAAAGATCTTAAAAATAATAAGAGAAAGGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTta                                                                                                          <  2:1228898/239‑1 (MQ=255)
                                                                                                                                                tttAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGGCAAACCAACATCACCGGCAATCCGGCATGGCAAGTCGTTAGGGCCTGGGGAAAAAAAAAACTGGTCACGGGGAAAAATTTGTGCTGGTGGTTATCGTCTCTTCTTTCTTTTTAGTGgaaaaagga                                                                                            >  1:305240/1‑235 (MQ=255)
                                                                                                                                                ttaaGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTa                                                                                                                                                <  1:1023653/184‑1 (MQ=255)
                                                                                                                                                 ttAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAGGCTTTAGGGGCATATCACCGTCAATCCATCATCCCCCGCATTCCGTCATGGGCAGTCGTTAGGCCCTGGGTAAAATAAAAACTGGTCACCGGTAAAATTTTGTGCTGGTGCTTTTAGTCTCTTCTTTCGTTTTAGGGCAAAACAGaa                                                                                           >  1:46945/1‑239 (MQ=255)
                                                                                                                                                      tGCAGAAGGTGAGAAATAAAAGGAAAAAGGTCTGAAAAATTAGAAGGAAAAAGCCGCCACAAAGGTATTGGCGGTAGTCCATAAAGTTATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                      <  2:106146/239‑1 (MQ=255)
                                                                                                                                                       gCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAATGATTGAGGGGCATATCACGGGCAATCCATCATCACCCGCATTCCGTCAAGGCAAGTCGTTAGGCTCTGGGAAAAAAAAAAACTGGTCACCGGGAAAATTTTGTGGCGGTCGTTTTCGTCTCTTCTTTCGTTTTAGTGAAAAAAAGAAAGccat                                                                                     >  1:401172/1‑239 (MQ=255)
                                                                                                                                                           aaggggagaatttAAGGGGAAAAGATCCTGAAATATAACAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca                                                                                      <  1:1253051/223‑1 (MQ=255)
                                                                                                                                                                  gaCATAAAAGGGAAAAGGTCATGAAAATTACAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGgga                                                                           <  1:758157/238‑1 (MQ=255)
                                                                                                                                                                          aGGTAAAAGCTCCGAAAAATTATAAAAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                                  <  1:1240155/239‑1 (MQ=255)
                                                                                                                                                                          aGGGAAAGGACCCTGAAAATTGCGAAGGAGACGCCGGTACAAAGTTATTGGCGGTAGGCCAGAAAGTCAGTGAGGATCATATCACGGTCAATCCAGCAGCGCCGGCATTCCGTCATGGCAAGTCGTTAGGCGCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTGCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGTAc                                                                  <  2:698648/239‑1 (MQ=255)
                                                                                                                                                                           aggAAAATATTCCTGTAAATTATAAAAAAAAGGCCGCCACAAAGTTATTGGCGGTAGTACATAAAGTGAATGAGGAGCATATCACGGTCAATACATCAACACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAATACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc                                                                  <  2:157233/238‑1 (MQ=255)
                                                                                                                                                                                      tCCTAAAAATAACCAAAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAaca                                                        <  1:452305/239‑1 (MQ=255)
                                                                                                                                                                                         tgaaatattaaagaaAAAACCCGCCACAAAGTTATTGGGGGTAGTCCATAAAGGGATTGAGGAGCAAATCAGCGTCAATCCATCATCACCGGCATTCCGTAATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACActc                                                     <  2:1250172/228‑1 (MQ=255)
                                                                                                                                                                                           aaaaTTTACAGAAAAAAGCCGCCACAACGTTATTGGGGGTAGTCCAAAAAGTTATTGAGGTACATATCACGGTCAAGCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTg                                                   <  2:411923/239‑1 (MQ=255)
                                                                                                                                                                                           aaaaTTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTg                                                   <  1:888360/239‑1 (MQ=255)
                                                                                                                                                                                                    ggaaaagaGCCGCCACAAAGTTAGTGGCGGTAGGCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCAACGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                          <  1:35882/232‑1 (MQ=255)
                                                                                                                                                                                                    agaaAAATGCCTCCAAAAATTTTATTGCGGTAGGCCATAACGTGATTGAGGAGCAAAACACGGTCAATCCATCATCACCGGCATTCCGTTATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGTTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                          <  2:1195701/239‑1 (MQ=255)
                                                                                                                                                                                                    agaaAAAAGCCGCCACAAAGGTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTAc                                          <  1:426826/239‑1 (MQ=255)
                                                                                                                                                                                                                tctaaatgTTATTAGCTTTTGTCCAAAAAGTGATTGAGGATCTTGTCCAGGTTAGTACATCAACATCAGTAATCCGTTATTGCAAGTCTGTATGCACGGGGAAACATAAAGGCTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTGCTTCTTTAGTTATAGTGAAAAAGAGAAAGTAATCATTCTAGTATGGATGAACGATGACAACACTCTGCGCACCTACGGTAAAAAAACa                              <  2:593562/232‑1 (MQ=255)
                                                                                                                                                                                                                ccACAAATTTAGTGGCGGTAGTCCATAAAGTTGTTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGGCTGGTCACGGGTAAAATTTTGTGCTTGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                              <  1:311065/239‑1 (MQ=255)
                                                                                                                                                                                                                ccAAAAAGTTTTTGGCGGTAGTCCAAAAAGTGATTGAGGAGCAGATCCAGGTAAATCCAACATCACCGGACTTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                              <  2:305240/239‑1 (MQ=255)
                                                                                                                                                                                                                cacaaaaGTTTTTGGGGGTATTCCAAAAAGGGTTTGGGGGTAATTTCCAGGTCAAACCAACAACACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                              <  2:56109/235‑1 (MQ=255)
                                                                                                                                                                                                                 caaaaaGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                              <  1:1020484/235‑1 (MQ=255)
                                                                                                                                                                                                                  acaAAGTTTGTGGCGGTAGTCCATAAAGTGATTGAGGGGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTGTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                            <  2:136215/239‑1 (MQ=255)
                                                                                                                                                                                                                  acaAAGTTATTTGCGGTAGTCCATAAAGTGATTGAGGAGCATATCCCGGTCAATCCATCATCACCGGCATTCCGTCATGGAAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCGCTTCTTTCGTTAGAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                            <  2:401172/239‑1 (MQ=255)
                                                                                                                                                                                                                  acaAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                            <  1:485986/239‑1 (MQ=255)
                                                                                                                                                                                                                  aacaatttATTTGCCGTTGCCCATAAATTGAGTGAGGACCATTTCACCGGCAATCCATCATACCCGCGATGCCGGCATGGCAAGGCTTTAGTGTGTGGGAAAAATAAAGCCCGGTCACGGGTAAAATTTGGTTCTGGTCGTTATCGTCTCTTCTTTCTTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGTTAAAAAAACAGa                            <  2:858903/233‑1 (MQ=255)
                                                                                                                                                                                                                   caAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGAt                           <  1:1060007/239‑1 (MQ=255)
                                                                                                                                                                                                                     aatttAGTGGCGGTAGGCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                         <  1:530624/236‑1 (MQ=255)
                                                                                                                                                                                                                     aaGTTTTTGGCGGTTGTCCAATAAGGGATTGAGGGGCATATCACGGGTAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                         <  2:428320/239‑1 (MQ=255)
                                                                                                                                                                                                                     aaGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                         <  1:537623/239‑1 (MQ=255)
                                                                                                                                                                                                                        ttATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCt                       <  1:1038664/238‑1 (MQ=255)
                                                                                                                                                                                                                         tATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCt                       <  1:58331/237‑1 (MQ=255)
                                                                                                                                                                                                                                      ccATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTGCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa                            <  1:1247036/219‑1 (MQ=255)
                                                                                                                                                                                                                                      ccATAAAGGGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTTATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata                    <  1:1061843/227‑1 (MQ=255)
                                                                                                                                                                                                                                        cttaaGTGATTTGGGGGGATATCGCGGGAAATGCATCATAACCGGGATTCCGTCATGGCAAGGTGGTAGGGTCTGGGAAAAATAAAGGCTGGTAAAGGGTAAAATTTGGTGCTGGTCGGTAGCGTCACTTCCTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCGGCGCACCTACGGTAAAAAAACAGATGCCTATCACGTATTCAGCaaa      <  2:356710/236‑1 (MQ=255)
                                                                                                                                                                                                                                        aTAAAGTGATTGAGGAGCATATCCCGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCaaa      <  1:125034/239‑1 (MQ=255)
                                                                                                                                                                                                                                        aTAAAGTGATTGAGGAGCATAACACGGTCAATCCATCATCACCGGCATTCCGGCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCaaa      <  1:640612/239‑1 (MQ=255)
                                                                                                                                                                                                                                          aaaGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAAt    >  1:721720/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACCAGATGCCTATCCCGTATTCAGCAAAATGt  >  1:213305/1‑239 (MQ=255)
                                                                                                                                                                                                                                              tGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCATCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc                         <  1:511664/214‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                
ATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAATAAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT  >  NC_000913/3277266‑3277736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: